(Circular) (Six-base) MAPSORT of: pFN21AB6338.seq Check: 5762 from: 1 to: 5575 pFN21AB6338 5575 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3385 278 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2355 2918 1937 Size: 418 563 4594 Fragments arranged by size: 4594 563 418 Acc65I G'GTAC_C Cuts at: 2730 2896 2730 Size: 166 5409 AclI AA'CG_TT Cuts at: 1563 3702 4075 1563 Size: 2139 373 3063 Fragments arranged by size: 3063 2139 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2193 2267 2643 3638 5022 5290 1632 Size: 561 74 376 995 1384 268 1917 Fragments arranged by size: 1917 1384 995 561 376 268 74 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5378 AflIII A'CryG_T Cuts at: 1203 4474 5451 1203 Size: 3271 977 1327 Fragments arranged by size: 3271 1327 977 AgeI A'CCGG_T Cuts at: 4406 4406 Size: 5575 AhdI GACnn_n'nnGTC Cuts at: 4304 4304 Size: 5575 AleI CACnn'nnGTG Cuts at: 1166 2101 1166 Size: 935 4640 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5575 AlwNI CAG_nnn'CTG Cuts at: 2666 3318 4890 2666 Size: 652 1572 3351 Fragments arranged by size: 3351 1572 652 ApaLI G'TGCA_C Cuts at: 3632 4788 3632 Size: 1156 4419 ApoI r'AATT_y Cuts at: 1493 2843 2884 3110 3262 5371 1493 Size: 1350 41 226 152 2109 1697 Fragments arranged by size: 2109 1697 1350 226 152 41 AseI AT'TA_AT Cuts at: 160 1032 4129 160 Size: 872 3097 1606 Fragments arranged by size: 3097 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 AvaI C'yCGr_G Cuts at: 1870 1943 2225 2326 2900 1870 Size: 73 282 101 574 4545 Fragments arranged by size: 4545 574 282 101 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2714 2747 2714 Size: 33 5542 BamHI G'GATC_C Cuts at: 2905 3272 2905 Size: 367 5208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2376 2730 2896 4351 618 Size: 325 217 627 589 354 166 1455 1842 Fragments arranged by size: 1842 1455 627 589 354 325 217 166 BanII G_rGCy'C Cuts at: 1391 2073 2894 1391 Size: 682 821 4072 Fragments arranged by size: 4072 821 682 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2481 2649 2807 928 Size: 971 582 168 158 3696 Fragments arranged by size: 3696 971 582 168 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3766 3800 1903 Size: 34 1829 34 3678 Fragments arranged by size: 3678 1829 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4677 3467 Size: 1210 4365 BclI T'GATC_A Cuts at: 1548 1548 Size: 5575 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4186 136 Size: 107 122 71 631 605 2514 1525 Fragments arranged by size: 2514 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2176 5570 2176 Size: 3394 2181 BlpI GC'TnA_GC Cuts at: 2992 2992 Size: 5575 Bme1580I G_kGCm'C Cuts at: 1790 3636 4792 1790 Size: 1846 1156 2573 Fragments arranged by size: 2573 1846 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5575 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3306 4264 442 Size: 416 53 83 399 24 1889 958 1753 Fragments arranged by size: 1889 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5575 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2222 2429 4235 1239 Size: 845 138 207 1806 2579 Fragments arranged by size: 2579 1806 845 207 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2299 2670 2850 3700 4565 4863 2299 Size: 371 180 850 865 298 3011 Fragments arranged by size: 3011 865 850 371 298 180 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4238 882 Size: 617 2739 2219 Fragments arranged by size: 2739 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3366 493 Size: 711 2162 2702 Fragments arranged by size: 2702 2162 711 BsaBI GATnn'nnATC Cuts at: 5569 5569 Size: 5575 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3382 3764 4399 275 Size: 53 83 186 936 1849 382 635 1451 Fragments arranged by size: 1849 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2073 4008 4406 4680 4827 5238 2073 Size: 1935 398 274 147 411 2410 Fragments arranged by size: 2410 1935 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2600 2630 1178 Size: 30 165 30 1197 30 4123 Fragments arranged by size: 4123 1197 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 1186 Size: 931 4644 BseYI C'CCAG_C Cuts at: 2018 4778 5274 2018 Size: 2760 496 2319 Fragments arranged by size: 2760 2319 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2432 1163 Size: 573 696 4306 Fragments arranged by size: 4306 696 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3025 3786 4814 1540 Size: 275 186 1024 761 1028 2301 Fragments arranged by size: 2301 1028 1024 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 3636 3721 4792 2226 Size: 1410 85 1071 3009 Fragments arranged by size: 3009 1410 1071 85 BsmI GAATG_Cn' Cuts at: 3086 3179 3086 Size: 93 5482 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2226 2894 3636 3721 4792 1391 Size: 399 283 153 668 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 668 399 283 153 85 BspEI T'CCGG_A Cuts at: 2073 5238 2073 Size: 3165 2410 BspHI T'CATG_A Cuts at: 3464 5194 3464 Size: 1730 3845 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 844 Size: 2086 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3462 1394 Size: 429 1639 3507 Fragments arranged by size: 3507 1639 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4070 4244 65 Size: 1183 2822 174 1396 Fragments arranged by size: 2822 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4219 4406 1700 Size: 110 2409 187 2869 Fragments arranged by size: 2869 2409 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5575 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5575 BssSI C'ACGA_G Cuts at: 3635 4647 3635 Size: 1012 4563 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5575 BstBI TT'CG_AA Cuts at: 2812 4379 2812 Size: 1567 4008 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5575 BstYI r'GATC_y Cuts at: 1972 2176 2905 3272 3657 3674 5126 5212 5224 Size: 204 729 367 385 17 1452 86 12 Cuts at: 5224 5570 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 729 385 367 346 204 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2271 2489 2271 Size: 218 5357 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4084 Fragments arranged by size: 4084 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4836 Fragments arranged by size: 4836 683 56 BtsI GCAGTG_nn' Cuts at: 2511 2703 2778 3091 3885 3905 2511 Size: 192 75 313 794 20 4181 Fragments arranged by size: 4181 794 313 192 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5575 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2172 2207 529 Size: 35 1608 35 3897 Fragments arranged by size: 3897 1608 35 35 DraI TTT'AAA Cuts at: 2848 2879 2848 Size: 31 5544 DraIII CAC_nnn'GTG Cuts at: 2289 2289 Size: 5575 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4582 809 Size: 507 3266 1802 Fragments arranged by size: 3266 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3022 3911 5540 Size: 54 1425 50 275 18 1192 889 1629 Cuts at: 5540 8 Size: 43 Fragments arranged by size: 1629 1425 1192 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3022 1537 Size: 275 1210 4090 Fragments arranged by size: 4090 1210 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3505 1353 Size: 2152 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4162 4536 4682 387 Size: 1064 138 2573 374 146 1280 Fragments arranged by size: 2573 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2193 2222 2267 2429 2643 3638 Size: 393 452 109 29 45 162 214 995 Cuts at: 3638 4235 5022 5290 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 995 787 597 452 393 268 214 162 109 45 29 EcoO109I rG'GnC_Cy Cuts at: 2449 2860 2449 Size: 411 5164 EcoRI G'AATT_C Cuts at: 2884 3262 2884 Size: 378 5197 EcoRV GAT'ATC Cuts at: 2079 2079 Size: 5575 FspI TGC'GCA Cuts at: 4081 4081 Size: 5575 HaeII r_GCGC'y Cuts at: 1678 4722 1678 Size: 3044 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2161 2193 2600 2632 4216 Size: 32 135 32 589 32 407 32 1584 Cuts at: 4216 4248 4290 4322 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1584 589 407 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2356 2703 2919 3165 669 Size: 988 281 418 347 216 246 3079 Fragments arranged by size: 3079 988 418 347 281 246 216 HindIII A'AGCT_T Cuts at: 748 748 Size: 5575 HpaI GTT'AAC Cuts at: 3165 3165 Size: 5575 KpnI G_GTAC'C Cuts at: 2734 2900 2734 Size: 166 5409 MfeI C'AATT_G Cuts at: 3174 3174 Size: 5575 MluI A'CGCG_T Cuts at: 5451 5451 Size: 5575 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4689 4873 1305 Size: 3384 184 2007 Fragments arranged by size: 3384 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3753 Fragments arranged by size: 3753 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2101 3533 3892 4051 518 Size: 648 935 1432 359 159 2042 Fragments arranged by size: 2042 1432 935 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2159 2502 2613 2991 3668 4816 5061 Size: 516 37 343 111 378 677 1148 245 Cuts at: 5061 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 516 378 343 245 111 37 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4084 Fragments arranged by size: 4084 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5575 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5575 NotI GC'GGCC_GC Cuts at: 3022 3022 Size: 5575 NspI r_CATG'y Cuts at: 1132 1229 2167 2933 4478 1132 Size: 97 938 766 1545 2229 Fragments arranged by size: 2229 1545 938 766 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5575 PciI A'CATG_T Cuts at: 4474 4474 Size: 5575 PflMI CCAn_nnn'nTGG Cuts at: 1108 2289 1108 Size: 1181 4394 PfoI T'CCnGG_A Cuts at: 2199 2199 Size: 5575 PmeI GTTT'AAAC Cuts at: 2879 2879 Size: 5575 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3644 3676 5157 5189 3644 Size: 32 1481 32 4030 Fragments arranged by size: 4030 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2860 2860 Size: 5575 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5575 PsiI TTA'TAA Cuts at: 3145 3145 Size: 5575 PspXI vC'TCGA_Gb Cuts at: 2326 2326 Size: 5575 PstI C_TGCA'G Cuts at: 830 2630 2927 830 Size: 1800 297 3478 Fragments arranged by size: 3478 1800 297 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5575 PvuII CAG'CTG Cuts at: 2122 2159 2502 2122 Size: 37 343 5195 Fragments arranged by size: 5195 343 37 SalI G'TCGA_C Cuts at: 1936 2354 2917 1936 Size: 418 563 4594 Fragments arranged by size: 4594 563 418 SbfI CC_TGCA'GG Cuts at: 2927 2927 Size: 5575 ScaI AGT'ACT Cuts at: 3823 5535 3823 Size: 1712 3863 SfcI C'TryA_G Cuts at: 826 1045 2626 2923 4058 4739 4930 826 Size: 219 1581 297 1135 681 191 1471 Fragments arranged by size: 1581 1471 1135 681 297 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5575 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5575 SmaI CCC'GGG Cuts at: 1872 2902 1872 Size: 1030 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2225 2314 2326 2685 2865 3679 Size: 197 926 282 89 12 359 180 814 Cuts at: 3679 4580 4842 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 814 359 282 262 197 180 89 12 SnaBI TAC'GTA Cuts at: 493 3366 493 Size: 2873 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5575 SphI G_CATG'C Cuts at: 1132 2167 2933 1132 Size: 1035 766 3774 Fragments arranged by size: 3774 1035 766 SspI AAT'ATT Cuts at: 5 52 3499 5 Size: 47 3447 2081 Fragments arranged by size: 3447 2081 47 StuI AGG'CCT Cuts at: 1517 2324 1517 Size: 807 4768 StyI C'CwwG_G Cuts at: 513 1065 2004 3014 513 Size: 552 939 1010 3074 Fragments arranged by size: 3074 1010 939 552 SwaI ATTT'AAAT Cuts at: 2848 2848 Size: 5575 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2449 3602 3619 3772 3957 4402 Size: 1255 417 452 1153 17 153 185 445 Cuts at: 4402 325 Size: 1498 Fragments arranged by size: 1498 1255 1153 452 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2370 3821 5533 Size: 274 80 33 51 1443 393 1451 1712 Cuts at: 5533 96 Size: 138 Fragments arranged by size: 1712 1451 1443 393 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5535 5567 5535 Size: 32 5543 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5575 XbaI T'CTAG_A Cuts at: 2911 2911 Size: 5575 XhoI C'TCGA_G Cuts at: 1943 2225 2326 1943 Size: 282 101 5192 Fragments arranged by size: 5192 282 101 XmaI C'CCGG_G Cuts at: 1870 2900 1870 Size: 1030 4545 XmnI GAAnn'nnTTC Cuts at: 3704 3704 Size: 5575 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3383 276 Size: 53 83 186 936 1849 2468 Fragments arranged by size: 2468 1849 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI