(Circular) (Six-base) MAPSORT of: pFN21AB5035.seq Check: 5417 from: 1 to: 5281 pFN21AB5035 5281 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3091 278 Size: 53 83 186 936 1555 2468 Fragments arranged by size: 2468 1555 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2624 1937 Size: 687 4594 Acc65I G'GTAC_C Cuts at: 2602 2602 Size: 5281 AclI AA'CG_TT Cuts at: 1563 3408 3781 1563 Size: 1845 373 3063 Fragments arranged by size: 3063 1845 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 2562 3344 4728 4996 1632 Size: 747 183 782 1384 268 1917 Fragments arranged by size: 1917 1384 782 747 268 183 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5084 AflIII A'CryG_T Cuts at: 1203 4180 5157 1203 Size: 2977 977 1327 Fragments arranged by size: 2977 1327 977 AgeI A'CCGG_T Cuts at: 4112 4112 Size: 5281 AhdI GACnn_n'nnGTC Cuts at: 4010 4010 Size: 5281 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5281 AlwNI CAG_nnn'CTG Cuts at: 3024 4596 3024 Size: 1572 3709 ApaLI G'TGCA_C Cuts at: 3338 4494 3338 Size: 1156 4125 ApoI r'AATT_y Cuts at: 1493 2290 2590 2816 2968 5077 1493 Size: 797 300 226 152 2109 1697 Fragments arranged by size: 2109 1697 797 300 226 152 AseI AT'TA_AT Cuts at: 160 1032 3835 160 Size: 872 2803 1606 Fragments arranged by size: 2803 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5281 AvaI C'yCGr_G Cuts at: 1870 1943 2285 2606 1870 Size: 73 342 321 4545 Fragments arranged by size: 4545 342 321 73 BamHI G'GATC_C Cuts at: 2611 2978 2611 Size: 367 4914 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2602 4057 618 Size: 325 217 627 815 1455 1842 Fragments arranged by size: 1842 1455 815 627 325 217 BanII G_rGCy'C Cuts at: 1391 2015 2140 2600 1391 Size: 624 125 460 4072 Fragments arranged by size: 4072 624 460 125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4310 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3472 3506 1903 Size: 34 50 34 1451 34 3678 Fragments arranged by size: 3678 1451 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3173 4383 3173 Size: 1210 4071 BclI T'GATC_A Cuts at: 1548 1548 Size: 5281 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3892 136 Size: 107 122 71 631 605 2220 1525 Fragments arranged by size: 2220 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2102 5276 2102 Size: 3174 2107 BlpI GC'TnA_GC Cuts at: 2698 2698 Size: 5281 Bme1580I G_kGCm'C Cuts at: 1790 2059 3342 4498 1790 Size: 269 1283 1156 2573 Fragments arranged by size: 2573 1283 1156 269 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5281 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3012 3970 442 Size: 416 53 83 399 24 1595 958 1753 Fragments arranged by size: 1753 1595 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5281 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2563 2563 Size: 5281 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2533 3941 1239 Size: 1294 1408 2579 Fragments arranged by size: 2579 1408 1294 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3406 4271 4569 3406 Size: 865 298 4118 Fragments arranged by size: 4118 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3944 882 Size: 617 2445 2219 Fragments arranged by size: 2445 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3072 493 Size: 711 1868 2702 Fragments arranged by size: 2702 1868 711 BsaBI GATnn'nnATC Cuts at: 5275 5275 Size: 5281 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3088 3470 4105 275 Size: 53 83 186 936 1555 382 635 1451 Fragments arranged by size: 1555 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3714 4112 4386 4533 4944 3714 Size: 398 274 147 411 4051 Fragments arranged by size: 4051 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5056 Fragments arranged by size: 5056 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 1186 Size: 1249 4032 BseYI C'CCAG_C Cuts at: 4484 4980 4484 Size: 496 4785 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2179 1163 Size: 573 443 4265 Fragments arranged by size: 4265 573 443 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2731 3492 4520 1540 Size: 275 186 730 761 1028 2301 Fragments arranged by size: 2301 1028 761 730 275 186 BsiHKAI G_wGCw'C Cuts at: 2140 3342 3427 4498 2140 Size: 1202 85 1071 2923 Fragments arranged by size: 2923 1202 1071 85 BsmI GAATG_Cn' Cuts at: 2026 2792 2885 2026 Size: 766 93 4422 Fragments arranged by size: 4422 766 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2059 2140 2600 3342 3427 4498 Size: 399 225 44 81 460 742 85 1071 Cuts at: 4498 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 460 399 225 85 81 44 BspEI T'CCGG_A Cuts at: 4944 4944 Size: 5281 BspHI T'CATG_A Cuts at: 3170 4900 3170 Size: 1730 3551 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2636 844 Size: 1792 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3168 1394 Size: 429 1345 3507 Fragments arranged by size: 3507 1345 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3776 3950 65 Size: 1183 2528 174 1396 Fragments arranged by size: 2528 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3925 4112 1700 Size: 110 2115 187 2869 Fragments arranged by size: 2869 2115 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5281 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5281 BssSI C'ACGA_G Cuts at: 3341 4353 3341 Size: 1012 4269 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5281 BstBI TT'CG_AA Cuts at: 4085 4085 Size: 5281 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5281 BstYI r'GATC_y Cuts at: 1972 2102 2611 2978 3363 3380 4832 4918 4930 Size: 130 509 367 385 17 1452 86 12 Cuts at: 4930 5276 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 509 385 367 346 130 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2346 2346 Size: 5281 BtgI C'CryG_G Cuts at: 513 1065 2039 2458 513 Size: 552 974 419 3336 Fragments arranged by size: 3336 974 552 419 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4542 Fragments arranged by size: 4542 683 56 BtsI GCAGTG_nn' Cuts at: 2091 2797 3591 3611 2091 Size: 706 794 20 3761 Fragments arranged by size: 3761 794 706 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5281 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2028 2063 529 Size: 35 1464 35 3747 Fragments arranged by size: 3747 1464 35 35 DraI TTT'AAA Cuts at: 2316 2585 2316 Size: 269 5012 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4288 809 Size: 507 2972 1802 Fragments arranged by size: 2972 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2728 3617 5246 Size: 54 1425 50 275 18 898 889 1629 Cuts at: 5246 8 Size: 43 Fragments arranged by size: 1629 1425 898 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2728 1537 Size: 275 916 4090 Fragments arranged by size: 4090 916 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3211 1353 Size: 1858 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3868 4242 4388 387 Size: 1064 138 2279 374 146 1280 Fragments arranged by size: 2279 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2138 2138 Size: 5281 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2533 2562 3344 3941 4728 4996 Size: 393 747 154 29 782 597 787 268 Cuts at: 4996 1239 Size: 1524 Fragments arranged by size: 1524 787 782 747 597 393 268 154 29 EcoO109I rG'GnC_Cy Cuts at: 2342 2342 Size: 5281 EcoRI G'AATT_C Cuts at: 2590 2968 2590 Size: 378 4903 FspI TGC'GCA Cuts at: 3787 3787 Size: 5281 HaeII r_GCGC'y Cuts at: 1678 4428 1678 Size: 2750 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3922 3954 3996 4028 1373 Size: 32 135 32 2350 32 42 32 2626 Fragments arranged by size: 2626 2350 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2625 2871 669 Size: 988 281 687 246 3079 Fragments arranged by size: 3079 988 687 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5281 HpaI GTT'AAC Cuts at: 2871 2871 Size: 5281 KpnI G_GTAC'C Cuts at: 2606 2606 Size: 5281 MfeI C'AATT_G Cuts at: 2880 2880 Size: 5281 MluI A'CGCG_T Cuts at: 5157 5157 Size: 5281 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4395 4579 1305 Size: 3090 184 2007 Fragments arranged by size: 3090 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3459 Fragments arranged by size: 3459 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3239 3598 3757 518 Size: 648 2073 359 159 2042 Fragments arranged by size: 2073 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2073 2335 2697 3374 4522 4767 1606 Size: 467 262 362 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 467 362 262 245 NcoI C'CATG_G Cuts at: 513 1065 2458 513 Size: 552 1393 3336 Fragments arranged by size: 3336 1393 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5281 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5281 NotI GC'GGCC_GC Cuts at: 2728 2728 Size: 5281 NspI r_CATG'y Cuts at: 1132 1229 2639 4184 1132 Size: 97 1410 1545 2229 Fragments arranged by size: 2229 1545 1410 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5281 PciI A'CATG_T Cuts at: 4180 4180 Size: 5281 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5281 PmeI GTTT'AAAC Cuts at: 2585 2585 Size: 5281 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3350 3382 4863 4895 3350 Size: 32 1481 32 3736 Fragments arranged by size: 3736 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2342 2342 Size: 5281 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5281 PsiI TTA'TAA Cuts at: 2851 2851 Size: 5281 PstI C_TGCA'G Cuts at: 830 2025 2340 2633 830 Size: 1195 315 293 3478 Fragments arranged by size: 3478 1195 315 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5281 PvuII CAG'CTG Cuts at: 2335 2335 Size: 5281 SacI G_AGCT'C Cuts at: 2140 2140 Size: 5281 SalI G'TCGA_C Cuts at: 1936 2623 1936 Size: 687 4594 SbfI CC_TGCA'GG Cuts at: 2633 2633 Size: 5281 ScaI AGT'ACT Cuts at: 3529 5241 3529 Size: 1712 3569 SfcI C'TryA_G Cuts at: 826 1045 2021 2336 2629 3764 4445 4636 826 Size: 219 976 315 293 1135 681 191 1471 Fragments arranged by size: 1471 1135 976 681 315 293 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5281 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5281 SmaI CCC'GGG Cuts at: 1872 2608 1872 Size: 736 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2285 3385 4286 4548 820 Size: 197 926 342 1100 901 262 1553 Fragments arranged by size: 1553 1100 926 901 342 262 197 SnaBI TAC'GTA Cuts at: 493 3072 493 Size: 2579 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5281 SphI G_CATG'C Cuts at: 1132 2639 1132 Size: 1507 3774 SspI AAT'ATT Cuts at: 5 52 3205 5 Size: 47 3153 2081 Fragments arranged by size: 3153 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5281 StyI C'CwwG_G Cuts at: 513 1065 2458 2720 513 Size: 552 1393 262 3074 Fragments arranged by size: 3074 1393 552 262 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3308 3325 3478 3663 4108 325 Size: 1255 1728 17 153 185 445 1498 Fragments arranged by size: 1728 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3527 5239 96 Size: 274 80 33 51 1443 1550 1712 138 Fragments arranged by size: 1712 1550 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5241 5273 5241 Size: 32 5249 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5281 XbaI T'CTAG_A Cuts at: 2617 2617 Size: 5281 XhoI C'TCGA_G Cuts at: 1943 2285 1943 Size: 342 4939 XmaI C'CCGG_G Cuts at: 1870 2606 1870 Size: 736 4545 XmnI GAAnn'nnTTC Cuts at: 3410 3410 Size: 5281 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3089 276 Size: 53 83 186 936 1555 2468 Fragments arranged by size: 2468 1555 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI