(Circular) (Six-base) MAPSORT of: pFN21AB5694.seq Check: 839 from: 1 to: 6643 pFN21AB5694 6643 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3042 4453 278 Size: 53 83 186 936 1506 1411 2468 Fragments arranged by size: 2468 1506 1411 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2184 2400 3986 1937 Size: 247 216 1586 4594 Fragments arranged by size: 4594 1586 247 216 Acc65I G'GTAC_C Cuts at: 2502 3964 2502 Size: 1462 5181 AclI AA'CG_TT Cuts at: 1563 4770 5143 1563 Size: 3207 373 3063 Fragments arranged by size: 3207 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 2567 2648 2810 2833 3164 4706 6090 Size: 530 405 81 162 23 331 1542 1384 Cuts at: 6090 6358 1632 Size: 268 1917 Fragments arranged by size: 1917 1542 1384 530 405 331 268 162 81 23 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6446 AflIII A'CryG_T Cuts at: 1203 5542 6519 1203 Size: 4339 977 1327 Fragments arranged by size: 4339 1327 977 AgeI A'CCGG_T Cuts at: 5474 5474 Size: 6643 AhdI GACnn_n'nnGTC Cuts at: 5372 5372 Size: 6643 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6643 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6643 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3110 3142 3110 Size: 32 6611 AlwNI CAG_nnn'CTG Cuts at: 2971 3062 3136 3160 4386 5958 2971 Size: 91 74 24 1226 1572 3656 Fragments arranged by size: 3656 1572 1226 91 74 24 ApaLI G'TGCA_C Cuts at: 4700 5856 4700 Size: 1156 5487 ApoI r'AATT_y Cuts at: 1493 3952 4178 4330 6439 1493 Size: 2459 226 152 2109 1697 Fragments arranged by size: 2459 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5197 160 Size: 872 4165 1606 Fragments arranged by size: 4165 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 AvaI C'yCGr_G Cuts at: 1870 1943 3122 3968 1870 Size: 73 1179 846 4545 Fragments arranged by size: 4545 1179 846 73 BamHI G'GATC_C Cuts at: 3973 4340 3973 Size: 367 6276 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2502 3372 3964 5419 618 Size: 325 217 627 715 870 592 1455 1842 Fragments arranged by size: 1842 1455 870 715 627 592 325 217 BanII G_rGCy'C Cuts at: 1391 2679 2778 3904 3962 1391 Size: 1288 99 1126 58 4072 Fragments arranged by size: 4072 1288 1126 99 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2655 928 Size: 971 756 4916 Fragments arranged by size: 4916 971 756 BbvCI CC'TCA_GC Cuts at: 2174 3599 2174 Size: 1425 5218 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4834 4868 1903 Size: 34 2897 34 3678 Fragments arranged by size: 3678 2897 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4535 5745 4535 Size: 1210 5433 BclI T'GATC_A Cuts at: 1548 1548 Size: 6643 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3775 5254 136 Size: 107 122 71 631 605 2103 1479 1525 Fragments arranged by size: 2103 1525 1479 631 605 122 107 71 BglII A'GATC_T Cuts at: 6638 6638 Size: 6643 BlpI GC'TnA_GC Cuts at: 2088 2279 4060 2088 Size: 191 1781 4671 Fragments arranged by size: 4671 1781 191 Bme1580I G_kGCm'C Cuts at: 1790 2079 3240 3375 4704 5860 1790 Size: 289 1161 135 1329 1156 2573 Fragments arranged by size: 2573 1329 1161 1156 289 135 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6643 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2152 2656 2688 Size: 416 53 83 399 24 735 504 32 Cuts at: 2688 4374 5332 442 Size: 1686 958 1753 Fragments arranged by size: 1753 1686 958 735 504 416 399 83 53 32 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6643 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2945 3566 5303 1239 Size: 1031 675 621 1737 2579 Fragments arranged by size: 2579 1737 1031 675 621 Bpu10I CC'TnA_GC Cuts at: 2103 2174 3446 3599 3781 2103 Size: 71 1272 153 182 4965 Fragments arranged by size: 4965 1272 182 153 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2301 2499 4768 5633 5931 2301 Size: 198 2269 865 298 3013 Fragments arranged by size: 3013 2269 865 298 198 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3903 5306 882 Size: 617 2404 1403 2219 Fragments arranged by size: 2404 2219 1403 617 BsaAI yAC'GTr Cuts at: 493 1204 4434 493 Size: 711 3230 2702 Fragments arranged by size: 3230 2702 711 BsaBI GATnn'nnATC Cuts at: 6637 6637 Size: 6643 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3039 3230 4450 4832 Size: 53 83 186 936 1506 191 1220 382 Cuts at: 4832 5467 275 Size: 635 1451 Fragments arranged by size: 1506 1451 1220 936 635 382 191 186 83 53 BsaWI w'CCGG_w Cuts at: 2410 5076 5474 5748 5895 6306 2410 Size: 2666 398 274 147 411 2747 Fragments arranged by size: 2747 2666 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3626 3656 1178 Size: 30 165 30 2223 30 4165 Fragments arranged by size: 4165 2223 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2429 2486 2567 1186 Size: 1243 57 81 5262 Fragments arranged by size: 5262 1243 81 57 BseYI C'CCAG_C Cuts at: 2870 3239 3727 5846 6342 2870 Size: 369 488 2119 496 3171 Fragments arranged by size: 3171 2119 496 488 369 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3793 1163 Size: 573 2057 4013 Fragments arranged by size: 4013 2057 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4093 4854 5882 1540 Size: 275 186 2092 761 1028 2301 Fragments arranged by size: 2301 2092 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2679 3452 4704 4789 5860 2679 Size: 773 1252 85 1071 3462 Fragments arranged by size: 3462 1252 1071 773 85 BsmI GAATG_Cn' Cuts at: 2790 3305 3503 4154 4247 2790 Size: 515 198 651 93 5186 Fragments arranged by size: 5186 651 515 198 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3816 3816 Size: 6643 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2679 2778 3240 3375 3452 3904 Size: 399 289 600 99 462 135 77 452 Cuts at: 3904 3962 4704 4789 5860 1391 Size: 58 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 600 462 452 399 289 135 99 85 77 58 BspEI T'CCGG_A Cuts at: 2410 6306 2410 Size: 3896 2747 BspHI T'CATG_A Cuts at: 4532 6262 4532 Size: 1730 4913 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3998 844 Size: 3154 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4530 1394 Size: 429 2707 3507 Fragments arranged by size: 3507 2707 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5138 5312 65 Size: 1183 3890 174 1396 Fragments arranged by size: 3890 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2538 2685 5287 5474 1700 Size: 110 728 147 2602 187 2869 Fragments arranged by size: 2869 2602 728 187 147 110 BsrGI T'GTAC_A Cuts at: 96 2607 96 Size: 2511 4132 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6643 BssSI C'ACGA_G Cuts at: 3464 4703 5715 3464 Size: 1239 1012 4392 Fragments arranged by size: 4392 1239 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6643 BstBI TT'CG_AA Cuts at: 5447 5447 Size: 6643 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6643 BstXI CCAn_nnnn'nTGG Cuts at: 3517 3517 Size: 6643 BstYI r'GATC_y Cuts at: 1972 3430 3973 4340 4725 4742 6194 6280 6292 Size: 1458 543 367 385 17 1452 86 12 Cuts at: 6292 6638 1972 Size: 346 1977 Fragments arranged by size: 1977 1458 1452 543 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2469 2469 Size: 6643 BtgI C'CryG_G Cuts at: 513 1065 2004 2778 513 Size: 552 939 774 4378 Fragments arranged by size: 4378 939 774 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5904 Fragments arranged by size: 5904 683 56 BtsI GCAGTG_nn' Cuts at: 3609 4159 4953 4973 3609 Size: 550 794 20 5279 Fragments arranged by size: 5279 794 550 20 ClaI AT'CG_AT Cuts at: 1554 3930 1554 Size: 2376 4267 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6608 DraI TTT'AAA Cuts at: 3947 3947 Size: 6643 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2593 2912 5650 809 Size: 507 1277 319 2738 1802 Fragments arranged by size: 2738 1802 1277 507 319 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2536 3279 4090 Size: 54 1425 50 275 18 706 743 811 Cuts at: 4090 4979 6608 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 811 743 706 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4090 1537 Size: 275 2278 4090 Fragments arranged by size: 4090 2278 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2886 3138 3545 3855 4573 1353 Size: 1533 252 407 310 718 3423 Fragments arranged by size: 3423 1533 718 407 310 252 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5230 5604 5750 387 Size: 1064 138 3641 374 146 1280 Fragments arranged by size: 3641 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2677 2677 Size: 6643 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2270 2567 2648 2810 2833 2945 Size: 393 530 108 297 81 162 23 112 Cuts at: 2945 3164 3566 4706 5303 6090 6358 1239 Size: 219 402 1140 597 787 268 1524 Fragments arranged by size: 1524 1140 787 597 530 402 393 297 268 219 162 112 108 81 23 EcoNI CCTnn'n_nnAGG Cuts at: 3875 3875 Size: 6643 EcoO109I rG'GnC_Cy Cuts at: 2914 2968 3118 3170 2914 Size: 54 150 52 6387 Fragments arranged by size: 6387 150 54 52 EcoRI G'AATT_C Cuts at: 3952 4330 3952 Size: 378 6265 EcoRV GAT'ATC Cuts at: 2991 2991 Size: 6643 FseI GG_CCGG'CC Cuts at: 2542 2542 Size: 6643 FspI TGC'GCA Cuts at: 5149 5149 Size: 6643 HaeII r_GCGC'y Cuts at: 1678 2365 2956 3735 3781 5790 1678 Size: 687 591 779 46 2009 2531 Fragments arranged by size: 2531 2009 779 687 591 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5284 5316 5358 5390 1373 Size: 32 135 32 3712 32 42 32 2626 Fragments arranged by size: 3712 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2185 2434 3274 3403 3987 4233 Size: 988 281 247 249 840 129 584 246 Cuts at: 4233 669 Size: 3079 Fragments arranged by size: 3079 988 840 584 281 249 247 246 129 HindIII A'AGCT_T Cuts at: 748 748 Size: 6643 HpaI GTT'AAC Cuts at: 4233 4233 Size: 6643 KpnI G_GTAC'C Cuts at: 2506 3968 2506 Size: 1462 5181 MfeI C'AATT_G Cuts at: 4242 4242 Size: 6643 MluI A'CGCG_T Cuts at: 6519 6519 Size: 6643 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3462 3534 3606 5757 5941 1305 Size: 2157 72 72 2151 184 2007 Fragments arranged by size: 2157 2151 2007 184 72 72 MscI TGG'CCA Cuts at: 10 64 1489 1832 3281 10 Size: 54 1425 343 1449 3372 Fragments arranged by size: 3372 1449 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2450 4601 4960 5119 518 Size: 648 1284 2151 359 159 2042 Fragments arranged by size: 2151 2042 1284 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2275 2638 3268 3888 4059 4736 5884 6129 Size: 669 363 630 620 171 677 1148 245 Cuts at: 6129 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 669 630 620 363 245 171 NaeI GCC'GGC Cuts at: 2540 2540 Size: 6643 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5152 Fragments arranged by size: 5152 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6643 NgoMIV G'CCGG_C Cuts at: 2538 2538 Size: 6643 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6643 NotI GC'GGCC_GC Cuts at: 4090 4090 Size: 6643 NspI r_CATG'y Cuts at: 1132 1229 3944 4001 5546 1132 Size: 97 2715 57 1545 2229 Fragments arranged by size: 2715 2229 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 2440 1348 Size: 1092 5551 PciI A'CATG_T Cuts at: 5542 5542 Size: 6643 PflMI CCAn_nnn'nTGG Cuts at: 1108 2590 3160 3393 1108 Size: 1482 570 233 4358 Fragments arranged by size: 4358 1482 570 233 PfoI T'CCnGG_A Cuts at: 2286 3392 3543 3904 2286 Size: 1106 151 361 5025 Fragments arranged by size: 5025 1106 361 151 PmeI GTTT'AAAC Cuts at: 3947 3947 Size: 6643 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4712 4744 6225 6257 4712 Size: 32 1481 32 5098 Fragments arranged by size: 5098 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2914 3118 2914 Size: 204 6439 PshAI GACnn'nnGTC Cuts at: 1613 2318 1613 Size: 705 5938 PsiI TTA'TAA Cuts at: 4213 4213 Size: 6643 PspXI vC'TCGA_Gb Cuts at: 3122 3122 Size: 6643 PstI C_TGCA'G Cuts at: 830 3879 3995 830 Size: 3049 116 3478 Fragments arranged by size: 3478 3049 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6643 PvuII CAG'CTG Cuts at: 2638 3268 2638 Size: 630 6013 SacI G_AGCT'C Cuts at: 2679 2679 Size: 6643 SalI G'TCGA_C Cuts at: 1936 2183 3985 1936 Size: 247 1802 4594 Fragments arranged by size: 4594 1802 247 SbfI CC_TGCA'GG Cuts at: 3879 3995 3879 Size: 116 6527 ScaI AGT'ACT Cuts at: 4891 6603 4891 Size: 1712 4931 SfcI C'TryA_G Cuts at: 826 1045 3875 3991 5126 5807 5998 826 Size: 219 2830 116 1135 681 191 1471 Fragments arranged by size: 2830 1471 1135 681 219 191 116 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6643 SmaI CCC'GGG Cuts at: 1872 3970 1872 Size: 2098 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2316 2514 3122 4747 5648 5910 Size: 197 926 373 198 608 1625 901 262 Cuts at: 5910 820 Size: 1553 Fragments arranged by size: 1625 1553 926 901 608 373 262 198 197 SnaBI TAC'GTA Cuts at: 493 4434 493 Size: 3941 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6643 SphI G_CATG'C Cuts at: 1132 4001 1132 Size: 2869 3774 SspI AAT'ATT Cuts at: 5 52 4567 5 Size: 47 4515 2081 Fragments arranged by size: 4515 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6643 StyI C'CwwG_G Cuts at: 513 1065 2004 2164 3296 4082 513 Size: 552 939 160 1132 786 3074 Fragments arranged by size: 3074 1132 939 786 552 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2358 3316 4670 4687 4840 5025 5470 Size: 1255 778 958 1354 17 153 185 445 Cuts at: 5470 325 Size: 1498 Fragments arranged by size: 1498 1354 1255 958 778 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2607 4889 6601 Size: 274 80 33 51 1443 630 2282 1712 Cuts at: 6601 96 Size: 138 Fragments arranged by size: 2282 1712 1443 630 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2801 2833 3426 3458 3601 3633 6603 6635 2801 Size: 32 593 32 143 32 2970 32 2809 Fragments arranged by size: 2970 2809 593 143 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6643 XbaI T'CTAG_A Cuts at: 3979 3979 Size: 6643 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2255 2255 Size: 6643 XhoI C'TCGA_G Cuts at: 1943 3122 1943 Size: 1179 5464 XmaI C'CCGG_G Cuts at: 1870 3968 1870 Size: 2098 4545 XmnI GAAnn'nnTTC Cuts at: 4772 4772 Size: 6643 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3040 4451 276 Size: 53 83 186 936 1506 1411 2468 Fragments arranged by size: 2468 1506 1411 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII FalI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI