Restriction map for
pFN21AB3668
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanII BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraII DraIII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
HincII HindII HpaI HphI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AcyI 25 GGCGCC G[GA]^CG[CT]C
AflIII 511 ACGTGT A^C[GA][CT]GT
Asp700I 196 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 61 GAGCAC G[AT]GC[AT]^C
AvaI 14 CCCGGG C^[CT]CG[GA]G
AvaII 331 GGTCC G^G[AT]CC
BbeI 25 GGCGCC GGCGC^C
BbsI 395 GAAGAC GAAGAC(2/6)
BbvI 56 GCTGC GCAGC(8/12)
BmyI 61 GAGCAC G[AGT]GC[ACT]^C
BpmI 752 CTGGAG CTGGAG(16/14)
BpuAI 395 GAAGAC GAAGAC(2/6)
BsaHI 25 GGCGCC G[GA]^CG[CT]C
BsaJI 14 CCCGGG C^C[ACGT][ACGT]GG
BsgI 178 GTGCAG GTGCAG(16/14)
BsiHKAI 61 GAGCAC G[AT]GC[AT]^C
BsiYI 581 CCAGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 581 CCAGGTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 420 GTCCC GGGAC(10/14)
BsoBI 14 CCCGGG C^[CT]CG[GA]G
Bsp1286I 61 GAGCAC G[AGT]GC[ACT]^C
BspHI 129 TCATGA T^CATGA
BsrDI 665 GCAATG GCAATG(2/0)
BsrI 771 ACTGG ACTGG(1/-1)
BstNI 581 CCAGG CC^[AT]GG
CfoI 26 GCGC GCG^C
DraI 163 TTTAAA TTT^AAA
DrdI 425 GACAGAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 581 CCAGG ^CC[AT]GG
FauI 51 GCGGG CCCGC(4/6)
HaeII 25 GGCGCC [GA]GCGC^[CT]
HaeIII 12 GGCC GG^CC
HapII 15 CCGG C^CGG
HhaI 26 GCGC GCG^C
Hin1I 25 GGCGCC G[GA]^CG[CT]C
HinP1I 26 GCGC G^CGC
HindIII 106 AAGCTT A^AGCTT
HpaII 15 CCGG C^CGG
KasI 25 GGCGCC G^GCGCC
MseI 164 TTAA T^TAA
MslI 645 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 15 CCGG C^CGG
MvaI 581 CCAGG CC^[AT]GG
NarI 25 GGCGCC GG^CGCC
NspI 207 ACATGC [GA]CATG^[CT]
PflMI 581 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 618 GACTC GAGTC(4/5)
PvuII 595 CAGCTG CAG^CTG
RcaI 129 TCATGA T^CATGA
SexAI 580 ACCAGGT A^CC[AT]GGT
SfcI 540 CTACAG C^T[GA][CT]AG
SfoI 25 GGCGCC GGC^GCC
SmaI 14 CCCGGG CCC^GGG
SmlI 746 CTCAAG C^T[CT][GA]AG
SspI 251 AATATT AAT^ATT
Tru9I 164 TTAA T^TAA
TseI 56 GCTGC G^C[AT]GC
Tsp45I 101 GTGAC ^GT[GC]AC
Van91I 581 CCAGGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 14 CCCGGG C^CCGGG
XmaI 14 CCCGGG C^CCGGG
XmnI 196 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 683 GATCC GGATC(4/5)
774 GGATC
BanI 25 GGCGCC G^G[CT][GA]CC
90 GGCACC
BcnI 14 CCCGG CC^[GC]GG
15 CCGGG
BfaI 154 CTAG C^TAG
376 CTAG
BsmAI 591 GAGAC GTCTC(1/5)
80 GTCTC
Cac8I 28 GCCAGC GC[ACGT]^[ACGT]GC
59 GCGAGC
Cfr13I 12 GGCCC G^G[ACGT]CC
331 GGTCC
MaeI 154 CTAG C^TAG
376 CTAG
MaeII 511 ACGT A^CGT
759 ACGT
MaeIII 101 GTGAC ^GT[ACGT]AC
365 GTAAC
NciI 14 CCCGG CC^[GC]GG
15 CCGGG
NlaIV 25 GGCGCC GG[ACGT]^[ACGT]CC
90 GGCACC
Sau96I 12 GGCCC G^G[ACGT]CC
331 GGTCC
TfiI 384 GAATC G^A[AT]TC
730 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AcsI AluI ApoI BssKI
BstF5I DpnII Fnu4HI FokI HinfI
ItaI MboI MboII MnlI MspA1I
MwoI NdeII NlaIII Sau3AI ScrFI
Tsp509I TspRI