Restriction map for
pFN21AB4033
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccII AccIII AflIII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvrII BanII BbeI BcgI
BcnI BlnI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI FseI
HindIII HpaI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PmeI
PshAI PshBI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AclI 1996 AACGTT AA^CGTT
AflII 1945 CTTAAG C^TTAAG
AgeI 2078 ACCGGT A^CCGGT
AocI 1278 CCTAAGG CC^T[ACGT]AGG
Asp700I 829 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2960 CTCGGG C^[CT]CG[GA]G
AviII 2874 TGCGCA TGC^GCA
BbrPI 1355 CACGTG CAC^GTG
BbvCI 988 GCTGAGG CCTCAGC(-5/-2)
BfrI 1945 CTTAAG C^TTAAG
BglI 2722 GCCTTTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1355 CACGTG [CT]AC^GT[GA]
BsaWI 2078 ACCGGT [AT]^CCGG[AT]
BsgI 2459 GTGCAG GTGCAG(16/14)
BsoBI 2960 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2843 TGTACA T^GTACA
BsrDI 1668 CATTGC GCAATG(2/0)
BsrFI 2078 ACCGGT [GA]^CCGG[CT]
BsrGI 2843 TGTACA T^GTACA
BstAPI 2993 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2627 TTCGAA TT^CGAA
Bsu36I 1278 CCTAAGG CC^T[ACGT]AGG
Cfr10I 2078 ACCGGT [GA]^CCGG[CT]
DrdI 994 GACTATCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1278 CCTAAGG CC^T[ACGT]AGG
EcoRV 2584 GATATC GAT^ATC
EcoT22I 1437 ATGCAT ATGCA^T
FspI 2874 TGCGCA TGC^GCA
HaeII 907 GGCGCT [GA]GCGC^[CT]
HapII 2079 CCGG C^CGG
HgaI 2709 GCGTC GACGC(5/10)
HpaII 2079 CCGG C^CGG
MspA1I 1239 CAGCTG C[AC]G^C[GT]G
MspI 2079 CCGG C^CGG
NdeI 3242 CATATG CA^TATG
NsiI 1437 ATGCAT ATGCA^T
NspV 2627 TTCGAA TT^CGAA
PinAI 2078 ACCGGT A^CCGGT
PmaCI 1355 CACGTG CAC^GTG
PmlI 1355 CACGTG CAC^GTG
PpuMI 1304 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1996 AACGTT AA^CGTT
PvuII 1239 CAGCTG CAG^CTG
ScaI 3499 AGTACT AGT^ACT
SfuI 2627 TTCGAA TT^CGAA
SmlI 1945 CTTAAG C^T[CT][GA]AG
SphI 1595 GCATGC GCATG^C
SspBI 2843 TGTACA T^GTACA
StuI 507 AGGCCT AGG^CCT
XmnI 829 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 1939 GACGTC GACGT^C
2739 GACGTC
AccI 396 GTCTAC GT^[AC][GT]AC
2643 GTCTAC
AcyI 1939 GACGTC G[GA]^CG[CT]C
2739 GACGTC
AlwNI 1380 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
3529 CAGCTCCTG
BamHI 1543 GGATCC G^GATCC
2084 GGATCC
BciVI 453 GTATCC GTATCC(6/5)
2532 GTATCC
BglII 1481 AGATCT A^GATCT
1488 AGATCT
BsaHI 1939 GACGTC G[GA]^CG[CT]C
2739 GACGTC
BsaI 2507 GAGACC GGTCTC(1/5)
3691 GAGACC
CfoI 908 GCGC GCG^C
2875 GCGC
DraI 1918 TTTAAA TTT^AAA
3333 TTTAAA
DraII 868 AGGCCCC [GA]G^G[ACGT]CC[CT]
1304 GGGTCCC
DraIII 1529 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3452 CACTTTGTG
EcoO109I 868 AGGCCCC [GA]G^G[ACGT]CC[CT]
1304 GGGTCCC
FauI 181 GCGGG CCCGC(4/6)
1935 GCGGG
HhaI 908 GCGC GCG^C
2875 GCGC
Hin1I 1939 GACGTC G[GA]^CG[CT]C
2739 GACGTC
HinP1I 908 GCGC G^CGC
2875 GCGC
MfeI 365 CAATTG C^AATTG
2193 CAATTG
MunI 365 CAATTG C^AATTG
2193 CAATTG
NcoI 261 CCATGG C^CATGG
1963 CCATGG
NspI 550 GCATGT [GA]CATG^[CT]
1595 GCATGC
PstI 1658 CTGCAG CTGCA^G
3464 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BanI BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII