(Circular) (Six-base) MAPSORT of: pFN21AB0465.seq Check: 9077 from: 1 to: 6139 pFN21AB0465 6139 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2199 3949 278 Size: 53 83 186 936 663 1750 2468 Fragments arranged by size: 2468 1750 936 663 186 83 53 AccI GT'mk_AC Cuts at: 1937 2925 3482 1937 Size: 988 557 4594 Fragments arranged by size: 4594 988 557 Acc65I G'GTAC_C Cuts at: 2259 3460 2259 Size: 1201 4938 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2441 4202 5586 5854 1632 Size: 809 1761 1384 268 1917 Fragments arranged by size: 1917 1761 1384 809 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 2412 5038 6015 1203 Size: 1209 2626 977 1327 Fragments arranged by size: 2626 1327 1209 977 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2963 1184 Size: 1779 4360 AlwNI CAG_nnn'CTG Cuts at: 2029 2242 2349 3268 3882 5454 2029 Size: 213 107 919 614 1572 2714 Fragments arranged by size: 2714 1572 919 614 213 107 ApaLI G'TGCA_C Cuts at: 2993 4196 5352 2993 Size: 1203 1156 3780 Fragments arranged by size: 3780 1203 1156 ApoI r'AATT_y Cuts at: 1493 3448 3674 3826 5935 1493 Size: 1955 226 152 2109 1697 Fragments arranged by size: 2109 1955 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 3196 3228 3321 3464 1870 Size: 73 1253 32 93 143 4545 Fragments arranged by size: 4545 1253 143 93 73 32 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2169 2202 2169 Size: 33 6106 BamHI G'GATC_C Cuts at: 3469 3836 3469 Size: 367 5772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2259 3460 4915 618 Size: 325 217 627 472 1201 1455 1842 Fragments arranged by size: 1842 1455 1201 627 472 325 217 BanII G_rGCy'C Cuts at: 1391 3197 3344 3458 1391 Size: 1806 147 114 4072 Fragments arranged by size: 4072 1806 147 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2602 2854 928 Size: 971 703 252 4213 Fragments arranged by size: 4213 971 703 252 BbvCI CC'TCA_GC Cuts at: 2108 2108 Size: 6139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4330 4364 1903 Size: 34 50 34 2309 34 3678 Fragments arranged by size: 3678 2309 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2197 4031 5241 2197 Size: 1834 1210 3095 Fragments arranged by size: 3095 1834 1210 BclI T'GATC_A Cuts at: 1548 2868 1548 Size: 1320 4819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3028 3242 4750 Size: 107 122 71 631 605 1356 214 1508 Cuts at: 4750 136 Size: 1525 Fragments arranged by size: 1525 1508 1356 631 605 214 122 107 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 3139 3556 3139 Size: 417 5722 Bme1580I G_kGCm'C Cuts at: 1790 2997 4200 5356 1790 Size: 1207 1203 1156 2573 Fragments arranged by size: 2573 1207 1203 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3870 4828 442 Size: 416 53 83 399 24 2453 958 1753 Fragments arranged by size: 2453 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3383 1056 Size: 2327 3812 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2842 3289 2842 Size: 447 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2188 2273 3068 4799 1239 Size: 949 85 795 1731 2579 Fragments arranged by size: 2579 1731 949 795 85 Bpu10I CC'TnA_GC Cuts at: 2108 2108 Size: 6139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4264 5129 5427 4264 Size: 865 298 4976 Fragments arranged by size: 4976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2830 2963 4802 882 Size: 617 1331 133 1839 2219 Fragments arranged by size: 2219 1839 1331 617 133 BsaAI yAC'GTr Cuts at: 493 1204 2413 3930 493 Size: 711 1209 1517 2702 Fragments arranged by size: 2702 1517 1209 711 BsaBI GATnn'nnATC Cuts at: 6133 6133 Size: 6139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2196 3946 4328 4963 Size: 53 83 186 936 663 1750 382 635 Cuts at: 4963 275 Size: 1451 Fragments arranged by size: 1750 1451 936 663 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4572 4970 5244 5391 5802 4572 Size: 398 274 147 411 4909 Fragments arranged by size: 4909 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5914 Fragments arranged by size: 5914 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2092 2834 2901 1186 Size: 847 59 742 67 4424 Fragments arranged by size: 4424 847 742 67 59 BseYI C'CCAG_C Cuts at: 2638 2759 3103 5342 5838 2638 Size: 121 344 2239 496 2939 Fragments arranged by size: 2939 2239 496 344 121 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2525 1163 Size: 573 789 4777 Fragments arranged by size: 4777 789 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3589 4350 5378 1540 Size: 275 186 1588 761 1028 2301 Fragments arranged by size: 2301 1588 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2997 4200 4285 5356 2997 Size: 1203 85 1071 3780 Fragments arranged by size: 3780 1203 1071 85 BsiWI C'GTAC_G Cuts at: 2409 2409 Size: 6139 BsmI GAATG_Cn' Cuts at: 3650 3743 3650 Size: 93 6046 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2997 3197 3344 3458 4200 4285 5356 Size: 399 1207 200 147 114 742 85 1071 Cuts at: 5356 1391 Size: 2174 Fragments arranged by size: 2174 1207 1071 742 399 200 147 114 85 BspEI T'CCGG_A Cuts at: 5802 5802 Size: 6139 BspHI T'CATG_A Cuts at: 2613 4028 5758 2613 Size: 1415 1730 2994 Fragments arranged by size: 2994 1730 1415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2808 3015 3384 3494 844 Size: 1964 207 369 110 3489 Fragments arranged by size: 3489 1964 369 207 110 BsrBI CCG'CTC Cuts at: 1394 1823 2102 4026 1394 Size: 429 279 1924 3507 Fragments arranged by size: 3507 1924 429 279 BsrDI GCAATG_nn' Cuts at: 65 1248 4634 4808 65 Size: 1183 3386 174 1396 Fragments arranged by size: 3386 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2262 2721 2890 4783 4970 1700 Size: 110 452 459 169 1893 187 2869 Fragments arranged by size: 2869 1893 459 452 187 169 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 2029 3007 3019 794 Size: 1235 978 12 3914 Fragments arranged by size: 3914 1235 978 12 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 2056 2056 Size: 6139 BstYI r'GATC_y Cuts at: 1972 3306 3469 3836 4221 4238 5690 5776 5788 Size: 1334 163 367 385 17 1452 86 12 Cuts at: 5788 6134 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1334 385 367 346 163 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2301 3074 2301 Size: 773 5366 BtgI C'CryG_G Cuts at: 513 1065 2337 3144 513 Size: 552 1272 807 3508 Fragments arranged by size: 3508 1272 807 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5400 Fragments arranged by size: 5400 683 56 BtsI GCAGTG_nn' Cuts at: 2895 3154 3655 4449 4469 2895 Size: 259 501 794 20 4565 Fragments arranged by size: 4565 794 501 259 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2201 2606 5146 809 Size: 507 885 405 2540 1802 Fragments arranged by size: 2540 1802 885 507 405 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2892 2964 3586 Size: 54 1425 50 275 18 1062 72 622 Cuts at: 3586 4475 6104 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1062 889 622 275 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3586 1537 Size: 275 1774 4090 Fragments arranged by size: 4090 1774 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2738 3185 4069 1353 Size: 1385 447 884 3423 Fragments arranged by size: 3423 1385 884 447 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2708 4726 5100 5246 387 Size: 1064 138 1119 2018 374 146 1280 Fragments arranged by size: 2018 1280 1119 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2188 2273 2441 3068 4202 4799 5586 Size: 393 556 85 168 627 1134 597 787 Cuts at: 5586 5854 1239 Size: 268 1524 Fragments arranged by size: 1524 1134 787 627 597 556 393 268 168 85 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2060 Size: 6139 EcoO109I rG'GnC_Cy Cuts at: 2353 3099 3173 2353 Size: 746 74 5319 Fragments arranged by size: 5319 746 74 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 2030 2578 5286 1678 Size: 352 548 2708 2531 Fragments arranged by size: 2708 2531 548 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3122 3154 4780 4812 4854 Size: 32 135 32 1550 32 1626 32 42 Cuts at: 4854 4886 1373 Size: 32 2626 Fragments arranged by size: 2626 1626 1550 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3483 3729 669 Size: 988 281 1149 396 246 3079 Fragments arranged by size: 3079 1149 988 396 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KpnI G_GTAC'C Cuts at: 2263 3464 2263 Size: 1201 4938 MfeI C'AATT_G Cuts at: 3738 3738 Size: 6139 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 2622 5253 5437 1305 Size: 792 525 2631 184 2007 Fragments arranged by size: 2631 2007 792 525 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4317 Fragments arranged by size: 4317 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2777 4097 4456 4615 518 Size: 648 1611 1320 359 159 2042 Fragments arranged by size: 2042 1611 1320 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3555 4232 5380 5625 1606 Size: 1949 677 1148 245 2120 Fragments arranged by size: 2120 1949 1148 677 245 NaeI GCC'GGC Cuts at: 2723 2892 2723 Size: 169 5970 NcoI C'CATG_G Cuts at: 513 1065 3144 513 Size: 552 2079 3508 Fragments arranged by size: 3508 2079 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NgoMIV G'CCGG_C Cuts at: 2721 2890 2721 Size: 169 5970 NheI G'CTAG_C Cuts at: 1052 3379 1052 Size: 2327 3812 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 3497 5042 1132 Size: 97 2268 1545 2229 Fragments arranged by size: 2268 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 5038 5038 Size: 6139 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6139 PfoI T'CCnGG_A Cuts at: 2355 2550 3288 2355 Size: 195 738 5206 Fragments arranged by size: 5206 738 195 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2353 3173 2353 Size: 820 5319 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PstI C_TGCA'G Cuts at: 830 3012 3161 3264 3491 830 Size: 2182 149 103 227 3478 Fragments arranged by size: 3478 2182 227 149 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SanDI GG'GwC_CC Cuts at: 2353 2353 Size: 6139 SapI GCTCTTCn'nnn_ Cuts at: 3185 3185 Size: 6139 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 2270 4387 6099 2270 Size: 2117 1712 2310 Fragments arranged by size: 2310 2117 1712 SexAI A'CCwGG_T Cuts at: 3039 3039 Size: 6139 SfcI C'TryA_G Cuts at: 826 1045 2137 2473 3008 3157 3260 3394 3487 Size: 219 1092 336 535 149 103 134 93 Cuts at: 3487 4622 5303 5494 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1092 681 535 336 219 191 149 134 103 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 2721 1700 Size: 1021 5118 SmaI CCC'GGG Cuts at: 1872 3466 1872 Size: 1594 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4243 5144 5406 820 Size: 197 926 2300 901 262 1553 Fragments arranged by size: 2300 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3930 493 Size: 3437 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 3236 1517 Size: 1719 4420 StyI C'CwwG_G Cuts at: 513 1065 3144 3578 513 Size: 552 2079 434 3074 Fragments arranged by size: 3074 2079 552 434 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2848 4166 4183 4336 4521 4966 325 Size: 1255 1268 1318 17 153 185 445 1498 Fragments arranged by size: 1498 1318 1268 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2268 4385 6097 Size: 274 80 33 51 1443 291 2117 1712 Cuts at: 6097 96 Size: 138 Fragments arranged by size: 2117 1712 1443 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6099 6131 6099 Size: 32 6107 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6139 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2218 2218 Size: 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3464 1870 Size: 1594 4545 XmnI GAAnn'nnTTC Cuts at: 4268 4268 Size: 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2197 3947 276 Size: 53 83 186 936 663 1750 2468 Fragments arranged by size: 2468 1750 936 663 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI