Restriction map for
pFN21AB6338
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoT14I EcoT22I
FauI FbaI FokI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MscI
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StyI
TfiI Tth111I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 725 GGTACC G^GTACC
AccI 349 GTCGAC GT^[AC][GT]AC
AccIII 68 TCCGGA T^CCGGA
AcsI 838 AAATTT [GA]^AATT[CT]
AlwNI 656 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 838 AAATTT [GA]^AATT[CT]
Asp718I 725 GGTACC G^GTACC
AspHI 217 GTGCTC G[AT]GC[AT]^C
BanII 64 GGGCTC G[GA]GC[CT]^C
BcnI 402 CCCGG CC^[GC]GG
BglII 171 AGATCT A^GATCT
BsaWI 68 TCCGGA [AT]^CCGG[AT]
BseAI 68 TCCGGA T^CCGGA
BseRI 121 CTCCTC GAGGAG(10/8)
BsgI 406 GTGCAG GTGCAG(16/14)
BsiHKAI 217 GTGCTC G[AT]GC[AT]^C
BsmFI 55 GTCCC GGGAC(10/14)
BspEI 68 TCCGGA T^CCGGA
BstBI 806 TTCGAA TT^CGAA
BstYI 171 AGATCT [GA]^GATC[CT]
DpnII 172 GATC ^GATC
DraI 841 TTTAAA TTT^AAA
DraIII 279 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 72 GATATC GAT^ATC
HgaI 337 GACGC GACGC(5/10)
HinfI 237 GACTC G^A[ACGT]TC
KpnI 725 GGTACC GGTAC^C
MboI 172 GATC ^GATC
MflI 171 AGATCT [GA]^GATC[CT]
MroI 68 TCCGGA T^CCGGA
MseI 842 TTAA T^TAA
MslI 92 CACTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 402 CCCGG CC^[GC]GG
NdeII 172 GATC ^GATC
NspI 158 GCATGC [GA]CATG^[CT]
NspV 806 TTCGAA TT^CGAA
PflMI 278 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 237 GACTC GAGTC(4/5)
PpuMI 854 AGGACCC [GA]G^G[AT]CC[CT]
PstI 621 CTGCAG CTGCA^G
SalI 349 GTCGAC G^TCGAC
Sau3AI 172 GATC ^GATC
SfaNI 104 GCATC GCATC(5/9)
SfcI 621 CTGCAG C^T[GA][CT]AG
SfuI 806 TTCGAA TT^CGAA
SphI 158 GCATGC GCATG^C
StuI 317 AGGCCT AGG^CCT
SwaI 840 ATTTAAAT ATTT^AAAT
Tru9I 842 TTAA T^TAA
Van91I 278 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 171 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 162 GCGG CCGC(-3/-1)
608 GCGG
AocI 265 CCTGAGG CC^T[ACGT]AGG
483 CCTGAGG
AvaI 220 CTCGAG C^[CT]CG[GA]G
321 CTCGAG
BanI 371 GGTGCC G^G[CT][GA]CC
725 GGTACC
BmyI 64 GGGCTC G[AGT]GC[ACT]^C
217 GTGCTC
BsoBI 220 CTCGAG C^[CT]CG[GA]G
321 CTCGAG
Bsp1286I 64 GGGCTC G[AGT]GC[ACT]^C
217 GTGCTC
BsrI 824 ACTGG ACTGG(1/-1)
96 CCAGT
Bsu36I 265 CCTGAGG CC^T[ACGT]AGG
483 CCTGAGG
DraII 443 AGGCCCC [GA]G^G[ACGT]CC[CT]
854 AGGACCC
Eco81I 265 CCTGAGG CC^T[ACGT]AGG
483 CCTGAGG
EcoO109I 443 AGGCCCC [GA]G^G[ACGT]CC[CT]
854 AGGACCC
HaeIII 318 GGCC GG^CC
444 GGCC
HapII 69 CCGG C^CGG
403 CCGG
HincII 349 GTCGAC GT[CT]^[GA]AC
696 GTCAAC
HindII 349 GTCGAC GT[CT]^[GA]AC
696 GTCAAC
HpaII 69 CCGG C^CGG
403 CCGG
MspI 69 CCGG C^CGG
403 CCGG
NlaIII 108 CATG CATG^
159 CATG
PaeR7I 220 CTCGAG C^TCGAG
321 CTCGAG
XhoI 220 CTCGAG C^TCGAG
321 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AfaI AluI AvaII BbsI BbvI
BfaI BpmI BpuAI BsaJI BsiYI
BslI BsmAI BssKI BstNI Cac8I
Cfr13I DdeI EcoRII Fnu4HI HphI
ItaI MaeI MaeIII MboII MnlI
MspA1I MvaI NlaIV PvuII RsaI
Sau96I ScrFI SmlI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I