Restriction map for
pFN21AB9466
(Linear) (Six-base) MAPSORT of: KIBB9466.seq Check: 414 from: 1 to: 2331
KIBB9466.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 0 394 550 2331
Size: 394 156 1781
Fragments arranged by size:
1781 394 156
AclI AA'CG_TT
Cuts at: 0 1570 2331
Size: 1570 761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1268 1429 1643 1951 2003 2059 2331
Size: 476 792 161 214 308 52 56 272
Fragments arranged by size:
792 476 308 272 214 161 56 52
AflIII A'CryG_T
Cuts at: 0 1691 2331
Size: 1691 640
AleI CACnn'nnGTG
Cuts at: 0 436 2331
Size: 436 1895
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1730 2331
Size: 1730 601
ApoI r'AATT_y
Cuts at: 0 1400 1496 2242 2331
Size: 1400 96 746 89
Fragments arranged by size:
1400 746 96 89
AseI AT'TA_AT
Cuts at: 0 1886 2331
Size: 1886 445
AvaI C'yCGr_G
Cuts at: 0 339 2248 2331
Size: 339 1909 83
Fragments arranged by size:
1909 339 83
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 976 1009 2331
Size: 976 33 1322
Fragments arranged by size:
1322 976 33
BanI G'GyrC_C
Cuts at: 0 523 1097 2331
Size: 523 574 1234
Fragments arranged by size:
1234 574 523
BbsI GAAGACnn'nnnn_
Cuts at: 0 122 183 698 1435 2331
Size: 122 61 515 737 896
Fragments arranged by size:
896 737 515 122 61
BfrBI ATG'CAT
Cuts at: 0 2175 2331
Size: 2175 156
BglI GCCn_nnn'nGGC
Cuts at: 0 521 2331
Size: 521 1810
BglII A'GATC_T
Cuts at: 0 228 783 2331
Size: 228 555 1548
Fragments arranged by size:
1548 555 228
Bme1580I G_kGCm'C
Cuts at: 0 895 2331
Size: 895 1436
BmrI ACTGGGnnnn_n'
Cuts at: 0 797 898 2331
Size: 797 101 1433
Fragments arranged by size:
1433 797 101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1919 2331
Size: 1919 412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1001 1130 2106 2331
Size: 941 60 129 976 225
Fragments arranged by size:
976 941 225 129 60
Bpu10I CC'TnA_GC
Cuts at: 0 59 2331
Size: 59 2272
BsaI GGTCTCn'nnnn_
Cuts at: 0 1188 2331
Size: 1188 1143
BsaWI w'CCGG_w
Cuts at: 0 750 2331
Size: 750 1581
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 98 128 773 803 2331
Size: 98 30 645 30 1528
Fragments arranged by size:
1528 645 98 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 116 119 668 1202 1215 2331
Size: 116 3 549 534 13 1116
Fragments arranged by size:
1116 549 534 116 13 3
BseYI C'CCAG_C
Cuts at: 0 43 1186 1419 2331
Size: 43 1143 233 912
Fragments arranged by size:
1143 912 233 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 2331
Size: 843 1488
BsiHKAI G_wGCw'C
Cuts at: 0 1273 2331
Size: 1273 1058
BsmBI CGTCTCn'nnnn_
Cuts at: 0 613 2331
Size: 613 1718
Bsp1286I G_dGCh'C
Cuts at: 0 895 1273 2331
Size: 895 378 1058
Fragments arranged by size:
1058 895 378
BspHI T'CATG_A
Cuts at: 0 1676 2331
Size: 1676 655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1865 2331
Size: 1865 466
BsrDI GCAATG_nn'
Cuts at: 0 219 374 543 2331
Size: 219 155 169 1788
Fragments arranged by size:
1788 219 169 155
BstAPI GCAn_nnn'nTGC
Cuts at: 0 433 2331
Size: 433 1898
BstBI TT'CG_AA
Cuts at: 0 1404 2331
Size: 1404 927
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1108 2331
Size: 1108 1223
BstYI r'GATC_y
Cuts at: 0 228 783 1105 2331
Size: 228 555 322 1226
Fragments arranged by size:
1226 555 322 228
BstZ17I GTA'TAC
Cuts at: 0 551 2331
Size: 551 1780
Bsu36I CC'TnA_GG
Cuts at: 0 2027 2331
Size: 2027 304
BtsI GCAGTG_nn'
Cuts at: 0 54 431 814 1349 1727 2331
Size: 54 377 383 535 378 604
Fragments arranged by size:
604 535 383 378 377 54
ClaI AT'CG_AT
Cuts at: 0 1394 2331
Size: 1394 937
EarI CTCTTCn'nnn_
Cuts at: 0 1976 2022 2035 2331
Size: 1976 46 13 296
Fragments arranged by size:
1976 296 46 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 941 1001 1130 1268 1429 1643 1951
Size: 476 465 60 129 138 161 214 308
Cuts at: 1951 2003 2059 2106 2331
Size: 52 56 47 225
Fragments arranged by size:
476 465 308 225 214 161 138 129
60 56 52 47
EcoO109I rG'GnC_Cy
Cuts at: 0 605 2331
Size: 605 1726
EcoRI G'AATT_C
Cuts at: 0 1496 2242 2331
Size: 1496 746 89
Fragments arranged by size:
1496 746 89
FspI TGC'GCA
Cuts at: 0 218 2331
Size: 218 2113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1192 1224 2331
Size: 1192 32 1107
Fragments arranged by size:
1192 1107 32
HincII GTy'rAC
Cuts at: 0 395 2331
Size: 395 1936
HindIII A'AGCT_T
Cuts at: 0 358 912 1280 2331
Size: 358 554 368 1051
Fragments arranged by size:
1051 554 368 358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1586 2331
Size: 1586 745
MslI CAynn'nnrTG
Cuts at: 0 436 482 1766 2331
Size: 436 46 1284 565
Fragments arranged by size:
1284 565 436 46
MspA1I CmG'CkG
Cuts at: 0 2139 2331
Size: 2139 192
NsiI A_TGCA'T
Cuts at: 0 2177 2331
Size: 2177 154
NspI r_CATG'y
Cuts at: 0 1427 1695 1917 1938 2331
Size: 1427 268 222 21 393
Fragments arranged by size:
1427 393 268 222 21
PasI CC'CwG_GG
Cuts at: 0 865 2331
Size: 865 1466
PciI A'CATG_T
Cuts at: 0 1691 2331
Size: 1691 640
PfoI T'CCnGG_A
Cuts at: 0 918 1795 2331
Size: 918 877 536
Fragments arranged by size:
918 877 536
PpuMI rG'GwC_Cy
Cuts at: 0 605 2331
Size: 605 1726
PstI C_TGCA'G
Cuts at: 0 1790 2331
Size: 1790 541
SalI G'TCGA_C
Cuts at: 0 393 2331
Size: 393 1938
SanDI GG'GwC_CC
Cuts at: 0 605 2331
Size: 605 1726
SapI GCTCTTCn'nnn_
Cuts at: 0 2035 2331
Size: 2035 296
ScaI AGT'ACT
Cuts at: 0 1792 2331
Size: 1792 539
SexAI A'CCwGG_T
Cuts at: 0 1743 2331
Size: 1743 588
SfcI C'TryA_G
Cuts at: 0 1511 1786 2331
Size: 1511 275 545
Fragments arranged by size:
1511 545 275
SphI G_CATG'C
Cuts at: 0 1427 2331
Size: 1427 904
SspI AAT'ATT
Cuts at: 0 2290 2331
Size: 2290 41
StyI C'CwwG_G
Cuts at: 0 634 1101 2331
Size: 634 467 1230
Fragments arranged by size:
1230 634 467
TatI w'GTAC_w
Cuts at: 0 1790 2331
Size: 1790 541
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 98 130 2331
Size: 98 32 2201
Fragments arranged by size:
2201 98 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1260 1664 2331
Size: 1260 404 667
Fragments arranged by size:
1260 667 404
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AlfI ApoI AseI
AvaI BaeI BanI BbsI BfrBI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI
EarI Eco57MI EcoO109I EcoRI FspI Hin4I HincII HindIII
MmeI MslI MspA1I NsiI NspI PasI PciI PfoI
PpuMI PstI SalI SanDI SapI ScaI SexAI SfcI
SphI SspI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AsiSI AvrII BamHI BanII
BbeI BbvCI BcgI BciVI BclI BlpI BmgBI BmtI
BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstEII BtgI BtgZI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRV FalI FseI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SbfI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI StuI SwaI TaqII
Tth111I XbaI XhoI XmaI XmnI ZraI