(Circular) (Six-base) MAPSORT of: pFN21AB4172.seq Check: 1370 from: 1 to: 5782 pFN21AB4172 5782 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2478 3125 1937 Size: 541 647 4594 Fragments arranged by size: 4594 647 541 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3845 5229 5497 1632 Size: 2213 1384 268 1917 Fragments arranged by size: 2213 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 2915 1166 Size: 1749 4033 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 3525 5097 3525 Size: 1572 4210 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 2380 3091 3317 3469 5578 1493 Size: 887 711 226 152 2109 1697 Fragments arranged by size: 2109 1697 887 711 226 152 AseI AT'TA_AT Cuts at: 160 1032 2802 4336 160 Size: 872 1770 1534 1606 Fragments arranged by size: 1770 1606 1534 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 3107 1870 Size: 73 1164 4545 Fragments arranged by size: 4545 1164 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2582 2814 3103 4558 618 Size: 325 217 627 795 232 289 1455 1842 Fragments arranged by size: 1842 1455 795 627 325 289 232 217 BanII G_rGCy'C Cuts at: 1391 3101 1391 Size: 1710 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2115 928 Size: 971 216 4595 Fragments arranged by size: 4595 971 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2691 2885 3014 3674 4884 2691 Size: 194 129 660 1210 3589 Fragments arranged by size: 3589 1210 660 194 129 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2565 4393 136 Size: 107 122 71 631 605 893 1828 1525 Fragments arranged by size: 1828 1525 893 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 2697 3199 2697 Size: 502 5280 Bme1580I G_kGCm'C Cuts at: 1790 2817 3843 4999 1790 Size: 1027 1026 1156 2573 Fragments arranged by size: 2573 1156 1027 1026 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2696 3012 1056 Size: 1640 316 3826 Fragments arranged by size: 3826 1640 316 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2778 2778 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4442 1239 Size: 3203 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3055 4445 882 Size: 617 1556 1390 2219 Fragments arranged by size: 2219 1556 1390 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4215 4613 4887 5034 5445 4215 Size: 398 274 147 411 4552 Fragments arranged by size: 4552 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2176 2206 3024 3054 1178 Size: 30 165 30 773 30 818 30 3906 Fragments arranged by size: 3906 818 773 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5782 BseYI C'CCAG_C Cuts at: 2652 4985 5481 2652 Size: 2333 496 2953 Fragments arranged by size: 2953 2333 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3843 3928 4999 3843 Size: 85 1071 4626 Fragments arranged by size: 4626 1071 85 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2817 3101 3843 3928 4999 1391 Size: 399 1027 284 742 85 1071 2174 Fragments arranged by size: 2174 1071 1027 742 399 284 85 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3037 3137 844 Size: 2193 100 3489 Fragments arranged by size: 3489 2193 100 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 2844 96 Size: 2748 3034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2489 2489 Size: 5782 BstYI r'GATC_y Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777 Size: 1140 367 385 17 1452 86 12 346 Cuts at: 5777 1972 Size: 1977 Fragments arranged by size: 1977 1452 1140 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2586 2997 513 Size: 552 939 582 411 3298 Fragments arranged by size: 3298 939 582 552 411 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 3298 4092 4112 3298 Size: 794 20 4968 Fragments arranged by size: 4968 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2566 3229 4118 Size: 54 1425 50 275 18 736 663 889 Cuts at: 4118 5747 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 736 663 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3712 1353 Size: 2359 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3845 4442 5229 5497 1239 Size: 393 2213 597 787 268 1524 Fragments arranged by size: 2213 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2823 2823 Size: 5782 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2140 2194 2379 2140 Size: 54 185 5543 Fragments arranged by size: 5543 185 54 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 4929 1678 Size: 3251 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373 Size: 32 135 32 2851 32 42 32 2626 Fragments arranged by size: 2851 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2092 2134 2479 3126 3372 669 Size: 988 281 154 42 345 647 246 3079 Fragments arranged by size: 3079 988 647 345 281 246 154 42 HindIII A'AGCT_T Cuts at: 748 2210 748 Size: 1462 4320 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2081 2406 2700 4896 5080 1305 Size: 776 325 294 2196 184 2007 Fragments arranged by size: 2196 2007 776 325 294 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2568 10 Size: 54 1425 343 736 3224 Fragments arranged by size: 3224 1425 736 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2915 3740 4099 4258 518 Size: 648 1749 825 359 159 2042 Fragments arranged by size: 2042 1749 825 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2394 2656 3198 3875 5023 5268 1606 Size: 788 262 542 677 1148 245 2120 Fragments arranged by size: 2120 1148 788 677 542 262 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2997 513 Size: 552 939 993 3298 Fragments arranged by size: 3298 993 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 2692 3008 1052 Size: 1640 316 3826 Fragments arranged by size: 3826 1640 316 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 2810 1348 Size: 1462 4320 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PfoI T'CCnGG_A Cuts at: 2683 2683 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 3048 3134 830 Size: 2218 86 3478 Fragments arranged by size: 3478 2218 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2656 2656 Size: 5782 SalI G'TCGA_C Cuts at: 1936 2477 3124 1936 Size: 541 647 4594 Fragments arranged by size: 4594 647 541 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 3042 4030 5742 3042 Size: 988 1712 3082 Fragments arranged by size: 3082 1712 988 SexAI A'CCwGG_T Cuts at: 2481 2481 Size: 5782 SfcI C'TryA_G Cuts at: 826 1045 3044 3130 4265 4946 5137 826 Size: 219 1999 86 1135 681 191 1471 Fragments arranged by size: 1999 1471 1135 681 219 191 86 SfiI GGCCn_nnn'nGGCC Cuts at: 2565 2565 Size: 5782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 2559 1517 Size: 1042 4740 StyI C'CwwG_G Cuts at: 513 1065 2004 2274 2997 3221 513 Size: 552 939 270 723 224 3074 Fragments arranged by size: 3074 939 723 552 270 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3809 3826 3979 4164 4609 325 Size: 1255 2229 17 153 185 445 1498 Fragments arranged by size: 2229 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2844 3040 4028 Size: 274 80 33 51 1443 867 196 988 Cuts at: 4028 5740 96 Size: 1712 138 Fragments arranged by size: 1712 1443 988 867 274 196 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2176 2208 5742 5774 2176 Size: 32 3534 32 2184 Fragments arranged by size: 3534 2184 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2483 1316 Size: 1167 4615 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI XcmI