(Circular) (Six-base) MAPSORT of: pFN21AB9886.seq Check: 6669 from: 1 to: 6880 pFN21AB9886 6880 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3514 3514 Size: 6880 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4690 278 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2193 4223 1937 Size: 256 2030 4594 Fragments arranged by size: 4594 2030 256 Acc65I G'GTAC_C Cuts at: 4201 4201 Size: 6880 AclI AA'CG_TT Cuts at: 1563 5007 5380 1563 Size: 3444 373 3063 Fragments arranged by size: 3444 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 3048 3738 3920 4943 6327 6595 1632 Size: 569 847 690 182 1023 1384 268 1917 Fragments arranged by size: 1917 1384 1023 847 690 569 268 182 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6683 AflIII A'CryG_T Cuts at: 1203 2751 5779 6756 1203 Size: 1548 3028 977 1327 Fragments arranged by size: 3028 1548 1327 977 AgeI A'CCGG_T Cuts at: 5711 5711 Size: 6880 AhdI GACnn_n'nnGTC Cuts at: 5609 5609 Size: 6880 AleI CACnn'nnGTG Cuts at: 1166 3179 1166 Size: 2013 4867 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2543 1184 Size: 1359 5521 AlwNI CAG_nnn'CTG Cuts at: 2155 2179 3317 3446 3456 3560 3598 4623 6195 Size: 24 1138 129 10 104 38 1025 1572 Cuts at: 6195 2155 Size: 2840 Fragments arranged by size: 2840 1572 1138 1025 129 104 38 24 10 ApaI G_GGCC'C Cuts at: 2854 2854 Size: 6880 ApaLI G'TGCA_C Cuts at: 2612 3274 4937 6093 2612 Size: 662 1663 1156 3399 Fragments arranged by size: 3399 1663 1156 662 ApoI r'AATT_y Cuts at: 1493 3422 4189 4415 4567 6676 1493 Size: 1929 767 226 152 2109 1697 Fragments arranged by size: 2109 1929 1697 767 226 152 AseI AT'TA_AT Cuts at: 160 1032 5434 160 Size: 872 4402 1606 Fragments arranged by size: 4402 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 AvaI C'yCGr_G Cuts at: 1870 1943 2432 2774 2898 3407 4205 1870 Size: 73 489 342 124 509 798 4545 Fragments arranged by size: 4545 798 509 489 342 124 73 BamHI G'GATC_C Cuts at: 3012 4003 4210 4577 3012 Size: 991 207 367 5315 Fragments arranged by size: 5315 991 367 207 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2438 3353 4201 5656 618 Size: 325 217 627 651 915 848 1455 1842 Fragments arranged by size: 1842 1455 915 848 651 627 325 217 BanII G_rGCy'C Cuts at: 1391 2854 4199 1391 Size: 1463 1345 4072 Fragments arranged by size: 4072 1463 1345 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3036 3382 3980 928 Size: 971 1137 346 598 3828 Fragments arranged by size: 3828 1137 971 598 346 BbvCI CC'TCA_GC Cuts at: 2661 2661 Size: 6880 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2023 2057 5071 5105 1903 Size: 34 86 34 3014 34 3678 Fragments arranged by size: 3678 3014 86 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4772 5982 4772 Size: 1210 5670 BclI T'GATC_A Cuts at: 1548 1548 Size: 6880 BfrBI ATG'CAT Cuts at: 4037 4133 4037 Size: 96 6784 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2415 2665 5491 Size: 107 122 71 631 605 743 250 2826 Cuts at: 5491 136 Size: 1525 Fragments arranged by size: 2826 1525 743 631 605 250 122 107 71 BglII A'GATC_T Cuts at: 2232 4120 6875 2232 Size: 1888 2755 2237 Fragments arranged by size: 2755 2237 1888 BlpI GC'TnA_GC Cuts at: 2264 4297 2264 Size: 2033 4847 Bme1580I G_kGCm'C Cuts at: 1790 2616 2854 3278 4941 6097 1790 Size: 826 238 424 1663 1156 2573 Fragments arranged by size: 2573 1663 1156 826 424 238 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6880 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3608 4611 5569 Size: 416 53 83 399 24 2191 1003 958 Cuts at: 5569 442 Size: 1753 Fragments arranged by size: 2191 1753 1003 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6880 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2417 3565 2417 Size: 1148 5732 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2111 2264 2318 2379 2552 2855 5540 Size: 836 36 153 54 61 173 303 2685 Cuts at: 5540 1239 Size: 2579 Fragments arranged by size: 2685 2579 836 303 173 153 61 54 36 Bpu10I CC'TnA_GC Cuts at: 2661 3579 2661 Size: 918 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2522 5005 5870 6168 2522 Size: 2483 865 298 3234 Fragments arranged by size: 3234 2483 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2388 5543 882 Size: 617 889 3155 2219 Fragments arranged by size: 3155 2219 889 617 BsaAI yAC'GTr Cuts at: 493 1204 2086 4671 493 Size: 711 882 2585 2702 Fragments arranged by size: 2702 2585 882 711 BsaBI GATnn'nnATC Cuts at: 6874 6874 Size: 6880 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2537 3749 4687 5069 Size: 53 83 186 936 1004 1212 938 382 Cuts at: 5069 5704 275 Size: 635 1451 Fragments arranged by size: 1451 1212 1004 938 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2456 5313 5711 5985 6132 6543 2456 Size: 2857 398 274 147 411 2793 Fragments arranged by size: 2857 2793 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4064 4094 1178 Size: 30 165 30 2661 30 3964 Fragments arranged by size: 3964 2661 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2381 2897 3170 3745 1186 Size: 991 204 516 273 575 4321 Fragments arranged by size: 4321 991 575 516 273 204 BseYI C'CCAG_C Cuts at: 3265 3831 6083 6579 3265 Size: 566 2252 496 3566 Fragments arranged by size: 3566 2252 566 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3905 1163 Size: 573 2169 4138 Fragments arranged by size: 4138 2169 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4330 5091 6119 1540 Size: 275 186 2329 761 1028 2301 Fragments arranged by size: 2329 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2136 2616 3278 4941 5026 6097 2136 Size: 480 662 1663 85 1071 2919 Fragments arranged by size: 2919 1663 1071 662 480 85 BsmI GAATG_Cn' Cuts at: 4039 4135 4391 4484 4039 Size: 96 256 93 6435 Fragments arranged by size: 6435 256 96 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2159 2213 2528 2159 Size: 54 315 6511 Fragments arranged by size: 6511 315 54 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2616 2854 3278 4199 4941 5026 Size: 399 346 480 238 424 921 742 85 Cuts at: 5026 6097 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 921 742 480 424 399 346 238 85 BspEI T'CCGG_A Cuts at: 6543 6543 Size: 6880 BspHI T'CATG_A Cuts at: 2625 4769 6499 2625 Size: 2144 1730 3006 Fragments arranged by size: 3006 2144 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3087 3514 4111 4235 844 Size: 2243 427 597 124 3489 Fragments arranged by size: 3489 2243 597 427 124 BsrBI CCG'CTC Cuts at: 1394 1823 4767 1394 Size: 429 2944 3507 Fragments arranged by size: 3507 2944 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3045 3586 5375 5549 65 Size: 1183 1797 541 1789 174 1396 Fragments arranged by size: 1797 1789 1396 1183 541 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2136 2205 2414 5524 5711 1700 Size: 110 326 69 209 3110 187 2869 Fragments arranged by size: 3110 2869 326 209 187 110 69 BsrGI T'GTAC_A Cuts at: 96 2556 96 Size: 2460 4420 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6880 BssSI C'ACGA_G Cuts at: 4940 5952 4940 Size: 1012 5868 BstAPI GCAn_nnn'nTGC Cuts at: 794 2611 794 Size: 1817 5063 BstBI TT'CG_AA Cuts at: 3192 5684 3192 Size: 2492 4388 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6880 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6880 BstYI r'GATC_y Cuts at: 1972 2232 3012 3616 4003 4120 4210 4577 4962 Size: 260 780 604 387 117 90 367 385 Cuts at: 4962 4979 6431 6517 6529 6875 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 780 604 387 385 367 346 260 117 90 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2973 2973 Size: 6880 BtgI C'CryG_G Cuts at: 513 1065 2004 3003 3492 3876 513 Size: 552 939 999 489 384 3517 Fragments arranged by size: 3517 999 939 552 489 384 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2024 2030 3692 487 Size: 683 56 798 6 1662 3675 Fragments arranged by size: 3675 1662 798 683 56 6 BtsI GCAGTG_nn' Cuts at: 4396 5190 5210 4396 Size: 794 20 6066 Fragments arranged by size: 6066 794 20 ClaI AT'CG_AT Cuts at: 1554 3432 1554 Size: 1878 5002 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2482 2517 4155 4190 529 Size: 35 1918 35 1638 35 3219 Fragments arranged by size: 3219 1918 1638 35 35 35 DraI TTT'AAA Cuts at: 4184 4184 Size: 6880 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3038 5887 809 Size: 507 1722 2849 1802 Fragments arranged by size: 2849 1802 1722 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2712 4327 5216 Size: 54 1425 50 275 18 882 1615 889 Cuts at: 5216 6845 8 Size: 1629 43 Fragments arranged by size: 1629 1615 1425 889 882 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4327 1537 Size: 275 2515 4090 Fragments arranged by size: 4090 2515 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2979 3714 3771 3885 4810 1353 Size: 1626 735 57 114 925 3423 Fragments arranged by size: 3423 1626 925 735 114 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 5467 5841 5987 387 Size: 1064 138 585 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 585 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2111 2201 2264 2318 2379 2552 Size: 393 443 36 90 63 54 61 173 Cuts at: 2552 2855 3048 3738 3920 4943 5540 6327 6595 Size: 303 193 690 182 1023 597 787 268 Cuts at: 6595 1239 Size: 1524 Fragments arranged by size: 1524 1023 787 690 597 443 393 303 268 193 182 173 90 63 61 54 36 EcoO109I rG'GnC_Cy Cuts at: 2122 2566 2850 2851 3663 3895 2122 Size: 444 284 1 812 232 5107 Fragments arranged by size: 5107 812 444 284 232 1 EcoRI G'AATT_C Cuts at: 4189 4567 4189 Size: 378 6502 EcoRV GAT'ATC Cuts at: 3607 3607 Size: 6880 FspI TGC'GCA Cuts at: 3051 5386 3051 Size: 2335 4545 HaeII r_GCGC'y Cuts at: 1678 2156 6027 1678 Size: 478 3871 2531 Fragments arranged by size: 3871 2531 478 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3208 3240 3603 3635 5521 Size: 32 135 32 1636 32 363 32 1886 Cuts at: 5521 5553 5595 5627 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1886 1636 363 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3076 4224 4470 669 Size: 988 281 1138 1148 246 3079 Fragments arranged by size: 3079 1148 1138 988 281 246 HindIII A'AGCT_T Cuts at: 748 4017 748 Size: 3269 3611 HpaI GTT'AAC Cuts at: 4470 4470 Size: 6880 KpnI G_GTAC'C Cuts at: 4205 4205 Size: 6880 MfeI C'AATT_G Cuts at: 4479 4479 Size: 6880 MluI A'CGCG_T Cuts at: 6756 6756 Size: 6880 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2001 2088 5994 6178 1305 Size: 696 87 3906 184 2007 Fragments arranged by size: 3906 2007 696 184 87 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5058 Fragments arranged by size: 5058 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3179 3497 3503 4838 5197 5356 Size: 648 843 1170 318 6 1335 359 159 Cuts at: 5356 518 Size: 2042 Fragments arranged by size: 2042 1335 1170 843 648 359 318 159 6 MspA1I CmG'CkG Cuts at: 1606 2053 2330 2608 2665 2891 2966 3317 3453 Size: 447 277 278 57 226 75 351 136 Cuts at: 3453 3560 4296 4973 6121 6366 1606 Size: 107 736 677 1148 245 2120 Fragments arranged by size: 2120 1148 736 677 447 351 278 277 245 226 136 107 75 57 NaeI GCC'GGC Cuts at: 2416 2416 Size: 6880 NcoI C'CATG_G Cuts at: 513 1065 2004 3003 3492 3876 513 Size: 552 939 999 489 384 3517 Fragments arranged by size: 3517 999 939 552 489 384 NdeI CA'TA_TG Cuts at: 387 387 Size: 6880 NgoMIV G'CCGG_C Cuts at: 2414 2414 Size: 6880 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6880 NotI GC'GGCC_GC Cuts at: 4327 4327 Size: 6880 NsiI A_TGCA'T Cuts at: 4039 4135 4039 Size: 96 6784 NspI r_CATG'y Cuts at: 1132 1229 2755 3502 3977 4238 5783 1132 Size: 97 1526 747 475 261 1545 2229 Fragments arranged by size: 2229 1545 1526 747 475 261 97 PasI CC'CwG_GG Cuts at: 1348 3760 1348 Size: 2412 4468 PciI A'CATG_T Cuts at: 2751 5779 2751 Size: 3028 3852 PflMI CCAn_nnn'nTGG Cuts at: 1108 2155 3009 3103 3598 1108 Size: 1047 854 94 495 4390 Fragments arranged by size: 4390 1047 854 495 94 PfoI T'CCnGG_A Cuts at: 2127 3015 2127 Size: 888 5992 PmeI GTTT'AAAC Cuts at: 4184 4184 Size: 6880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4949 4981 6462 6494 4949 Size: 32 1481 32 5335 Fragments arranged by size: 5335 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 2566 2122 Size: 444 6436 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6880 PsiI TTA'TAA Cuts at: 4450 4450 Size: 6880 PspOMI G'GGCC_C Cuts at: 2850 2850 Size: 6880 PspXI vC'TCGA_Gb Cuts at: 2898 2898 Size: 6880 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3830 3862 3830 Size: 32 6848 PstI C_TGCA'G Cuts at: 830 2522 2607 4232 830 Size: 1692 85 1625 3478 Fragments arranged by size: 3478 1692 1625 85 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6880 PvuII CAG'CTG Cuts at: 2053 2966 3560 2053 Size: 913 594 5373 Fragments arranged by size: 5373 913 594 SalI G'TCGA_C Cuts at: 1936 4222 1936 Size: 2286 4594 SbfI CC_TGCA'GG Cuts at: 4232 4232 Size: 6880 ScaI AGT'ACT Cuts at: 2549 5128 6840 2549 Size: 2579 1712 2589 Fragments arranged by size: 2589 2579 1712 SfcI C'TryA_G Cuts at: 826 1045 2518 2603 3552 4228 5363 6044 6235 Size: 219 1473 85 949 676 1135 681 191 Cuts at: 6235 826 Size: 1471 Fragments arranged by size: 1473 1471 1135 949 681 676 219 191 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6880 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6880 SmaI CCC'GGG Cuts at: 1872 2434 4207 1872 Size: 562 1773 4545 Fragments arranged by size: 4545 1773 562 SmlI C'TyrA_G Cuts at: 820 1017 1943 2501 2898 3407 4984 5885 6147 Size: 197 926 558 397 509 1577 901 262 Cuts at: 6147 820 Size: 1553 Fragments arranged by size: 1577 1553 926 901 558 509 397 262 197 SnaBI TAC'GTA Cuts at: 493 4671 493 Size: 4178 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6880 SphI G_CATG'C Cuts at: 1132 4238 1132 Size: 3106 3774 SspI AAT'ATT Cuts at: 5 52 4804 5 Size: 47 4752 2081 Fragments arranged by size: 4752 2081 47 StuI AGG'CCT Cuts at: 1517 3284 1517 Size: 1767 5113 StyI C'CwwG_G Cuts at: 513 1065 2004 2922 3003 3492 3658 3876 4319 Size: 552 939 918 81 489 166 218 443 Cuts at: 4319 513 Size: 3074 Fragments arranged by size: 3074 939 918 552 489 443 218 166 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2294 3590 3823 4907 4924 5077 5262 Size: 1255 714 1296 233 1084 17 153 185 Cuts at: 5262 5707 325 Size: 445 1498 Fragments arranged by size: 1498 1296 1255 1084 714 445 233 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 2556 3813 Size: 274 80 33 51 1443 570 9 1257 Cuts at: 3813 4163 5126 6838 96 Size: 350 963 1712 138 Fragments arranged by size: 1712 1443 1257 963 570 350 274 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6840 6872 6840 Size: 32 6848 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6880 XbaI T'CTAG_A Cuts at: 4136 4216 4136 Size: 80 6800 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3874 3883 3874 Size: 9 6871 XhoI C'TCGA_G Cuts at: 1943 2898 3407 1943 Size: 955 509 5416 Fragments arranged by size: 5416 955 509 XmaI C'CCGG_G Cuts at: 1870 2432 4205 1870 Size: 562 1773 4545 Fragments arranged by size: 4545 1773 562 XmnI GAAnn'nnTTC Cuts at: 5009 5009 Size: 6880 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4688 276 Size: 53 83 186 936 3154 2468 Fragments arranged by size: 3154 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI PacI PmlI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI