(Circular) (Six-base) MAPSORT of: pFN21AB9771.seq Check: 9197 from: 1 to: 6319 pFN21AB9771 6319 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 2466 4446 4819 1563 Size: 903 1980 373 3063 Fragments arranged by size: 3063 1980 903 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2554 4382 5766 6034 1632 Size: 922 1828 1384 268 1917 Fragments arranged by size: 1917 1828 1384 922 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 2428 2699 5218 6195 1203 Size: 1225 271 2519 977 1327 Fragments arranged by size: 2519 1327 1225 977 271 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 3140 1166 Size: 1974 4345 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AlwNI CAG_nnn'CTG Cuts at: 2813 3104 4062 5634 2813 Size: 291 958 1572 3498 Fragments arranged by size: 3498 1572 958 291 ApaLI G'TGCA_C Cuts at: 2193 4376 5532 2193 Size: 2183 1156 2980 Fragments arranged by size: 2980 2183 1156 ApoI r'AATT_y Cuts at: 1493 3628 3854 4006 6115 1493 Size: 2135 226 152 2109 1697 Fragments arranged by size: 2135 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2012 3644 1870 Size: 73 69 1632 4545 Fragments arranged by size: 4545 1632 73 69 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3640 5095 618 Size: 325 217 627 1853 1455 1842 Fragments arranged by size: 1853 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3638 1391 Size: 2247 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3237 928 Size: 971 1338 4010 Fragments arranged by size: 4010 1338 971 BbvCI CC'TCA_GC Cuts at: 3325 3325 Size: 6319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2903 2937 4510 4544 1903 Size: 34 966 34 1573 34 3678 Fragments arranged by size: 3678 1573 966 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4930 136 Size: 107 122 71 631 605 3258 1525 Fragments arranged by size: 3258 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 3736 3736 Size: 6319 Bme1580I G_kGCm'C Cuts at: 1790 2114 2197 2971 4380 5536 1790 Size: 324 83 774 1409 1156 2573 Fragments arranged by size: 2573 1409 1156 774 324 83 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3425 4050 5008 Size: 416 53 83 399 24 2008 625 958 Cuts at: 5008 442 Size: 1753 Fragments arranged by size: 2008 1753 958 625 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2909 2912 2909 Size: 3 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2245 3010 4979 1239 Size: 803 203 765 1969 2579 Fragments arranged by size: 2579 1969 803 765 203 Bpu10I CC'TnA_GC Cuts at: 3090 3325 3090 Size: 235 6084 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4444 5309 5607 4444 Size: 865 298 5156 Fragments arranged by size: 5156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4982 882 Size: 617 3483 2219 Fragments arranged by size: 3483 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4110 493 Size: 711 2906 2702 Fragments arranged by size: 2906 2702 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4126 4508 5143 275 Size: 53 83 186 936 2593 382 635 1451 Fragments arranged by size: 2593 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3262 4752 5150 5424 5571 5982 3262 Size: 1490 398 274 147 411 3599 Fragments arranged by size: 3599 1490 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 1178 Size: 30 165 30 1035 30 5029 Fragments arranged by size: 5029 1035 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2885 2901 3612 1186 Size: 1699 16 711 3893 Fragments arranged by size: 3893 1699 711 16 BseYI C'CCAG_C Cuts at: 3339 5522 6018 3339 Size: 2183 496 3640 Fragments arranged by size: 3640 2183 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3584 1163 Size: 573 1848 3898 Fragments arranged by size: 3898 1848 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3769 4530 5558 1540 Size: 275 186 1768 761 1028 2301 Fragments arranged by size: 2301 1768 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2197 4380 4465 5536 2197 Size: 2183 85 1071 2980 Fragments arranged by size: 2980 2183 1071 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2197 2971 3638 4380 4465 5536 Size: 399 324 83 774 667 742 85 1071 Cuts at: 5536 1391 Size: 2174 Fragments arranged by size: 2174 1071 774 742 667 399 324 85 83 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2798 3674 844 Size: 1954 876 3489 Fragments arranged by size: 3489 1954 876 BsrBI CCG'CTC Cuts at: 1394 1823 4206 1394 Size: 429 2383 3507 Fragments arranged by size: 3507 2383 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2175 3213 4814 4988 65 Size: 1183 927 1038 1601 174 1396 Fragments arranged by size: 1601 1396 1183 1038 927 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2440 3459 4963 5150 1700 Size: 110 630 1019 1504 187 2869 Fragments arranged by size: 2869 1504 1019 630 187 110 BsrGI T'GTAC_A Cuts at: 96 2832 96 Size: 2736 3583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 2246 2355 2375 4379 5391 2246 Size: 109 20 2004 1012 3174 Fragments arranged by size: 3174 2004 1012 109 20 BstAPI GCAn_nnn'nTGC Cuts at: 794 2737 2813 794 Size: 1943 76 4300 Fragments arranged by size: 4300 1943 76 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstXI CCAn_nnnn'nTGG Cuts at: 2953 2953 Size: 6319 BstYI r'GATC_y Cuts at: 1972 2554 3649 4016 4401 4418 5870 5956 5968 Size: 582 1095 367 385 17 1452 86 12 Cuts at: 5968 6314 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1095 582 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3222 3222 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 2762 2875 513 Size: 552 1697 113 3957 Fragments arranged by size: 3957 1697 552 113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 2114 3175 3835 4629 4649 2114 Size: 1061 660 794 20 3784 Fragments arranged by size: 3784 1061 794 660 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3502 3623 3502 Size: 121 6198 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2140 3766 4655 Size: 54 1425 50 275 18 310 1626 889 Cuts at: 4655 6284 8 Size: 1629 43 Fragments arranged by size: 1629 1626 1425 889 310 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2701 2840 3171 3308 4249 1353 Size: 1348 139 331 137 941 3423 Fragments arranged by size: 3423 1348 941 331 139 137 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3584 4906 5280 5426 387 Size: 1064 138 1995 1322 374 146 1280 Fragments arranged by size: 1995 1322 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2245 2554 3010 4382 4979 5766 Size: 393 410 203 309 456 1372 597 787 Cuts at: 5766 6034 1239 Size: 268 1524 Fragments arranged by size: 1524 1372 787 597 456 410 393 309 268 203 EcoNI CCTnn'n_nnAGG Cuts at: 2101 3540 2101 Size: 1439 4880 EcoO109I rG'GnC_Cy Cuts at: 2757 3336 2757 Size: 579 5740 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 FseI GG_CCGG'CC Cuts at: 3463 3463 Size: 6319 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 5466 1678 Size: 3788 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2438 2470 3422 3454 3588 Size: 32 135 32 866 32 952 32 134 Cuts at: 3588 3620 4960 4992 5034 5066 1373 Size: 32 1340 32 42 32 2626 Fragments arranged by size: 2626 1340 952 866 135 134 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2409 3663 3909 669 Size: 988 281 471 1254 246 3079 Fragments arranged by size: 3079 1254 988 471 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 2613 3918 2613 Size: 1305 5014 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4497 Fragments arranged by size: 4497 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3140 4277 4636 4795 518 Size: 648 1974 1137 359 159 2042 Fragments arranged by size: 2042 1974 1137 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3735 4412 5560 5805 1606 Size: 2129 677 1148 245 2120 Fragments arranged by size: 2129 2120 1148 677 245 NaeI GCC'GGC Cuts at: 3461 3461 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2762 2875 513 Size: 552 1697 113 3957 Fragments arranged by size: 3957 1697 552 113 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 3459 3459 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2432 2703 2828 3677 5222 1132 Size: 97 1203 271 125 849 1545 2229 Fragments arranged by size: 2229 1545 1203 849 271 125 97 PacI TTA_AT'TAA Cuts at: 2649 2649 Size: 6319 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 2428 2699 5218 2428 Size: 271 2519 3529 Fragments arranged by size: 3529 2519 271 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6319 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2757 3336 2757 Size: 579 5740 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspXI vC'TCGA_Gb Cuts at: 2012 2012 Size: 6319 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2578 2610 2578 Size: 32 6287 PstI C_TGCA'G Cuts at: 830 3115 3671 830 Size: 2285 556 3478 Fragments arranged by size: 3478 2285 556 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SanDI GG'GwC_CC Cuts at: 3336 3336 Size: 6319 SapI GCTCTTCn'nnn_ Cuts at: 2840 3171 2840 Size: 331 5988 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SexAI A'CCwGG_T Cuts at: 2859 2859 Size: 6319 SfcI C'TryA_G Cuts at: 826 1045 3111 3163 3667 4802 5483 5674 826 Size: 219 2066 52 504 1135 681 191 1471 Fragments arranged by size: 2066 1471 1135 681 504 219 191 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 3646 1872 Size: 1774 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2012 4423 5324 5586 820 Size: 197 926 69 2411 901 262 1553 Fragments arranged by size: 2411 1553 926 901 262 197 69 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 2828 3677 1132 Size: 1696 849 3774 Fragments arranged by size: 3774 1696 849 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2762 2875 3758 513 Size: 552 1697 113 883 3074 Fragments arranged by size: 3074 1697 883 552 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2344 3407 4346 4363 4516 4701 5146 Size: 1255 764 1063 939 17 153 185 445 Cuts at: 5146 325 Size: 1498 Fragments arranged by size: 1498 1255 1063 939 764 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 4565 6277 Size: 274 80 33 51 1443 855 1733 1712 Cuts at: 6277 96 Size: 138 Fragments arranged by size: 1733 1712 1443 855 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 2012 1943 Size: 69 6250 XmaI C'CCGG_G Cuts at: 1870 3644 1870 Size: 1774 4545 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PfoI PmlI PspOMI PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI