Restriction map for
pFN21AB6387
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
DsaI Eam1105I EarI Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
SacI SacII SalI SapI SbfI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 780 GACGTC GACGT^C
AcyI 780 GACGTC G[GA]^CG[CT]C
AfaI 67 GTAC GT^AC
AvaI 87 CCCGGG C^[CT]CG[GA]G
BalI 492 TGGCCA TGG^CCA
BanII 268 GAGCCC G[GA]GC[CT]^C
BsaHI 780 GACGTC G[GA]^CG[CT]C
BsaI 341 GAGACC GGTCTC(1/5)
BseRI 94 GAGGAG GAGGAG(10/8)
BsmAI 341 GAGAC GTCTC(1/5)
BsmFI 224 GGGAC GGGAC(10/14)
BsmI 165 GAATGC GAATGC(1/-1)
BsoBI 87 CCCGGG C^[CT]CG[GA]G
BstEII 237 GGTCACC G^GT[ACGT]ACC
BstNI 133 CCTGG CC^[AT]GG
BstPI 237 GGTCACC G^GT[ACGT]ACC
CpoI 201 CGGTCCG CG^G[AT]CCG
DdeI 311 CTCAG C^T[ACGT]AG
DraI 598 TTTAAA TTT^AAA
DraII 129 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 240 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 581 GACAGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 249 CGGCCG C^GGCCG
EclXI 249 CGGCCG C^GGCCG
Eco52I 249 CGGCCG C^GGCCG
EcoO109I 129 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 237 GGTCACC G^GT[ACGT]ACC
EcoRII 133 CCTGG ^CC[AT]GG
HaeII 215 GGCGCT [GA]GCGC^[CT]
Hin1I 780 GACGTC G[GA]^CG[CT]C
HindIII 764 AAGCTT A^AGCTT
MaeII 781 ACGT A^CGT
MluNI 492 TGGCCA TGG^CCA
MscI 492 TGGCCA TGG^CCA
MvaI 133 CCTGG CC^[AT]GG
NotI 248 GCGGCCGC GC^GGCCGC
PflMI 495 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 546 CAGCTG CAG^CTG
RsaI 67 GTAC GT^AC
RsrII 201 CGGTCCG CG^G[AT]CCG
ScaI 66 AGTACT AGT^ACT
SmaI 87 CCCGGG CCC^GGG
TfiI 28 GAATC G^A[AT]TC
Van91I 495 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 87 CCCGGG C^CCGGG
XmaI 87 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 82 GTGCTC G[AT]GC[AT]^C
177 GTGCTC
AvaII 202 GGTCC G^G[AT]CC
225 GGACC
BfaI 70 CTAG C^TAG
170 CTAG
BglII 480 AGATCT A^GATCT
800 AGATCT
BsiEI 151 CGACCG CG[GA][CT]^CG
249 CGGCCG
BsiHKAI 82 GTGCTC G[AT]GC[AT]^C
177 GTGCTC
BspMI 9 ACCTGC ACCTGC(4/8)
471 GCAGGT
BssHII 49 GCGCGC G^CGCGC
51 GCGCGC
BstF5I 729 CATCC GGATG(2/0)
585 GGATG
BstXI 345 CCATGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
560 CCAATGGAATGG
BstYI 480 AGATCT [GA]^GATC[CT]
800 AGATCT
FauI 37 GCGGG CCCGC(4/6)
183 GCGGG
FokI 729 CATCC GGATG(9/13)
585 GGATG
HgaI 121 GACGC GACGC(5/10)
13 GCGTC
HphI 91 GGTGA GGTGA(8/7)
239 TCACC
MaeI 70 CTAG C^TAG
170 CTAG
MboII 704 GAAGA GAAGA(8/7)
803 TCTTC
MflI 480 AGATCT [GA]^GATC[CT]
800 AGATCT
MspA1I 282 CAGCGG C[AC]G^C[GT]G
546 CAGCTG
PleI 307 GACTC GAGTC(4/5)
606 GACTC
XhoII 480 AGATCT [GA]^GATC[CT]
800 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BmyI BpmI BsaJI BsgI BsiYI
BslI Bsp1286I BsrI BssKI BstUI
Cac8I CfoI Cfr13I DpnII EaeI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeIII MboI
MnlI MseI MspI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI Tru9I TseI Tsp45I
Tsp509I TspRI