Restriction map for
pFN21AB9948
(Linear) (Six-base) MAPSORT of: KIBB9948.seq Check: 2390 from: 1 to: 2019
KIBB9948.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 409 2019
Size: 409 1610
AatII G_ACGT'C
Cuts at: 0 515 2019
Size: 515 1504
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1138 1784 2019
Size: 781 357 646 235
Fragments arranged by size:
781 646 357 235
AfeI AGC'GCT
Cuts at: 0 424 2019
Size: 424 1595
AflIII A'CryG_T
Cuts at: 0 320 2019
Size: 320 1699
AleI CACnn'nnGTG
Cuts at: 0 1162 2019
Size: 1162 857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1084 2019
Size: 1084 935
AlwNI CAG_nnn'CTG
Cuts at: 0 155 2019
Size: 155 1864
ApaLI G'TGCA_C
Cuts at: 0 220 415 1155 2019
Size: 220 195 740 864
Fragments arranged by size:
864 740 220 195
ApoI r'AATT_y
Cuts at: 0 112 238 910 979 1963 1993 2019
Size: 112 126 672 69 984 30 26
Fragments arranged by size:
984 672 126 112 69 30 26
AvaI C'yCGr_G
Cuts at: 0 1528 2019
Size: 1528 491
BanI G'GyrC_C
Cuts at: 0 70 230 1614 2019
Size: 70 160 1384 405
Fragments arranged by size:
1384 405 160 70
BanII G_rGCy'C
Cuts at: 0 1100 1322 2019
Size: 1100 222 697
Fragments arranged by size:
1100 697 222
BbeI G_GCGC'C
Cuts at: 0 74 2019
Size: 74 1945
BbsI GAAGACnn'nnnn_
Cuts at: 0 637 720 2019
Size: 637 83 1299
Fragments arranged by size:
1299 637 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1519 1553 2019
Size: 1519 34 466
Fragments arranged by size:
1519 466 34
BfrBI ATG'CAT
Cuts at: 0 1792 2019
Size: 1792 227
BglI GCCn_nnn'nGGC
Cuts at: 0 452 2019
Size: 452 1567
Bme1580I G_kGCm'C
Cuts at: 0 224 419 1159 2019
Size: 224 195 740 860
Fragments arranged by size:
860 740 224 195
BmgBI CAC'GTC
Cuts at: 0 1149 2019
Size: 1149 870
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 1496 2019
Size: 312 1184 523
Fragments arranged by size:
1184 523 312
BsaI GGTCTCn'nnnn_
Cuts at: 0 995 2019
Size: 995 1024
BsaAI yAC'GTr
Cuts at: 0 323 585 1266 2019
Size: 323 262 681 753
Fragments arranged by size:
753 681 323 262
BsaHI Gr'CG_yC
Cuts at: 0 71 512 2019
Size: 71 441 1507
Fragments arranged by size:
1507 441 71
BsaWI w'CCGG_w
Cuts at: 0 1004 1799 2019
Size: 1004 795 220
Fragments arranged by size:
1004 795 220
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 958 988 2019
Size: 310 30 618 30 1031
Fragments arranged by size:
1031 618 310 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 2019
Size: 55 1964
BseYI C'CCAG_C
Cuts at: 0 442 1041 1211 1606 2019
Size: 442 599 170 395 413
Fragments arranged by size:
599 442 413 395 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 2019
Size: 141 1878
BsiEI CG_ry'CG
Cuts at: 0 480 1308 1890 2019
Size: 480 828 582 129
Fragments arranged by size:
828 582 480 129
BsiHKAI G_wGCw'C
Cuts at: 0 49 224 419 1100 1159 2019
Size: 49 175 195 681 59 860
Fragments arranged by size:
860 681 195 175 59 49
Bsp1286I G_dGCh'C
Cuts at: 0 49 224 419 1100 1159 1322 2019
Size: 49 175 195 681 59 163 697
Fragments arranged by size:
697 681 195 175 163 59 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 409 2019
Size: 409 1610
BsrFI r'CCGG_y
Cuts at: 0 1307 2019
Size: 1307 712
BstAPI GCAn_nnn'nTGC
Cuts at: 0 155 1348 2019
Size: 155 1193 671
Fragments arranged by size:
1193 671 155
BstBI TT'CG_AA
Cuts at: 0 1967 2019
Size: 1967 52
BstYI r'GATC_y
Cuts at: 0 314 576 769 1112 1835 2019
Size: 314 262 193 343 723 184
Fragments arranged by size:
723 343 314 262 193 184
Bsu36I CC'TnA_GG
Cuts at: 0 76 2019
Size: 76 1943
BtgI C'CryG_G
Cuts at: 0 474 1235 1562 2019
Size: 474 761 327 457
Fragments arranged by size:
761 474 457 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1506 2019
Size: 1506 513
BtsI GCAGTG_nn'
Cuts at: 0 153 358 2019
Size: 153 205 1661
Fragments arranged by size:
1661 205 153
DraI TTT'AAA
Cuts at: 0 2000 2019
Size: 2000 19
DrdI GACnn_nn'nnGTC
Cuts at: 0 511 1936 2019
Size: 511 1425 83
Fragments arranged by size:
1425 511 83
EaeI y'GGCC_r
Cuts at: 0 477 1305 1706 2019
Size: 477 828 401 313
Fragments arranged by size:
828 477 401 313
EagI C'GGCC_G
Cuts at: 0 477 1305 2019
Size: 477 828 714
Fragments arranged by size:
828 714 477
EarI CTCTTCn'nnn_
Cuts at: 0 1651 2019
Size: 1651 368
EcoICRI GAG'CTC
Cuts at: 0 1098 2019
Size: 1098 921
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 781 1138 1496 1784 2019
Size: 312 469 357 358 288 235
Fragments arranged by size:
469 358 357 312 288 235
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1120 2019
Size: 1120 899
EcoO109I rG'GnC_Cy
Cuts at: 0 945 2019
Size: 945 1074
EcoRI G'AATT_C
Cuts at: 0 1963 2019
Size: 1963 56
EcoRV GAT'ATC
Cuts at: 0 1779 2019
Size: 1779 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1218 2019
Size: 1218 801
HaeII r_GCGC'y
Cuts at: 0 74 266 426 474 2019
Size: 74 192 160 48 1545
Fragments arranged by size:
1545 192 160 74 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 591 623 2019
Size: 591 32 1396
Fragments arranged by size:
1396 591 32
HincII GTy'rAC
Cuts at: 0 1986 2019
Size: 1986 33
HindIII A'AGCT_T
Cuts at: 0 1859 2019
Size: 1859 160
HpaI GTT'AAC
Cuts at: 0 1986 2019
Size: 1986 33
KasI G'GCGC_C
Cuts at: 0 70 2019
Size: 70 1949
MscI TGG'CCA
Cuts at: 0 1708 2019
Size: 1708 311
MslI CAynn'nnrTG
Cuts at: 0 403 1162 2019
Size: 403 759 857
Fragments arranged by size:
857 759 403
MspA1I CmG'CkG
Cuts at: 0 476 690 2019
Size: 476 214 1329
Fragments arranged by size:
1329 476 214
NarI GG'CG_CC
Cuts at: 0 71 2019
Size: 71 1948
NcoI C'CATG_G
Cuts at: 0 1235 2019
Size: 1235 784
NotI GC'GGCC_GC
Cuts at: 0 477 2019
Size: 477 1542
NsiI A_TGCA'T
Cuts at: 0 1794 2019
Size: 1794 225
NspI r_CATG'y
Cuts at: 0 1461 1792 2019
Size: 1461 331 227
Fragments arranged by size:
1461 331 227
PflMI CCAn_nnn'nTGG
Cuts at: 0 1236 2019
Size: 1236 783
PfoI T'CCnGG_A
Cuts at: 0 947 2019
Size: 947 1072
PpuMI rG'GwC_Cy
Cuts at: 0 945 2019
Size: 945 1074
PshAI GACnn'nnGTC
Cuts at: 0 272 2019
Size: 272 1747
PsiI TTA'TAA
Cuts at: 0 1850 2019
Size: 1850 169
PstI C_TGCA'G
Cuts at: 0 151 1344 2019
Size: 151 1193 675
Fragments arranged by size:
1193 675 151
PvuII CAG'CTG
Cuts at: 0 690 2019
Size: 690 1329
SacI G_AGCT'C
Cuts at: 0 1100 2019
Size: 1100 919
SacII CC_GC'GG
Cuts at: 0 477 2019
Size: 477 1542
ScaI AGT'ACT
Cuts at: 0 853 2019
Size: 853 1166
SfcI C'TryA_G
Cuts at: 0 147 1340 2019
Size: 147 1193 679
Fragments arranged by size:
1193 679 147
SfoI GGC'GCC
Cuts at: 0 72 2019
Size: 72 1947
SnaBI TAC'GTA
Cuts at: 0 585 2019
Size: 585 1434
SphI G_CATG'C
Cuts at: 0 1792 2019
Size: 1792 227
SspI AAT'ATT
Cuts at: 0 1950 2019
Size: 1950 69
StyI C'CwwG_G
Cuts at: 0 941 1235 1694 2019
Size: 941 294 459 325
Fragments arranged by size:
941 459 325 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1033 1345 1751 1904 2019
Size: 1033 312 406 153 115
Fragments arranged by size:
1033 406 312 153 115
TatI w'GTAC_w
Cuts at: 0 851 1247 2019
Size: 851 396 772
Fragments arranged by size:
851 772 396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 378 410 1890 1922 2019
Size: 310 32 36 32 1480 32 97
Fragments arranged by size:
1480 310 97 36 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1701 2019
Size: 1701 318
XmnI GAAnn'nnTTC
Cuts at: 0 760 2019
Size: 760 1259
ZraI GAC'GTC
Cuts at: 0 513 2019
Size: 513 1506
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AlwNI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BcgI
BfrBI BglI Bme1580I BmgBI BpmI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII HpaI KasI MscI MslI MspA1I NarI NcoI
NotI NsiI NspI PflMI PfoI PpuMI PshAI PsiI
PstI PvuII SacI SacII ScaI SfcI SfoI SnaBI
SphI SspI StyI TaqII TatI TstI XcmI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstEII BstXI BstZ17I ClaI CspCI
DraIII EciI FseI FspI FspAI KpnI MfeI MluI
MmeI NaeI NdeI NgoMIV NheI NruI PacI PasI
PciI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SmlI SpeI SrfI StuI SwaI Tth111I
XbaI XhoI XmaI