(Circular) (Six-base) MAPSORT of: pFN21AB4277.seq Check: 4237 from: 1 to: 5980 pFN21AB4277 5980 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3323 1937 Size: 1386 4594 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2577 2847 3267 4043 5427 5695 1632 Size: 945 270 420 776 1384 268 1917 Fragments arranged by size: 1917 1384 945 776 420 270 268 AflII C'TTAA_G Cuts at: 820 1017 2927 820 Size: 197 1910 3873 Fragments arranged by size: 3873 1910 197 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 3032 4709 3032 Size: 1677 4303 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5980 AlwNI CAG_nnn'CTG Cuts at: 2744 3723 5295 2744 Size: 979 1572 3429 Fragments arranged by size: 3429 1572 979 ApaI G_GGCC'C Cuts at: 2395 2395 Size: 5980 ApaLI G'TGCA_C Cuts at: 4037 5193 4037 Size: 1156 4824 ApoI r'AATT_y Cuts at: 1493 2315 3289 3515 3667 5776 1493 Size: 822 974 226 152 2109 1697 Fragments arranged by size: 2109 1697 974 822 226 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 3305 1870 Size: 73 1362 4545 Fragments arranged by size: 4545 1362 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2401 2434 2401 Size: 33 5947 BamHI G'GATC_C Cuts at: 3259 3310 3677 3259 Size: 51 367 5562 Fragments arranged by size: 5562 367 51 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3301 4756 618 Size: 325 217 627 1514 1455 1842 Fragments arranged by size: 1842 1514 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2395 3299 1391 Size: 1004 904 4072 Fragments arranged by size: 4072 1004 904 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2669 3090 3195 3246 928 Size: 971 770 421 105 51 3662 Fragments arranged by size: 3662 971 770 421 105 51 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2764 3872 5082 2764 Size: 1108 1210 3662 Fragments arranged by size: 3662 1210 1108 BclI T'GATC_A Cuts at: 1548 2052 2749 1548 Size: 504 697 4779 Fragments arranged by size: 4779 697 504 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4591 136 Size: 107 122 71 631 605 2919 1525 Fragments arranged by size: 2919 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5975 5975 Size: 5980 BlpI GC'TnA_GC Cuts at: 3397 3397 Size: 5980 Bme1580I G_kGCm'C Cuts at: 1790 2395 2580 4041 5197 1790 Size: 605 185 1461 1156 2573 Fragments arranged by size: 2573 1461 1156 605 185 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2140 3711 4669 Size: 416 53 83 399 24 723 1571 958 Cuts at: 4669 442 Size: 1753 Fragments arranged by size: 1753 1571 958 723 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2517 4640 1239 Size: 905 373 2123 2579 Fragments arranged by size: 2579 2123 905 373 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4105 4970 5268 4105 Size: 865 298 4817 Fragments arranged by size: 4817 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4643 882 Size: 617 3144 2219 Fragments arranged by size: 3144 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 5974 5974 Size: 5980 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3787 4169 4804 275 Size: 53 83 186 936 2254 382 635 1451 Fragments arranged by size: 2254 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2419 2449 1178 Size: 30 165 30 1016 30 4709 Fragments arranged by size: 4709 1016 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5980 BseYI C'CCAG_C Cuts at: 2132 5183 5679 2132 Size: 3051 496 2433 Fragments arranged by size: 3051 2433 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 1163 Size: 573 540 4867 Fragments arranged by size: 4867 573 540 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4041 4126 5197 4041 Size: 85 1071 4824 Fragments arranged by size: 4824 1071 85 BsmI GAATG_Cn' Cuts at: 2190 2935 3491 3584 2190 Size: 745 556 93 4586 Fragments arranged by size: 4586 745 556 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2525 2525 Size: 5980 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2395 2580 3299 4041 4126 5197 1391 Size: 399 605 185 719 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 719 605 399 185 85 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3335 844 Size: 2491 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3867 1394 Size: 429 2044 3507 Fragments arranged by size: 3507 2044 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4624 4811 1700 Size: 110 2814 187 2869 Fragments arranged by size: 2869 2814 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5980 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 2979 1184 Size: 1795 4185 BstYI r'GATC_y Cuts at: 1972 2488 2908 3259 3310 3677 4062 4079 5531 Size: 516 420 351 51 367 385 17 1452 Cuts at: 5531 5617 5629 5975 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 516 420 385 367 351 346 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2524 513 Size: 552 939 520 3969 Fragments arranged by size: 3969 939 552 520 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2407 487 Size: 683 56 1181 4060 Fragments arranged by size: 4060 1181 683 56 BtsI GCAGTG_nn' Cuts at: 2043 2820 3496 4290 4310 2043 Size: 777 676 794 20 3713 Fragments arranged by size: 3713 794 777 676 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3081 3116 529 Size: 35 2517 35 3393 Fragments arranged by size: 3393 2517 35 35 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4987 809 Size: 507 3671 1802 Fragments arranged by size: 3671 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3427 4316 Size: 54 1425 50 275 18 177 1420 889 Cuts at: 4316 5945 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1420 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3910 1353 Size: 2557 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4567 4941 5087 387 Size: 1064 138 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2517 2577 2847 3267 4043 4640 Size: 393 512 373 60 270 420 776 597 Cuts at: 4640 5427 5695 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 776 597 512 420 393 373 270 268 60 EcoO109I rG'GnC_Cy Cuts at: 2391 2391 Size: 5980 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 EcoRV GAT'ATC Cuts at: 3135 3135 Size: 5980 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2686 2686 Size: 5980 FspI TGC'GCA Cuts at: 4486 4486 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 5127 1678 Size: 3449 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2104 2136 3018 3050 3082 Size: 32 135 32 532 32 882 32 32 Cuts at: 3082 4621 4653 4695 4727 1373 Size: 1539 32 42 32 2626 Fragments arranged by size: 2626 1539 882 532 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3324 3570 669 Size: 988 281 1386 246 3079 Fragments arranged by size: 3079 1386 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5094 5278 1305 Size: 3789 184 2007 Fragments arranged by size: 3789 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4158 Fragments arranged by size: 4158 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2955 3203 3938 4297 4456 518 Size: 648 1789 248 735 359 159 2042 Fragments arranged by size: 2042 1789 735 648 359 248 159 MspA1I CmG'CkG Cuts at: 1606 2885 3396 4073 5221 5466 1606 Size: 1279 511 677 1148 245 2120 Fragments arranged by size: 2120 1279 1148 677 511 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 3338 4883 1132 Size: 97 2109 1545 2229 Fragments arranged by size: 2229 2109 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5980 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 2122 1108 Size: 1014 4966 PfoI T'CCnGG_A Cuts at: 3146 3146 Size: 5980 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2517 3274 1613 Size: 904 757 4319 Fragments arranged by size: 4319 904 757 PsiI TTA'TAA Cuts at: 3231 3550 3231 Size: 319 5661 PspOMI G'GGCC_C Cuts at: 2391 2391 Size: 5980 PstI C_TGCA'G Cuts at: 830 3332 830 Size: 2502 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 PvuII CAG'CTG Cuts at: 2885 2885 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 3328 4463 5144 5335 826 Size: 219 2283 1135 681 191 1471 Fragments arranged by size: 2283 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 3307 1872 Size: 1435 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2927 4084 4985 5247 820 Size: 197 926 984 1157 901 262 1553 Fragments arranged by size: 1553 1157 984 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 2964 3904 5 Size: 47 2912 940 2081 Fragments arranged by size: 2912 2081 940 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5980 StyI C'CwwG_G Cuts at: 513 1065 2004 3164 3419 513 Size: 552 939 1160 255 3074 Fragments arranged by size: 3074 1160 939 552 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3157 4007 4024 4177 4362 4807 325 Size: 1255 1577 850 17 153 185 445 1498 Fragments arranged by size: 1577 1498 1255 850 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4226 5938 96 Size: 274 80 33 51 1443 2249 1712 138 Fragments arranged by size: 2249 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5940 5972 5940 Size: 32 5948 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5980 XmaI C'CCGG_G Cuts at: 1870 3305 1870 Size: 1435 4545 XmnI GAAnn'nnTTC Cuts at: 2183 4109 2183 Size: 1926 4054 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI