(Circular) (Six-base) MAPSORT of: pFN21AB5209.seq Check: 7300 from: 1 to: 6286 pFN21AB5209 6286 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3629 1937 Size: 1692 4594 Acc65I G'GTAC_C Cuts at: 3607 3607 Size: 6286 AclI AA'CG_TT Cuts at: 1563 3585 4413 4786 1563 Size: 2022 828 373 3063 Fragments arranged by size: 3063 2022 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2255 4349 5733 6001 1632 Size: 623 2094 1384 268 1917 Fragments arranged by size: 2094 1917 1384 623 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 2307 2677 3099 5185 6162 1203 Size: 1104 370 422 2086 977 1327 Fragments arranged by size: 2086 1327 1104 977 422 370 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 5015 5015 Size: 6286 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6286 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2116 3182 3206 3356 3562 1184 Size: 932 1066 24 150 206 3908 Fragments arranged by size: 3908 1066 932 206 150 24 AlwNI CAG_nnn'CTG Cuts at: 2857 4029 5601 2857 Size: 1172 1572 3542 Fragments arranged by size: 3542 1572 1172 ApaI G_GGCC'C Cuts at: 2708 3509 2708 Size: 801 5485 ApaLI G'TGCA_C Cuts at: 4343 5499 4343 Size: 1156 5130 ApoI r'AATT_y Cuts at: 1493 2969 3595 3821 3973 6082 1493 Size: 1476 626 226 152 2109 1697 Fragments arranged by size: 2109 1697 1476 626 226 152 AseI AT'TA_AT Cuts at: 160 1032 4840 160 Size: 872 3808 1606 Fragments arranged by size: 3808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 3611 1870 Size: 73 1668 4545 Fragments arranged by size: 4545 1668 73 BamHI G'GATC_C Cuts at: 3424 3616 3983 3424 Size: 192 367 5727 Fragments arranged by size: 5727 367 192 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2100 3519 3607 5062 618 Size: 325 217 627 313 1419 88 1455 1842 Fragments arranged by size: 1842 1455 1419 627 325 313 217 88 BanII G_rGCy'C Cuts at: 1391 2090 2708 2916 3393 3509 3605 1391 Size: 699 618 208 477 116 96 4072 Fragments arranged by size: 4072 699 618 477 208 116 96 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2120 2285 928 Size: 971 221 165 4929 Fragments arranged by size: 4929 971 221 165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3538 3572 4477 4511 1903 Size: 34 1601 34 905 34 3678 Fragments arranged by size: 3678 1601 905 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 3367 1548 Size: 1819 4467 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 2873 4897 Size: 107 122 71 631 605 365 836 2024 Cuts at: 4897 136 Size: 1525 Fragments arranged by size: 2024 1525 836 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 2802 6281 2802 Size: 3479 2807 BlpI GC'TnA_GC Cuts at: 3703 3703 Size: 6286 Bme1580I G_kGCm'C Cuts at: 1790 2708 3509 4347 5503 1790 Size: 918 801 838 1156 2573 Fragments arranged by size: 2573 1156 918 838 801 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3538 4017 4975 Size: 416 53 83 399 24 2121 479 958 Cuts at: 4975 442 Size: 1753 Fragments arranged by size: 2121 1753 958 479 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2411 2486 3152 3302 4946 1239 Size: 1172 75 666 150 1644 2579 Fragments arranged by size: 2579 1644 1172 666 150 75 Bpu10I CC'TnA_GC Cuts at: 3147 3147 Size: 6286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3341 4411 5276 5574 3341 Size: 1070 865 298 4053 Fragments arranged by size: 4053 1070 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4949 882 Size: 617 3450 2219 Fragments arranged by size: 3450 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3102 3211 4077 493 Size: 711 1898 109 866 2702 Fragments arranged by size: 2702 1898 866 711 109 BsaBI GATnn'nnATC Cuts at: 3404 6280 3404 Size: 2876 3410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2120 4093 4475 5110 Size: 53 83 186 936 587 1973 382 635 Cuts at: 5110 275 Size: 1451 Fragments arranged by size: 1973 1451 936 635 587 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3095 3125 1178 Size: 30 165 30 1692 30 4339 Fragments arranged by size: 4339 1692 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2270 2525 2528 2702 3149 1186 Size: 1081 3 255 3 174 447 4323 Fragments arranged by size: 4323 1081 447 255 174 3 3 BseYI C'CCAG_C Cuts at: 2571 2935 5489 5985 2571 Size: 364 2554 496 2872 Fragments arranged by size: 2872 2554 496 364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3152 3178 3180 1163 Size: 573 1416 26 2 4269 Fragments arranged by size: 4269 1416 573 26 2 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2402 3053 4347 4432 5503 2402 Size: 651 1294 85 1071 3185 Fragments arranged by size: 3185 1294 1071 651 85 BsiWI C'GTAC_G Cuts at: 3102 3102 Size: 6286 BsmI GAATG_Cn' Cuts at: 2081 2607 3797 3890 2081 Size: 526 1190 93 4477 Fragments arranged by size: 4477 1190 526 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2402 2708 2916 3053 3393 3509 Size: 399 300 312 306 208 137 340 116 Cuts at: 3509 3605 4347 4432 5503 1391 Size: 96 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 340 312 306 300 208 137 116 96 85 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 4175 5905 4175 Size: 1730 4556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3641 844 Size: 2797 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4173 1394 Size: 429 2350 3507 Fragments arranged by size: 3507 2350 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3556 3562 4781 4955 65 Size: 1183 2308 6 1219 174 1396 Fragments arranged by size: 2308 1396 1219 1183 174 6 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 4930 5117 1700 Size: 110 241 2879 187 2869 Fragments arranged by size: 2879 2869 241 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6286 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 2927 4346 5358 2927 Size: 1419 1012 3855 Fragments arranged by size: 3855 1419 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2149 794 Size: 1355 4931 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 2986 1184 Size: 1802 4484 BstXI CCAn_nnnn'nTGG Cuts at: 2035 3130 3516 2035 Size: 1095 386 4805 Fragments arranged by size: 4805 1095 386 BstYI r'GATC_y Cuts at: 1972 2259 2478 2802 3306 3405 3424 3616 3983 Size: 287 219 324 504 99 19 192 367 Cuts at: 3983 4368 4385 5837 5923 5935 6281 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 504 385 367 346 324 287 219 192 99 86 19 17 12 Bsu36I CC'TnA_GG Cuts at: 2547 2547 Size: 6286 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5734 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5547 Fragments arranged by size: 5547 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2142 2143 3368 3802 4596 4616 2100 Size: 42 1 1225 434 794 20 3770 Fragments arranged by size: 3770 1225 794 434 42 20 1 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2520 2715 Size: 54 1425 50 275 18 195 495 195 Cuts at: 2715 3733 4622 6251 8 Size: 1018 889 1629 43 Fragments arranged by size: 1629 1425 1018 889 495 275 195 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 3072 4216 1353 Size: 668 1051 1144 3423 Fragments arranged by size: 3423 1144 1051 668 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2411 2486 3152 3302 4349 4946 Size: 393 623 156 75 666 150 1047 597 Cuts at: 4946 5733 6001 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1047 787 666 623 597 393 268 156 150 75 EcoNI CCTnn'n_nnAGG Cuts at: 2978 3131 3413 2978 Size: 153 282 5851 Fragments arranged by size: 5851 282 153 EcoO109I rG'GnC_Cy Cuts at: 2705 2932 3505 2705 Size: 227 573 5486 Fragments arranged by size: 5486 573 227 EcoRI G'AATT_C Cuts at: 2969 3595 3973 2969 Size: 626 378 5282 Fragments arranged by size: 5282 626 378 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 5433 1678 Size: 3755 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4927 4959 5001 5033 1373 Size: 32 135 32 3355 32 42 32 2626 Fragments arranged by size: 3355 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3630 3876 669 Size: 988 281 1692 246 3079 Fragments arranged by size: 3079 1692 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6286 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 3611 3611 Size: 6286 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2148 3054 5400 5584 1305 Size: 843 906 2346 184 2007 Fragments arranged by size: 2346 2007 906 843 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2522 10 Size: 54 1425 343 195 495 3774 Fragments arranged by size: 3774 1425 495 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2612 4244 4603 4762 518 Size: 648 1446 1632 359 159 2042 Fragments arranged by size: 2042 1632 1446 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2222 2464 3702 4379 5527 5772 1606 Size: 616 242 1238 677 1148 245 2120 Fragments arranged by size: 2120 1238 1148 677 616 245 242 NaeI GCC'GGC Cuts at: 2053 2053 Size: 6286 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5734 NdeI CA'TA_TG Cuts at: 387 2198 2723 387 Size: 1811 525 3950 Fragments arranged by size: 3950 1811 525 NgoMIV G'CCGG_C Cuts at: 2051 2051 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2311 2681 2782 3166 3644 5189 1132 Size: 97 1082 370 101 384 478 1545 2229 Fragments arranged by size: 2229 1545 1082 478 384 370 101 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 2307 2677 5185 2307 Size: 370 2508 3408 Fragments arranged by size: 3408 2508 370 PflMI CCAn_nnn'nTGG Cuts at: 1108 2668 3091 1108 Size: 1560 423 4303 Fragments arranged by size: 4303 1560 423 PfoI T'CCnGG_A Cuts at: 2355 2355 Size: 6286 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PmlI CAC'GTG Cuts at: 3211 3211 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4355 4387 5868 5900 4355 Size: 32 1481 32 4741 Fragments arranged by size: 4741 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2932 2932 Size: 6286 PshAI GACnn'nnGTC Cuts at: 1613 2858 1613 Size: 1245 5041 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PspOMI G'GGCC_C Cuts at: 2704 3505 2704 Size: 801 5485 PstI C_TGCA'G Cuts at: 830 2154 3335 3638 830 Size: 1324 1181 303 3478 Fragments arranged by size: 3478 1324 1181 303 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 2222 2464 2222 Size: 242 6044 SalI G'TCGA_C Cuts at: 1936 3628 1936 Size: 1692 4594 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 4534 6246 4534 Size: 1712 4574 SfcI C'TryA_G Cuts at: 826 1045 2150 3331 3634 4769 5450 5641 826 Size: 219 1105 1181 303 1135 681 191 1471 Fragments arranged by size: 1471 1181 1135 1105 681 303 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3320 4390 5291 5553 820 Size: 197 926 1377 1070 901 262 1553 Fragments arranged by size: 1553 1377 1070 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 3166 3644 1132 Size: 2034 478 3774 Fragments arranged by size: 3774 2034 478 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 2666 1517 Size: 1149 5137 StyI C'CwwG_G Cuts at: 513 1065 2062 2667 2796 3725 513 Size: 552 997 605 129 929 3074 Fragments arranged by size: 3074 997 929 605 552 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4313 4330 4483 4668 5113 325 Size: 1255 2733 17 153 185 445 1498 Fragments arranged by size: 2733 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4532 6244 96 Size: 274 80 33 51 1443 2555 1712 138 Fragments arranged by size: 2555 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6246 6278 6246 Size: 32 6254 Tth111I GACn'n_nGTC Cuts at: 1316 2119 2210 1316 Size: 803 91 5392 Fragments arranged by size: 5392 803 91 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2854 2854 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 4415 4415 Size: 6286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI