Restriction map for
pFN21AB9771
(Linear) (Six-base) MAPSORT of: KIBB9771.seq Check: 7082 from: 1 to: 1614
KIBB9771.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AclI AA'CG_TT
Cuts at: 0 461 1614
Size: 461 1153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 1614
Size: 549 1065
AflIII A'CryG_T
Cuts at: 0 423 694 1614
Size: 423 271 920
Fragments arranged by size:
920 423 271
AleI CACnn'nnGTG
Cuts at: 0 1135 1614
Size: 1135 479
AlwNI CAG_nnn'CTG
Cuts at: 0 808 1099 1614
Size: 808 291 515
Fragments arranged by size:
808 515 291
ApaLI G'TGCA_C
Cuts at: 0 188 1614
Size: 188 1426
AvaI C'yCGr_G
Cuts at: 0 7 1614
Size: 7 1607
BbsI GAAGACnn'nnnn_
Cuts at: 0 1232 1614
Size: 1232 382
BbvCI CC'TCA_GC
Cuts at: 0 1320 1614
Size: 1320 294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 898 932 1614
Size: 898 34 682
Fragments arranged by size:
898 682 34
Bme1580I G_kGCm'C
Cuts at: 0 109 192 966 1614
Size: 109 83 774 648
Fragments arranged by size:
774 648 109 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 1420 1614
Size: 1420 194
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 904 907 1614
Size: 904 3 707
Fragments arranged by size:
904 707 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 240 1005 1614
Size: 37 203 765 609
Fragments arranged by size:
765 609 203 37
Bpu10I CC'TnA_GC
Cuts at: 0 1085 1320 1614
Size: 1085 235 294
Fragments arranged by size:
1085 294 235
BsaWI w'CCGG_w
Cuts at: 0 1257 1614
Size: 1257 357
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 1614
Size: 433 30 1151
Fragments arranged by size:
1151 433 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 880 896 1607 1614
Size: 880 16 711 7
Fragments arranged by size:
880 711 16 7
BseYI C'CCAG_C
Cuts at: 0 1334 1614
Size: 1334 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1579 1614
Size: 1579 35
BsiHKAI G_wGCw'C
Cuts at: 0 192 1614
Size: 192 1422
Bsp1286I G_dGCh'C
Cuts at: 0 109 192 966 1614
Size: 109 83 774 648
Fragments arranged by size:
774 648 109 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 793 1614
Size: 793 821
BsrDI GCAATG_nn'
Cuts at: 0 170 1208 1614
Size: 170 1038 406
Fragments arranged by size:
1038 406 170
BsrFI r'CCGG_y
Cuts at: 0 435 1454 1614
Size: 435 1019 160
Fragments arranged by size:
1019 435 160
BsrGI T'GTAC_A
Cuts at: 0 827 1614
Size: 827 787
BssSI C'ACGA_G
Cuts at: 0 241 350 370 1614
Size: 241 109 20 1244
Fragments arranged by size:
1244 241 109 20
BstAPI GCAn_nnn'nTGC
Cuts at: 0 732 808 1614
Size: 732 76 806
Fragments arranged by size:
806 732 76
BstXI CCAn_nnnn'nTGG
Cuts at: 0 948 1614
Size: 948 666
BstYI r'GATC_y
Cuts at: 0 549 1614
Size: 549 1065
Bsu36I CC'TnA_GG
Cuts at: 0 1217 1614
Size: 1217 397
BtgI C'CryG_G
Cuts at: 0 757 870 1614
Size: 757 113 744
Fragments arranged by size:
757 744 113
BtsI GCAGTG_nn'
Cuts at: 0 109 1170 1614
Size: 109 1061 444
Fragments arranged by size:
1061 444 109
DraI TTT'AAA
Cuts at: 0 1497 1614
Size: 1497 117
EaeI y'GGCC_r
Cuts at: 0 135 1614
Size: 135 1479
EarI CTCTTCn'nnn_
Cuts at: 0 696 835 1166 1303 1614
Size: 696 139 331 137 311
Fragments arranged by size:
696 331 311 139 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1579 1614
Size: 1579 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 240 549 1005 1614
Size: 37 203 309 456 609
Fragments arranged by size:
609 456 309 203 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 96 1535 1614
Size: 96 1439 79
Fragments arranged by size:
1439 96 79
EcoO109I rG'GnC_Cy
Cuts at: 0 752 1331 1614
Size: 752 579 283
Fragments arranged by size:
752 579 283
FseI GG_CCGG'CC
Cuts at: 0 1458 1614
Size: 1458 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 1417 1449 1583 1614
Size: 433 32 952 32 134 31
Fragments arranged by size:
952 433 134 32 32 31
HincII GTy'rAC
Cuts at: 0 404 1614
Size: 404 1210
MfeI C'AATT_G
Cuts at: 0 608 1614
Size: 608 1006
MslI CAynn'nnrTG
Cuts at: 0 1135 1614
Size: 1135 479
NaeI GCC'GGC
Cuts at: 0 1456 1614
Size: 1456 158
NcoI C'CATG_G
Cuts at: 0 757 870 1614
Size: 757 113 744
Fragments arranged by size:
757 744 113
NgoMIV G'CCGG_C
Cuts at: 0 1454 1614
Size: 1454 160
NspI r_CATG'y
Cuts at: 0 427 698 823 1614
Size: 427 271 125 791
Fragments arranged by size:
791 427 271 125
PacI TTA_AT'TAA
Cuts at: 0 644 1614
Size: 644 970
PciI A'CATG_T
Cuts at: 0 423 694 1614
Size: 423 271 920
Fragments arranged by size:
920 423 271
PpuMI rG'GwC_Cy
Cuts at: 0 752 1331 1614
Size: 752 579 283
Fragments arranged by size:
752 579 283
PspXI vC'TCGA_Gb
Cuts at: 0 7 1614
Size: 7 1607
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 573 605 1614
Size: 573 32 1009
Fragments arranged by size:
1009 573 32
PstI C_TGCA'G
Cuts at: 0 1110 1614
Size: 1110 504
SanDI GG'GwC_CC
Cuts at: 0 1331 1614
Size: 1331 283
SapI GCTCTTCn'nnn_
Cuts at: 0 835 1166 1614
Size: 835 331 448
Fragments arranged by size:
835 448 331
SexAI A'CCwGG_T
Cuts at: 0 854 1614
Size: 854 760
SfcI C'TryA_G
Cuts at: 0 1106 1158 1614
Size: 1106 52 456
Fragments arranged by size:
1106 456 52
SmlI C'TyrA_G
Cuts at: 0 7 1614
Size: 7 1607
SphI G_CATG'C
Cuts at: 0 823 1614
Size: 823 791
StyI C'CwwG_G
Cuts at: 0 757 870 1614
Size: 757 113 744
Fragments arranged by size:
757 744 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 339 1402 1614
Size: 339 1063 212
Fragments arranged by size:
1063 339 212
TatI w'GTAC_w
Cuts at: 0 827 1614
Size: 827 787
XhoI C'TCGA_G
Cuts at: 0 7 1614
Size: 7 1607
Enzymes that do cut:
AclI AcuI AflIII AleI AlwNI ApaLI AvaI BbsI
BbvCI BcgI Bme1580I BmrI BplI BpmI Bpu10I BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FseI Hin4I HincII MfeI MslI NaeI NcoI NgoMIV
NspI PacI PciI PpuMI PspXI PsrI PstI SanDI
SapI SexAI SfcI SmlI SphI StyI TaqII TatI
XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI
MscI MspA1I NarI NdeI NheI NotI NruI NsiI
PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PvuI PvuII RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI