(Circular) (Six-base) MAPSORT of: pFN21AB9104.seq Check: 478 from: 1 to: 6328 pFN21AB9104 6328 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3204 4138 278 Size: 53 83 186 936 1668 934 2468 Fragments arranged by size: 2468 1668 936 934 186 83 53 AccI GT'mk_AC Cuts at: 1937 3671 1937 Size: 1734 4594 Acc65I G'GTAC_C Cuts at: 3649 3649 Size: 6328 AclI AA'CG_TT Cuts at: 1563 2432 4455 4828 1563 Size: 869 2023 373 3063 Fragments arranged by size: 3063 2023 869 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3089 4391 5775 6043 1632 Size: 1457 1302 1384 268 1917 Fragments arranged by size: 1917 1457 1384 1302 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6131 AflIII A'CryG_T Cuts at: 1203 3257 5227 6204 1203 Size: 2054 1970 977 1327 Fragments arranged by size: 2054 1970 1327 977 AgeI A'CCGG_T Cuts at: 5159 5159 Size: 6328 AhdI GACnn_n'nnGTC Cuts at: 5057 5057 Size: 6328 AleI CACnn'nnGTG Cuts at: 1166 3113 1166 Size: 1947 4381 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3131 1184 Size: 1947 4381 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2336 2368 2336 Size: 32 6296 AlwNI CAG_nnn'CTG Cuts at: 2833 4071 5643 2833 Size: 1238 1572 3518 Fragments arranged by size: 3518 1572 1238 ApaLI G'TGCA_C Cuts at: 4385 5541 4385 Size: 1156 5172 ApoI r'AATT_y Cuts at: 1493 3637 3863 4015 6124 1493 Size: 2144 226 152 2109 1697 Fragments arranged by size: 2144 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4882 160 Size: 872 3850 1606 Fragments arranged by size: 3850 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 AvaI C'yCGr_G Cuts at: 1870 1943 2688 3653 1870 Size: 73 745 965 4545 Fragments arranged by size: 4545 965 745 73 BamHI G'GATC_C Cuts at: 2030 3091 3658 4025 2030 Size: 1061 567 367 4333 Fragments arranged by size: 4333 1061 567 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3107 3115 3607 3649 5104 Size: 325 217 627 1320 8 492 42 1455 Cuts at: 5104 618 Size: 1842 Fragments arranged by size: 1842 1455 1320 627 492 325 217 42 8 BanII G_rGCy'C Cuts at: 1391 2265 2580 2637 2817 3046 3567 3600 3647 Size: 874 315 57 180 229 521 33 47 Cuts at: 3647 1391 Size: 4072 Fragments arranged by size: 4072 874 521 315 229 180 57 47 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5357 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4519 4553 1903 Size: 34 50 34 2498 34 3678 Fragments arranged by size: 3678 2498 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4220 5430 4220 Size: 1210 5118 BclI T'GATC_A Cuts at: 1548 1548 Size: 6328 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2051 2243 4939 Size: 107 122 71 631 605 379 192 2696 Cuts at: 4939 136 Size: 1525 Fragments arranged by size: 2696 1525 631 605 379 192 122 107 71 BglII A'GATC_T Cuts at: 6323 6323 Size: 6328 BlpI GC'TnA_GC Cuts at: 2217 2493 3745 2217 Size: 276 1252 4800 Fragments arranged by size: 4800 1252 276 Bme1580I G_kGCm'C Cuts at: 1790 3120 3513 4389 5545 1790 Size: 1330 393 876 1156 2573 Fragments arranged by size: 2573 1330 1156 876 393 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2034 3595 4059 Size: 416 53 83 399 24 617 1561 464 Cuts at: 4059 5017 442 Size: 958 1753 Fragments arranged by size: 1753 1561 958 617 464 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6328 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2714 2714 Size: 6328 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2171 2195 2240 2303 2732 2855 2891 Size: 917 15 24 45 63 429 123 36 Cuts at: 2891 3242 3443 3551 3566 4988 1239 Size: 351 201 108 15 1422 2579 Fragments arranged by size: 2579 1422 917 429 351 201 123 108 63 45 36 24 15 15 Bpu10I CC'TnA_GC Cuts at: 3045 3045 Size: 6328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2594 4453 5318 5616 2594 Size: 1859 865 298 3306 Fragments arranged by size: 3306 1859 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3386 4991 882 Size: 617 1887 1605 2219 Fragments arranged by size: 2219 1887 1605 617 BsaAI yAC'GTr Cuts at: 493 1204 3260 3364 4119 493 Size: 711 2056 104 755 2702 Fragments arranged by size: 2702 2056 755 711 104 BsaBI GATnn'nnATC Cuts at: 3096 6322 3096 Size: 3226 3102 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3201 4135 4517 5152 Size: 53 83 186 936 1668 934 382 635 Cuts at: 5152 275 Size: 1451 Fragments arranged by size: 1668 1451 936 934 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4761 5159 5433 5580 5991 4761 Size: 398 274 147 411 5098 Fragments arranged by size: 5098 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6103 Fragments arranged by size: 6103 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2852 3005 3494 1186 Size: 967 699 153 489 4020 Fragments arranged by size: 4020 967 699 489 153 BseYI C'CCAG_C Cuts at: 2330 2621 2810 2822 3209 3290 3618 5531 6027 Size: 291 189 12 387 81 328 1913 496 Cuts at: 6027 2330 Size: 2631 Fragments arranged by size: 2631 1913 496 387 328 291 189 81 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 1163 Size: 573 468 5287 Fragments arranged by size: 5287 573 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3137 3348 3778 4539 5567 1540 Size: 275 186 1136 211 430 761 1028 2301 Fragments arranged by size: 2301 1136 1028 761 430 275 211 186 BsiHKAI G_wGCw'C Cuts at: 2580 3567 4389 4474 5545 2580 Size: 987 822 85 1071 3363 Fragments arranged by size: 3363 1071 987 822 85 BsmI GAATG_Cn' Cuts at: 2469 2959 3839 3932 2469 Size: 490 880 93 4865 Fragments arranged by size: 4865 880 490 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2135 2279 3460 2135 Size: 144 1181 5003 Fragments arranged by size: 5003 1181 144 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2580 2637 2817 3046 3120 3513 Size: 399 475 315 57 180 229 74 393 Cuts at: 3513 3567 3600 3647 4389 4474 5545 1391 Size: 54 33 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 475 399 393 315 229 180 85 74 57 54 47 33 BspEI T'CCGG_A Cuts at: 5991 5991 Size: 6328 BspHI T'CATG_A Cuts at: 2971 4217 5947 2971 Size: 1246 1730 3352 Fragments arranged by size: 3352 1730 1246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3683 844 Size: 2839 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3151 4215 1394 Size: 429 1328 1064 3507 Fragments arranged by size: 3507 1328 1064 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2593 4823 4997 65 Size: 1183 1345 2230 174 1396 Fragments arranged by size: 2230 1396 1345 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4972 5159 1700 Size: 110 3162 187 2869 Fragments arranged by size: 3162 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6328 BssHII G'CGCG_C Cuts at: 1925 2465 1925 Size: 540 5788 BssSI C'ACGA_G Cuts at: 4388 5400 4388 Size: 1012 5316 BstAPI GCAn_nnn'nTGC Cuts at: 794 2503 794 Size: 1709 4619 BstBI TT'CG_AA Cuts at: 5132 5132 Size: 6328 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6328 BstYI r'GATC_y Cuts at: 1972 2030 2108 2190 2613 3091 3658 4025 4410 Size: 58 78 82 423 478 567 367 385 Cuts at: 4410 4427 5879 5965 5977 6323 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 567 478 423 385 367 346 86 82 78 58 17 12 BtgI C'CryG_G Cuts at: 513 1065 2046 3111 3181 3576 513 Size: 552 981 1065 70 395 3265 Fragments arranged by size: 3265 1065 981 552 395 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5589 Fragments arranged by size: 5589 683 56 BtsI GCAGTG_nn' Cuts at: 2560 2567 2605 2812 3844 4638 4658 2560 Size: 7 38 207 1032 794 20 4230 Fragments arranged by size: 4230 1032 794 207 38 20 7 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2575 2610 529 Size: 35 2011 35 4247 Fragments arranged by size: 4247 2011 35 35 DraI TTT'AAA Cuts at: 3632 3632 Size: 6328 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5335 809 Size: 507 4019 1802 Fragments arranged by size: 4019 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 3134 3345 Size: 54 1425 50 275 18 285 1019 211 Cuts at: 3345 3775 4664 6293 8 Size: 430 889 1629 43 Fragments arranged by size: 1629 1425 1019 889 430 285 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3134 3345 3775 1537 Size: 275 1322 211 430 4090 Fragments arranged by size: 4090 1322 430 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 4258 1353 Size: 2905 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3005 3473 4915 5289 5435 387 Size: 1064 138 1416 468 1442 374 146 1280 Fragments arranged by size: 1442 1416 1280 1064 468 374 146 138 EcoICRI GAG'CTC Cuts at: 2578 3565 2578 Size: 987 5341 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2171 2195 2240 2303 2732 2855 Size: 393 524 15 24 45 63 429 123 Cuts at: 2855 2891 3089 3242 3443 3551 3566 4391 4988 Size: 36 198 153 201 108 15 825 597 Cuts at: 4988 5775 6043 1239 Size: 787 268 1524 Fragments arranged by size: 1524 825 787 597 524 429 393 268 201 198 153 123 108 63 45 36 24 15 15 EcoNI CCTnn'n_nnAGG Cuts at: 2117 2117 Size: 6328 EcoO109I rG'GnC_Cy Cuts at: 2200 2866 2892 3379 3552 2200 Size: 666 26 487 173 4976 Fragments arranged by size: 4976 666 487 173 26 EcoRI G'AATT_C Cuts at: 3637 4015 3637 Size: 378 5950 FspI TGC'GCA Cuts at: 2387 2585 4834 2387 Size: 198 2249 3881 Fragments arranged by size: 3881 2249 198 FspAI rTGC'GCAy Cuts at: 2387 2387 Size: 6328 HaeII r_GCGC'y Cuts at: 1678 3217 5475 1678 Size: 1539 2258 2531 Fragments arranged by size: 2531 2258 1539 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3028 3060 4969 5001 5043 Size: 32 135 32 1456 32 1909 32 42 Cuts at: 5043 5075 1373 Size: 32 2626 Fragments arranged by size: 2626 1909 1456 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3316 3672 3918 669 Size: 988 281 1378 356 246 3079 Fragments arranged by size: 3079 1378 988 356 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6328 HpaI GTT'AAC Cuts at: 3918 3918 Size: 6328 KpnI G_GTAC'C Cuts at: 3653 3653 Size: 6328 MfeI C'AATT_G Cuts at: 3927 3927 Size: 6328 MluI A'CGCG_T Cuts at: 6204 6204 Size: 6328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5442 5626 1305 Size: 4137 184 2007 Fragments arranged by size: 4137 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 10 Size: 54 1425 343 285 4221 Fragments arranged by size: 4221 1425 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2424 2514 3113 4286 4645 4804 518 Size: 648 1258 90 599 1173 359 159 2042 Fragments arranged by size: 2042 1258 1173 648 599 359 159 90 MspA1I CmG'CkG Cuts at: 1606 2212 2830 3499 3618 3744 4421 5569 5814 Size: 606 618 669 119 126 677 1148 245 Cuts at: 5814 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 669 618 606 245 126 119 NcoI C'CATG_G Cuts at: 513 1065 3576 513 Size: 552 2511 3265 Fragments arranged by size: 3265 2511 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6328 NotI GC'GGCC_GC Cuts at: 3775 3775 Size: 6328 NspI r_CATG'y Cuts at: 1132 1229 2513 2790 3007 3686 5231 1132 Size: 97 1284 277 217 679 1545 2229 Fragments arranged by size: 2229 1545 1284 679 277 217 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6328 PciI A'CATG_T Cuts at: 5227 5227 Size: 6328 PflMI CCAn_nnn'nTGG Cuts at: 1108 2778 2833 1108 Size: 1670 55 4603 Fragments arranged by size: 4603 1670 55 PfoI T'CCnGG_A Cuts at: 2016 2256 2280 2016 Size: 240 24 6064 Fragments arranged by size: 6064 240 24 PmeI GTTT'AAAC Cuts at: 3632 3632 Size: 6328 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4397 4429 5910 5942 4397 Size: 32 1481 32 4783 Fragments arranged by size: 4783 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2200 2866 2892 3552 2200 Size: 666 26 660 4976 Fragments arranged by size: 4976 666 660 26 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6328 PsiI TTA'TAA Cuts at: 3898 3898 Size: 6328 PstI C_TGCA'G Cuts at: 830 2059 2574 3680 830 Size: 1229 515 1106 3478 Fragments arranged by size: 3478 1229 1106 515 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6328 PvuII CAG'CTG Cuts at: 2212 2830 3618 2212 Size: 618 788 4922 Fragments arranged by size: 4922 788 618 SacI G_AGCT'C Cuts at: 2580 3567 2580 Size: 987 5341 SalI G'TCGA_C Cuts at: 1936 3670 1936 Size: 1734 4594 SanDI GG'GwC_CC Cuts at: 2892 2892 Size: 6328 SbfI CC_TGCA'GG Cuts at: 3680 3680 Size: 6328 ScaI AGT'ACT Cuts at: 4576 6288 4576 Size: 1712 4616 SfcI C'TryA_G Cuts at: 826 1045 2055 2570 3676 4811 5492 5683 826 Size: 219 1010 515 1106 1135 681 191 1471 Fragments arranged by size: 1471 1135 1106 1010 681 515 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6328 SmaI CCC'GGG Cuts at: 1872 3655 1872 Size: 1783 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2609 4432 5333 5595 820 Size: 197 926 666 1823 901 262 1553 Fragments arranged by size: 1823 1553 926 901 666 262 197 SnaBI TAC'GTA Cuts at: 493 4119 493 Size: 3626 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6328 SphI G_CATG'C Cuts at: 1132 2790 3007 3686 1132 Size: 1658 217 679 3774 Fragments arranged by size: 3774 1658 679 217 SspI AAT'ATT Cuts at: 5 52 4252 5 Size: 47 4200 2081 Fragments arranged by size: 4200 2081 47 StuI AGG'CCT Cuts at: 1517 2502 2721 1517 Size: 985 219 5124 Fragments arranged by size: 5124 985 219 StyI C'CwwG_G Cuts at: 513 1065 2118 3576 3767 513 Size: 552 1053 1458 191 3074 Fragments arranged by size: 3074 1458 1053 552 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2414 2504 3595 3610 4355 4372 4525 Size: 1255 834 90 1091 15 745 17 153 Cuts at: 4525 4710 5155 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1091 834 745 445 185 153 90 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4574 6286 96 Size: 274 80 33 51 1443 2597 1712 138 Fragments arranged by size: 2597 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2258 2290 6288 6320 2258 Size: 32 3998 32 2266 Fragments arranged by size: 3998 2266 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6328 XbaI T'CTAG_A Cuts at: 3664 3664 Size: 6328 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2338 2830 3277 2338 Size: 492 447 5389 Fragments arranged by size: 5389 492 447 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6328 XmaI C'CCGG_G Cuts at: 1870 3653 1870 Size: 1783 4545 XmnI GAAnn'nnTTC Cuts at: 4457 4457 Size: 6328 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3202 4136 276 Size: 53 83 186 936 1668 934 2468 Fragments arranged by size: 2468 1668 936 934 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI