(Circular) (Six-base) MAPSORT of: pFN21AF0040.seq Check: 8769 from: 1 to: 7855 pFN21AF0040 7855 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4304 4304 Size: 7855 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5665 278 Size: 53 83 186 936 4129 2468 Fragments arranged by size: 4129 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2150 4644 5198 1937 Size: 213 2494 554 4594 Fragments arranged by size: 4594 2494 554 213 Acc65I G'GTAC_C Cuts at: 4690 5176 4690 Size: 486 7369 AclI AA'CG_TT Cuts at: 1563 5982 6355 1563 Size: 4419 373 3063 Fragments arranged by size: 4419 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2735 3001 3815 4316 4667 5918 7302 7570 Size: 1103 266 814 501 351 1251 1384 268 Cuts at: 7570 1632 Size: 1917 Fragments arranged by size: 1917 1384 1251 1103 814 501 351 268 266 AfeI AGC'GCT Cuts at: 3914 4542 3914 Size: 628 7227 AflII C'TTAA_G Cuts at: 820 1017 3152 820 Size: 197 2135 5523 Fragments arranged by size: 5523 2135 197 AflIII A'CryG_T Cuts at: 1203 6754 7731 1203 Size: 5551 977 1327 Fragments arranged by size: 5551 1327 977 AgeI A'CCGG_T Cuts at: 6686 6686 Size: 7855 AhdI GACnn_n'nnGTC Cuts at: 3664 6584 3664 Size: 2920 4935 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7855 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7855 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3755 3787 4593 4625 3755 Size: 32 806 32 6985 Fragments arranged by size: 6985 806 32 32 AlwNI CAG_nnn'CTG Cuts at: 2572 4037 4618 5007 5598 7170 2572 Size: 1465 581 389 591 1572 3257 Fragments arranged by size: 3257 1572 1465 591 581 389 ApaI G_GGCC'C Cuts at: 5102 5102 Size: 7855 ApaLI G'TGCA_C Cuts at: 5912 7068 5912 Size: 1156 6699 ApoI r'AATT_y Cuts at: 1493 2661 2995 4130 4660 5164 5390 5542 7651 Size: 1168 334 1135 530 504 226 152 2109 Cuts at: 7651 1493 Size: 1697 Fragments arranged by size: 2109 1697 1168 1135 530 504 334 226 152 AseI AT'TA_AT Cuts at: 160 1032 6409 160 Size: 872 5377 1606 Fragments arranged by size: 5377 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7855 AvaI C'yCGr_G Cuts at: 1870 1943 5180 1870 Size: 73 3237 4545 Fragments arranged by size: 4545 3237 73 BamHI G'GATC_C Cuts at: 5185 5552 5185 Size: 367 7488 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2386 3461 4316 4690 5176 Size: 325 217 627 599 1075 855 374 486 Cuts at: 5176 6631 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1075 855 627 599 486 374 325 217 BanII G_rGCy'C Cuts at: 1391 5102 5174 1391 Size: 3711 72 4072 Fragments arranged by size: 4072 3711 72 BbeI G_GCGC'C Cuts at: 3465 3465 Size: 7855 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2028 2261 2449 4564 928 Size: 971 129 233 188 2115 4219 Fragments arranged by size: 4219 2115 971 233 188 129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6046 6080 1903 Size: 34 4109 34 3678 Fragments arranged by size: 4109 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3044 5747 6957 3044 Size: 2703 1210 3942 Fragments arranged by size: 3942 2703 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7855 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4233 4365 4930 Size: 107 122 71 631 605 2561 132 565 Cuts at: 4930 5017 6466 136 Size: 87 1449 1525 Fragments arranged by size: 2561 1525 1449 631 605 565 132 122 107 87 71 BglII A'GATC_T Cuts at: 2746 4045 7850 2746 Size: 1299 3805 2751 Fragments arranged by size: 3805 2751 1299 BlpI GC'TnA_GC Cuts at: 2820 5272 2820 Size: 2452 5403 Bme1580I G_kGCm'C Cuts at: 1790 2283 3396 4157 4168 5102 5916 7072 1790 Size: 493 1113 761 11 934 814 1156 2573 Fragments arranged by size: 2573 1156 1113 934 814 761 493 11 BmgBI CAC'GTC Cuts at: 1318 3661 4570 1318 Size: 2343 909 4603 Fragments arranged by size: 4603 2343 909 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3056 5586 6544 Size: 416 53 83 399 24 1639 2530 958 Cuts at: 6544 442 Size: 1753 Fragments arranged by size: 2530 1753 1639 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7855 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2598 4995 2598 Size: 2397 5458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2546 2936 3361 4112 6515 1239 Size: 1307 390 425 751 2403 2579 Fragments arranged by size: 2579 2403 1307 751 425 390 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3794 3961 5980 6845 7143 3794 Size: 167 2019 865 298 4506 Fragments arranged by size: 4506 2019 865 298 167 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2912 3983 6518 882 Size: 617 1413 1071 2535 2219 Fragments arranged by size: 2535 2219 1413 1071 617 BsaAI yAC'GTr Cuts at: 493 1204 2686 5646 493 Size: 711 1482 2960 2702 Fragments arranged by size: 2960 2702 1482 711 BsaBI GATnn'nnATC Cuts at: 4044 7849 4044 Size: 3805 4050 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3462 3741 3803 5662 Size: 53 83 186 936 1929 279 62 1859 Cuts at: 5662 6044 6679 275 Size: 382 635 1451 Fragments arranged by size: 1929 1859 1451 936 635 382 279 186 83 62 53 BsaWI w'CCGG_w Cuts at: 2776 4460 6288 6686 6960 7107 7518 2776 Size: 1684 1828 398 274 147 411 3113 Fragments arranged by size: 3113 1828 1684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2878 2908 2909 2939 3175 Size: 30 165 30 1475 30 1 30 236 Cuts at: 3175 3205 4223 4253 4442 4472 4490 4520 4593 Size: 30 1018 30 189 30 18 30 73 Cuts at: 4593 4623 4921 4951 1178 Size: 30 298 30 4082 Fragments arranged by size: 4082 1475 1018 298 236 189 165 73 30 30 30 30 30 30 30 30 30 30 18 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3886 3949 4001 4097 4228 1186 Size: 2700 63 52 96 131 4813 Fragments arranged by size: 4813 2700 131 96 63 52 BseYI C'CCAG_C Cuts at: 3726 4168 7058 7554 3726 Size: 442 2890 496 4027 Fragments arranged by size: 4027 2890 496 442 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2098 2897 3536 3596 3598 3691 3920 Size: 573 362 799 639 60 2 93 229 Cuts at: 3920 4137 4247 4529 1163 Size: 217 110 282 4489 Fragments arranged by size: 4489 799 639 573 362 282 229 217 110 93 60 2 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4270 5088 5305 6066 7094 1540 Size: 275 186 2269 818 217 761 1028 2301 Fragments arranged by size: 2301 2269 1028 818 761 275 217 186 BsiHKAI G_wGCw'C Cuts at: 5916 6001 7072 5916 Size: 85 1071 6699 Fragments arranged by size: 6699 1071 85 BsmI GAATG_Cn' Cuts at: 2504 2545 4408 5366 5459 2504 Size: 41 1863 958 93 4900 Fragments arranged by size: 4900 1863 958 93 41 BsmBI CGTCTCn'nnnn_ Cuts at: 3195 3340 3375 3794 3195 Size: 145 35 419 7256 Fragments arranged by size: 7256 419 145 35 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 3396 4157 4168 5102 5174 5916 Size: 399 493 1113 761 11 934 72 742 Cuts at: 5916 6001 7072 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1113 1071 934 761 742 493 399 85 72 11 BspEI T'CCGG_A Cuts at: 7518 7518 Size: 7855 BspHI T'CATG_A Cuts at: 2656 5744 7474 2656 Size: 3088 1730 3037 Fragments arranged by size: 3088 3037 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4304 4968 5210 844 Size: 3460 664 242 3489 Fragments arranged by size: 3489 3460 664 242 BsrBI CCG'CTC Cuts at: 1394 1823 3502 4345 5742 1394 Size: 429 1679 843 1397 3507 Fragments arranged by size: 3507 1679 1397 843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3216 3694 3714 3752 6350 6524 65 Size: 1183 1968 478 20 38 2598 174 1396 Fragments arranged by size: 2598 1968 1396 1183 478 174 38 20 BsrFI r'CCGG_y Cuts at: 1700 1810 3971 6499 6686 1700 Size: 110 2161 2528 187 2869 Fragments arranged by size: 2869 2528 2161 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7855 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7855 BssSI C'ACGA_G Cuts at: 2799 3626 4472 4547 5915 6927 2799 Size: 827 846 75 1368 1012 3727 Fragments arranged by size: 3727 1368 1012 846 827 75 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7855 BstBI TT'CG_AA Cuts at: 3069 4400 6659 3069 Size: 1331 2259 4265 Fragments arranged by size: 4265 2259 1331 BstEII G'GTnAC_C Cuts at: 1184 3227 1184 Size: 2043 5812 BstXI CCAn_nnnn'nTGG Cuts at: 2099 4838 2099 Size: 2739 5116 BstYI r'GATC_y Cuts at: 1972 2395 2746 2976 3780 4045 4976 5185 5552 Size: 423 351 230 804 265 931 209 367 Cuts at: 5552 5937 5954 7406 7492 7504 7850 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 931 804 423 385 367 351 346 265 230 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2098 2694 3465 3487 3918 4597 Size: 552 939 94 596 771 22 431 679 Cuts at: 4597 513 Size: 3771 Fragments arranged by size: 3771 939 771 679 596 552 431 94 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2455 3330 487 Size: 683 56 1229 875 5012 Fragments arranged by size: 5012 1229 875 683 56 BtsI GCAGTG_nn' Cuts at: 2643 4484 5371 6165 6185 2643 Size: 1841 887 794 20 4313 Fragments arranged by size: 4313 1841 887 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7855 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7820 DraI TTT'AAA Cuts at: 2680 5159 2680 Size: 2479 5376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4337 6862 809 Size: 507 3021 2525 1802 Fragments arranged by size: 3021 2525 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3622 4267 4465 Size: 54 1425 50 275 18 1792 645 198 Cuts at: 4465 5009 5302 6191 7820 8 Size: 544 293 889 1629 43 Fragments arranged by size: 1792 1629 1425 889 645 544 293 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4267 5302 1537 Size: 275 2455 1035 4090 Fragments arranged by size: 4090 2455 1035 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2184 2313 2370 4172 5785 1353 Size: 831 129 57 1802 1613 3423 Fragments arranged by size: 3423 1802 1613 831 129 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4274 4289 6442 6816 6962 387 Size: 1064 138 2685 15 2153 374 146 1280 Fragments arranged by size: 2685 2153 1280 1064 374 146 138 15 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2546 2735 2936 3001 3361 3815 4112 Size: 393 914 189 201 65 360 454 297 Cuts at: 4112 4316 4667 5918 6515 7302 7570 1239 Size: 204 351 1251 597 787 268 1524 Fragments arranged by size: 1524 1251 914 787 597 454 393 360 351 297 268 204 201 189 65 EcoNI CCTnn'n_nnAGG Cuts at: 3868 3868 Size: 7855 EcoO109I rG'GnC_Cy Cuts at: 3892 4087 4423 4503 5098 3892 Size: 195 336 80 595 6649 Fragments arranged by size: 6649 595 336 195 80 EcoRI G'AATT_C Cuts at: 4130 4660 5164 5542 4130 Size: 530 504 378 6443 Fragments arranged by size: 6443 530 504 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2239 4588 2239 Size: 2349 5506 FspI TGC'GCA Cuts at: 6361 6361 Size: 7855 HaeII r_GCGC'y Cuts at: 1678 3465 3916 4544 7002 1678 Size: 1787 451 628 2458 2531 Fragments arranged by size: 2531 2458 1787 628 451 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2503 2535 2927 2959 3175 Size: 32 135 32 931 32 392 32 216 Cuts at: 3175 3207 3650 3682 4223 4255 6496 6528 6570 Size: 32 443 32 541 32 2241 32 42 Cuts at: 6570 6602 1373 Size: 32 2626 Fragments arranged by size: 2626 2241 931 541 443 392 216 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5199 5445 669 Size: 988 281 3261 246 3079 Fragments arranged by size: 3261 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2852 748 Size: 2104 5751 HpaI GTT'AAC Cuts at: 5445 5445 Size: 7855 KasI G'GCGC_C Cuts at: 3461 3461 Size: 7855 KpnI G_GTAC'C Cuts at: 4694 5180 4694 Size: 486 7369 MfeI C'AATT_G Cuts at: 5454 5454 Size: 7855 MluI A'CGCG_T Cuts at: 7731 7731 Size: 7855 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4050 6969 7153 1305 Size: 2745 2919 184 2007 Fragments arranged by size: 2919 2745 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4467 5011 10 Size: 54 1425 343 2635 544 2854 Fragments arranged by size: 2854 2635 1425 544 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5813 6172 6331 518 Size: 648 4647 359 159 2042 Fragments arranged by size: 4647 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2824 3726 4246 4838 5271 5948 7096 7341 Size: 1218 902 520 592 433 677 1148 245 Cuts at: 7341 1606 Size: 2120 Fragments arranged by size: 2120 1218 1148 902 677 592 520 433 245 NaeI GCC'GGC Cuts at: 3973 3973 Size: 7855 NarI GG'CG_CC Cuts at: 3462 3462 Size: 7855 NcoI C'CATG_G Cuts at: 513 1065 2004 2098 2694 3465 3487 513 Size: 552 939 94 596 771 22 4881 Fragments arranged by size: 4881 939 771 596 552 94 22 NdeI CA'TA_TG Cuts at: 387 387 Size: 7855 NgoMIV G'CCGG_C Cuts at: 3971 3971 Size: 7855 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7855 NotI GC'GGCC_GC Cuts at: 5302 5302 Size: 7855 NspI r_CATG'y Cuts at: 1132 1229 4253 5213 6758 1132 Size: 97 3024 960 1545 2229 Fragments arranged by size: 3024 2229 1545 960 97 PasI CC'CwG_GG Cuts at: 1348 5095 1348 Size: 3747 4108 PciI A'CATG_T Cuts at: 6754 6754 Size: 7855 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 1108 Size: 1245 6610 PmeI GTTT'AAAC Cuts at: 5159 5159 Size: 7855 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2353 2385 2459 2491 4593 4625 5924 5956 7437 Size: 32 74 32 2102 32 1299 32 1481 Cuts at: 7437 7469 2353 Size: 32 2739 Fragments arranged by size: 2739 2102 1481 1299 74 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4087 4423 4087 Size: 336 7519 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7855 PsiI TTA'TAA Cuts at: 5425 5425 Size: 7855 PspOMI G'GGCC_C Cuts at: 5098 5098 Size: 7855 PstI C_TGCA'G Cuts at: 830 3762 5207 830 Size: 2932 1445 3478 Fragments arranged by size: 3478 2932 1445 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7855 PvuII CAG'CTG Cuts at: 2824 4838 2824 Size: 2014 5841 RsrII CG'GwC_CG Cuts at: 5151 5151 Size: 7855 SalI G'TCGA_C Cuts at: 1936 5197 1936 Size: 3261 4594 SapI GCTCTTCn'nnn_ Cuts at: 2184 2370 2184 Size: 186 7669 SbfI CC_TGCA'GG Cuts at: 5207 5207 Size: 7855 ScaI AGT'ACT Cuts at: 6103 7815 6103 Size: 1712 6143 SfcI C'TryA_G Cuts at: 826 1045 3758 4003 4493 4641 5203 6338 7019 Size: 219 2713 245 490 148 562 1135 681 Cuts at: 7019 7210 826 Size: 191 1471 Fragments arranged by size: 2713 1471 1135 681 562 490 245 219 191 148 SfiI GGCCn_nnn'nGGCC Cuts at: 5017 5017 Size: 7855 SfoI GGC'GCC Cuts at: 3463 3463 Size: 7855 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7855 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7855 SmaI CCC'GGG Cuts at: 1872 5182 1872 Size: 3310 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3152 3809 3976 5959 6860 7122 Size: 197 926 1209 657 167 1983 901 262 Cuts at: 7122 820 Size: 1553 Fragments arranged by size: 1983 1553 1209 926 901 657 262 197 167 SnaBI TAC'GTA Cuts at: 493 5646 493 Size: 5153 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7855 SphI G_CATG'C Cuts at: 1132 5213 1132 Size: 4081 3774 SspI AAT'ATT Cuts at: 5 52 5779 5 Size: 47 5727 2081 Fragments arranged by size: 5727 2081 47 StuI AGG'CCT Cuts at: 1517 5020 1517 Size: 3503 4352 StyI C'CwwG_G Cuts at: 513 1065 2004 2098 2694 3456 3465 3487 3736 Size: 552 939 94 596 762 9 22 249 Cuts at: 3736 5294 513 Size: 1558 3074 Fragments arranged by size: 3074 1558 939 762 596 552 249 94 22 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5882 5899 6052 6237 6682 325 Size: 1255 4302 17 153 185 445 1498 Fragments arranged by size: 4302 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6101 7813 96 Size: 274 80 33 51 1443 4124 1712 138 Fragments arranged by size: 4124 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2909 2941 4490 4522 4921 4953 7815 7847 2909 Size: 32 1549 32 399 32 2862 32 2917 Fragments arranged by size: 2917 2862 1549 399 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3187 4109 1316 Size: 1871 922 5062 Fragments arranged by size: 5062 1871 922 XbaI T'CTAG_A Cuts at: 4048 5191 4048 Size: 1143 6712 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2812 3463 2812 Size: 651 7204 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7855 XmaI C'CCGG_G Cuts at: 1870 5180 1870 Size: 3310 4545 XmnI GAAnn'nnTTC Cuts at: 3087 4680 4858 5984 3087 Size: 1593 178 1126 4958 Fragments arranged by size: 4958 1593 1126 178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5663 276 Size: 53 83 186 936 4129 2468 Fragments arranged by size: 4129 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI SacI SacII SanDI SexAI SrfI SwaI