(Circular) (Six-base) MAPSORT of: pFN21AB5389.seq Check: 4586 from: 1 to: 6142 pFN21AB5389 6142 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3952 278 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 2883 3441 4269 4642 1563 Size: 1320 558 828 373 3063 Fragments arranged by size: 3063 1320 828 558 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 2915 4205 5589 5857 1632 Size: 968 315 1290 1384 268 1917 Fragments arranged by size: 1917 1384 1290 968 315 268 AfeI AGC'GCT Cuts at: 3137 3137 Size: 6142 AflII C'TTAA_G Cuts at: 820 1017 2078 820 Size: 197 1061 4884 Fragments arranged by size: 4884 1061 197 AflIII A'CryG_T Cuts at: 1203 3177 5041 6018 1203 Size: 1974 1864 977 1327 Fragments arranged by size: 1974 1864 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 3176 1166 Size: 2010 4132 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2064 1184 Size: 880 5262 AlwNI CAG_nnn'CTG Cuts at: 2805 2815 3885 5457 2805 Size: 10 1070 1572 3490 Fragments arranged by size: 3490 1572 1070 10 ApaI G_GGCC'C Cuts at: 2026 2026 Size: 6142 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 2564 3451 3677 3829 5938 1493 Size: 1071 887 226 152 2109 1697 Fragments arranged by size: 2109 1697 1071 887 226 152 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 3467 1870 Size: 73 1524 4545 Fragments arranged by size: 4545 1524 73 BamHI G'GATC_C Cuts at: 2251 3472 3839 2251 Size: 1221 367 4554 Fragments arranged by size: 4554 1221 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2651 3463 4918 618 Size: 325 217 627 864 812 1455 1842 Fragments arranged by size: 1842 1455 864 812 627 325 217 BanII G_rGCy'C Cuts at: 1391 2026 2394 2526 3063 3461 1391 Size: 635 368 132 537 398 4072 Fragments arranged by size: 4072 635 537 398 368 132 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 2844 928 Size: 971 251 694 4226 Fragments arranged by size: 4226 971 694 251 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 2199 1548 Size: 651 5491 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3303 4753 136 Size: 107 122 71 631 605 1631 1450 1525 Fragments arranged by size: 1631 1525 1450 631 605 122 107 71 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 2388 2462 3559 2388 Size: 74 1097 4971 Fragments arranged by size: 4971 1097 74 Bme1580I G_kGCm'C Cuts at: 1790 2026 3187 4203 5359 1790 Size: 236 1161 1016 1156 2573 Fragments arranged by size: 2573 1161 1156 1016 236 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2173 2488 3873 Size: 416 53 83 399 24 756 315 1385 Cuts at: 3873 4831 442 Size: 958 1753 Fragments arranged by size: 1753 1385 958 756 416 399 315 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2588 1056 Size: 1532 4610 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4802 1239 Size: 3563 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2672 2991 4267 5132 5430 2672 Size: 319 1276 865 298 3384 Fragments arranged by size: 3384 1276 865 319 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2903 3260 4805 882 Size: 617 1404 357 1545 2219 Fragments arranged by size: 2219 1545 1404 617 357 BsaAI yAC'GTr Cuts at: 493 1204 3933 493 Size: 711 2729 2702 Fragments arranged by size: 2729 2702 711 BsaBI GATnn'nnATC Cuts at: 6136 6136 Size: 6142 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3949 4331 4966 275 Size: 53 83 186 936 2416 382 635 1451 Fragments arranged by size: 2416 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3279 4575 4973 5247 5394 5805 3279 Size: 1296 398 274 147 411 3616 Fragments arranged by size: 3616 1296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2298 2328 2663 2693 1178 Size: 30 165 30 895 30 335 30 4627 Fragments arranged by size: 4627 895 335 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6142 BseYI C'CCAG_C Cuts at: 5345 5841 5345 Size: 496 5646 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2965 3260 1163 Size: 573 1229 295 4045 Fragments arranged by size: 4045 1229 573 295 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2526 2681 3063 4203 4288 5359 2526 Size: 155 382 1140 85 1071 3309 Fragments arranged by size: 3309 1140 1071 382 155 85 BsmI GAATG_Cn' Cuts at: 3653 3746 3653 Size: 93 6049 BsmBI CGTCTCn'nnnn_ Cuts at: 2949 2949 Size: 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2026 2394 2526 2681 3063 3187 3461 Size: 399 236 368 132 155 382 124 274 Cuts at: 3461 4203 4288 5359 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 368 274 236 155 132 124 85 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 2742 4031 5761 2742 Size: 1289 1730 3123 Fragments arranged by size: 3123 1730 1289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2485 3282 3497 844 Size: 1641 797 215 3489 Fragments arranged by size: 3489 1641 797 215 BsrBI CCG'CTC Cuts at: 1394 1823 4029 1394 Size: 429 2206 3507 Fragments arranged by size: 3507 2206 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2369 3000 4637 4811 65 Size: 1183 1121 631 1637 174 1396 Fragments arranged by size: 1637 1396 1183 1121 631 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4786 4973 1700 Size: 110 2976 187 2869 Fragments arranged by size: 2976 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 3166 1184 Size: 1982 4160 BstYI r'GATC_y Cuts at: 1972 2251 3472 3839 4224 4241 5693 5779 5791 Size: 279 1221 367 385 17 1452 86 12 Cuts at: 5791 6137 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 279 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2331 3187 3237 513 Size: 552 939 327 856 50 3418 Fragments arranged by size: 3418 939 856 552 327 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 3132 3327 3658 4452 4472 3132 Size: 195 331 794 20 4802 Fragments arranged by size: 4802 794 331 195 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2214 2249 529 Size: 35 1650 35 4422 Fragments arranged by size: 4422 1650 35 35 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DraIII CAC_nnn'GTG Cuts at: 3082 3082 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2454 3589 Size: 54 1425 50 275 18 177 447 1135 Cuts at: 3589 4478 6107 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1135 889 447 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2079 2151 2283 2901 4072 1353 Size: 726 72 132 618 1171 3423 Fragments arranged by size: 3423 1171 726 618 132 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2815 4729 5103 5249 387 Size: 1064 138 1226 1914 374 146 1280 Fragments arranged by size: 1914 1280 1226 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2524 3061 2524 Size: 537 5605 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2600 2915 4205 4802 5589 5857 1239 Size: 393 968 315 1290 597 787 268 1524 Fragments arranged by size: 1524 1290 968 787 597 393 315 268 EcoO109I rG'GnC_Cy Cuts at: 2023 3313 2023 Size: 1290 4852 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 3139 5289 1678 Size: 1461 2150 2531 Fragments arranged by size: 2531 2150 1461 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 2663 2695 2737 Size: 32 135 32 671 32 388 32 42 Cuts at: 2737 2769 4783 4815 4857 4889 1373 Size: 32 2014 32 42 32 2626 Fragments arranged by size: 2626 2014 671 388 135 42 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3486 3732 669 Size: 988 281 1548 246 3079 Fragments arranged by size: 3079 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5256 5440 1305 Size: 3951 184 2007 Fragments arranged by size: 3951 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2456 10 Size: 54 1425 343 177 447 3696 Fragments arranged by size: 3696 1425 447 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3176 4100 4459 4618 518 Size: 648 2010 924 359 159 2042 Fragments arranged by size: 2042 2010 924 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2248 2441 3189 3558 4235 5383 5628 1606 Size: 642 193 748 369 677 1148 245 2120 Fragments arranged by size: 2120 1148 748 677 642 369 245 193 NcoI C'CATG_G Cuts at: 513 1065 2004 2331 513 Size: 552 939 327 4324 Fragments arranged by size: 4324 939 552 327 NdeI CA'TA_TG Cuts at: 387 387 Size: 6142 NheI G'CTAG_C Cuts at: 1052 2584 1052 Size: 1532 4610 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 3500 5045 1132 Size: 97 2271 1545 2229 Fragments arranged by size: 2271 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2657 1348 Size: 1309 4833 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2866 3082 1108 Size: 1758 216 4168 Fragments arranged by size: 4168 1758 216 PfoI T'CCnGG_A Cuts at: 3369 3369 Size: 6142 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PspOMI G'GGCC_C Cuts at: 2022 2022 Size: 6142 PstI C_TGCA'G Cuts at: 830 2247 3127 3494 830 Size: 1417 880 367 3478 Fragments arranged by size: 3478 1417 880 367 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2441 2441 Size: 6142 SacI G_AGCT'C Cuts at: 2526 3063 2526 Size: 537 5605 SacII CC_GC'GG Cuts at: 3190 3190 Size: 6142 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SapI GCTCTTCn'nnn_ Cuts at: 2283 2283 Size: 6142 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 2933 4390 6102 2933 Size: 1457 1712 2973 Fragments arranged by size: 2973 1712 1457 SexAI A'CCwGG_T Cuts at: 3078 3078 Size: 6142 SfcI C'TryA_G Cuts at: 826 1045 2243 3123 3490 4625 5306 5497 826 Size: 219 1198 880 367 1135 681 191 1471 Fragments arranged by size: 1471 1198 1135 880 681 367 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 2687 3006 4246 5147 5409 Size: 197 926 135 609 319 1240 901 262 Cuts at: 5409 820 Size: 1553 Fragments arranged by size: 1553 1240 926 901 609 319 262 197 135 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3500 1132 Size: 2368 3774 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 2972 1517 Size: 1455 4687 StyI C'CwwG_G Cuts at: 513 1065 2004 2317 2331 3581 513 Size: 552 939 313 14 1250 3074 Fragments arranged by size: 3074 1250 939 552 313 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4169 4186 4339 4524 4969 325 Size: 1255 2589 17 153 185 445 1498 Fragments arranged by size: 2589 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2268 2931 4388 Size: 274 80 33 51 1443 291 663 1457 Cuts at: 4388 6100 96 Size: 1712 138 Fragments arranged by size: 1712 1457 1443 663 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2298 2330 6102 6134 2298 Size: 32 3772 32 2306 Fragments arranged by size: 3772 2306 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2420 2420 Size: 6142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 2292 4271 2292 Size: 1979 4163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3950 276 Size: 53 83 186 936 2416 2468 Fragments arranged by size: 2468 2416 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI