Restriction map for
pFN21AB4172
##################################
# NO CUT ENZYMES ( 159 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BanII BbeI BbrPI BbvCI
BcgI BclI BfrI BglII BlnI
BpmI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DpnII DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KpnI
Ksp632I KspI MaeII MamI MboI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NdeI NdeII
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI Sau3AI SbfI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 472 GTCGAC GT^[AC][GT]AC
AcsI 375 AAATTT [GA]^AATT[CT]
ApoI 375 AAATTT [GA]^AATT[CT]
AseI 796 ATTAAT AT^TAAT
AsnI 796 ATTAAT AT^TAAT
AspI 475 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 561 TGGCCA TGG^CCA
BbsI 103 GAAGAC GAAGAC(2/6)
BbvI 988 GCTGC GCAGC(8/12)
BcnI 574 CCCGG CC^[GC]GG
BglI 554 GCCTAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 691 GCTCAGC GC^T[ACGT]AGC
BmyI 808 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 691 GCTCAGC GC^T[ACGT]AGC
BpuAI 103 GAAGAC GAAGAC(2/6)
BsaI 1044 GGTCTC GGTCTC(1/5)
BsiYI 814 CCTGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 814 CCTGGAACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 808 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 839 TGTACA T^GTACA
BspMI 1041 GCAGGT ACCTGC(4/8)
BsrGI 839 TGTACA T^GTACA
BstXI 477 CCAGGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 691 GCTCAGC GC^T[ACGT]AGC
EaeI 561 TGGCCA [CT]^GGCC[GA]
EcoNI 814 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 988 GCTGC GC^[ACGT]GC
HapII 575 CCGG C^CGG
HindIII 205 AAGCTT A^AGCTT
HpaII 575 CCGG C^CGG
ItaI 988 GCTGC GC^[ACGT]GC
MluNI 561 TGGCCA TGG^CCA
MscI 561 TGGCCA TGG^CCA
MslI 906 CACCAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 575 CCGG C^CGG
NciI 574 CCCGG CC^[GC]GG
NcoI 992 CCATGG C^CATGG
PflFI 475 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 796 ATTAAT AT^TAAT
PstI 1039 CTGCAG CTGCA^G
PvuII 649 CAGCTG CAG^CTG
SalI 472 GTCGAC G^TCGAC
ScaI 1035 AGTACT AGT^ACT
SexAI 476 ACCAGGT A^CC[AT]GGT
SfcI 1039 CTGCAG C^T[GA][CT]AG
SfiI 553 GGCCTAAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 839 TGTACA T^GTACA
StuI 552 AGGCCT AGG^CCT
TseI 988 GCTGC G^C[AT]GC
Tth111I 475 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 685 CCGC CCGC(-3/-1)
389 GCGG
AvaII 49 GGTCC G^G[AT]CC
682 GGACC
BanI 577 GGTGCC G^G[CT][GA]CC
809 GGCACC
BfaI 688 CTAG C^TAG
1004 CTAG
DdeI 140 CTCAG C^T[ACGT]AG
692 CTCAG
DsaI 581 CCACGG C^C[GA][CT]GG
992 CCATGG
EcoT14I 269 CCAAGG C^C[AT][AT]GG
992 CCATGG
HgaI 446 GACGC GACGC(5/10)
856 GACGC
MaeI 688 CTAG C^TAG
1004 CTAG
MboII 103 GAAGA GAAGA(8/7)
538 GAAGA
MspA1I 387 CAGCGG C[AC]G^C[GT]G
649 CAGCTG
NheI 687 GCTAGC G^CTAGC
1003 GCTAGC
NlaIII 147 CATG CATG^
993 CATG
SfaNI 274 GATGC GCATC(5/9)
370 GATGC
StyI 269 CCAAGG C^C[AT][AT]GG
992 CCATGG
TaqI 473 TCGA T^CGA
513 TCGA
TspRI 483 CACTG CA[GC]TG[ACGT][ACGT]^
594 CACTG
TthHB8I 473 TCGA T^CGA
513 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BciVI BsaJI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I EcoRII FokI HaeIII HincII
HindII HinfI HphI MaeIII MnlI
MseI MvaI MwoI NlaIV PleI
RsaI Sau96I ScrFI Tru9I Tsp45I
Tsp509I