Restriction map for
pFN21AB8364
(Linear) (Six-base) MAPSORT of: KIBB8364.seq Check: 174 from: 1 to: 1449
KIBB8364.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1249 1449
Size: 1249 200
AatII G_ACGT'C
Cuts at: 0 1439 1449
Size: 1439 10
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 496 1338 1449
Size: 42 454 842 111
Fragments arranged by size:
842 454 111 42
AflIII A'CryG_T
Cuts at: 0 413 1402 1449
Size: 413 989 47
Fragments arranged by size:
989 413 47
AleI CACnn'nnGTG
Cuts at: 0 727 1449
Size: 727 722
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 1449
Size: 400 32 1017
Fragments arranged by size:
1017 400 32
AlwNI CAG_nnn'CTG
Cuts at: 0 213 510 873 1128 1449
Size: 213 297 363 255 321
Fragments arranged by size:
363 321 297 255 213
ApaI G_GGCC'C
Cuts at: 0 86 1449
Size: 86 1363
ApoI r'AATT_y
Cuts at: 0 574 1449
Size: 574 875
AvaI C'yCGr_G
Cuts at: 0 50 105 116 1449
Size: 50 55 11 1333
Fragments arranged by size:
1333 55 50 11
BanII G_rGCy'C
Cuts at: 0 86 223 1273 1321 1345 1449
Size: 86 137 1050 48 24 104
Fragments arranged by size:
1050 137 104 86 48 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 394 1246 1449
Size: 394 852 203
Fragments arranged by size:
852 394 203
BbvCI CC'TCA_GC
Cuts at: 0 782 827 1303 1449
Size: 782 45 476 146
Fragments arranged by size:
782 476 146 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 790 824 1449
Size: 790 34 625
Fragments arranged by size:
790 625 34
BglI GCCn_nnn'nGGC
Cuts at: 0 152 974 1449
Size: 152 822 475
Fragments arranged by size:
822 475 152
BglII A'GATC_T
Cuts at: 0 713 917 1449
Size: 713 204 532
Fragments arranged by size:
713 532 204
BlpI GC'TnA_GC
Cuts at: 0 217 1449
Size: 217 1232
Bme1580I G_kGCm'C
Cuts at: 0 86 1449
Size: 86 1363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 355 376 472 502 649 934 1012
Size: 253 102 21 96 30 147 285 78
Cuts at: 1012 1087 1204 1225 1305 1329 1356 1449
Size: 75 117 21 80 24 27 93
Fragments arranged by size:
285 253 147 117 102 96 93 80
78 75 30 27 24 21 21
Bpu10I CC'TnA_GC
Cuts at: 0 782 827 1303 1449
Size: 782 45 476 146
Fragments arranged by size:
782 476 146 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 1449
Size: 352 1097
BsaAI yAC'GTr
Cuts at: 0 1405 1449
Size: 1405 44
BsaHI Gr'CG_yC
Cuts at: 0 1436 1449
Size: 1436 13
BsaWI w'CCGG_w
Cuts at: 0 932 1449
Size: 932 517
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 349 379 691 721 739 769 1449
Size: 349 30 312 30 18 30 680
Fragments arranged by size:
680 349 312 30 30 30 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 703 874 1207 1359 1449
Size: 93 610 171 333 152 90
Fragments arranged by size:
610 333 171 152 93 90
BseYI C'CCAG_C
Cuts at: 0 877 1449
Size: 877 572
BsiHKAI G_wGCw'C
Cuts at: 0 1273 1449
Size: 1273 176
Bsp1286I G_dGCh'C
Cuts at: 0 86 223 1273 1321 1345 1449
Size: 86 137 1050 48 24 104
Fragments arranged by size:
1050 137 104 86 48 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 905 1249 1449
Size: 905 344 200
Fragments arranged by size:
905 344 200
BsrBI CCG'CTC
Cuts at: 0 68 755 1449
Size: 68 687 694
Fragments arranged by size:
694 687 68
BsrDI GCAATG_nn'
Cuts at: 0 776 1001 1449
Size: 776 225 448
Fragments arranged by size:
776 448 225
BsrFI r'CCGG_y
Cuts at: 0 944 1199 1449
Size: 944 255 250
Fragments arranged by size:
944 255 250
BssSI C'ACGA_G
Cuts at: 0 75 1449
Size: 75 1374
BstEII G'GTnAC_C
Cuts at: 0 12 1449
Size: 12 1437
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 1380 1449
Size: 177 1203 69
Fragments arranged by size:
1203 177 69
BstYI r'GATC_y
Cuts at: 0 692 713 917 1449
Size: 692 21 204 532
Fragments arranged by size:
692 532 204 21
Bsu36I CC'TnA_GG
Cuts at: 0 439 670 1449
Size: 439 231 779
Fragments arranged by size:
779 439 231
BtgI C'CryG_G
Cuts at: 0 740 1379 1449
Size: 740 639 70
Fragments arranged by size:
740 639 70
DrdI GACnn_nn'nnGTC
Cuts at: 0 21 1449
Size: 21 1428
EaeI y'GGCC_r
Cuts at: 0 1031 1449
Size: 1031 418
EarI CTCTTCn'nnn_
Cuts at: 0 680 1449
Size: 680 769
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 502 1449
Size: 502 947
EcoICRI GAG'CTC
Cuts at: 0 1271 1449
Size: 1271 178
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 253 355 376 472 496 502 649
Size: 42 211 102 21 96 24 6 147
Cuts at: 649 934 1012 1087 1204 1225 1305 1329 1338
Size: 285 78 75 117 21 80 24 9
Cuts at: 1338 1356 1449
Size: 18 93
Fragments arranged by size:
285 211 147 117 102 96 93 80
78 75 42 24 24 21 21 18
9 6
EcoO109I rG'GnC_Cy
Cuts at: 0 966 1284 1449
Size: 966 318 165
Fragments arranged by size:
966 318 165
FspI TGC'GCA
Cuts at: 0 262 1449
Size: 262 1187
FspAI rTGC'GCAy
Cuts at: 0 262 1449
Size: 262 1187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 648 691 723 1449
Size: 616 32 43 32 726
Fragments arranged by size:
726 616 43 32 32
HincII GTy'rAC
Cuts at: 0 204 1449
Size: 204 1245
MscI TGG'CCA
Cuts at: 0 1033 1449
Size: 1033 416
MslI CAynn'nnrTG
Cuts at: 0 10 727 890 1449
Size: 10 717 163 559
Fragments arranged by size:
717 559 163 10
MspA1I CmG'CkG
Cuts at: 0 173 450 1083 1110 1250 1449
Size: 173 277 633 27 140 199
Fragments arranged by size:
633 277 199 173 140 27
NaeI GCC'GGC
Cuts at: 0 946 1201 1449
Size: 946 255 248
Fragments arranged by size:
946 255 248
NcoI C'CATG_G
Cuts at: 0 740 1449
Size: 740 709
NgoMIV G'CCGG_C
Cuts at: 0 944 1199 1449
Size: 944 255 250
Fragments arranged by size:
944 255 250
NspI r_CATG'y
Cuts at: 0 417 504 843 1449
Size: 417 87 339 606
Fragments arranged by size:
606 417 339 87
PciI A'CATG_T
Cuts at: 0 413 1449
Size: 413 1036
PfoI T'CCnGG_A
Cuts at: 0 230 332 695 1449
Size: 230 102 363 754
Fragments arranged by size:
754 363 230 102
PmlI CAC'GTG
Cuts at: 0 1405 1449
Size: 1405 44
PspOMI G'GGCC_C
Cuts at: 0 82 1449
Size: 82 1367
PspXI vC'TCGA_Gb
Cuts at: 0 105 1449
Size: 105 1344
PstI C_TGCA'G
Cuts at: 0 380 707 869 1082 1449
Size: 380 327 162 213 367
Fragments arranged by size:
380 367 327 213 162
PvuII CAG'CTG
Cuts at: 0 450 1110 1449
Size: 450 660 339
Fragments arranged by size:
660 450 339
SacI G_AGCT'C
Cuts at: 0 1273 1449
Size: 1273 176
SfcI C'TryA_G
Cuts at: 0 376 703 865 1078 1170 1308 1449
Size: 376 327 162 213 92 138 141
Fragments arranged by size:
376 327 213 162 141 138 92
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 974 1449
Size: 974 475
SmaI CCC'GGG
Cuts at: 0 52 1449
Size: 52 1397
SmlI C'TyrA_G
Cuts at: 0 105 1449
Size: 105 1344
StyI C'CwwG_G
Cuts at: 0 740 1449
Size: 740 709
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 667 1449
Size: 280 387 782
Fragments arranged by size:
782 387 280
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 349 381 1449
Size: 349 32 1068
Fragments arranged by size:
1068 349 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1095 1449
Size: 1095 354
XhoI C'TCGA_G
Cuts at: 0 105 1449
Size: 105 1344
XmaI C'CCGG_G
Cuts at: 0 50 1449
Size: 50 1399
ZraI GAC'GTC
Cuts at: 0 1437 1449
Size: 1437 12
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AloI AlwNI ApaI
ApoI AvaI BanII BbsI BbvCI BcgI BglI BglII
BlpI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI FspAI
Hin4I HincII MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PciI PfoI PmlI PspOMI PspXI PstI PvuII
SacI SfcI SfiI SmaI SmlI StyI TaqII TstI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BciVI BclI BfrBI BmgBI BmrI BmtI BplI
BpuEI BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII EagI EcoNI EcoRI EcoRV
FalI FseI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XmnI