Restriction map for
pFN21AB8146
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspHI
AspI AviII AvrII BanII BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
Cfr10I ClaI DraI EagI EclXI
Eco52I Eco81I EcoNI EcoRI FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeII MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 830 CGCG CG^CG
AfaI 14 GTAC GT^AC
AhdI 1054 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 478 AGCGCT AGC^GCT
AspEI 1054 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 122 CCCGGG C^[CT]CG[GA]G
BalI 863 TGGCCA TGG^CCA
BamHI 156 GGATCC G^GATCC
BanI 146 GGCACC G^G[CT][GA]CC
BcgI 318 CGAAGGTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 193 CTAG C^TAG
BlpI 697 GCTGAGC GC^T[ACGT]AGC
BmyI 145 GGGCAC G[AGT]GC[ACT]^C
BpmI 187 CTGGAG CTGGAG(16/14)
Bpu1102I 697 GCTGAGC GC^T[ACGT]AGC
BsgI 504 CTGCAC GTGCAG(16/14)
BsmI 567 GAATGC GAATGC(1/-1)
BsoBI 122 CCCGGG C^[CT]CG[GA]G
Bsp1286I 145 GGGCAC G[AGT]GC[ACT]^C
BspMI 84 ACCTGC ACCTGC(4/8)
BsrDI 350 GCAATG GCAATG(2/0)
BstEII 846 GGTAACC G^GT[ACGT]ACC
BstPI 846 GGTAACC G^GT[ACGT]ACC
BstUI 830 CGCG CG^CG
CelII 697 GCTGAGC GC^T[ACGT]AGC
CpoI 415 CGGACCG CG^G[AT]CCG
DraII 1040 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 987 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 680 GACTGAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 863 TGGCCA [CT]^GGCC[GA]
Eam1105I 1054 GACTACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 478 AGCGCT AGC^GCT
EcoO109I 1040 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 846 GGTAACC G^GT[ACGT]ACC
EcoRV 658 GATATC GAT^ATC
EcoT22I 569 ATGCAT ATGCA^T
MaeI 193 CTAG C^TAG
MluNI 863 TGGCCA TGG^CCA
MscI 863 TGGCCA TGG^CCA
MvnI 830 CGCG CG^CG
NcoI 842 CCATGG C^CATGG
NheI 192 GCTAGC G^CTAGC
NsiI 569 ATGCAT ATGCA^T
PflMI 64 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1040 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 14 GTAC GT^AC
RsrII 415 CGGACCG CG^G[AT]CCG
SmaI 122 CCCGGG CCC^GGG
SmlI 178 CTCAAG C^T[CT][GA]AG
Van91I 64 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 575 CCAACAGCGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 122 CCCGGG C^CCGGG
XmaI 122 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 741 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1083 GAAGCCCTTC
BseRI 93 CTCCTC GAGGAG(10/8)
718 GAGGAG
BsmAI 374 GAGAC GTCTC(1/5)
647 GAGAC
BsmFI 936 GTCCC GGGAC(10/14)
1042 GTCCC
BstXI 370 CCAGGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
632 CCATCAATGTGG
BstYI 156 GGATCC [GA]^GATC[CT]
909 GGATCT
HaeII 132 GGCGCT [GA]GCGC^[CT]
478 AGCGCT
HphI 381 GGTGA GGTGA(8/7)
1112 TCACC
MflI 156 GGATCC [GA]^GATC[CT]
909 GGATCT
MseI 752 TTAA T^TAA
905 TTAA
MslI 258 CATAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
633 CATCAATGTG
MspA1I 579 CAGCGG C[AC]G^C[GT]G
1045 CCGCTG
NspI 702 GCATGT [GA]CATG^[CT]
758 ACATGC
PleI 284 GACTC GAGTC(4/5)
687 GAGTC
Tru9I 752 TTAA T^TAA
905 TTAA
XhoII 156 GGATCC [GA]^GATC[CT]
909 GGATCT
XmnI 741 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1083 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BbvI BcnI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeIII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I