(Circular) (Six-base) MAPSORT of: pFN21AB6565.seq Check: 137 from: 1 to: 5827 pFN21AB6565 5827 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2695 2695 Size: 5827 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3637 278 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3170 1937 Size: 1233 4594 Acc65I G'GTAC_C Cuts at: 2394 3148 2394 Size: 754 5073 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 2624 2786 3890 5274 5542 1632 Size: 872 120 162 1104 1384 268 1917 Fragments arranged by size: 1917 1384 1104 872 268 162 120 AfeI AGC'GCT Cuts at: 2822 2822 Size: 5827 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 4726 5703 1203 Size: 3523 977 1327 Fragments arranged by size: 3523 1327 977 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5827 AlwNI CAG_nnn'CTG Cuts at: 3570 5142 3570 Size: 1572 4255 ApaI G_GGCC'C Cuts at: 2796 2796 Size: 5827 ApaLI G'TGCA_C Cuts at: 3884 5040 3884 Size: 1156 4671 ApoI r'AATT_y Cuts at: 1493 3136 3362 3514 5623 1493 Size: 1643 226 152 2109 1697 Fragments arranged by size: 2109 1697 1643 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 2137 2883 3152 1870 Size: 73 194 746 269 4545 Fragments arranged by size: 4545 746 269 194 73 BamHI G'GATC_C Cuts at: 2086 2328 3157 3524 2086 Size: 242 829 367 4389 Fragments arranged by size: 4389 829 367 242 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2394 2740 3148 4603 Size: 325 217 627 291 316 346 408 1455 Cuts at: 4603 618 Size: 1842 Fragments arranged by size: 1842 1455 627 408 346 325 316 291 217 BanII G_rGCy'C Cuts at: 1391 2382 2517 2796 3146 1391 Size: 991 135 279 350 4072 Fragments arranged by size: 4072 991 350 279 135 BbeI G_GCGC'C Cuts at: 2082 2082 Size: 5827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2110 2792 2887 928 Size: 971 211 682 95 3868 Fragments arranged by size: 3868 971 682 211 95 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2965 2999 4018 4052 1903 Size: 34 1028 34 1019 34 3678 Fragments arranged by size: 3678 1028 1019 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4438 136 Size: 107 122 71 631 605 2766 1525 Fragments arranged by size: 2766 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2734 5822 2734 Size: 3088 2739 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 2796 3888 5044 1790 Size: 1006 1092 1156 2573 Fragments arranged by size: 2573 1156 1092 1006 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5827 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2104 3558 4516 Size: 416 53 83 399 24 687 1454 958 Cuts at: 4516 442 Size: 1753 Fragments arranged by size: 1753 1454 958 687 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5827 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 5827 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2737 4487 1239 Size: 815 683 1750 2579 Fragments arranged by size: 2579 1750 815 683 Bpu10I CC'TnA_GC Cuts at: 2129 2129 Size: 5827 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3952 4817 5115 3952 Size: 865 298 4664 Fragments arranged by size: 4664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4490 882 Size: 617 2991 2219 Fragments arranged by size: 2991 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3618 493 Size: 711 2414 2702 Fragments arranged by size: 2702 2414 711 BsaBI GATnn'nnATC Cuts at: 2531 5821 2531 Size: 3290 2537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 2115 2193 2241 Size: 53 83 186 936 546 36 78 48 Cuts at: 2241 2874 3634 4016 4651 275 Size: 633 760 382 635 1451 Fragments arranged by size: 1451 936 760 635 633 546 382 186 83 78 53 48 36 BsaWI w'CCGG_w Cuts at: 2148 2814 4260 4658 4932 5079 5490 2148 Size: 666 1446 398 274 147 411 2485 Fragments arranged by size: 2485 1446 666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2084 2114 1178 Size: 30 165 30 681 30 4891 Fragments arranged by size: 4891 681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 1186 Size: 1030 4797 BseYI C'CCAG_C Cuts at: 5030 5526 5030 Size: 496 5331 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5254 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2051 2998 3277 4038 5066 1540 Size: 275 186 50 947 279 761 1028 2301 Fragments arranged by size: 2301 1028 947 761 279 275 186 50 BsiHKAI G_wGCw'C Cuts at: 2482 2541 3888 3973 5044 2482 Size: 59 1347 85 1071 3265 Fragments arranged by size: 3265 1347 1071 85 59 BsmI GAATG_Cn' Cuts at: 2711 3338 3431 2711 Size: 627 93 5107 Fragments arranged by size: 5107 627 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3014 3014 Size: 5827 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 2482 2517 2541 2796 3146 3888 Size: 399 592 100 35 24 255 350 742 Cuts at: 3888 3973 5044 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 592 399 350 255 100 85 35 24 BspEI T'CCGG_A Cuts at: 2814 5490 2814 Size: 2676 3151 BspHI T'CATG_A Cuts at: 3716 5446 3716 Size: 1730 4097 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2581 2695 2709 3182 844 Size: 1737 114 14 473 3489 Fragments arranged by size: 3489 1737 473 114 14 BsrBI CCG'CTC Cuts at: 1394 1823 2017 3714 1394 Size: 429 194 1697 3507 Fragments arranged by size: 3507 1697 429 194 BsrDI GCAATG_nn' Cuts at: 65 1248 4322 4496 65 Size: 1183 3074 174 1396 Fragments arranged by size: 3074 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2286 2560 4471 4658 1700 Size: 110 476 274 1911 187 2869 Fragments arranged by size: 2869 1911 476 274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5827 BssHII G'CGCG_C Cuts at: 1925 2060 1925 Size: 135 5692 BssSI C'ACGA_G Cuts at: 3887 4899 3887 Size: 1012 4815 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5827 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 2593 1184 Size: 1409 4418 BstYI r'GATC_y Cuts at: 1972 2086 2328 2734 3157 3524 3909 3926 5378 Size: 114 242 406 423 367 385 17 1452 Cuts at: 5378 5464 5476 5822 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 423 406 385 367 346 242 114 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2230 2581 2832 513 Size: 552 939 226 351 251 3508 Fragments arranged by size: 3508 939 552 351 251 226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 2330 3105 3343 4137 4157 2330 Size: 775 238 794 20 4000 Fragments arranged by size: 4000 794 775 238 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2896 2931 529 Size: 35 2332 35 3425 Fragments arranged by size: 3425 2332 35 35 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2048 2422 Size: 54 1425 50 275 18 177 41 374 Cuts at: 2422 2562 2572 2966 3274 4163 5792 8 Size: 140 10 394 308 889 1629 43 Fragments arranged by size: 1629 1425 889 394 374 308 275 177 140 54 50 43 41 18 10 EagI C'GGCC_G Cuts at: 1537 1812 2048 3274 1537 Size: 275 236 1226 4090 Fragments arranged by size: 4090 1226 275 236 EarI CTCTTCn'nnn_ Cuts at: 1353 3757 1353 Size: 2404 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 4414 4788 4934 387 Size: 1064 138 585 2240 374 146 1280 Fragments arranged by size: 2240 1280 1064 585 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2504 2624 2737 2786 3890 4487 Size: 393 422 450 120 113 49 1104 597 Cuts at: 4487 5274 5542 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1104 787 597 450 422 393 268 120 113 49 EcoO109I rG'GnC_Cy Cuts at: 2792 2792 Size: 5827 EcoRI G'AATT_C Cuts at: 3136 3514 3136 Size: 378 5449 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 2082 2824 4974 1678 Size: 404 742 2150 2531 Fragments arranged by size: 2531 2150 742 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2877 2909 3011 Size: 32 135 32 512 32 761 32 102 Cuts at: 3011 3043 4468 4500 4542 4574 1373 Size: 32 1425 32 42 32 2626 Fragments arranged by size: 2626 1425 761 512 135 102 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3171 3417 669 Size: 988 281 1233 246 3079 Fragments arranged by size: 3079 1233 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KasI G'GCGC_C Cuts at: 2078 2078 Size: 5827 KpnI G_GTAC'C Cuts at: 2398 3152 2398 Size: 754 5073 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2325 3054 4941 5125 1305 Size: 1020 729 1887 184 2007 Fragments arranged by size: 2007 1887 1020 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2968 10 Size: 54 1425 343 177 959 2869 Fragments arranged by size: 2869 1425 959 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3785 4144 4303 518 Size: 648 2619 359 159 2042 Fragments arranged by size: 2619 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 3243 3920 5068 5313 1606 Size: 441 1196 677 1148 245 2120 Fragments arranged by size: 2120 1196 1148 677 441 245 NaeI GCC'GGC Cuts at: 2562 2562 Size: 5827 NarI GG'CG_CC Cuts at: 2079 2079 Size: 5827 NcoI C'CATG_G Cuts at: 513 1065 2004 2832 513 Size: 552 939 828 3508 Fragments arranged by size: 3508 939 828 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NgoMIV G'CCGG_C Cuts at: 2560 2560 Size: 5827 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5827 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 3185 4730 1132 Size: 97 1956 1545 2229 Fragments arranged by size: 2229 1956 1545 97 PasI CC'CwG_GG Cuts at: 1348 2717 2953 1348 Size: 1369 236 4222 Fragments arranged by size: 4222 1369 236 PciI A'CATG_T Cuts at: 4726 4726 Size: 5827 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5827 PfoI T'CCnGG_A Cuts at: 2031 2753 2031 Size: 722 5105 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5827 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PspOMI G'GGCC_C Cuts at: 2792 2792 Size: 5827 PstI C_TGCA'G Cuts at: 830 2046 2592 2706 3179 830 Size: 1216 546 114 473 3478 Fragments arranged by size: 3478 1216 546 473 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SbfI CC_TGCA'GG Cuts at: 2592 2706 3179 2592 Size: 114 473 5240 Fragments arranged by size: 5240 473 114 ScaI AGT'ACT Cuts at: 4075 5787 4075 Size: 1712 4115 SfcI C'TryA_G Cuts at: 826 1045 2042 2588 2702 3175 4310 4991 5182 Size: 219 997 546 114 473 1135 681 191 Cuts at: 5182 826 Size: 1471 Fragments arranged by size: 1471 1135 997 681 546 473 219 191 114 SfoI GGC'GCC Cuts at: 2080 2080 Size: 5827 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 3154 1872 Size: 1282 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3931 4832 5094 820 Size: 197 926 1988 901 262 1553 Fragments arranged by size: 1988 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 3185 1132 Size: 2053 3774 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5827 StyI C'CwwG_G Cuts at: 513 1065 2004 2010 2508 2517 2832 3266 513 Size: 552 939 6 498 9 315 434 3074 Fragments arranged by size: 3074 939 552 498 434 315 9 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2966 3034 3854 3871 4024 4209 4654 Size: 1255 1386 68 820 17 153 185 445 Cuts at: 4654 325 Size: 1498 Fragments arranged by size: 1498 1386 1255 820 445 185 153 68 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2853 4073 5785 Size: 274 80 33 51 1443 876 1220 1712 Cuts at: 5785 96 Size: 138 Fragments arranged by size: 1712 1443 1220 876 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5787 5819 5787 Size: 32 5795 Tth111I GACn'n_nGTC Cuts at: 1316 3025 1316 Size: 1709 4118 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2969 2969 Size: 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 3152 1870 Size: 1282 4545 XmnI GAAnn'nnTTC Cuts at: 2489 3956 2489 Size: 1467 4360 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3635 276 Size: 53 83 186 936 2101 2468 Fragments arranged by size: 2468 2101 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI