(Circular) (Six-base) MAPSORT of: pFN21AB6387.seq Check: 5923 from: 1 to: 5530 pFN21AB6387 5530 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2789 3340 278 Size: 53 83 186 936 1253 551 2468 Fragments arranged by size: 2468 1253 936 551 186 83 53 AccI GT'mk_AC Cuts at: 1937 2873 1937 Size: 936 4594 Acc65I G'GTAC_C Cuts at: 2851 2851 Size: 5530 AclI AA'CG_TT Cuts at: 1563 3657 4030 1563 Size: 2094 373 3063 Fragments arranged by size: 3063 2094 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3593 4977 5245 1632 Size: 1961 1384 268 1917 Fragments arranged by size: 1961 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5333 AflIII A'CryG_T Cuts at: 1203 4429 5406 1203 Size: 3226 977 1327 Fragments arranged by size: 3226 1327 977 AgeI A'CCGG_T Cuts at: 4361 4361 Size: 5530 AhdI GACnn_n'nnGTC Cuts at: 4259 4259 Size: 5530 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5530 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5530 AlwNI CAG_nnn'CTG Cuts at: 3273 4845 3273 Size: 1572 3958 ApaLI G'TGCA_C Cuts at: 3587 4743 3587 Size: 1156 4374 ApoI r'AATT_y Cuts at: 1493 2839 3065 3217 5326 1493 Size: 1346 226 152 2109 1697 Fragments arranged by size: 2109 1697 1346 226 152 AseI AT'TA_AT Cuts at: 160 1032 4084 160 Size: 872 3052 1606 Fragments arranged by size: 3052 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 AvaI C'yCGr_G Cuts at: 1870 1943 2092 2855 1870 Size: 73 149 763 4545 Fragments arranged by size: 4545 763 149 73 BamHI G'GATC_C Cuts at: 2860 3227 2860 Size: 367 5163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2851 4306 618 Size: 325 217 627 1064 1455 1842 Fragments arranged by size: 1842 1455 1064 627 325 217 BanII G_rGCy'C Cuts at: 1391 2277 2849 1391 Size: 886 572 4072 Fragments arranged by size: 4072 886 572 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4559 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3721 3755 1903 Size: 34 1784 34 3678 Fragments arranged by size: 3678 1784 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3422 4632 3422 Size: 1210 4320 BclI T'GATC_A Cuts at: 1548 1548 Size: 5530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4141 136 Size: 107 122 71 631 605 2469 1525 Fragments arranged by size: 2469 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2485 2805 5525 2485 Size: 320 2720 2490 Fragments arranged by size: 2720 2490 320 BlpI GC'TnA_GC Cuts at: 2947 2947 Size: 5530 Bme1580I G_kGCm'C Cuts at: 1790 3591 4747 1790 Size: 1801 1156 2573 Fragments arranged by size: 2573 1801 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5530 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 3261 4219 Size: 416 53 83 399 24 897 947 958 Cuts at: 4219 442 Size: 1753 Fragments arranged by size: 1753 958 947 897 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 2625 2705 2797 4190 1239 Size: 1161 225 80 92 1393 2579 Fragments arranged by size: 2579 1393 1161 225 92 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3655 4520 4818 3655 Size: 865 298 4367 Fragments arranged by size: 4367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2340 4193 882 Size: 617 841 1853 2219 Fragments arranged by size: 2219 1853 841 617 BsaAI yAC'GTr Cuts at: 493 1204 3321 493 Size: 711 2117 2702 Fragments arranged by size: 2702 2117 711 BsaBI GATnn'nnATC Cuts at: 5524 5524 Size: 5530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2786 3337 3719 4354 Size: 53 83 186 936 1253 551 382 635 Cuts at: 4354 275 Size: 1451 Fragments arranged by size: 1451 1253 936 635 551 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3963 4361 4635 4782 5193 3963 Size: 398 274 147 411 4300 Fragments arranged by size: 4300 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 2337 2367 1178 Size: 30 165 30 741 30 163 30 4341 Fragments arranged by size: 4341 741 165 163 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 1186 Size: 928 4602 BseYI C'CCAG_C Cuts at: 4733 5229 4733 Size: 496 5034 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2046 2166 2495 1163 Size: 573 310 120 329 4198 Fragments arranged by size: 4198 573 329 310 120 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2159 2257 2980 3741 4769 1540 Size: 275 186 158 98 723 761 1028 2301 Fragments arranged by size: 2301 1028 761 723 275 186 158 98 BsiHKAI G_wGCw'C Cuts at: 2091 2186 3591 3676 4747 2091 Size: 95 1405 85 1071 2874 Fragments arranged by size: 2874 1405 1071 95 85 BsmI GAATG_Cn' Cuts at: 2176 3041 3134 2176 Size: 865 93 4572 Fragments arranged by size: 4572 865 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2186 2277 2849 3591 3676 4747 Size: 399 301 95 91 572 742 85 1071 Cuts at: 4747 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 572 399 301 95 91 85 BspEI T'CCGG_A Cuts at: 5193 5193 Size: 5530 BspHI T'CATG_A Cuts at: 3419 5149 3419 Size: 1730 3800 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2023 2467 2885 844 Size: 1179 444 418 3489 Fragments arranged by size: 3489 1179 444 418 BsrBI CCG'CTC Cuts at: 1394 1823 3417 1394 Size: 429 1594 3507 Fragments arranged by size: 3507 1594 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4025 4199 65 Size: 1183 2777 174 1396 Fragments arranged by size: 2777 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4174 4361 1700 Size: 110 2364 187 2869 Fragments arranged by size: 2869 2364 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5530 BssHII G'CGCG_C Cuts at: 1925 2054 2056 1925 Size: 129 2 5399 Fragments arranged by size: 5399 129 2 BssSI C'ACGA_G Cuts at: 3590 4602 3590 Size: 1012 4518 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5530 BstBI TT'CG_AA Cuts at: 4334 4334 Size: 5530 BstEII G'GTnAC_C Cuts at: 1184 2242 1184 Size: 1058 4472 BstXI CCAn_nnnn'nTGG Cuts at: 2357 2572 2357 Size: 215 5315 BstYI r'GATC_y Cuts at: 1972 2485 2805 2860 3227 3612 3629 5081 5167 Size: 513 320 55 367 385 17 1452 86 Cuts at: 5167 5179 5525 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 513 385 367 346 320 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4039 Fragments arranged by size: 4039 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4791 Fragments arranged by size: 4791 683 56 BtsI GCAGTG_nn' Cuts at: 2154 2287 3046 3840 3860 2154 Size: 133 759 794 20 3824 Fragments arranged by size: 3824 794 759 133 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5495 DraI TTT'AAA Cuts at: 2605 2834 2605 Size: 229 5301 DraIII CAC_nnn'GTG Cuts at: 2250 2250 Size: 5530 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2592 4537 809 Size: 507 1276 1945 1802 Fragments arranged by size: 1945 1802 1276 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2254 2497 Size: 54 1425 50 275 18 177 247 243 Cuts at: 2497 2977 3866 5495 8 Size: 480 889 1629 43 Fragments arranged by size: 1629 1425 889 480 275 247 243 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2254 2977 1537 Size: 275 442 723 4090 Fragments arranged by size: 4090 723 442 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3460 1353 Size: 2107 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4117 4491 4637 387 Size: 1064 138 2528 374 146 1280 Fragments arranged by size: 2528 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2400 2625 2705 2797 3593 4190 4977 Size: 393 768 225 80 92 796 597 787 Cuts at: 4977 5245 1239 Size: 268 1524 Fragments arranged by size: 1524 796 787 768 597 393 268 225 92 80 EcoO109I rG'GnC_Cy Cuts at: 2135 2135 Size: 5530 EcoRI G'AATT_C Cuts at: 2839 3217 2839 Size: 378 5152 FspI TGC'GCA Cuts at: 4036 4036 Size: 5530 HaeII r_GCGC'y Cuts at: 1678 2224 4677 1678 Size: 546 2453 2531 Fragments arranged by size: 2531 2453 546 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2475 2507 2776 2808 4171 Size: 32 135 32 903 32 269 32 1363 Cuts at: 4171 4203 4245 4277 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1363 903 269 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2874 3120 669 Size: 988 281 936 246 3079 Fragments arranged by size: 3079 988 936 281 246 HindIII A'AGCT_T Cuts at: 748 2769 748 Size: 2021 3509 HpaI GTT'AAC Cuts at: 3120 3120 Size: 5530 KpnI G_GTAC'C Cuts at: 2855 2855 Size: 5530 MfeI C'AATT_G Cuts at: 3129 3129 Size: 5530 MluI A'CGCG_T Cuts at: 5406 5406 Size: 5530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2383 2461 4644 4828 1305 Size: 1078 78 2183 184 2007 Fragments arranged by size: 2183 2007 1078 184 78 MscI TGG'CCA Cuts at: 10 64 1489 1832 2499 10 Size: 54 1425 343 667 3041 Fragments arranged by size: 3041 1425 667 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3488 3847 4006 518 Size: 648 2322 359 159 2042 Fragments arranged by size: 2322 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2289 2553 2946 3623 4771 5016 1606 Size: 683 264 393 677 1148 245 2120 Fragments arranged by size: 2120 1148 683 677 393 264 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4039 Fragments arranged by size: 4039 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5530 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5530 NotI GC'GGCC_GC Cuts at: 2254 2977 2254 Size: 723 4807 NspI r_CATG'y Cuts at: 1132 1229 2888 4433 1132 Size: 97 1659 1545 2229 Fragments arranged by size: 2229 1659 1545 97 PasI CC'CwG_GG Cuts at: 1348 2138 1348 Size: 790 4740 PciI A'CATG_T Cuts at: 4429 4429 Size: 5530 PflMI CCAn_nnn'nTGG Cuts at: 1108 2506 1108 Size: 1398 4132 PmeI GTTT'AAAC Cuts at: 2834 2834 Size: 5530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3631 5112 5144 3599 Size: 32 1481 32 3985 Fragments arranged by size: 3985 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5530 PsiI TTA'TAA Cuts at: 3100 3100 Size: 5530 PstI C_TGCA'G Cuts at: 830 2882 830 Size: 2052 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5530 PvuII CAG'CTG Cuts at: 2553 2553 Size: 5530 RsrII CG'GwC_CG Cuts at: 2207 2207 Size: 5530 SalI G'TCGA_C Cuts at: 1936 2872 1936 Size: 936 4594 SbfI CC_TGCA'GG Cuts at: 2882 2882 Size: 5530 ScaI AGT'ACT Cuts at: 2073 3778 5490 2073 Size: 1705 1712 2113 Fragments arranged by size: 2113 1712 1705 SfcI C'TryA_G Cuts at: 826 1045 2878 4013 4694 4885 826 Size: 219 1833 1135 681 191 1471 Fragments arranged by size: 1833 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5530 SmaI CCC'GGG Cuts at: 1872 2094 2857 1872 Size: 222 763 4545 Fragments arranged by size: 4545 763 222 SmlI C'TyrA_G Cuts at: 820 1017 1943 3634 4535 4797 820 Size: 197 926 1691 901 262 1553 Fragments arranged by size: 1691 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3321 493 Size: 2828 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5530 SphI G_CATG'C Cuts at: 1132 2888 1132 Size: 1756 3774 SspI AAT'ATT Cuts at: 5 52 3454 5 Size: 47 3402 2081 Fragments arranged by size: 3402 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5530 StyI C'CwwG_G Cuts at: 513 1065 2004 2969 513 Size: 552 939 965 3074 Fragments arranged by size: 3074 965 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2296 2564 3557 3574 3727 3912 4357 Size: 1255 716 268 993 17 153 185 445 Cuts at: 4357 325 Size: 1498 Fragments arranged by size: 1498 1255 993 716 445 268 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2071 3776 5488 Size: 274 80 33 51 1443 94 1705 1712 Cuts at: 5488 96 Size: 138 Fragments arranged by size: 1712 1705 1443 274 138 94 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2337 2369 5490 5522 2337 Size: 32 3121 32 2345 Fragments arranged by size: 3121 2345 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5530 XbaI T'CTAG_A Cuts at: 2866 2866 Size: 5530 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5530 XmaI C'CCGG_G Cuts at: 1870 2092 2855 1870 Size: 222 763 4545 Fragments arranged by size: 4545 763 222 XmnI GAAnn'nnTTC Cuts at: 3659 3659 Size: 5530 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2787 3338 276 Size: 53 83 186 936 1253 551 2468 Fragments arranged by size: 2468 1253 936 551 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI