(Circular) (Six-base) MAPSORT of: pFN21AB5771.seq Check: 4501 from: 1 to: 6904 pFN21AB5771 6904 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2432 3199 2432 Size: 767 6137 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4714 278 Size: 53 83 186 936 3178 2468 Fragments arranged by size: 3178 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2753 2925 4247 1937 Size: 816 172 1322 4594 Fragments arranged by size: 4594 1322 816 172 Acc65I G'GTAC_C Cuts at: 2942 4225 2942 Size: 1283 5621 AclI AA'CG_TT Cuts at: 1563 5031 5404 1563 Size: 3468 373 3063 Fragments arranged by size: 3468 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2127 3896 4967 6351 6619 1632 Size: 495 1769 1071 1384 268 1917 Fragments arranged by size: 1917 1769 1384 1071 495 268 AflII C'TTAA_G Cuts at: 820 1017 2719 820 Size: 197 1702 5005 Fragments arranged by size: 5005 1702 197 AflIII A'CryG_T Cuts at: 1203 5803 6780 1203 Size: 4600 977 1327 Fragments arranged by size: 4600 1327 977 AgeI A'CCGG_T Cuts at: 5735 5735 Size: 6904 AhdI GACnn_n'nnGTC Cuts at: 3538 5633 3538 Size: 2095 4809 AleI CACnn'nnGTG Cuts at: 1166 3777 1166 Size: 2611 4293 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3020 1184 Size: 1836 5068 AlwNI CAG_nnn'CTG Cuts at: 3026 3909 4647 6219 3026 Size: 883 738 1572 3711 Fragments arranged by size: 3711 1572 883 738 ApaLI G'TGCA_C Cuts at: 2978 4961 6117 2978 Size: 1983 1156 3765 Fragments arranged by size: 3765 1983 1156 ApoI r'AATT_y Cuts at: 1493 2324 4213 4439 4591 6700 1493 Size: 831 1889 226 152 2109 1697 Fragments arranged by size: 2109 1889 1697 831 226 152 AseI AT'TA_AT Cuts at: 160 1032 5458 160 Size: 872 4426 1606 Fragments arranged by size: 4426 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6904 AvaI C'yCGr_G Cuts at: 1870 1943 3159 3561 3743 4142 4229 1870 Size: 73 1216 402 182 399 87 4545 Fragments arranged by size: 4545 1216 402 399 182 87 73 BamHI G'GATC_C Cuts at: 4234 4601 4234 Size: 367 6537 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2398 2942 3739 4225 5680 Size: 325 217 627 611 544 797 486 1455 Cuts at: 5680 618 Size: 1842 Fragments arranged by size: 1842 1455 797 627 611 544 486 325 217 BanII G_rGCy'C Cuts at: 1391 4094 4152 4223 1391 Size: 2703 58 71 4072 Fragments arranged by size: 4072 2703 71 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2678 3144 3999 4114 928 Size: 971 779 466 855 115 3718 Fragments arranged by size: 3718 971 855 779 466 115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5095 5129 1903 Size: 34 3158 34 3678 Fragments arranged by size: 3678 3158 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4796 6006 4796 Size: 1210 5694 BclI T'GATC_A Cuts at: 1548 1548 Size: 6904 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3079 3475 5515 Size: 107 122 71 631 605 1407 396 2040 Cuts at: 5515 136 Size: 1525 Fragments arranged by size: 2040 1525 1407 631 605 396 122 107 71 BglII A'GATC_T Cuts at: 2664 3813 6899 2664 Size: 1149 3086 2669 Fragments arranged by size: 3086 2669 1149 BlpI GC'TnA_GC Cuts at: 4321 4321 Size: 6904 Bme1580I G_kGCm'C Cuts at: 1790 2403 2982 3960 4965 6121 1790 Size: 613 579 978 1005 1156 2573 Fragments arranged by size: 2573 1156 1005 978 613 579 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6904 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3897 4635 5593 Size: 416 53 83 399 24 2480 738 958 Cuts at: 5593 442 Size: 1753 Fragments arranged by size: 2480 1753 958 738 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3110 1056 Size: 2054 4850 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3018 3445 3538 3018 Size: 427 93 6384 Fragments arranged by size: 6384 427 93 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2395 2700 2882 3019 3073 5564 1239 Size: 1156 305 182 137 54 2491 2579 Fragments arranged by size: 2579 2491 1156 305 182 137 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5029 5894 6192 5029 Size: 865 298 5741 Fragments arranged by size: 5741 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3121 3597 3961 5567 882 Size: 617 1622 476 364 1606 2219 Fragments arranged by size: 2219 1622 1606 617 476 364 BsaAI yAC'GTr Cuts at: 493 1204 2757 4098 4695 493 Size: 711 1553 1341 597 2702 Fragments arranged by size: 2702 1553 1341 711 597 BsaBI GATnn'nnATC Cuts at: 2870 6898 2870 Size: 4028 2876 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4711 5093 5728 275 Size: 53 83 186 936 3178 382 635 1451 Fragments arranged by size: 3178 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3759 5337 5735 6009 6156 6567 3759 Size: 1578 398 274 147 411 4096 Fragments arranged by size: 4096 1578 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2426 2456 2549 2579 2741 Size: 30 165 30 1023 30 93 30 162 Cuts at: 2741 2771 4049 4079 1178 Size: 30 1278 30 4003 Fragments arranged by size: 4003 1278 1023 165 162 93 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 2098 2128 2162 3076 3514 3527 4093 Size: 829 83 30 34 914 438 13 566 Cuts at: 4093 4160 1186 Size: 67 3930 Fragments arranged by size: 3930 914 829 566 438 83 67 34 30 13 BseYI C'CCAG_C Cuts at: 3147 3379 3497 4070 4168 4173 6107 6603 3147 Size: 232 118 573 98 5 1934 496 3448 Fragments arranged by size: 3448 1934 573 496 232 118 98 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2044 3373 3895 1163 Size: 573 308 1329 522 4172 Fragments arranged by size: 4172 1329 573 522 308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3718 4354 5115 6143 1540 Size: 275 186 1717 636 761 1028 2301 Fragments arranged by size: 2301 1717 1028 761 636 275 186 BsiHKAI G_wGCw'C Cuts at: 2982 3804 4094 4103 4965 5050 6121 2982 Size: 822 290 9 862 85 1071 3765 Fragments arranged by size: 3765 1071 862 822 290 85 9 BsmI GAATG_Cn' Cuts at: 4415 4508 4415 Size: 93 6811 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2403 2982 3804 3960 4094 4103 4152 Size: 399 613 579 822 156 134 9 49 Cuts at: 4152 4223 4965 5050 6121 1391 Size: 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 822 742 613 579 399 156 134 85 71 49 9 BspEI T'CCGG_A Cuts at: 6567 6567 Size: 6904 BspHI T'CATG_A Cuts at: 4129 4793 6523 4129 Size: 664 1730 4510 Fragments arranged by size: 4510 1730 664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2432 2658 3199 3384 3450 3462 4259 844 Size: 1588 226 541 185 66 12 797 3489 Fragments arranged by size: 3489 1588 797 541 226 185 66 12 BsrBI CCG'CTC Cuts at: 1394 1823 2485 4791 1394 Size: 429 662 2306 3507 Fragments arranged by size: 3507 2306 662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5399 5573 65 Size: 1183 4151 174 1396 Fragments arranged by size: 4151 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2242 2496 2833 5548 5735 1700 Size: 110 432 254 337 2715 187 2869 Fragments arranged by size: 2869 2715 432 337 254 187 110 BsrGI T'GTAC_A Cuts at: 96 2574 3924 96 Size: 2478 1350 3076 Fragments arranged by size: 3076 2478 1350 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6904 BssSI C'ACGA_G Cuts at: 4964 5976 4964 Size: 1012 5892 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 3955 794 Size: 1445 1716 3743 Fragments arranged by size: 3743 1716 1445 BstBI TT'CG_AA Cuts at: 5708 5708 Size: 6904 BstEII G'GTnAC_C Cuts at: 1184 3884 1184 Size: 2700 4204 BstXI CCAn_nnnn'nTGG Cuts at: 2345 3535 4054 2345 Size: 1190 519 5195 Fragments arranged by size: 5195 1190 519 BstYI r'GATC_y Cuts at: 1972 2664 3762 3813 4234 4601 4986 5003 6455 Size: 692 1098 51 421 367 385 17 1452 Cuts at: 6455 6541 6553 6899 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1098 692 421 385 367 346 86 51 17 12 BstZ17I GTA'TAC Cuts at: 2754 2754 Size: 6904 Bsu36I CC'TnA_GG Cuts at: 4105 4105 Size: 6904 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6352 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6165 Fragments arranged by size: 6165 683 56 BtsI GCAGTG_nn' Cuts at: 2244 2297 2844 3960 4420 5214 5234 2244 Size: 53 547 1116 460 794 20 3914 Fragments arranged by size: 3914 1116 794 547 460 53 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6904 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6869 DraI TTT'AAA Cuts at: 4208 4208 Size: 6904 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3170 5911 809 Size: 507 1854 2741 1802 Fragments arranged by size: 2741 1854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2988 3071 3715 Size: 54 1425 50 275 18 1158 83 644 Cuts at: 3715 4351 5240 6869 8 Size: 636 889 1629 43 Fragments arranged by size: 1629 1425 1158 889 644 636 275 83 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 4351 1537 Size: 275 1903 636 4090 Fragments arranged by size: 4090 1903 636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 2146 2820 2930 2945 4834 1353 Size: 680 113 674 110 15 1889 3423 Fragments arranged by size: 3423 1889 680 674 113 110 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4203 5491 5865 6011 387 Size: 1064 138 2614 1288 374 146 1280 Fragments arranged by size: 2614 1288 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4092 4092 Size: 6904 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 2395 2700 2882 3019 3073 3896 Size: 393 495 268 305 182 137 54 823 Cuts at: 3896 4967 5564 6351 6619 1239 Size: 1071 597 787 268 1524 Fragments arranged by size: 1524 1071 823 787 597 495 393 305 268 268 182 137 54 EcoNI CCTnn'n_nnAGG Cuts at: 3191 3191 Size: 6904 EcoO109I rG'GnC_Cy Cuts at: 2283 2886 3384 3468 3532 2283 Size: 603 498 84 64 5655 Fragments arranged by size: 5655 603 498 84 64 EcoRI G'AATT_C Cuts at: 2324 4213 4591 2324 Size: 1889 378 4637 Fragments arranged by size: 4637 1889 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2974 2974 Size: 6904 FspI TGC'GCA Cuts at: 3008 5410 3008 Size: 2402 4502 HaeII r_GCGC'y Cuts at: 1678 6051 1678 Size: 4373 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2123 2152 2155 3308 Size: 32 135 32 548 3 29 3 1153 Cuts at: 3308 3340 3524 3556 3773 3805 3837 4049 4081 Size: 32 184 32 217 32 32 212 32 Cuts at: 4081 5545 5577 5619 5651 1373 Size: 1464 32 42 32 2626 Fragments arranged by size: 2626 1464 1153 548 217 212 184 135 42 32 32 32 32 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2805 4248 4494 669 Size: 988 281 867 1443 246 3079 Fragments arranged by size: 3079 1443 988 867 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6904 HpaI GTT'AAC Cuts at: 4494 4494 Size: 6904 KpnI G_GTAC'C Cuts at: 2946 4229 2946 Size: 1283 5621 MfeI C'AATT_G Cuts at: 4503 4503 Size: 6904 MluI A'CGCG_T Cuts at: 6780 6780 Size: 6904 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3270 4047 6018 6202 1305 Size: 1965 777 1971 184 2007 Fragments arranged by size: 2007 1971 1965 777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2990 10 Size: 54 1425 343 1158 3924 Fragments arranged by size: 3924 1425 1158 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 2618 3777 3932 4862 5221 5380 Size: 648 1218 234 1159 155 930 359 159 Cuts at: 5380 518 Size: 2042 Fragments arranged by size: 2042 1218 1159 930 648 359 234 159 155 MspA1I CmG'CkG Cuts at: 1606 2470 3985 4138 4320 4997 6145 6390 1606 Size: 864 1515 153 182 677 1148 245 2120 Fragments arranged by size: 2120 1515 1148 864 677 245 182 153 NaeI GCC'GGC Cuts at: 2244 2835 2244 Size: 591 6313 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6352 NdeI CA'TA_TG Cuts at: 387 387 Size: 6904 NgoMIV G'CCGG_C Cuts at: 2242 2833 2242 Size: 591 6313 NheI G'CTAG_C Cuts at: 1052 3106 1052 Size: 2054 4850 NotI GC'GGCC_GC Cuts at: 4351 4351 Size: 6904 NspI r_CATG'y Cuts at: 1132 1229 2237 3953 4262 5807 1132 Size: 97 1008 1716 309 1545 2229 Fragments arranged by size: 2229 1716 1545 1008 309 97 PasI CC'CwG_GG Cuts at: 1348 2542 3187 3473 1348 Size: 1194 645 286 4779 Fragments arranged by size: 4779 1194 645 286 PciI A'CATG_T Cuts at: 5803 5803 Size: 6904 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6904 PmeI GTTT'AAAC Cuts at: 4208 4208 Size: 6904 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4973 5005 6486 6518 4973 Size: 32 1481 32 5359 Fragments arranged by size: 5359 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2283 3532 2283 Size: 1249 5655 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6904 PsiI TTA'TAA Cuts at: 4474 4474 Size: 6904 PstI C_TGCA'G Cuts at: 830 3459 4256 830 Size: 2629 797 3478 Fragments arranged by size: 3478 2629 797 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6904 PvuII CAG'CTG Cuts at: 2470 3985 2470 Size: 1515 5389 SacI G_AGCT'C Cuts at: 4094 4094 Size: 6904 SalI G'TCGA_C Cuts at: 1936 4246 1936 Size: 2310 4594 SanDI GG'GwC_CC Cuts at: 2283 2283 Size: 6904 SbfI CC_TGCA'GG Cuts at: 3459 4256 3459 Size: 797 6107 ScaI AGT'ACT Cuts at: 4064 5152 6864 4064 Size: 1088 1712 4104 Fragments arranged by size: 4104 1712 1088 SexAI A'CCwGG_T Cuts at: 3534 3552 3534 Size: 18 6886 SfcI C'TryA_G Cuts at: 826 1045 3455 4252 5387 6068 6259 826 Size: 219 2410 797 1135 681 191 1471 Fragments arranged by size: 2410 1471 1135 797 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6904 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6904 SmaI CCC'GGG Cuts at: 1872 4231 1872 Size: 2359 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2719 5008 5909 6171 820 Size: 197 926 776 2289 901 262 1553 Fragments arranged by size: 2289 1553 926 901 776 262 197 SnaBI TAC'GTA Cuts at: 493 4695 493 Size: 4202 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6904 SphI G_CATG'C Cuts at: 1132 2237 3953 4262 1132 Size: 1105 1716 309 3774 Fragments arranged by size: 3774 1716 1105 309 SspI AAT'ATT Cuts at: 5 52 4828 5 Size: 47 4776 2081 Fragments arranged by size: 4776 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6904 StyI C'CwwG_G Cuts at: 513 1065 3432 3528 4343 513 Size: 552 2367 96 815 3074 Fragments arranged by size: 3074 2367 815 552 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2905 3785 4182 4931 4948 5101 5286 Size: 1255 1325 880 397 749 17 153 185 Cuts at: 5286 5731 325 Size: 445 1498 Fragments arranged by size: 1498 1325 1255 880 749 445 397 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2574 3924 4062 Size: 274 80 33 51 1443 597 1350 138 Cuts at: 4062 5150 6862 96 Size: 1088 1712 138 Fragments arranged by size: 1712 1443 1350 1088 597 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2549 2581 2741 2773 6864 6896 2549 Size: 32 160 32 4091 32 2557 Fragments arranged by size: 4091 2557 160 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6904 XbaI T'CTAG_A Cuts at: 4240 4240 Size: 6904 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2533 3622 4181 2533 Size: 1089 559 5256 Fragments arranged by size: 5256 1089 559 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6904 XmaI C'CCGG_G Cuts at: 1870 4229 1870 Size: 2359 4545 XmnI GAAnn'nnTTC Cuts at: 5033 5033 Size: 6904 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4712 276 Size: 53 83 186 936 3178 2468 Fragments arranged by size: 3178 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI