(Circular) (Six-base) MAPSORT of: pFN21AB7022.seq Check: 1445 from: 1 to: 7621 pFN21AB7022 7621 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4518 5431 278 Size: 53 83 186 936 2982 913 2468 Fragments arranged by size: 2982 2468 936 913 186 83 53 AccI GT'mk_AC Cuts at: 1937 4095 4497 4964 1937 Size: 2158 402 467 4594 Fragments arranged by size: 4594 2158 467 402 Acc65I G'GTAC_C Cuts at: 3273 4942 3273 Size: 1669 5952 AclI AA'CG_TT Cuts at: 1563 5748 6121 1563 Size: 4185 373 3063 Fragments arranged by size: 4185 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3137 3857 3878 3926 4718 5684 7068 7336 Size: 1505 720 21 48 792 966 1384 268 Cuts at: 7336 1632 Size: 1917 Fragments arranged by size: 1917 1505 1384 966 792 720 268 48 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7424 AflIII A'CryG_T Cuts at: 1203 6520 7497 1203 Size: 5317 977 1327 Fragments arranged by size: 5317 1327 977 AgeI A'CCGG_T Cuts at: 6452 6452 Size: 7621 AhdI GACnn_n'nnGTC Cuts at: 4368 4519 6350 4368 Size: 151 1831 5639 Fragments arranged by size: 5639 1831 151 AleI CACnn'nnGTG Cuts at: 1166 3554 4172 1166 Size: 2388 618 4615 Fragments arranged by size: 4615 2388 618 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7621 AlwNI CAG_nnn'CTG Cuts at: 3415 5364 6936 3415 Size: 1949 1572 4100 Fragments arranged by size: 4100 1949 1572 ApaI G_GGCC'C Cuts at: 2012 2610 3466 2012 Size: 598 856 6167 Fragments arranged by size: 6167 856 598 ApaLI G'TGCA_C Cuts at: 5678 6834 5678 Size: 1156 6465 ApoI r'AATT_y Cuts at: 1493 4930 5156 5308 7417 1493 Size: 3437 226 152 2109 1697 Fragments arranged by size: 3437 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6175 160 Size: 872 5143 1606 Fragments arranged by size: 5143 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7621 AvaI C'yCGr_G Cuts at: 1870 1943 4082 4115 4946 1870 Size: 73 2139 33 831 4545 Fragments arranged by size: 4545 2139 831 73 33 BamHI G'GATC_C Cuts at: 4951 5318 4951 Size: 367 7254 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2626 3248 3273 4942 6397 Size: 325 217 627 839 622 25 1669 1455 Cuts at: 6397 618 Size: 1842 Fragments arranged by size: 1842 1669 1455 839 627 622 325 217 25 BanII G_rGCy'C Cuts at: 1391 2012 2079 2610 2918 2961 3051 3178 3192 Size: 621 67 531 308 43 90 127 14 Cuts at: 3192 3390 3466 3520 4070 4260 4695 4844 4940 Size: 198 76 54 550 190 435 149 96 Cuts at: 4940 1391 Size: 4072 Fragments arranged by size: 4072 621 550 531 435 308 198 190 149 127 96 90 76 67 54 43 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2701 928 Size: 971 802 5848 Fragments arranged by size: 5848 971 802 BbvCI CC'TCA_GC Cuts at: 2210 4041 2210 Size: 1831 5790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2400 2434 5812 5846 1903 Size: 34 463 34 3378 34 3678 Fragments arranged by size: 3678 3378 463 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4613 5513 6723 4613 Size: 900 1210 5511 Fragments arranged by size: 5511 1210 900 BclI T'GATC_A Cuts at: 1548 1548 Size: 7621 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3924 4682 6232 Size: 107 122 71 631 605 2252 758 1550 Cuts at: 6232 136 Size: 1525 Fragments arranged by size: 2252 1550 1525 758 631 605 122 107 71 BglII A'GATC_T Cuts at: 4545 4719 7616 4545 Size: 174 2897 4550 Fragments arranged by size: 4550 2897 174 BlpI GC'TnA_GC Cuts at: 2677 5038 2677 Size: 2361 5260 Bme1580I G_kGCm'C Cuts at: 1790 2012 2542 2610 3466 5682 6838 1790 Size: 222 530 68 856 2216 1156 2573 Fragments arranged by size: 2573 2216 1156 856 530 222 68 BmgBI CAC'GTC Cuts at: 1318 4339 1318 Size: 3021 4600 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2060 2073 5352 Size: 416 53 83 399 24 643 13 3279 Cuts at: 5352 6310 442 Size: 958 1753 Fragments arranged by size: 3279 1753 958 643 416 399 83 53 24 13 BmtI G_CTAG'C Cuts at: 1056 2339 1056 Size: 1283 6338 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4567 4858 4567 Size: 291 7330 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2182 2286 3190 3795 4135 4264 4274 4850 Size: 943 104 904 605 340 129 10 576 Cuts at: 4850 6281 1239 Size: 1431 2579 Fragments arranged by size: 2579 1431 943 904 605 576 340 129 104 10 Bpu10I CC'TnA_GC Cuts at: 2088 2210 3045 3377 4041 4064 2088 Size: 122 835 332 664 23 5645 Fragments arranged by size: 5645 835 664 332 122 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2932 5746 6611 6909 2932 Size: 2814 865 298 3644 Fragments arranged by size: 3644 2814 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2262 3422 6284 882 Size: 617 763 1160 2862 2219 Fragments arranged by size: 2862 2219 1160 763 617 BsaAI yAC'GTr Cuts at: 493 1204 5412 493 Size: 711 4208 2702 Fragments arranged by size: 4208 2702 711 BsaBI GATnn'nnATC Cuts at: 2567 7615 2567 Size: 5048 2573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2415 4092 4515 5428 Size: 53 83 186 936 882 1677 423 913 Cuts at: 5428 5810 6445 275 Size: 382 635 1451 Fragments arranged by size: 1677 1451 936 913 882 635 423 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6054 6452 6726 6873 7284 6054 Size: 398 274 147 411 6391 Fragments arranged by size: 6391 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2100 2130 2855 2885 3963 Size: 30 165 30 697 30 725 30 1078 Cuts at: 3963 3993 1178 Size: 30 4806 Fragments arranged by size: 4806 1078 725 697 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4526 4847 4850 1186 Size: 3340 321 3 3957 Fragments arranged by size: 3957 3340 321 3 BseYI C'CCAG_C Cuts at: 2611 3279 4051 4058 4632 4680 4884 6824 7320 Size: 668 772 7 574 48 204 1940 496 Cuts at: 7320 2611 Size: 2912 Fragments arranged by size: 2912 1940 772 668 574 496 204 48 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2239 2609 2873 3325 3428 3478 1163 Size: 573 503 370 264 452 103 50 5306 Fragments arranged by size: 5306 573 503 452 370 264 103 50 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5071 5832 6860 1540 Size: 275 186 3070 761 1028 2301 Fragments arranged by size: 3070 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2678 2918 3178 3192 3441 4260 4844 4854 5682 Size: 240 260 14 249 819 584 10 828 Cuts at: 5682 5767 6838 2678 Size: 85 1071 3461 Fragments arranged by size: 3461 1071 828 819 584 260 249 240 85 14 10 BsmI GAATG_Cn' Cuts at: 5132 5225 5132 Size: 93 7528 BsmBI CGTCTCn'nnnn_ Cuts at: 2422 2422 Size: 7621 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2079 2542 2610 2678 2918 2961 Size: 399 222 67 463 68 68 240 43 Cuts at: 2961 3051 3178 3192 3390 3441 3466 3520 4070 Size: 90 127 14 198 51 25 54 550 Cuts at: 4070 4260 4695 4844 4854 4940 5682 5767 6838 Size: 190 435 149 10 86 742 85 1071 Cuts at: 6838 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 550 463 435 399 240 222 198 190 149 127 90 86 85 68 68 67 54 51 43 25 14 10 BspEI T'CCGG_A Cuts at: 7284 7284 Size: 7621 BspHI T'CATG_A Cuts at: 3912 4413 5510 7240 3912 Size: 501 1097 1730 4293 Fragments arranged by size: 4293 1730 1097 501 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3337 3756 4023 4275 4868 4976 844 Size: 2493 419 267 252 593 108 3489 Fragments arranged by size: 3489 2493 593 419 267 252 108 BsrBI CCG'CTC Cuts at: 1394 1823 4765 5508 1394 Size: 429 2942 743 3507 Fragments arranged by size: 3507 2942 743 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2143 4450 6116 6290 65 Size: 1183 895 2307 1666 174 1396 Fragments arranged by size: 2307 1666 1396 1183 895 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2439 2822 6265 6452 1700 Size: 110 629 383 3443 187 2869 Fragments arranged by size: 3443 2869 629 383 187 110 BsrGI T'GTAC_A Cuts at: 96 3615 96 Size: 3519 4102 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7621 BssSI C'ACGA_G Cuts at: 3932 5681 6693 3932 Size: 1749 1012 4860 Fragments arranged by size: 4860 1749 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3415 794 Size: 2621 5000 BstBI TT'CG_AA Cuts at: 6425 6425 Size: 7621 BstEII G'GTnAC_C Cuts at: 1184 2116 2941 1184 Size: 932 825 5864 Fragments arranged by size: 5864 932 825 BstXI CCAn_nnnn'nTGG Cuts at: 3168 3168 Size: 7621 BstYI r'GATC_y Cuts at: 1972 4545 4719 4951 5318 5703 5720 7172 7258 Size: 2573 174 232 367 385 17 1452 86 Cuts at: 7258 7270 7616 1972 Size: 12 346 1977 Fragments arranged by size: 2573 1977 1452 385 367 346 232 174 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3369 3369 Size: 7621 BtgI C'CryG_G Cuts at: 513 1065 2004 2946 3801 3864 3967 513 Size: 552 939 942 855 63 103 4167 Fragments arranged by size: 4167 942 939 855 552 103 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2140 487 Size: 683 56 914 5968 Fragments arranged by size: 5968 914 683 56 BtsI GCAGTG_nn' Cuts at: 2542 3308 3313 4828 5137 5931 5951 2542 Size: 766 5 1515 309 794 20 4212 Fragments arranged by size: 4212 1515 794 766 309 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7621 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3790 3825 529 Size: 35 3226 35 4325 Fragments arranged by size: 4325 3226 35 35 DraI TTT'AAA Cuts at: 4925 4925 Size: 7621 DraIII CAC_nnn'GTG Cuts at: 3400 3400 Size: 7621 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6628 809 Size: 507 5312 1802 Fragments arranged by size: 5312 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2437 2841 Size: 54 1425 50 275 18 207 400 404 Cuts at: 2841 3133 3150 3310 4072 4377 5068 5957 7586 Size: 292 17 160 762 305 691 889 1629 Cuts at: 7586 8 Size: 43 Fragments arranged by size: 1629 1425 889 762 691 404 400 305 292 275 207 160 54 50 43 18 17 EagI C'GGCC_G Cuts at: 1537 1812 5068 1537 Size: 275 3256 4090 Fragments arranged by size: 4090 3256 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3551 5551 1353 Size: 2198 2000 3423 Fragments arranged by size: 3423 2198 2000 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6208 6582 6728 387 Size: 1064 138 4619 374 146 1280 Fragments arranged by size: 4619 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2916 3176 3190 4258 4842 2916 Size: 260 14 1068 584 5695 Fragments arranged by size: 5695 1068 584 260 14 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2182 2286 3137 3190 3795 3857 3878 Size: 393 550 104 851 53 605 62 21 Cuts at: 3878 3926 4135 4264 4274 4718 4850 5684 6281 Size: 48 209 129 10 444 132 834 597 Cuts at: 6281 7068 7336 1239 Size: 787 268 1524 Fragments arranged by size: 1524 851 834 787 605 597 550 444 393 268 209 132 129 104 62 53 48 21 10 EcoNI CCTnn'n_nnAGG Cuts at: 2933 3380 4232 2933 Size: 447 852 6322 Fragments arranged by size: 6322 852 447 EcoO109I rG'GnC_Cy Cuts at: 2244 2606 2607 3023 3373 3462 3889 3974 4055 Size: 362 1 416 350 89 427 85 81 Cuts at: 4055 4078 4264 2244 Size: 23 186 5601 Fragments arranged by size: 5601 427 416 362 350 186 89 85 81 23 1 EcoRI G'AATT_C Cuts at: 4930 5308 4930 Size: 378 7243 EcoRV GAT'ATC Cuts at: 4879 4879 Size: 7621 FseI GG_CCGG'CC Cuts at: 2443 2443 Size: 7621 FspI TGC'GCA Cuts at: 6127 6127 Size: 7621 HaeII r_GCGC'y Cuts at: 1678 2289 6768 1678 Size: 611 4479 2531 Fragments arranged by size: 4479 2531 611 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4199 4231 4354 4386 4505 Size: 32 135 32 2627 32 123 32 119 Cuts at: 4505 4537 6262 6294 6336 6368 1373 Size: 32 1725 32 42 32 2626 Fragments arranged by size: 2627 2626 1725 135 123 119 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2698 3946 4096 4362 4965 5211 Size: 988 281 760 1248 150 266 603 246 Cuts at: 5211 669 Size: 3079 Fragments arranged by size: 3079 1248 988 760 603 281 266 246 150 HindIII A'AGCT_T Cuts at: 748 3053 748 Size: 2305 5316 HpaI GTT'AAC Cuts at: 2698 5211 2698 Size: 2513 5108 KpnI G_GTAC'C Cuts at: 3277 4946 3277 Size: 1669 5952 MfeI C'AATT_G Cuts at: 5220 5220 Size: 7621 MluI A'CGCG_T Cuts at: 7497 7497 Size: 7621 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2740 2839 4449 6735 6919 1305 Size: 1435 99 1610 2286 184 2007 Fragments arranged by size: 2286 2007 1610 1435 184 99 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 2843 3152 3312 4379 Size: 54 1425 343 207 804 309 160 1067 Cuts at: 4379 10 Size: 3252 Fragments arranged by size: 3252 1425 1067 804 343 309 207 160 54 MslI CAynn'nnrTG Cuts at: 518 1166 2301 2873 3554 3876 4172 4583 5579 Size: 648 1135 572 681 322 296 411 996 Cuts at: 5579 5938 6097 518 Size: 359 159 2042 Fragments arranged by size: 2042 1135 996 681 648 572 411 359 322 296 159 MspA1I CmG'CkG Cuts at: 1606 3651 3709 4809 5037 5714 6862 7107 1606 Size: 2045 58 1100 228 677 1148 245 2120 Fragments arranged by size: 2120 2045 1148 1100 677 245 228 58 NaeI GCC'GGC Cuts at: 2441 2824 2441 Size: 383 7238 NcoI C'CATG_G Cuts at: 513 1065 2004 3967 513 Size: 552 939 1963 4167 Fragments arranged by size: 4167 1963 939 552 NdeI CA'TA_TG Cuts at: 387 3224 387 Size: 2837 4784 NgoMIV G'CCGG_C Cuts at: 2439 2822 2439 Size: 383 7238 NheI G'CTAG_C Cuts at: 1052 2335 1052 Size: 1283 6338 NotI GC'GGCC_GC Cuts at: 5068 5068 Size: 7621 NspI r_CATG'y Cuts at: 1132 1229 2822 3574 4979 6524 1132 Size: 97 1593 752 1405 1545 2229 Fragments arranged by size: 2229 1593 1545 1405 752 97 PasI CC'CwG_GG Cuts at: 1348 2275 2603 4294 1348 Size: 927 328 1691 4675 Fragments arranged by size: 4675 1691 927 328 PciI A'CATG_T Cuts at: 6520 6520 Size: 7621 PflMI CCAn_nnn'nTGG Cuts at: 1108 2046 4162 4300 1108 Size: 938 2116 138 4429 Fragments arranged by size: 4429 2116 938 138 PfoI T'CCnGG_A Cuts at: 2934 4260 2934 Size: 1326 6295 PmeI GTTT'AAAC Cuts at: 4925 4925 Size: 7621 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2900 2932 5690 5722 7203 7235 2900 Size: 32 2758 32 1481 32 3286 Fragments arranged by size: 3286 2758 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2244 3023 3373 4055 4078 4264 2244 Size: 779 350 682 23 186 5601 Fragments arranged by size: 5601 779 682 350 186 23 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7621 PsiI TTA'TAA Cuts at: 5191 5191 Size: 7621 PspOMI G'GGCC_C Cuts at: 2008 2606 3462 2008 Size: 598 856 6167 Fragments arranged by size: 6167 856 598 PstI C_TGCA'G Cuts at: 830 2635 3303 3348 3514 3753 4899 4973 830 Size: 1805 668 45 166 239 1146 74 3478 Fragments arranged by size: 3478 1805 1146 668 239 166 74 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7621 PvuII CAG'CTG Cuts at: 3709 3709 Size: 7621 SacI G_AGCT'C Cuts at: 2918 3178 3192 4260 4844 2918 Size: 260 14 1068 584 5695 Fragments arranged by size: 5695 1068 584 260 14 SalI G'TCGA_C Cuts at: 1936 4094 4963 1936 Size: 2158 869 4594 Fragments arranged by size: 4594 2158 869 SanDI GG'GwC_CC Cuts at: 3023 4055 3023 Size: 1032 6589 SbfI CC_TGCA'GG Cuts at: 4973 4973 Size: 7621 ScaI AGT'ACT Cuts at: 3998 5869 7581 3998 Size: 1871 1712 4038 Fragments arranged by size: 4038 1871 1712 SexAI A'CCwGG_T Cuts at: 3642 3642 Size: 7621 SfcI C'TryA_G Cuts at: 826 1045 2631 3299 3344 3510 3749 4895 4969 Size: 219 1586 668 45 166 239 1146 74 Cuts at: 4969 6104 6785 6976 826 Size: 1135 681 191 1471 Fragments arranged by size: 1586 1471 1146 1135 681 668 239 219 191 166 74 45 SfiI GGCCn_nnn'nGGCC Cuts at: 4682 4682 Size: 7621 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7621 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7621 SmaI CCC'GGG Cuts at: 1872 4948 1872 Size: 3076 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2911 4115 5725 6626 6888 820 Size: 197 926 968 1204 1610 901 262 1553 Fragments arranged by size: 1610 1553 1204 968 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5412 493 Size: 4919 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7621 SphI G_CATG'C Cuts at: 1132 4979 1132 Size: 3847 3774 SspI AAT'ATT Cuts at: 5 52 5545 5 Size: 47 5493 2081 Fragments arranged by size: 5493 2081 47 StuI AGG'CCT Cuts at: 1517 2984 2994 4186 4676 1517 Size: 1467 10 1192 490 4462 Fragments arranged by size: 4462 1467 1192 490 10 StyI C'CwwG_G Cuts at: 513 1065 2004 3072 3967 4686 5060 513 Size: 552 939 1068 895 719 374 3074 Fragments arranged by size: 3074 1068 939 895 719 552 374 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2537 4712 5648 5665 5818 6003 6448 Size: 1255 957 2175 936 17 153 185 445 Cuts at: 6448 325 Size: 1498 Fragments arranged by size: 2175 1498 1255 957 936 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 3615 3979 Size: 274 80 33 51 1443 207 1431 364 Cuts at: 3979 3996 5867 7579 96 Size: 17 1871 1712 138 Fragments arranged by size: 1871 1712 1443 1431 364 274 207 138 80 51 33 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2052 2084 2100 2132 2855 2887 7581 7613 2052 Size: 32 16 32 723 32 4694 32 2060 Fragments arranged by size: 4694 2060 723 32 32 32 32 16 Tth111I GACn'n_nGTC Cuts at: 1316 4493 1316 Size: 3177 4444 XbaI T'CTAG_A Cuts at: 4957 4957 Size: 7621 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2172 4159 4372 2172 Size: 1987 213 5421 Fragments arranged by size: 5421 1987 213 XhoI C'TCGA_G Cuts at: 1943 4115 1943 Size: 2172 5449 XmaI C'CCGG_G Cuts at: 1870 4946 1870 Size: 3076 4545 XmnI GAAnn'nnTTC Cuts at: 5750 5750 Size: 7621 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4516 5429 276 Size: 53 83 186 936 2982 913 2468 Fragments arranged by size: 2982 2468 936 913 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SfoI SrfI SwaI