Restriction map for
pFN21AB3469
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsiYI BslI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
FauI FbaI Fnu4HI FseI FspI
HaeII HaeIII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TseI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 85 CGCG CG^CG
AcsI 193 GAATTT [GA]^AATT[CT]
AocI 532 CCTGAGG CC^T[ACGT]AGG
ApoI 193 GAATTT [GA]^AATT[CT]
AspHI 139 GAGCAC G[AT]GC[AT]^C
AvaI 341 CTCGGG C^[CT]CG[GA]G
BcgI 329 GCAATGATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 76 CTAG C^TAG
BmyI 139 GAGCAC G[AGT]GC[ACT]^C
BsiHKAI 139 GAGCAC G[AT]GC[AT]^C
BsmAI 510 GAGAC GTCTC(1/5)
BsmFI 96 GGGAC GGGAC(10/14)
BsoBI 341 CTCGGG C^[CT]CG[GA]G
Bsp1286I 139 GAGCAC G[AGT]GC[ACT]^C
BsrDI 329 GCAATG GCAATG(2/0)
BsrI 360 ACTGG ACTGG(1/-1)
BstUI 85 CGCG CG^CG
Bsu36I 532 CCTGAGG CC^T[ACGT]AGG
Cac8I 522 GCATGC GC[ACGT]^[ACGT]GC
CfoI 84 GCGC GCG^C
DpnII 375 GATC ^GATC
EarI 505 GAAGAG CTCTTC(1/4)
Eco81I 532 CCTGAGG CC^T[ACGT]AGG
EcoRV 334 GATATC GAT^ATC
EcoT22I 524 ATGCAT ATGCA^T
HhaI 84 GCGC GCG^C
HinP1I 84 GCGC G^CGC
HinfI 391 GATTC G^A[ACGT]TC
Ksp632I 505 GAAGAG CTCTTC(1/4)
MaeI 76 CTAG C^TAG
MaeIII 260 GTAAC ^GT[ACGT]AC
MboI 375 GATC ^GATC
MspA1I 23 CAGCTG C[AC]G^C[GT]G
MvnI 85 CGCG CG^CG
NdeII 375 GATC ^GATC
NsiI 524 ATGCAT ATGCA^T
NspI 522 GCATGC [GA]CATG^[CT]
PvuII 23 CAGCTG CAG^CTG
Sau3AI 375 GATC ^GATC
SexAI 446 ACCTGGT A^CC[AT]GGT
SfaNI 538 GATGC GCATC(5/9)
SphI 522 GCATGC GCATG^C
TfiI 391 GATTC G^A[AT]TC
TspRI 288 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 18 GCGG CCGC(-3/-1)
541 GCGG
BsaJI 241 CCCAGG C^C[ACGT][ACGT]GG
242 CCAGGG
BssKI 242 CCAGG ^CC[ACGT]GG
447 CCTGG
BstF5I 45 GGATG GGATG(2/0)
537 GGATG
BstNI 242 CCAGG CC^[AT]GG
447 CCTGG
EcoRII 242 CCAGG ^CC[AT]GG
447 CCTGG
FokI 45 GGATG GGATG(9/13)
537 GGATG
HphI 226 GGTGA GGTGA(8/7)
400 GGTGA
MseI 92 TTAA T^TAA
406 TTAA
MvaI 242 CCAGG CC^[AT]GG
447 CCTGG
MwoI 75 GCTAGACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
457 GCAAAAGAAGC
NlaIII 518 CATG CATG^
523 CATG
NlaIV 153 GGAACC GG[ACGT]^[ACGT]CC
363 GGAACC
ScrFI 242 CCAGG CC^[ACGT]GG
447 CCTGG
Tru9I 92 TTAA T^TAA
406 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AluI BseRI DdeI MboII MnlI
Tsp509I