(Circular) (Six-base) MAPSORT of: pFN21AB6373.seq Check: 1240 from: 1 to: 5515 pFN21AB6373 5515 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2858 1937 Size: 921 4594 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3578 4962 5230 1632 Size: 1946 1384 268 1917 Fragments arranged by size: 1946 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 4414 5391 1203 Size: 3211 977 1327 Fragments arranged by size: 3211 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 2789 1166 Size: 1623 3892 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2747 1184 Size: 1563 3952 AlwNI CAG_nnn'CTG Cuts at: 2335 2647 3258 4830 2335 Size: 312 611 1572 3020 Fragments arranged by size: 3020 1572 611 312 ApaI G_GGCC'C Cuts at: 2107 2149 2107 Size: 42 5473 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2824 3050 3202 5311 1493 Size: 1331 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2106 2840 1870 Size: 73 163 734 4545 Fragments arranged by size: 4545 734 163 73 AvrII C'CTAG_G Cuts at: 2609 2609 Size: 5515 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2836 4291 618 Size: 325 217 627 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 627 325 217 BanII G_rGCy'C Cuts at: 1391 2107 2149 2163 2834 1391 Size: 716 42 14 671 4072 Fragments arranged by size: 4072 716 671 42 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2219 928 Size: 971 320 4224 Fragments arranged by size: 4224 971 320 BbvCI CC'TCA_GC Cuts at: 2670 2670 Size: 5515 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3706 3740 1903 Size: 34 1769 34 3678 Fragments arranged by size: 3678 1769 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 1548 Size: 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2244 2658 4126 Size: 107 122 71 631 605 572 414 1468 Cuts at: 4126 136 Size: 1525 Fragments arranged by size: 1525 1468 631 605 572 414 122 107 71 BglII A'GATC_T Cuts at: 5510 5510 Size: 5515 BlpI GC'TnA_GC Cuts at: 2932 2932 Size: 5515 Bme1580I G_kGCm'C Cuts at: 1790 2107 2149 3576 4732 1790 Size: 317 42 1427 1156 2573 Fragments arranged by size: 2573 1427 1156 317 42 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2360 2657 3246 Size: 416 53 83 399 24 943 297 589 Cuts at: 3246 4204 442 Size: 958 1753 Fragments arranged by size: 1753 958 943 589 416 399 297 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2326 2326 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2187 2357 2407 4175 1239 Size: 932 16 170 50 1768 2579 Fragments arranged by size: 2579 1768 932 170 50 16 Bpu10I CC'TnA_GC Cuts at: 2670 2670 Size: 5515 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3640 4505 4803 3640 Size: 865 298 4352 Fragments arranged by size: 4352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2222 2300 4178 882 Size: 617 723 78 1878 2219 Fragments arranged by size: 2219 1878 723 617 78 BsaAI yAC'GTr Cuts at: 493 1204 3306 493 Size: 711 2102 2702 Fragments arranged by size: 2702 2102 711 BsaBI GATnn'nnATC Cuts at: 5509 5509 Size: 5515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2796 3322 3704 4339 Size: 53 83 186 936 1263 526 382 635 Cuts at: 4339 275 Size: 1451 Fragments arranged by size: 1451 1263 936 635 526 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2534 2564 1178 Size: 30 165 30 1131 30 4129 Fragments arranged by size: 4129 1131 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2318 2597 1186 Size: 1132 279 4104 Fragments arranged by size: 4104 1132 279 BseYI C'CCAG_C Cuts at: 2072 2640 2755 4718 5214 2072 Size: 568 115 1963 496 2373 Fragments arranged by size: 2373 1963 568 496 115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2701 2769 1163 Size: 573 965 68 3909 Fragments arranged by size: 3909 965 573 68 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3726 4754 1540 Size: 275 186 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2163 2786 3576 3661 4732 2163 Size: 623 790 85 1071 2946 Fragments arranged by size: 2946 1071 790 623 85 BsmI GAATG_Cn' Cuts at: 3026 3119 3026 Size: 93 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2149 2163 2786 2834 3576 3661 Size: 399 317 42 14 623 48 742 85 Cuts at: 3661 4732 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 623 399 317 85 48 42 14 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 3404 5134 3404 Size: 1730 3785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2397 2870 844 Size: 1553 473 3489 Fragments arranged by size: 3489 1553 473 BsrBI CCG'CTC Cuts at: 1394 1823 3402 1394 Size: 429 1579 3507 Fragments arranged by size: 3507 1579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4010 4184 65 Size: 1183 2762 174 1396 Fragments arranged by size: 2762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4159 4346 1700 Size: 110 2349 187 2869 Fragments arranged by size: 2869 2349 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 2083 1925 Size: 158 5357 BssSI C'ACGA_G Cuts at: 2735 3575 4587 2735 Size: 840 1012 3663 Fragments arranged by size: 3663 1012 840 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 4319 4319 Size: 5515 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2506 2431 Size: 75 5440 BstYI r'GATC_y Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510 Size: 873 367 385 17 1452 86 12 346 Cuts at: 5510 1972 Size: 1977 Fragments arranged by size: 1977 1452 873 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2301 2301 Size: 5515 BtgI C'CryG_G Cuts at: 513 1065 2004 2790 513 Size: 552 939 786 3238 Fragments arranged by size: 3238 939 786 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 2694 3031 3825 3845 2694 Size: 337 794 20 4364 Fragments arranged by size: 4364 794 337 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2245 2275 2443 Size: 54 1425 50 275 18 415 30 168 Cuts at: 2443 2469 2962 3851 5480 8 Size: 26 493 889 1629 43 Fragments arranged by size: 1629 1425 889 493 415 275 168 54 50 43 30 26 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2390 3445 1353 Size: 1037 1055 3423 Fragments arranged by size: 3423 1055 1037 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2225 2315 4102 4476 4622 387 Size: 1064 138 636 90 1787 374 146 1280 Fragments arranged by size: 1787 1280 1064 636 374 146 138 90 EcoICRI GAG'CTC Cuts at: 2161 2161 Size: 5515 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2187 2357 2407 3578 4175 4962 Size: 393 539 16 170 50 1171 597 787 Cuts at: 4962 5230 1239 Size: 268 1524 Fragments arranged by size: 1524 1171 787 597 539 393 268 170 50 16 EcoNI CCTnn'n_nnAGG Cuts at: 2153 2153 Size: 5515 EcoO109I rG'GnC_Cy Cuts at: 2103 2146 2103 Size: 43 5472 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 EcoRV GAT'ATC Cuts at: 2806 2806 Size: 5515 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 4662 1678 Size: 2984 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2061 2093 2100 2132 4156 Size: 32 135 32 489 32 7 32 2024 Cuts at: 4156 4188 4230 4262 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2024 489 135 42 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2266 2542 2859 3105 669 Size: 988 281 328 276 317 246 3079 Fragments arranged by size: 3079 988 328 317 281 276 246 HindIII A'AGCT_T Cuts at: 748 2502 748 Size: 1754 3761 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 3114 3114 Size: 5515 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2759 4629 4813 1305 Size: 1454 1870 184 2007 Fragments arranged by size: 2007 1870 1454 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2277 2471 10 Size: 54 1425 343 445 194 3054 Fragments arranged by size: 3054 1425 445 343 194 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 3473 3832 3991 518 Size: 648 1623 684 359 159 2042 Fragments arranged by size: 2042 1623 684 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2100 2332 2575 2669 2931 3608 4756 5001 Size: 494 232 243 94 262 677 1148 245 Cuts at: 5001 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 494 262 245 243 232 94 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4024 Fragments arranged by size: 4024 939 552 NdeI CA'TA_TG Cuts at: 387 2527 387 Size: 2140 3375 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2635 2873 4418 1132 Size: 97 1406 238 1545 2229 Fragments arranged by size: 2229 1545 1406 238 97 PasI CC'CwG_GG Cuts at: 1348 2317 1348 Size: 969 4546 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 2647 1108 Size: 1227 312 3976 Fragments arranged by size: 3976 1227 312 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PspOMI G'GGCC_C Cuts at: 2103 2145 2103 Size: 42 5473 PstI C_TGCA'G Cuts at: 830 2867 830 Size: 2037 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 PvuII CAG'CTG Cuts at: 2332 2332 Size: 5515 SacI G_AGCT'C Cuts at: 2163 2163 Size: 5515 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SfcI C'TryA_G Cuts at: 826 1045 2863 3998 4679 4870 826 Size: 219 1818 1135 681 191 1471 Fragments arranged by size: 1818 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2658 2658 Size: 5515 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2842 1872 Size: 970 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3619 4520 4782 820 Size: 197 926 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2635 2873 1132 Size: 1503 238 3774 Fragments arranged by size: 3774 1503 238 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 2299 1517 Size: 782 4733 StyI C'CwwG_G Cuts at: 513 1065 2004 2475 2609 2954 513 Size: 552 939 471 134 345 3074 Fragments arranged by size: 3074 939 552 471 345 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2298 3542 3559 3712 3897 4342 325 Size: 1255 718 1244 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1244 718 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3761 5473 96 Size: 274 80 33 51 1443 1784 1712 138 Fragments arranged by size: 1784 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5475 5507 5475 Size: 32 5483 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2562 2562 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2840 1870 Size: 970 4545 XmnI GAAnn'nnTTC Cuts at: 3644 3644 Size: 5515 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI