(Circular) (Six-base) MAPSORT of: pFN21AB6368.seq Check: 3015 from: 1 to: 5599 pFN21AB6368 5599 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2194 2194 Size: 5599 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3409 278 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2484 2942 1937 Size: 547 458 4594 Fragments arranged by size: 4594 547 458 Acc65I G'GTAC_C Cuts at: 2920 2920 Size: 5599 AclI AA'CG_TT Cuts at: 1563 3726 4099 1563 Size: 2163 373 3063 Fragments arranged by size: 3063 2163 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2244 3662 5046 5314 1632 Size: 612 1418 1384 268 1917 Fragments arranged by size: 1917 1418 1384 612 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5402 AflIII A'CryG_T Cuts at: 1203 4498 5475 1203 Size: 3295 977 1327 Fragments arranged by size: 3295 1327 977 AgeI A'CCGG_T Cuts at: 4430 4430 Size: 5599 AhdI GACnn_n'nnGTC Cuts at: 4328 4328 Size: 5599 AleI CACnn'nnGTG Cuts at: 1166 2393 1166 Size: 1227 4372 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2081 2555 1184 Size: 897 474 4228 Fragments arranged by size: 4228 897 474 AlwNI CAG_nnn'CTG Cuts at: 3342 4914 3342 Size: 1572 4027 ApaLI G'TGCA_C Cuts at: 3656 4812 3656 Size: 1156 4443 ApoI r'AATT_y Cuts at: 1493 2663 2908 3134 3286 5395 1493 Size: 1170 245 226 152 2109 1697 Fragments arranged by size: 2109 1697 1170 245 226 152 AseI AT'TA_AT Cuts at: 160 1032 4153 160 Size: 872 3121 1606 Fragments arranged by size: 3121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 AvaI C'yCGr_G Cuts at: 1870 1943 2924 1870 Size: 73 981 4545 Fragments arranged by size: 4545 981 73 BamHI G'GATC_C Cuts at: 2929 3296 2929 Size: 367 5232 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2920 4375 618 Size: 325 217 627 1133 1455 1842 Fragments arranged by size: 1842 1455 1133 627 325 217 BanII G_rGCy'C Cuts at: 1391 2529 2709 2918 1391 Size: 1138 180 209 4072 Fragments arranged by size: 4072 1138 209 180 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2196 2288 2388 928 Size: 971 297 92 100 4139 Fragments arranged by size: 4139 971 297 100 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3790 3824 1903 Size: 34 1853 34 3678 Fragments arranged by size: 3678 1853 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3491 4701 3491 Size: 1210 4389 BclI T'GATC_A Cuts at: 1548 1548 Size: 5599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4210 136 Size: 107 122 71 631 605 2538 1525 Fragments arranged by size: 2538 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5594 5594 Size: 5599 BlpI GC'TnA_GC Cuts at: 3016 3016 Size: 5599 Bme1580I G_kGCm'C Cuts at: 1790 2571 2857 3660 4816 1790 Size: 781 286 803 1156 2573 Fragments arranged by size: 2573 1156 803 781 286 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5599 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3330 4288 442 Size: 416 53 83 399 24 1913 958 1753 Fragments arranged by size: 1913 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5599 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2536 2536 Size: 5599 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2292 2458 4259 1239 Size: 1053 166 1801 2579 Fragments arranged by size: 2579 1801 1053 166 Bpu10I CC'TnA_GC Cuts at: 2102 2102 Size: 5599 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2843 3724 4589 4887 2843 Size: 881 865 298 3555 Fragments arranged by size: 3555 881 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4262 882 Size: 617 2763 2219 Fragments arranged by size: 2763 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3390 493 Size: 711 2186 2702 Fragments arranged by size: 2702 2186 711 BsaBI GATnn'nnATC Cuts at: 5593 5593 Size: 5599 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3406 3788 4423 275 Size: 53 83 186 936 1873 382 635 1451 Fragments arranged by size: 1873 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4032 4430 4704 4851 5262 4032 Size: 398 274 147 411 4369 Fragments arranged by size: 4369 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2334 2364 1178 Size: 30 165 30 931 30 4413 Fragments arranged by size: 4413 931 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5599 BseYI C'CCAG_C Cuts at: 2127 2140 2338 2770 2799 4802 5298 2127 Size: 13 198 432 29 2003 496 2428 Fragments arranged by size: 2428 2003 496 432 198 29 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2027 1163 Size: 573 291 4735 Fragments arranged by size: 4735 573 291 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2786 3049 3810 4838 1540 Size: 275 186 785 263 761 1028 2301 Fragments arranged by size: 2301 1028 785 761 275 263 186 BsiHKAI G_wGCw'C Cuts at: 2108 2529 2709 2898 3660 3745 4816 2108 Size: 421 180 189 762 85 1071 2891 Fragments arranged by size: 2891 1071 762 421 189 180 85 BsmI GAATG_Cn' Cuts at: 3110 3203 3110 Size: 93 5506 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2529 2571 2709 2857 2898 2918 Size: 399 318 421 42 138 148 41 20 Cuts at: 2918 3660 3745 4816 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 421 399 318 148 138 85 42 41 20 BspEI T'CCGG_A Cuts at: 5262 5262 Size: 5599 BspHI T'CATG_A Cuts at: 3488 5218 3488 Size: 1730 3869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2194 2954 844 Size: 1350 760 3489 Fragments arranged by size: 3489 1350 760 BsrBI CCG'CTC Cuts at: 1394 1823 2868 3486 1394 Size: 429 1045 618 3507 Fragments arranged by size: 3507 1045 618 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4094 4268 65 Size: 1183 2846 174 1396 Fragments arranged by size: 2846 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 2785 4243 4430 1700 Size: 110 236 739 1458 187 2869 Fragments arranged by size: 2869 1458 739 236 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5599 BssHII G'CGCG_C Cuts at: 1925 2803 1925 Size: 878 4721 BssSI C'ACGA_G Cuts at: 2579 3659 4671 2579 Size: 1080 1012 3507 Fragments arranged by size: 3507 1080 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5599 BstBI TT'CG_AA Cuts at: 4403 4403 Size: 5599 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5599 BstXI CCAn_nnnn'nTGG Cuts at: 2093 2199 2395 2093 Size: 106 196 5297 Fragments arranged by size: 5297 196 106 BstYI r'GATC_y Cuts at: 1972 2266 2929 3296 3681 3698 5150 5236 5248 Size: 294 663 367 385 17 1452 86 12 Cuts at: 5248 5594 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 663 385 367 346 294 86 17 12 BstZ17I GTA'TAC Cuts at: 2485 2485 Size: 5599 BtgI C'CryG_G Cuts at: 513 1065 2078 513 Size: 552 1013 4034 Fragments arranged by size: 4034 1013 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2476 487 Size: 683 56 1250 3610 Fragments arranged by size: 3610 1250 683 56 BtsI GCAGTG_nn' Cuts at: 2063 2473 3115 3909 3929 2063 Size: 410 642 794 20 3733 Fragments arranged by size: 3733 794 642 410 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5564 DraI TTT'AAA Cuts at: 2903 2903 Size: 5599 DraIII CAC_nnn'GTG Cuts at: 2698 2698 Size: 5599 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4606 809 Size: 507 3290 1802 Fragments arranged by size: 3290 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2292 2700 Size: 54 1425 50 275 18 265 197 408 Cuts at: 2700 2757 2783 2787 2889 3046 3935 5564 8 Size: 57 26 4 102 157 889 1629 43 Fragments arranged by size: 1629 1425 889 408 275 265 197 157 102 57 54 50 43 26 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2783 3046 1537 Size: 275 971 263 4090 Fragments arranged by size: 4090 971 275 263 EarI CTCTTCn'nnn_ Cuts at: 1353 2622 2714 3529 1353 Size: 1269 92 815 3423 Fragments arranged by size: 3423 1269 815 92 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2668 4186 4560 4706 387 Size: 1064 138 1079 1518 374 146 1280 Fragments arranged by size: 1518 1280 1079 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2527 2707 2527 Size: 180 5419 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2292 2458 3662 4259 5046 5314 Size: 393 612 48 166 1204 597 787 268 Cuts at: 5314 1239 Size: 1524 Fragments arranged by size: 1524 1204 787 612 597 393 268 166 48 EcoO109I rG'GnC_Cy Cuts at: 2323 2361 2323 Size: 38 5561 EcoRI G'AATT_C Cuts at: 2908 3286 2908 Size: 378 5221 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2305 2614 2305 Size: 309 5290 FseI GG_CCGG'CC Cuts at: 2789 2789 Size: 5599 FspI TGC'GCA Cuts at: 4105 4105 Size: 5599 HaeII r_GCGC'y Cuts at: 1678 4746 1678 Size: 3068 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 4240 4272 4314 Size: 32 135 32 614 32 2022 32 42 Cuts at: 4314 4346 1373 Size: 32 2626 Fragments arranged by size: 2626 2022 614 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2943 3189 669 Size: 988 281 1005 246 3079 Fragments arranged by size: 3079 1005 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5599 HpaI GTT'AAC Cuts at: 3189 3189 Size: 5599 KpnI G_GTAC'C Cuts at: 2924 2924 Size: 5599 MfeI C'AATT_G Cuts at: 3198 3198 Size: 5599 MluI A'CGCG_T Cuts at: 5475 5475 Size: 5599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4713 4897 1305 Size: 3408 184 2007 Fragments arranged by size: 3408 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2294 10 Size: 54 1425 343 462 3315 Fragments arranged by size: 3315 1425 462 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2393 2579 3557 3916 4075 518 Size: 648 1227 186 978 359 159 2042 Fragments arranged by size: 2042 1227 978 648 359 186 159 MspA1I CmG'CkG Cuts at: 1606 2338 2539 2607 3015 3692 4840 5085 1606 Size: 732 201 68 408 677 1148 245 2120 Fragments arranged by size: 2120 1148 732 677 408 245 201 68 NaeI GCC'GGC Cuts at: 2787 2787 Size: 5599 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5047 NdeI CA'TA_TG Cuts at: 387 387 Size: 5599 NgoMIV G'CCGG_C Cuts at: 2785 2785 Size: 5599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5599 NotI GC'GGCC_GC Cuts at: 3046 3046 Size: 5599 NspI r_CATG'y Cuts at: 1132 1229 2957 4502 1132 Size: 97 1728 1545 2229 Fragments arranged by size: 2229 1728 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5599 PciI A'CATG_T Cuts at: 4498 4498 Size: 5599 PflMI CCAn_nnn'nTGG Cuts at: 1108 2887 1108 Size: 1779 3820 PfoI T'CCnGG_A Cuts at: 2269 2269 Size: 5599 PmeI GTTT'AAAC Cuts at: 2903 2903 Size: 5599 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3668 3700 5181 5213 3668 Size: 32 1481 32 4054 Fragments arranged by size: 4054 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2323 2361 2323 Size: 38 5561 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5599 PsiI TTA'TAA Cuts at: 3169 3169 Size: 5599 PstI C_TGCA'G Cuts at: 830 2460 2691 2951 830 Size: 1630 231 260 3478 Fragments arranged by size: 3478 1630 260 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5599 PvuII CAG'CTG Cuts at: 2338 2539 2338 Size: 201 5398 SacI G_AGCT'C Cuts at: 2529 2709 2529 Size: 180 5419 SalI G'TCGA_C Cuts at: 1936 2941 1936 Size: 1005 4594 SapI GCTCTTCn'nnn_ Cuts at: 2714 2714 Size: 5599 SbfI CC_TGCA'GG Cuts at: 2460 2951 2460 Size: 491 5108 ScaI AGT'ACT Cuts at: 3847 5559 3847 Size: 1712 3887 SfcI C'TryA_G Cuts at: 826 1045 2456 2687 2947 4082 4763 4954 826 Size: 219 1411 231 260 1135 681 191 1471 Fragments arranged by size: 1471 1411 1135 681 260 231 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 SgrAI Cr'CCGG_yG Cuts at: 1700 2046 1700 Size: 346 5253 SmaI CCC'GGG Cuts at: 1872 2926 1872 Size: 1054 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2858 3703 4604 4866 820 Size: 197 926 915 845 901 262 1553 Fragments arranged by size: 1553 926 915 901 845 262 197 SnaBI TAC'GTA Cuts at: 493 3390 493 Size: 2897 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5599 SphI G_CATG'C Cuts at: 1132 2957 1132 Size: 1825 3774 SspI AAT'ATT Cuts at: 5 52 3523 5 Size: 47 3471 2081 Fragments arranged by size: 3471 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5599 StyI C'CwwG_G Cuts at: 513 1065 2760 3038 513 Size: 552 1695 278 3074 Fragments arranged by size: 3074 1695 552 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2186 2514 3626 3643 3796 3981 4426 Size: 1255 606 328 1112 17 153 185 445 Cuts at: 4426 325 Size: 1498 Fragments arranged by size: 1498 1255 1112 606 445 328 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3845 5557 96 Size: 274 80 33 51 1443 1868 1712 138 Fragments arranged by size: 1868 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5559 5591 5559 Size: 32 5567 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5599 XbaI T'CTAG_A Cuts at: 2935 2935 Size: 5599 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2578 2578 Size: 5599 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5599 XmaI C'CCGG_G Cuts at: 1870 2924 1870 Size: 1054 4545 XmnI GAAnn'nnTTC Cuts at: 3728 3728 Size: 5599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3407 276 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI Bsu36I EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI