Restriction map for
pFN21AB9771
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AgeI AhdI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MspA1I MvnI NarI NdeI NheI
NotI NruI NsiI NspV PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 1564 GCGG CCGC(-3/-1)
AclI 460 AACGTT AA^CGTT
Alw44I 188 GTGCAC G^TGCAC
AocI 1216 CCTGAGG CC^T[ACGT]AGG
ApaLI 188 GTGCAC G^TGCAC
AspHI 188 GTGCAC G[AT]GC[AT]^C
AvaI 7 CTCGAG C^[CT]CG[GA]G
BbsI 1225 GAAGAC GAAGAC(2/6)
BbvCI 1319 CCTCAGC CCTCAGC(-5/-2)
BcgI 909 GCATCCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1295 CTAG C^TAG
BpuAI 1225 GAAGAC GAAGAC(2/6)
BsaWI 1257 ACCGGA [AT]^CCGG[AT]
BsgI 1558 GTGCAG GTGCAG(16/14)
BsiHKAI 188 GTGCAC G[AT]GC[AT]^C
BsoBI 7 CTCGAG C^[CT]CG[GA]G
Bsp1407I 827 TGTACA T^GTACA
BspMI 802 GCAGGT ACCTGC(4/8)
BsrGI 827 TGTACA T^GTACA
BstNI 855 CCTGG CC^[AT]GG
BstXI 941 CCAGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 549 GGATCT [GA]^GATC[CT]
Bsu36I 1216 CCTGAGG CC^T[ACGT]AGG
DraI 1495 TTTAAA TTT^AAA
EaeI 135 TGGCCG [CT]^GGCC[GA]
Eco81I 1216 CCTGAGG CC^T[ACGT]AGG
EcoRII 855 CCTGG ^CC[AT]GG
FseI 1453 GGCCGGCC GGCCGG^CC
HincII 402 GTTGAC GT[CT]^[GA]AC
HindII 402 GTTGAC GT[CT]^[GA]AC
MaeI 1295 CTAG C^TAG
MaeII 461 ACGT A^CGT
MfeI 608 CAATTG C^AATTG
MflI 549 GGATCT [GA]^GATC[CT]
MslI 1131 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 608 CAATTG C^AATTG
MvaI 855 CCTGG CC^[AT]GG
NaeI 1454 GCCGGC GCC^GGC
NgoMIV 1454 GCCGGC G^CCGGC
PacI 640 TTAATTAA TTAAT^TAA
PaeR7I 7 CTCGAG C^TCGAG
Psp1406I 460 AACGTT AA^CGTT
PstI 1106 CTGCAG CTGCA^G
SexAI 854 ACCTGGT A^CC[AT]GGT
SmlI 7 CTCGAG C^T[CT][GA]AG
SphI 819 GCATGC GCATG^C
SspBI 827 TGTACA T^GTACA
XhoI 7 CTCGAG C^TCGAG
XhoII 549 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 828 GTAC GT^AC
1384 GTAC
AflIII 423 ACATGT A^C[GA][CT]GT
694 ACATGT
AlwNI 803 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1094 CAGGGGCTG
BcnI 620 CCCGG CC^[GC]GG
812 CCCGG
BsaJI 757 CCATGG C^C[ACGT][ACGT]GG
870 CCATGG
BspLU11I 423 ACATGT A^CATGT
694 ACATGT
BsrDI 171 CATTGC GCAATG(2/0)
1209 CATTGC
BsrFI 435 GCCGGT [GA]^CCGG[CT]
1454 GCCGGC
BstAPI 726 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
802 GCAGGTTCTGC
Cfr10I 435 GCCGGT [GA]^CCGG[CT]
1454 GCCGGC
DraII 751 AGGACCC [GA]G^G[ACGT]CC[CT]
1330 GGGTCCC
DsaI 757 CCATGG C^C[GA][CT]GG
870 CCATGG
EcoNI 92 CCTTTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1531 CCTCTGGAAGG
EcoO109I 751 AGGACCC [GA]G^G[ACGT]CC[CT]
1330 GGGTCCC
EcoT14I 757 CCATGG C^C[AT][AT]GG
870 CCATGG
NciI 620 CCCGG CC^[GC]GG
812 CCCGG
NcoI 757 CCATGG C^CATGG
870 CCATGG
PleI 448 GACTC GAGTC(4/5)
19 GAGTC
PpuMI 751 AGGACCC [GA]G^G[AT]CC[CT]
1330 GGGTCCC
RsaI 828 GTAC GT^AC
1384 GTAC
SapI 840 GAAGAGC GCTCTTC(1/4)
1171 GAAGAGC
SfcI 1106 CTGCAG C^T[GA][CT]AG
1158 CTGTAG
StyI 757 CCATGG C^C[AT][AT]GG
870 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AluI AlwI AvaII BbvI BmyI
BpmI BseRI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BssSI BstF5I
Cac8I Cfr13I DdeI DpnII EarI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MspI
MwoI NdeII NlaIII NlaIV NspI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I