Restriction map for
pFN21AB8364
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# NO CUT ENZYMES ( 114 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BanI
BbeI BciVI BclI BfaI BfrI
BlnI BsaBI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HgaI HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI TspRI
Tth111I Van91I XbaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 1435 GACGTC GACGT^C
AcsI 574 GAATTT [GA]^AATT[CT]
AcyI 1435 GACGTC G[GA]^CG[CT]C
AlwI 693 GATCC GGATC(4/5)
ApaI 82 GGGCCC GGGCC^C
ApoI 574 GAATTT [GA]^AATT[CT]
AspHI 1269 GAGCTC G[AT]GC[AT]^C
AvaII 240 GGACC G^G[AT]CC
AviII 260 TGCGCA TGC^GCA
BalI 1031 TGGCCA TGG^CCA
BbrPI 1403 CACGTG CAC^GTG
BcgI 801 CGAGGATATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 216 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 216 GCTGAGC GC^T[ACGT]AGC
BsaAI 1403 CACGTG [CT]AC^GT[GA]
BsaHI 1435 GACGTC G[GA]^CG[CT]C
BsaI 358 GAGACC GGTCTC(1/5)
BsaWI 932 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1269 GAGCTC G[AT]GC[AT]^C
BsmFI 708 GTCCC GGGAC(10/14)
BspLU11I 413 ACATGT A^CATGT
BssSI 75 CACGAG CACGAG(-5/-1)
BstEII 12 GGTGACC G^GT[ACGT]ACC
BstPI 12 GGTGACC G^GT[ACGT]ACC
CelII 216 GCTGAGC GC^T[ACGT]AGC
DrdI 15 GACCCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1031 TGGCCA [CT]^GGCC[GA]
EarI 685 GAAGAG CTCTTC(1/4)
EcoO65I 12 GGTGACC G^GT[ACGT]ACC
EcoT14I 740 CCATGG C^C[AT][AT]GG
FspI 260 TGCGCA TGC^GCA
Hin1I 1435 GACGTC G[GA]^CG[CT]C
HincII 202 GTCAAC GT[CT]^[GA]AC
HindII 202 GTCAAC GT[CT]^[GA]AC
Ksp632I 685 GAAGAG CTCTTC(1/4)
MluNI 1031 TGGCCA TGG^CCA
MscI 1031 TGGCCA TGG^CCA
MseI 1037 TTAA T^TAA
NcoI 740 CCATGG C^CATGG
PaeR7I 105 CTCGAG C^TCGAG
PmaCI 1403 CACGTG CAC^GTG
PmlI 1403 CACGTG CAC^GTG
SacI 1269 GAGCTC GAGCT^C
SfiI 967 GGCCTCAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 50 CCCGGG CCC^GGG
SmlI 105 CTCGAG C^T[CT][GA]AG
StyI 740 CCATGG C^C[AT][AT]GG
Tru9I 1037 TTAA T^TAA
Tsp45I 13 GTGAC ^GT[GC]AC
Tsp509I 575 AATT ^AATT
XcmI 1088 CCAAGCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 105 CTCGAG C^TCGAG
XmaCI 50 CCCGGG C^CCGGG
XmaI 50 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 413 ACATGT A^C[GA][CT]GT
1402 ACACGT
AocI 438 CCTTAGG CC^T[ACGT]AGG
669 CCTCAGG
BbsI 387 GAAGAC GAAGAC(2/6)
1239 GAAGAC
BglI 146 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
968 GCCTCAAAGGC
BglII 713 AGATCT A^GATCT
917 AGATCT
BpuAI 387 GAAGAC GAAGAC(2/6)
1239 GAAGAC
BspMI 896 ACCTGC ACCTGC(4/8)
1258 GCAGGT
BsrBI 66 CCGCTC CCGCTC(-3/-3)
753 CCGCTC
BsrDI 777 CATTGC GCAATG(2/0)
1002 CATTGC
BsrFI 944 GCCGGC [GA]^CCGG[CT]
1199 GCCGGC
BstXI 170 CCAGCGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1373 CCAGGGCCGTGG
Bsu36I 438 CCTTAGG CC^T[ACGT]AGG
669 CCTCAGG
Cfr10I 944 GCCGGC [GA]^CCGG[CT]
1199 GCCGGC
DraII 965 AGGGCCT [GA]G^G[ACGT]CC[CT]
1283 GGGGCCT
DsaI 740 CCATGG C^C[GA][CT]GG
1379 CCGTGG
Eco81I 438 CCTTAGG CC^T[ACGT]AGG
669 CCTCAGG
EcoO109I 965 AGGGCCT [GA]G^G[ACGT]CC[CT]
1283 GGGGCCT
HphI 12 GGTGA GGTGA(8/7)
188 TCACC
MaeIII 13 GTGAC ^GT[ACGT]AC
196 GTTAC
NaeI 944 GCCGGC GCC^GGC
1199 GCCGGC
NgoMIV 944 GCCGGC G^CCGGC
1199 GCCGGC
PvuII 448 CAGCTG CAG^CTG
1108 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaI BanII BbvCI BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI TthHB8I XhoII