(Circular) (Six-base) MAPSORT of: pFN21AB3065.seq Check: 9609 from: 1 to: 6121 pFN21AB3065 6121 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2517 3464 1937 Size: 580 947 4594 Fragments arranged by size: 4594 947 580 Acc65I G'GTAC_C Cuts at: 3442 3442 Size: 6121 AclI AA'CG_TT Cuts at: 1563 4248 4621 1563 Size: 2685 373 3063 Fragments arranged by size: 3063 2685 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4184 5568 5836 1632 Size: 2552 1384 268 1917 Fragments arranged by size: 2552 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 2538 2565 2886 2964 3402 5020 5997 1203 Size: 1335 27 321 78 438 1618 977 1327 Fragments arranged by size: 1618 1335 1327 977 438 321 78 27 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 4850 4850 Size: 6121 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3364 1184 Size: 2180 3941 AlwNI CAG_nnn'CTG Cuts at: 2353 3268 3864 5436 2353 Size: 915 596 1572 3038 Fragments arranged by size: 3038 1572 915 596 ApaI G_GGCC'C Cuts at: 3077 3077 Size: 6121 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 2144 2243 3430 3656 3808 5917 1493 Size: 651 99 1187 226 152 2109 1697 Fragments arranged by size: 2109 1697 1187 651 226 152 99 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 3446 1870 Size: 73 1503 4545 Fragments arranged by size: 4545 1503 73 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2384 2939 3442 4897 618 Size: 325 217 627 597 555 503 1455 1842 Fragments arranged by size: 1842 1455 627 597 555 503 325 217 BanII G_rGCy'C Cuts at: 1391 2078 3077 3440 1391 Size: 687 999 363 4072 Fragments arranged by size: 4072 999 687 363 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2103 928 Size: 971 204 4946 Fragments arranged by size: 4946 971 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 1903 Size: 34 2375 34 3678 Fragments arranged by size: 3678 2375 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 5223 4013 Size: 1210 4911 BclI T'GATC_A Cuts at: 1548 3060 1548 Size: 1512 4609 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2708 4732 136 Size: 107 122 71 631 605 1036 2024 1525 Fragments arranged by size: 2024 1525 1036 631 605 122 107 71 BglII A'GATC_T Cuts at: 6116 6116 Size: 6121 BlpI GC'TnA_GC Cuts at: 3538 3538 Size: 6121 Bme1580I G_kGCm'C Cuts at: 1790 2387 3077 4182 5338 1790 Size: 597 690 1105 1156 2573 Fragments arranged by size: 2573 1156 1105 690 597 BmgBI CAC'GTC Cuts at: 1318 2967 3405 1318 Size: 1649 438 4034 Fragments arranged by size: 4034 1649 438 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2591 3852 4810 Size: 416 53 83 399 24 1174 1261 958 Cuts at: 4810 442 Size: 1753 Fragments arranged by size: 1753 1261 1174 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 4781 1239 Size: 1181 2361 2579 Fragments arranged by size: 2579 2361 1181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4246 5111 5409 4246 Size: 865 298 4958 Fragments arranged by size: 4958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4784 882 Size: 617 3285 2219 Fragments arranged by size: 3285 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2071 2539 3912 493 Size: 711 867 468 1373 2702 Fragments arranged by size: 2702 1373 867 711 468 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3928 4310 4945 275 Size: 53 83 186 936 2395 382 635 1451 Fragments arranged by size: 2395 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3228 4554 4952 5226 5373 5784 3228 Size: 1326 398 274 147 411 3565 Fragments arranged by size: 3565 1326 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5896 Fragments arranged by size: 5896 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 3217 1186 Size: 1111 920 4090 Fragments arranged by size: 4090 1111 920 BseYI C'CCAG_C Cuts at: 2208 2218 2342 2380 3069 5324 5820 2208 Size: 10 124 38 689 2255 496 2509 Fragments arranged by size: 2509 2255 689 496 124 38 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3284 1163 Size: 573 1548 4000 Fragments arranged by size: 4000 1548 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3571 4332 5360 1540 Size: 275 186 1570 761 1028 2301 Fragments arranged by size: 2301 1570 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3362 4182 4267 5338 3362 Size: 820 85 1071 4145 Fragments arranged by size: 4145 1071 820 85 BsmI GAATG_Cn' Cuts at: 3143 3632 3725 3143 Size: 489 93 5539 Fragments arranged by size: 5539 489 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2387 3077 3362 3440 4182 4267 Size: 399 288 309 690 285 78 742 85 Cuts at: 4267 5338 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 690 399 309 288 285 85 78 BspEI T'CCGG_A Cuts at: 3228 5784 3228 Size: 2556 3565 BspHI T'CATG_A Cuts at: 4010 5740 4010 Size: 1730 4391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3222 3476 844 Size: 2378 254 3489 Fragments arranged by size: 3489 2378 254 BsrBI CCG'CTC Cuts at: 1394 1823 4008 1394 Size: 429 2185 3507 Fragments arranged by size: 3507 2185 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2358 4616 4790 65 Size: 1183 1110 2258 174 1396 Fragments arranged by size: 2258 1396 1183 1110 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4765 4952 1700 Size: 110 2955 187 2869 Fragments arranged by size: 2955 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 3317 794 Size: 2523 3598 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 2848 2989 3379 1184 Size: 1664 141 390 3926 Fragments arranged by size: 3926 1664 390 141 BstXI CCAn_nnnn'nTGG Cuts at: 2704 3037 2704 Size: 333 5788 BstYI r'GATC_y Cuts at: 1972 3451 3818 4203 4220 5672 5758 5770 6116 Size: 1479 367 385 17 1452 86 12 346 Cuts at: 6116 1972 Size: 1977 Fragments arranged by size: 1977 1479 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2613 2703 513 Size: 552 1548 90 3931 Fragments arranged by size: 3931 1548 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 2604 3255 3637 4431 4451 2604 Size: 651 382 794 20 4274 Fragments arranged by size: 4274 794 651 382 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2566 2601 529 Size: 35 2002 35 4049 Fragments arranged by size: 4049 2002 35 35 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2355 2996 3568 Size: 54 1425 50 275 18 525 641 572 Cuts at: 3568 4457 6086 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 641 572 525 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2942 4051 1353 Size: 1589 1109 3423 Fragments arranged by size: 3423 1589 1109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 4184 4781 5568 5836 1239 Size: 393 788 1764 597 787 268 1524 Fragments arranged by size: 1764 1524 788 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2677 2677 Size: 6121 EcoO109I rG'GnC_Cy Cuts at: 2664 3073 3074 3211 2664 Size: 409 1 137 5574 Fragments arranged by size: 5574 409 137 1 EcoRI G'AATT_C Cuts at: 3430 3808 3430 Size: 378 5743 FspI TGC'GCA Cuts at: 3261 4627 3261 Size: 1366 4755 HaeII r_GCGC'y Cuts at: 1678 5268 1678 Size: 3590 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 4762 4794 4836 Size: 32 135 32 530 32 2628 32 42 Cuts at: 4836 4868 1373 Size: 32 2626 Fragments arranged by size: 2628 2626 530 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3465 3711 669 Size: 988 281 1527 246 3079 Fragments arranged by size: 3079 1527 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 3446 3446 Size: 6121 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5235 5419 1305 Size: 3930 184 2007 Fragments arranged by size: 3930 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2357 10 Size: 54 1425 343 525 3774 Fragments arranged by size: 3774 1425 525 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2817 3389 4079 4438 4597 518 Size: 648 1651 572 690 359 159 2042 Fragments arranged by size: 2042 1651 690 648 572 359 159 MspA1I CmG'CkG Cuts at: 1606 2353 2448 3537 4214 5362 5607 1606 Size: 747 95 1089 677 1148 245 2120 Fragments arranged by size: 2120 1148 1089 747 677 245 95 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5569 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 2890 3238 3479 5024 1132 Size: 97 1661 348 241 1545 2229 Fragments arranged by size: 2229 1661 1545 348 241 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6121 PciI A'CATG_T Cuts at: 2886 5020 2886 Size: 2134 3987 PflMI CCAn_nnn'nTGG Cuts at: 1108 2353 1108 Size: 1245 4876 PfoI T'CCnGG_A Cuts at: 2397 2397 Size: 6121 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PmlI CAC'GTG Cuts at: 2539 2539 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3211 3211 Size: 6121 PshAI GACnn'nnGTC Cuts at: 1613 3038 1613 Size: 1425 4696 PsiI TTA'TAA Cuts at: 3691 3691 Size: 6121 PspOMI G'GGCC_C Cuts at: 3073 3073 Size: 6121 PstI C_TGCA'G Cuts at: 830 2453 3152 3473 830 Size: 1623 699 321 3478 Fragments arranged by size: 3478 1623 699 321 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 2353 2353 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SapI GCTCTTCn'nnn_ Cuts at: 2942 2942 Size: 6121 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SexAI A'CCwGG_T Cuts at: 2226 2226 Size: 6121 SfcI C'TryA_G Cuts at: 826 1045 2449 2518 3148 3469 4604 5285 5476 Size: 219 1404 69 630 321 1135 681 191 Cuts at: 5476 826 Size: 1471 Fragments arranged by size: 1471 1404 1135 681 630 321 219 191 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 3448 1872 Size: 1576 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4225 5126 5388 820 Size: 197 926 2282 901 262 1553 Fragments arranged by size: 2282 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 3479 1132 Size: 2347 3774 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 2455 1517 Size: 938 5183 StyI C'CwwG_G Cuts at: 513 1065 2697 3375 3560 513 Size: 552 1632 678 185 3074 Fragments arranged by size: 3074 1632 678 552 185 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2573 2957 4148 4165 4318 4503 4948 Size: 1255 993 384 1191 17 153 185 445 Cuts at: 4948 325 Size: 1498 Fragments arranged by size: 1498 1255 1191 993 445 384 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4367 6079 96 Size: 274 80 33 51 1443 2390 1712 138 Fragments arranged by size: 2390 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2130 2162 2306 2338 6081 6113 2130 Size: 32 144 32 3743 32 2138 Fragments arranged by size: 3743 2138 144 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2704 2350 Size: 354 5767 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 3446 1870 Size: 1576 4545 XmnI GAAnn'nnTTC Cuts at: 4250 4250 Size: 6121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI