(Circular) (Six-base) MAPSORT of: pFN21AB9405.seq Check: 2533 from: 1 to: 6217 pFN21AB9405 6217 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2673 4280 5664 5932 1632 Size: 1041 1607 1384 268 1917 Fragments arranged by size: 1917 1607 1384 1041 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 3463 4946 3463 Size: 1483 4734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 2345 2935 3960 5532 2345 Size: 590 1025 1572 3030 Fragments arranged by size: 3030 1572 1025 590 ApaI G_GGCC'C Cuts at: 2090 2090 Size: 6217 ApaLI G'TGCA_C Cuts at: 2168 4274 5430 2168 Size: 2106 1156 2955 Fragments arranged by size: 2955 2106 1156 ApoI r'AATT_y Cuts at: 1493 2739 3019 3526 3752 3904 6013 1493 Size: 1246 280 507 226 152 2109 1697 Fragments arranged by size: 2109 1697 1246 507 280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2012 2102 3107 3183 3542 1870 Size: 73 69 90 1005 76 359 4545 Fragments arranged by size: 4545 1005 359 90 76 73 69 BamHI G'GATC_C Cuts at: 2008 3547 3914 2008 Size: 1539 367 4311 Fragments arranged by size: 4311 1539 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2113 2134 2143 3170 3538 Size: 325 217 627 326 21 9 1027 368 Cuts at: 3538 4993 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1027 627 368 326 325 217 21 9 BanII G_rGCy'C Cuts at: 1391 2090 2307 2356 2380 2873 3536 1391 Size: 699 217 49 24 493 663 4072 Fragments arranged by size: 4072 699 663 493 217 49 24 BbeI G_GCGC'C Cuts at: 2138 2138 Size: 6217 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2484 3093 928 Size: 971 585 609 4052 Fragments arranged by size: 4052 971 609 585 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3268 4109 5319 3268 Size: 841 1210 4166 Fragments arranged by size: 4166 1210 841 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2263 4828 136 Size: 107 122 71 631 605 591 2565 1525 Fragments arranged by size: 2565 1525 631 605 591 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 2475 3634 2475 Size: 1159 5058 Bme1580I G_kGCm'C Cuts at: 1790 2090 2172 2247 2259 4278 5434 1790 Size: 300 82 75 12 2019 1156 2573 Fragments arranged by size: 2573 2019 1156 300 82 75 12 BmgBI CAC'GTC Cuts at: 1318 2173 1318 Size: 855 5362 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3948 4906 442 Size: 416 53 83 399 24 2531 958 1753 Fragments arranged by size: 2531 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2373 2460 2820 3089 4877 1239 Size: 980 154 87 360 269 1788 2579 Fragments arranged by size: 2579 1788 980 360 269 154 87 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2183 4880 882 Size: 617 684 2697 2219 Fragments arranged by size: 2697 2219 684 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2135 2883 4024 4406 Size: 53 83 186 936 602 748 1141 382 Cuts at: 4406 5041 275 Size: 635 1451 Fragments arranged by size: 1451 1141 936 748 635 602 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2096 2268 4650 5048 5322 5469 5880 2096 Size: 172 2382 398 274 147 411 2433 Fragments arranged by size: 2433 2382 411 398 274 172 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5992 Fragments arranged by size: 5992 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3422 1186 Size: 2236 3981 BseYI C'CCAG_C Cuts at: 2455 3008 5420 5916 2455 Size: 553 2412 496 2756 Fragments arranged by size: 2756 2412 553 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 1163 Size: 573 948 4696 Fragments arranged by size: 4696 948 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2380 2836 4278 4363 5434 2172 Size: 208 456 1442 85 1071 2955 Fragments arranged by size: 2955 1442 1071 456 208 85 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2172 2247 2259 2307 2356 2380 Size: 399 300 82 75 12 48 49 24 Cuts at: 2380 2836 2873 3536 4278 4363 5434 1391 Size: 456 37 663 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 663 456 399 300 85 82 75 49 48 37 24 12 BspEI T'CCGG_A Cuts at: 2096 2268 5880 2096 Size: 172 3612 2433 Fragments arranged by size: 3612 2433 172 BspHI T'CATG_A Cuts at: 2220 4106 5836 2220 Size: 1886 1730 2601 Fragments arranged by size: 2601 1886 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2519 3572 844 Size: 1675 1053 3489 Fragments arranged by size: 3489 1675 1053 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2997 4712 4886 65 Size: 1183 1749 1715 174 1396 Fragments arranged by size: 1749 1715 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 2110 4861 5048 1700 Size: 110 225 75 2751 187 2869 Fragments arranged by size: 2869 2751 225 187 110 75 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 2345 2920 2935 2977 2992 794 Size: 1551 575 15 42 15 4019 Fragments arranged by size: 4019 1551 575 42 15 15 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstXI CCAn_nnnn'nTGG Cuts at: 2532 2532 Size: 6217 BstYI r'GATC_y Cuts at: 1972 2008 3151 3547 3914 4299 4316 5768 5854 Size: 36 1143 396 367 385 17 1452 86 Cuts at: 5854 5866 6212 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1143 396 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2118 2176 513 Size: 552 939 114 58 4554 Fragments arranged by size: 4554 939 552 114 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2343 3733 4527 4547 2343 Size: 1390 794 20 4013 Fragments arranged by size: 4013 1390 794 20 ClaI AT'CG_AT Cuts at: 1554 2583 1554 Size: 1029 5188 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2235 3664 4553 Size: 54 1425 50 275 18 405 1429 889 Cuts at: 4553 6182 8 Size: 1629 43 Fragments arranged by size: 1629 1429 1425 889 405 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2213 2703 4147 1353 Size: 860 490 1444 3423 Fragments arranged by size: 3423 1444 860 490 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4804 5178 5324 387 Size: 1064 138 3215 374 146 1280 Fragments arranged by size: 3215 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2378 2378 Size: 6217 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2373 2460 2673 2820 3089 4280 Size: 393 587 154 87 213 147 269 1191 Cuts at: 4280 4877 5664 5932 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1191 787 597 587 393 269 268 213 154 147 87 EcoO109I rG'GnC_Cy Cuts at: 2086 2605 2693 2086 Size: 519 88 5610 Fragments arranged by size: 5610 519 88 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2996 2996 Size: 6217 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2138 2207 5364 1678 Size: 460 69 3157 2531 Fragments arranged by size: 3157 2531 460 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2294 2326 2809 2841 3076 Size: 32 135 32 722 32 483 32 235 Cuts at: 3076 3108 3406 3438 3449 3481 4858 4890 4932 Size: 32 298 32 11 32 1377 32 42 Cuts at: 4932 4964 1373 Size: 32 2626 Fragments arranged by size: 2626 1377 722 483 298 235 135 42 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 3432 3499 748 Size: 2684 67 3466 Fragments arranged by size: 3466 2684 67 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KasI G'GCGC_C Cuts at: 2134 2134 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2928 5331 5515 1305 Size: 1623 2403 184 2007 Fragments arranged by size: 2403 2007 1623 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4395 Fragments arranged by size: 4395 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4175 4534 4693 518 Size: 648 3009 359 159 2042 Fragments arranged by size: 3009 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2120 2178 3633 4310 5458 5703 1606 Size: 514 58 1455 677 1148 245 2120 Fragments arranged by size: 2120 1455 1148 677 514 245 58 NarI GG'CG_CC Cuts at: 2135 2135 Size: 6217 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4726 Fragments arranged by size: 4726 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2372 3575 5120 1132 Size: 97 1143 1203 1545 2229 Fragments arranged by size: 2229 1545 1203 1143 97 PasI CC'CwG_GG Cuts at: 1348 2044 1348 Size: 696 5521 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2775 1108 Size: 1667 4550 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2605 2693 2605 Size: 88 6129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 2086 2086 Size: 6217 PstI C_TGCA'G Cuts at: 830 2350 2403 2931 2940 3429 3569 830 Size: 1520 53 528 9 489 140 3478 Fragments arranged by size: 3478 1520 528 489 140 53 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 SacI G_AGCT'C Cuts at: 2380 2380 Size: 6217 SacII CC_GC'GG Cuts at: 2121 2179 2121 Size: 58 6159 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SapI GCTCTTCn'nnn_ Cuts at: 2213 2213 Size: 6217 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 2332 4465 6177 2332 Size: 2133 1712 2372 Fragments arranged by size: 2372 2133 1712 SfcI C'TryA_G Cuts at: 826 1045 2346 2399 2927 2936 3223 3425 3565 Size: 219 1301 53 528 9 287 202 140 Cuts at: 3565 4700 5381 5572 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1301 1135 681 528 287 219 202 191 140 53 9 SfoI GGC'GCC Cuts at: 2136 2136 Size: 6217 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 2014 2104 3109 3544 1872 Size: 142 90 1005 435 4545 Fragments arranged by size: 4545 1005 435 142 90 SmlI C'TyrA_G Cuts at: 820 1017 1943 3183 4321 5222 5484 820 Size: 197 926 1240 1138 901 262 1553 Fragments arranged by size: 1553 1240 1138 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6217 StyI C'CwwG_G Cuts at: 513 1065 2004 2050 2786 3367 3656 513 Size: 552 939 46 736 581 289 3074 Fragments arranged by size: 3074 939 736 581 552 289 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2207 2482 2879 4244 4261 4414 4599 Size: 1255 627 275 397 1365 17 153 185 Cuts at: 4599 5044 325 Size: 445 1498 Fragments arranged by size: 1498 1365 1255 627 445 397 275 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2330 3194 4463 Size: 274 80 33 51 1443 353 864 1269 Cuts at: 4463 6175 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1269 864 353 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6177 6209 6177 Size: 32 6185 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XhoI C'TCGA_G Cuts at: 1943 3183 1943 Size: 1240 4977 XmaI C'CCGG_G Cuts at: 1870 2012 2102 3107 3542 1870 Size: 142 90 1005 435 4545 Fragments arranged by size: 4545 1005 435 142 90 XmnI GAAnn'nnTTC Cuts at: 2979 3323 3345 3501 4346 2979 Size: 344 22 156 845 4850 Fragments arranged by size: 4850 845 344 156 22 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SrfI SwaI XcmI