(Circular) (Six-base) MAPSORT of: pFN21AB3434.seq Check: 8201 from: 1 to: 5674 pFN21AB3434 5674 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3484 278 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3017 1937 Size: 1080 4594 Acc65I G'GTAC_C Cuts at: 2679 2995 2679 Size: 316 5358 AclI AA'CG_TT Cuts at: 1563 3801 4174 1563 Size: 2238 373 3063 Fragments arranged by size: 3063 2238 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2168 2558 3737 5121 5389 1632 Size: 536 390 1179 1384 268 1917 Fragments arranged by size: 1917 1384 1179 536 390 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5477 AflIII A'CryG_T Cuts at: 1203 2394 4573 5550 1203 Size: 1191 2179 977 1327 Fragments arranged by size: 2179 1327 1191 977 AgeI A'CCGG_T Cuts at: 4505 4505 Size: 5674 AhdI GACnn_n'nnGTC Cuts at: 4403 4403 Size: 5674 AleI CACnn'nnGTG Cuts at: 1166 2408 1166 Size: 1242 4432 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5674 AlwNI CAG_nnn'CTG Cuts at: 2431 3417 4989 2431 Size: 986 1572 3116 Fragments arranged by size: 3116 1572 986 ApaLI G'TGCA_C Cuts at: 2614 2729 3731 4887 2614 Size: 115 1002 1156 3401 Fragments arranged by size: 3401 1156 1002 115 ApoI r'AATT_y Cuts at: 1493 2983 3209 3361 5470 1493 Size: 1490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 226 152 AscI GG'CGCG_CC Cuts at: 2740 2740 Size: 5674 AseI AT'TA_AT Cuts at: 160 1032 4228 160 Size: 872 3196 1606 Fragments arranged by size: 3196 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2813 2882 2999 1870 Size: 73 127 743 69 117 4545 Fragments arranged by size: 4545 743 127 117 73 69 AvrII C'CTAG_G Cuts at: 2167 2167 Size: 5674 BamHI G'GATC_C Cuts at: 3004 3371 3004 Size: 367 5307 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2108 2679 2822 2995 4450 Size: 325 217 627 321 571 143 173 1455 Cuts at: 4450 618 Size: 1842 Fragments arranged by size: 1842 1455 627 571 325 321 217 173 143 BanII G_rGCy'C Cuts at: 1391 2071 2820 2993 1391 Size: 680 749 173 4072 Fragments arranged by size: 4072 749 680 173 BbeI G_GCGC'C Cuts at: 2826 2826 Size: 5674 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4703 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3865 3899 1903 Size: 34 50 34 1844 34 3678 Fragments arranged by size: 3678 1844 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3566 4776 3566 Size: 1210 4464 BclI T'GATC_A Cuts at: 1548 1548 Size: 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2352 2719 Size: 107 122 71 631 605 337 343 367 Cuts at: 2719 4285 136 Size: 1566 1525 Fragments arranged by size: 1566 1525 631 605 367 343 337 122 107 71 BglII A'GATC_T Cuts at: 5669 5669 Size: 5674 BlpI GC'TnA_GC Cuts at: 2852 3091 2852 Size: 239 5435 Bme1580I G_kGCm'C Cuts at: 1790 2113 2618 2733 3735 4891 1790 Size: 323 505 115 1002 1156 2573 Fragments arranged by size: 2573 1156 1002 505 323 115 BmgBI CAC'GTC Cuts at: 1318 2383 1318 Size: 1065 4609 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2239 3405 4363 Size: 416 53 83 399 24 822 1166 958 Cuts at: 4363 442 Size: 1753 Fragments arranged by size: 1753 1166 958 822 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5674 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4334 1239 Size: 3095 2579 Bpu10I CC'TnA_GC Cuts at: 2592 2592 Size: 5674 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3799 4664 4962 3799 Size: 865 298 4511 Fragments arranged by size: 4511 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4337 882 Size: 617 2838 2219 Fragments arranged by size: 2838 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3465 493 Size: 711 2261 2702 Fragments arranged by size: 2702 2261 711 BsaBI GATnn'nnATC Cuts at: 2196 5668 2196 Size: 3472 2202 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2823 2944 3481 3863 Size: 53 83 186 936 1290 121 537 382 Cuts at: 3863 4498 275 Size: 635 1451 Fragments arranged by size: 1451 1290 936 635 537 382 186 121 83 53 BsaWI w'CCGG_w Cuts at: 4107 4505 4779 4926 5337 4107 Size: 398 274 147 411 4444 Fragments arranged by size: 4444 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2900 2930 1178 Size: 30 165 30 1497 30 3922 Fragments arranged by size: 3922 1497 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2135 2255 2276 2279 1186 Size: 946 3 120 21 3 4581 Fragments arranged by size: 4581 946 120 21 3 3 BseYI C'CCAG_C Cuts at: 2043 2100 2180 2297 2722 2826 2831 4877 5373 Size: 57 80 117 425 104 5 2046 496 Cuts at: 5373 2043 Size: 2344 Fragments arranged by size: 2344 2046 496 425 117 104 80 57 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2354 2363 2375 1163 Size: 573 618 9 12 4462 Fragments arranged by size: 4462 618 573 12 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2521 2737 3124 3885 4913 1540 Size: 275 186 520 216 387 761 1028 2301 Fragments arranged by size: 2301 1028 761 520 387 275 216 186 BsiHKAI G_wGCw'C Cuts at: 2517 2618 2733 2820 3735 3820 4891 2517 Size: 101 115 87 915 85 1071 3300 Fragments arranged by size: 3300 1071 915 115 101 87 85 BsmI GAATG_Cn' Cuts at: 3185 3278 3185 Size: 93 5581 BsmBI CGTCTCn'nnnn_ Cuts at: 2951 2951 Size: 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2113 2517 2618 2733 2820 2993 Size: 399 281 42 404 101 115 87 173 Cuts at: 2993 3735 3820 4891 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 404 399 281 173 115 101 87 85 42 BspEI T'CCGG_A Cuts at: 5337 5337 Size: 5674 BspHI T'CATG_A Cuts at: 3563 5293 3563 Size: 1730 3944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2691 3029 844 Size: 1847 338 3489 Fragments arranged by size: 3489 1847 338 BsrBI CCG'CTC Cuts at: 1394 1823 2125 2851 3561 1394 Size: 429 302 726 710 3507 Fragments arranged by size: 3507 726 710 429 302 BsrDI GCAATG_nn' Cuts at: 65 1248 4169 4343 65 Size: 1183 2921 174 1396 Fragments arranged by size: 2921 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2351 2644 4318 4505 1700 Size: 110 541 293 1674 187 2869 Fragments arranged by size: 2869 1674 541 293 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5674 BssHII G'CGCG_C Cuts at: 1925 2686 2740 1925 Size: 761 54 4859 Fragments arranged by size: 4859 761 54 BssSI C'ACGA_G Cuts at: 3734 4746 3734 Size: 1012 4662 BstAPI GCAn_nnn'nTGC Cuts at: 794 2377 2482 794 Size: 1583 105 3986 Fragments arranged by size: 3986 1583 105 BstBI TT'CG_AA Cuts at: 2586 2763 4478 2586 Size: 177 1715 3782 Fragments arranged by size: 3782 1715 177 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5674 BstYI r'GATC_y Cuts at: 1972 3004 3371 3756 3773 5225 5311 5323 5669 Size: 1032 367 385 17 1452 86 12 346 Cuts at: 5669 1972 Size: 1977 Fragments arranged by size: 1977 1452 1032 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2316 2316 Size: 5674 BtgI C'CryG_G Cuts at: 513 1065 2062 513 Size: 552 997 4125 Fragments arranged by size: 4125 997 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2481 2783 487 Size: 683 56 1255 302 3378 Fragments arranged by size: 3378 1255 683 302 56 BtsI GCAGTG_nn' Cuts at: 3190 3984 4004 3190 Size: 794 20 4860 Fragments arranged by size: 4860 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5674 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5639 DraI TTT'AAA Cuts at: 2978 2978 Size: 5674 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4681 809 Size: 507 3365 1802 Fragments arranged by size: 3365 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2059 2349 2518 Size: 54 1425 50 275 18 229 290 169 Cuts at: 2518 2770 3121 4010 5639 8 Size: 252 351 889 1629 43 Fragments arranged by size: 1629 1425 889 351 290 275 252 229 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2518 3121 1537 Size: 275 706 603 4090 Fragments arranged by size: 4090 706 603 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2253 2765 3604 1353 Size: 900 512 839 3423 Fragments arranged by size: 3423 900 839 512 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4261 4635 4781 387 Size: 1064 138 2672 374 146 1280 Fragments arranged by size: 2672 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2818 2818 Size: 5674 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2558 3737 4334 5121 5389 1239 Size: 393 536 390 1179 597 787 268 1524 Fragments arranged by size: 1524 1179 787 597 536 393 390 268 EcoNI CCTnn'n_nnAGG Cuts at: 2486 2486 Size: 5674 EcoO109I rG'GnC_Cy Cuts at: 2137 2749 2137 Size: 612 5062 EcoRI G'AATT_C Cuts at: 2983 3361 2983 Size: 378 5296 FseI GG_CCGG'CC Cuts at: 2355 2355 Size: 5674 FspI TGC'GCA Cuts at: 4180 4180 Size: 5674 HaeII r_GCGC'y Cuts at: 1678 2672 2696 2826 2838 4821 1678 Size: 994 24 130 12 1983 2531 Fragments arranged by size: 2531 1983 994 130 24 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2900 2932 4315 4347 4389 Size: 32 135 32 1328 32 1383 32 42 Cuts at: 4389 4421 1373 Size: 32 2626 Fragments arranged by size: 2626 1383 1328 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3018 3264 669 Size: 988 281 1080 246 3079 Fragments arranged by size: 3079 1080 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5674 HpaI GTT'AAC Cuts at: 3264 3264 Size: 5674 KasI G'GCGC_C Cuts at: 2822 2822 Size: 5674 KpnI G_GTAC'C Cuts at: 2683 2999 2683 Size: 316 5358 MfeI C'AATT_G Cuts at: 3273 3273 Size: 5674 MluI A'CGCG_T Cuts at: 2394 5550 2394 Size: 3156 2518 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 4788 4972 1305 Size: 1074 2409 184 2007 Fragments arranged by size: 2409 2007 1074 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2061 10 Size: 54 1425 343 229 3623 Fragments arranged by size: 3623 1425 343 229 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 3632 3991 4150 518 Size: 648 1242 1224 359 159 2042 Fragments arranged by size: 2042 1242 1224 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2377 2440 2941 3090 3767 4915 5160 1606 Size: 771 63 501 149 677 1148 245 2120 Fragments arranged by size: 2120 1148 771 677 501 245 149 63 NaeI GCC'GGC Cuts at: 2353 2646 2353 Size: 293 5381 NarI GG'CG_CC Cuts at: 2823 2823 Size: 5674 NcoI C'CATG_G Cuts at: 513 1065 2062 513 Size: 552 997 4125 Fragments arranged by size: 4125 997 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5674 NgoMIV G'CCGG_C Cuts at: 2351 2644 2351 Size: 293 5381 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5674 NotI GC'GGCC_GC Cuts at: 3121 3121 Size: 5674 NspI r_CATG'y Cuts at: 1132 1229 3032 4577 1132 Size: 97 1803 1545 2229 Fragments arranged by size: 2229 1803 1545 97 PasI CC'CwG_GG Cuts at: 1348 2425 1348 Size: 1077 4597 PciI A'CATG_T Cuts at: 4573 4573 Size: 5674 PflMI CCAn_nnn'nTGG Cuts at: 1108 2050 2662 1108 Size: 942 612 4120 Fragments arranged by size: 4120 942 612 PmeI GTTT'AAAC Cuts at: 2978 2978 Size: 5674 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3743 3775 5256 5288 3743 Size: 32 1481 32 4129 Fragments arranged by size: 4129 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5674 PsiI TTA'TAA Cuts at: 3244 3244 Size: 5674 PstI C_TGCA'G Cuts at: 830 2160 2487 3026 830 Size: 1330 327 539 3478 Fragments arranged by size: 3478 1330 539 327 PvuI CG_AT'CG Cuts at: 2001 2737 2001 Size: 736 4938 PvuII CAG'CTG Cuts at: 2377 2440 2377 Size: 63 5611 SacI G_AGCT'C Cuts at: 2820 2820 Size: 5674 SalI G'TCGA_C Cuts at: 1936 3016 1936 Size: 1080 4594 SbfI CC_TGCA'GG Cuts at: 2487 3026 2487 Size: 539 5135 ScaI AGT'ACT Cuts at: 3922 5634 3922 Size: 1712 3962 SfcI C'TryA_G Cuts at: 826 1045 2156 2483 3022 4157 4838 5029 826 Size: 219 1111 327 539 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 539 327 219 191 SfoI GGC'GCC Cuts at: 2824 2824 Size: 5674 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5674 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5674 SmaI CCC'GGG Cuts at: 1872 3001 1872 Size: 1129 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2882 3778 4679 4941 820 Size: 197 926 939 896 901 262 1553 Fragments arranged by size: 1553 939 926 901 896 262 197 SnaBI TAC'GTA Cuts at: 493 3465 493 Size: 2972 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5674 SphI G_CATG'C Cuts at: 1132 3032 1132 Size: 1900 3774 SspI AAT'ATT Cuts at: 5 52 3598 5 Size: 47 3546 2081 Fragments arranged by size: 3546 2081 47 StuI AGG'CCT Cuts at: 1517 2367 2903 1517 Size: 850 536 4288 Fragments arranged by size: 4288 850 536 StyI C'CwwG_G Cuts at: 513 1065 2062 2167 3113 513 Size: 552 997 105 946 3074 Fragments arranged by size: 3074 997 946 552 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3701 3718 3871 4056 4501 325 Size: 1255 2121 17 153 185 445 1498 Fragments arranged by size: 2121 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3920 5632 96 Size: 274 80 33 51 1443 1943 1712 138 Fragments arranged by size: 1943 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5634 5666 5634 Size: 32 5642 Tth111I GACn'n_nGTC Cuts at: 1316 2324 1316 Size: 1008 4666 XbaI T'CTAG_A Cuts at: 3010 3010 Size: 5674 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2870 2870 Size: 5674 XhoI C'TCGA_G Cuts at: 1943 2882 1943 Size: 939 4735 XmaI C'CCGG_G Cuts at: 1870 2999 1870 Size: 1129 4545 XmnI GAAnn'nnTTC Cuts at: 3803 3803 Size: 5674 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3482 276 Size: 53 83 186 936 1948 2468 Fragments arranged by size: 2468 1948 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI