(Circular) (Six-base) MAPSORT of: pFN21AB4033.seq Check: 7452 from: 1 to: 8491 pFN21AB4033 8491 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3948 4748 6301 278 Size: 53 83 186 936 2412 800 1553 2468 Fragments arranged by size: 2468 2412 1553 936 800 186 83 53 AccI GT'mk_AC Cuts at: 1937 2402 4649 5834 1937 Size: 465 2247 1185 4594 Fragments arranged by size: 4594 2247 1185 465 Acc65I G'GTAC_C Cuts at: 5812 5812 Size: 8491 AclI AA'CG_TT Cuts at: 1563 4002 6618 6991 1563 Size: 2439 2616 373 3063 Fragments arranged by size: 3063 2616 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2064 2601 2915 3051 3588 4739 5076 5366 Size: 432 537 314 136 537 1151 337 290 Cuts at: 5366 6554 7938 8206 1632 Size: 1188 1384 268 1917 Fragments arranged by size: 1917 1384 1188 1151 537 537 432 337 314 290 268 136 AflII C'TTAA_G Cuts at: 820 1017 3950 820 Size: 197 2933 5361 Fragments arranged by size: 5361 2933 197 AflIII A'CryG_T Cuts at: 1203 7390 8367 1203 Size: 6187 977 1327 Fragments arranged by size: 6187 1327 977 AgeI A'CCGG_T Cuts at: 4083 7322 4083 Size: 3239 5252 AhdI GACnn_n'nnGTC Cuts at: 7220 7220 Size: 8491 AleI CACnn'nnGTG Cuts at: 1166 2226 5557 1166 Size: 1060 3331 4100 Fragments arranged by size: 4100 3331 1060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5603 1184 Size: 4419 4072 AlwNI CAG_nnn'CTG Cuts at: 3390 5539 6234 7806 3390 Size: 2149 695 1572 4075 Fragments arranged by size: 4075 2149 1572 695 ApaLI G'TGCA_C Cuts at: 6548 7704 6548 Size: 1156 7335 ApoI r'AATT_y Cuts at: 1493 2032 2075 2123 4034 5800 6026 6178 8287 Size: 539 43 48 1911 1766 226 152 2109 Cuts at: 8287 1493 Size: 1697 Fragments arranged by size: 2109 1911 1766 1697 539 226 152 48 43 AseI AT'TA_AT Cuts at: 160 1032 7045 160 Size: 872 6013 1606 Fragments arranged by size: 6013 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8491 AvaI C'yCGr_G Cuts at: 1870 1943 4965 5816 1870 Size: 73 3022 851 4545 Fragments arranged by size: 4545 3022 851 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3115 3148 3115 Size: 33 8458 BamHI G'GATC_C Cuts at: 3548 4089 5821 6188 3548 Size: 541 1732 367 5851 Fragments arranged by size: 5851 1732 541 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2846 3395 4162 5812 7267 Size: 325 217 627 1059 549 767 1650 1455 Cuts at: 7267 618 Size: 1842 Fragments arranged by size: 1842 1650 1455 1059 767 627 549 325 217 BanII G_rGCy'C Cuts at: 1391 5810 1391 Size: 4419 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2482 4796 4839 5389 5624 5727 5741 Size: 971 583 2314 43 550 235 103 14 Cuts at: 5741 5756 928 Size: 15 3663 Fragments arranged by size: 3663 2314 971 583 550 235 103 43 15 14 BbvCI CC'TCA_GC Cuts at: 2994 2994 Size: 8491 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6682 6716 1903 Size: 34 50 34 4661 34 3678 Fragments arranged by size: 4661 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2469 4548 6383 7593 2469 Size: 2079 1835 1210 3367 Fragments arranged by size: 3367 2079 1835 1210 BclI T'GATC_A Cuts at: 1548 4506 4858 5490 1548 Size: 2958 352 632 4549 Fragments arranged by size: 4549 2958 632 352 BfrBI ATG'CAT Cuts at: 3444 3444 Size: 8491 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4733 7102 136 Size: 107 122 71 631 605 3061 2369 1525 Fragments arranged by size: 3061 2369 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3486 3493 8486 3486 Size: 7 4993 3491 Fragments arranged by size: 4993 3491 7 BlpI GC'TnA_GC Cuts at: 5908 5908 Size: 8491 Bme1580I G_kGCm'C Cuts at: 1790 3243 3398 5582 6552 7708 1790 Size: 1453 155 2184 970 1156 2573 Fragments arranged by size: 2573 2184 1453 1156 970 155 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8491 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2312 3316 3400 Size: 416 53 83 399 24 895 1004 84 Cuts at: 3400 4803 6222 7180 442 Size: 1403 1419 958 1753 Fragments arranged by size: 1753 1419 1403 1004 958 895 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8491 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5315 5315 Size: 8491 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3226 3998 4796 7151 1239 Size: 1987 772 798 2355 2579 Fragments arranged by size: 2579 2355 1987 798 772 Bpu10I CC'TnA_GC Cuts at: 2994 3612 4679 4905 2994 Size: 618 1067 226 6580 Fragments arranged by size: 6580 1067 618 226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6616 7481 7779 6616 Size: 865 298 7328 Fragments arranged by size: 7328 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4506 5690 7154 882 Size: 617 3007 1184 1464 2219 Fragments arranged by size: 3007 2219 1464 1184 617 BsaAI yAC'GTr Cuts at: 493 1204 3362 6282 493 Size: 711 2158 2920 2702 Fragments arranged by size: 2920 2702 2158 711 BsaBI GATnn'nnATC Cuts at: 8485 8485 Size: 8491 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3945 4745 6298 6680 Size: 53 83 186 936 2412 800 1553 382 Cuts at: 6680 7315 275 Size: 635 1451 Fragments arranged by size: 2412 1553 1451 936 800 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4083 6924 7322 7596 7743 8154 4083 Size: 2841 398 274 147 411 4420 Fragments arranged by size: 4420 2841 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2665 2695 1178 Size: 30 165 30 1262 30 6974 Fragments arranged by size: 6974 1262 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3210 3446 4784 1186 Size: 2024 236 1338 4893 Fragments arranged by size: 4893 2024 1338 236 BseYI C'CCAG_C Cuts at: 3242 7694 8190 3242 Size: 4452 496 3543 Fragments arranged by size: 4452 3543 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4485 1163 Size: 573 2749 5169 Fragments arranged by size: 5169 2749 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5941 6702 7730 1540 Size: 275 186 3940 761 1028 2301 Fragments arranged by size: 3940 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6552 6637 7708 6552 Size: 85 1071 7335 Fragments arranged by size: 7335 1071 85 BsmI GAATG_Cn' Cuts at: 2099 4404 4671 5548 6002 6095 2099 Size: 2305 267 877 454 93 4495 Fragments arranged by size: 4495 2305 877 454 267 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3243 3398 5582 5810 6552 6637 7708 Size: 399 1453 155 2184 228 742 85 1071 Cuts at: 7708 1391 Size: 2174 Fragments arranged by size: 2184 2174 1453 1071 742 399 228 155 85 BspEI T'CCGG_A Cuts at: 8154 8154 Size: 8491 BspHI T'CATG_A Cuts at: 6380 8110 6380 Size: 1730 6761 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5846 844 Size: 5002 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6378 1394 Size: 429 4555 3507 Fragments arranged by size: 4555 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3672 6986 7160 65 Size: 1183 2424 3314 174 1396 Fragments arranged by size: 3314 2424 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4083 7135 7322 1700 Size: 110 2273 3052 187 2869 Fragments arranged by size: 3052 2869 2273 187 110 BsrGI T'GTAC_A Cuts at: 96 4848 96 Size: 4752 3739 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8491 BssSI C'ACGA_G Cuts at: 6551 7563 6551 Size: 1012 7479 BstAPI GCAn_nnn'nTGC Cuts at: 794 5004 794 Size: 4210 4281 BstBI TT'CG_AA Cuts at: 4633 7295 4633 Size: 2662 5829 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8491 BstXI CCAn_nnnn'nTGG Cuts at: 3857 4793 4807 3857 Size: 936 14 7541 Fragments arranged by size: 7541 936 14 BstYI r'GATC_y Cuts at: 1972 2611 2826 3486 3493 3548 3832 4089 4570 Size: 639 215 660 7 55 284 257 481 Cuts at: 4570 5189 5219 5821 6188 6573 6590 8042 8128 Size: 619 30 602 367 385 17 1452 86 Cuts at: 8128 8140 8486 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 660 639 619 602 481 385 367 346 284 257 215 86 55 30 17 12 7 Bsu36I CC'TnA_GG Cuts at: 3284 3284 Size: 8491 BtgI C'CryG_G Cuts at: 513 1065 2266 3729 3968 513 Size: 552 1201 1463 239 5036 Fragments arranged by size: 5036 1463 1201 552 239 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3435 487 Size: 683 56 2209 5543 Fragments arranged by size: 5543 2209 683 56 BtsI GCAGTG_nn' Cuts at: 3243 4743 4771 5284 6007 6801 6821 3243 Size: 1500 28 513 723 794 20 4913 Fragments arranged by size: 4913 1500 794 723 513 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8491 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8456 DraI TTT'AAA Cuts at: 3925 5340 5795 3925 Size: 1415 455 6621 Fragments arranged by size: 6621 1415 455 DraIII CAC_nnn'GTG Cuts at: 3539 5462 3539 Size: 1923 6568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3005 7498 809 Size: 507 1689 4493 1802 Fragments arranged by size: 4493 1802 1689 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2328 2419 3726 Size: 54 1425 50 275 18 498 91 1307 Cuts at: 3726 3796 5938 6827 8456 8 Size: 70 2142 889 1629 43 Fragments arranged by size: 2142 1629 1425 1307 889 498 275 91 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5938 1537 Size: 275 4126 4090 Fragments arranged by size: 4126 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 3924 4495 5342 5514 6421 1353 Size: 690 1881 571 847 172 907 3423 Fragments arranged by size: 3423 1881 907 847 690 571 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3473 4829 7078 7452 7598 387 Size: 1064 138 1884 1356 2249 374 146 1280 Fragments arranged by size: 2249 1884 1356 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2601 2915 3051 3226 3588 3998 Size: 393 432 537 314 136 175 362 410 Cuts at: 3998 4739 4796 5076 5366 6554 7151 7938 8206 Size: 741 57 280 290 1188 597 787 268 Cuts at: 8206 1239 Size: 1524 Fragments arranged by size: 1524 1188 787 741 597 537 432 410 393 362 314 290 280 268 175 136 57 EcoO109I rG'GnC_Cy Cuts at: 2874 3310 2874 Size: 436 8055 EcoRI G'AATT_C Cuts at: 5800 6178 5800 Size: 378 8113 EcoRV GAT'ATC Cuts at: 4591 4591 Size: 8491 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3179 3568 4744 5140 3179 Size: 389 1176 396 6530 Fragments arranged by size: 6530 1176 396 389 FspI TGC'GCA Cuts at: 4881 6997 4881 Size: 2116 6375 HaeII r_GCGC'y Cuts at: 1678 2916 7638 1678 Size: 1238 4722 2531 Fragments arranged by size: 4722 2531 1238 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 2665 2697 2754 Size: 32 135 32 812 32 249 32 57 Cuts at: 2754 2786 3471 3478 3503 3510 4580 4612 7132 Size: 32 685 7 25 7 1070 32 2520 Cuts at: 7132 7164 7206 7238 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2520 1070 812 685 249 135 57 42 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2242 2932 3514 5835 6081 669 Size: 988 281 304 690 582 2321 246 3079 Fragments arranged by size: 3079 2321 988 690 582 304 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8491 HpaI GTT'AAC Cuts at: 6081 6081 Size: 8491 KpnI G_GTAC'C Cuts at: 5816 5816 Size: 8491 MfeI C'AATT_G Cuts at: 2370 4198 6090 2370 Size: 1828 1892 4771 Fragments arranged by size: 4771 1892 1828 MluI A'CGCG_T Cuts at: 8367 8367 Size: 8491 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2598 5042 5310 7605 7789 1305 Size: 1293 2444 268 2295 184 2007 Fragments arranged by size: 2444 2295 2007 1293 268 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2330 3728 3798 10 Size: 54 1425 343 498 1398 70 4703 Fragments arranged by size: 4703 1425 1398 498 343 70 54 MslI CAynn'nnrTG Cuts at: 518 1166 2226 2777 4805 4899 5557 5729 6449 Size: 648 1060 551 2028 94 658 172 720 Cuts at: 6449 6808 6967 518 Size: 359 159 2042 Fragments arranged by size: 2042 2028 1060 720 658 648 551 359 172 159 94 MspA1I CmG'CkG Cuts at: 1606 3246 5907 6584 7732 7977 1606 Size: 1640 2661 677 1148 245 2120 Fragments arranged by size: 2661 2120 1640 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2266 3968 513 Size: 552 1201 1702 5036 Fragments arranged by size: 5036 1702 1201 552 NdeI CA'TA_TG Cuts at: 387 5248 387 Size: 4861 3630 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8491 NotI GC'GGCC_GC Cuts at: 5938 5938 Size: 8491 NsiI A_TGCA'T Cuts at: 3446 3446 Size: 8491 NspI r_CATG'y Cuts at: 1132 1229 2559 3604 5849 7394 1132 Size: 97 1330 1045 2245 1545 2229 Fragments arranged by size: 2245 2229 1545 1330 1045 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8491 PciI A'CATG_T Cuts at: 7390 7390 Size: 8491 PflMI CCAn_nnn'nTGG Cuts at: 1108 2685 3702 4945 1108 Size: 1577 1017 1243 4654 Fragments arranged by size: 4654 1577 1243 1017 PfoI T'CCnGG_A Cuts at: 5301 5301 Size: 8491 PmeI GTTT'AAAC Cuts at: 5795 5795 Size: 8491 PmlI CAC'GTG Cuts at: 3362 3362 Size: 8491 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6560 6592 8073 8105 6560 Size: 32 1481 32 6946 Fragments arranged by size: 6946 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3310 3310 Size: 8491 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8491 PsiI TTA'TAA Cuts at: 2598 6061 2598 Size: 3463 5028 PstI C_TGCA'G Cuts at: 830 3667 5473 5843 830 Size: 2837 1806 370 3478 Fragments arranged by size: 3478 2837 1806 370 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8491 PvuII CAG'CTG Cuts at: 3246 3246 Size: 8491 SalI G'TCGA_C Cuts at: 1936 5833 1936 Size: 3897 4594 SanDI GG'GwC_CC Cuts at: 3310 3310 Size: 8491 SbfI CC_TGCA'GG Cuts at: 5843 5843 Size: 8491 ScaI AGT'ACT Cuts at: 5506 6739 8451 5506 Size: 1233 1712 5546 Fragments arranged by size: 5546 1712 1233 SfcI C'TryA_G Cuts at: 826 1045 3663 4650 5469 5531 5839 6974 7655 Size: 219 2618 987 819 62 308 1135 681 Cuts at: 7655 7846 826 Size: 191 1471 Fragments arranged by size: 2618 1471 1135 987 819 681 308 219 191 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8491 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8491 SmaI CCC'GGG Cuts at: 1872 5818 1872 Size: 3946 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3950 6595 7496 7758 820 Size: 197 926 2007 2645 901 262 1553 Fragments arranged by size: 2645 2007 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6282 493 Size: 5789 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8491 SphI G_CATG'C Cuts at: 1132 3604 5849 1132 Size: 2472 2245 3774 Fragments arranged by size: 3774 2472 2245 SspI AAT'ATT Cuts at: 5 52 6415 5 Size: 47 6363 2081 Fragments arranged by size: 6363 2081 47 StuI AGG'CCT Cuts at: 1517 2514 1517 Size: 997 7494 StyI C'CwwG_G Cuts at: 513 1065 2266 3968 5626 5649 5930 513 Size: 552 1201 1702 1658 23 281 3074 Fragments arranged by size: 3074 1702 1658 1201 552 281 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4530 5636 6518 6535 6688 6873 7318 Size: 1255 2950 1106 882 17 153 185 445 Cuts at: 7318 325 Size: 1498 Fragments arranged by size: 2950 1498 1255 1106 882 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2868 3958 4848 Size: 274 80 33 51 1443 891 1090 890 Cuts at: 4848 5037 5504 6737 8449 96 Size: 189 467 1233 1712 138 Fragments arranged by size: 1712 1443 1233 1090 891 890 467 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3096 3128 8451 8483 3096 Size: 32 5323 32 3104 Fragments arranged by size: 5323 3104 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8491 XbaI T'CTAG_A Cuts at: 5827 5827 Size: 8491 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8491 XmaI C'CCGG_G Cuts at: 1870 5816 1870 Size: 3946 4545 XmnI GAAnn'nnTTC Cuts at: 2838 6620 2838 Size: 3782 4709 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3946 4746 6299 276 Size: 53 83 186 936 2412 800 1553 2468 Fragments arranged by size: 2468 2412 1553 936 800 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI BsmBI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI