(Circular) (Six-base) MAPSORT of: pFN21AB0498.seq Check: 8509 from: 1 to: 5959 pFN21AB0498 5959 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2656 2656 Size: 5959 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2369 2477 4022 5406 5674 1632 Size: 737 108 1545 1384 268 1917 Fragments arranged by size: 1917 1545 1384 737 268 108 AfeI AGC'GCT Cuts at: 3181 3181 Size: 5959 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 2143 3702 5274 2143 Size: 1559 1572 2828 Fragments arranged by size: 2828 1572 1559 ApaI G_GGCC'C Cuts at: 2150 2150 Size: 5959 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 3268 3494 3646 5755 1493 Size: 1775 226 152 2109 1697 Fragments arranged by size: 2109 1775 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2220 2966 4513 160 Size: 872 1188 746 1547 1606 Fragments arranged by size: 1606 1547 1188 872 746 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3284 1870 Size: 73 1341 4545 Fragments arranged by size: 4545 1341 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3280 4735 618 Size: 325 217 627 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2150 2467 2741 3278 1391 Size: 759 317 274 537 4072 Fragments arranged by size: 4072 759 537 317 274 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2988 928 Size: 971 1089 3899 Fragments arranged by size: 3899 1089 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2816 2850 4150 4184 1903 Size: 34 879 34 1300 34 3678 Fragments arranged by size: 3678 1300 879 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2587 5954 2587 Size: 3367 2592 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2150 2260 4020 5176 1790 Size: 360 110 1760 1156 2573 Fragments arranged by size: 2573 1760 1156 360 110 BmgBI CAC'GTC Cuts at: 1318 2039 1318 Size: 721 5238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3214 3690 4648 Size: 416 53 83 399 24 1797 476 958 Cuts at: 4648 442 Size: 1753 Fragments arranged by size: 1797 1753 958 476 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2951 4619 1239 Size: 1712 1668 2579 Fragments arranged by size: 2579 1712 1668 Bpu10I CC'TnA_GC Cuts at: 2735 2735 Size: 5959 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2384 2943 4084 4949 5247 2384 Size: 559 1141 865 298 3096 Fragments arranged by size: 3096 1141 865 559 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4622 882 Size: 617 3123 2219 Fragments arranged by size: 3123 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2317 3750 493 Size: 711 1113 1433 2702 Fragments arranged by size: 2702 1433 1113 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3766 4148 4783 275 Size: 53 83 186 936 2233 382 635 1451 Fragments arranged by size: 2233 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2708 2738 1178 Size: 30 165 30 1305 30 4399 Fragments arranged by size: 4399 1305 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2621 1186 Size: 1435 4524 BseYI C'CCAG_C Cuts at: 2143 2253 2687 3055 5162 5658 2143 Size: 110 434 368 2107 496 2444 Fragments arranged by size: 2444 2107 496 434 368 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5386 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2633 3409 4170 5198 1540 Size: 275 186 632 776 761 1028 2301 Fragments arranged by size: 2301 1028 776 761 632 275 186 BsiHKAI G_wGCw'C Cuts at: 2741 4020 4105 5176 2741 Size: 1279 85 1071 3524 Fragments arranged by size: 3524 1279 1071 85 BsmI GAATG_Cn' Cuts at: 2488 3470 3563 2488 Size: 982 93 4884 Fragments arranged by size: 4884 982 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2150 2260 2467 2741 3278 4020 4105 Size: 399 360 110 207 274 537 742 85 Cuts at: 4105 5176 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 537 399 360 274 207 110 85 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 2365 3848 5578 2365 Size: 1483 1730 2746 Fragments arranged by size: 2746 1730 1483 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2656 2821 3314 844 Size: 1812 165 493 3489 Fragments arranged by size: 3489 1812 493 165 BsrBI CCG'CTC Cuts at: 1394 1823 2815 3846 1394 Size: 429 992 1031 3507 Fragments arranged by size: 3507 1031 992 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2704 4603 4790 1700 Size: 110 894 1899 187 2869 Fragments arranged by size: 2869 1899 894 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 2668 3097 1184 Size: 1484 429 4046 Fragments arranged by size: 4046 1484 429 BstYI r'GATC_y Cuts at: 1972 2587 3155 3289 3656 4041 4058 5510 5596 Size: 615 568 134 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 615 568 385 367 346 134 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2132 2468 2132 Size: 336 5623 BtgI C'CryG_G Cuts at: 513 1065 2748 513 Size: 552 1683 3724 Fragments arranged by size: 3724 1683 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2529 2564 529 Size: 35 1965 35 3924 Fragments arranged by size: 3924 1965 35 35 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 3406 4295 Size: 54 1425 50 275 18 396 1180 889 Cuts at: 4295 5924 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1180 889 396 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 2573 2855 3215 3889 1353 Size: 747 473 282 360 674 3423 Fragments arranged by size: 3423 747 674 473 360 282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2193 2723 2734 4546 4920 5066 Size: 1064 138 604 530 11 1812 374 146 Cuts at: 5066 387 Size: 1280 Fragments arranged by size: 1812 1280 1064 604 530 374 146 138 11 EcoICRI GAG'CTC Cuts at: 2739 2739 Size: 5959 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2477 2951 4022 4619 5406 5674 Size: 393 737 108 474 1071 597 787 268 Cuts at: 5674 1239 Size: 1524 Fragments arranged by size: 1524 1071 787 737 597 474 393 268 108 EcoO109I rG'GnC_Cy Cuts at: 2128 2395 3163 2128 Size: 267 768 4924 Fragments arranged by size: 4924 768 267 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2045 2960 2045 Size: 915 5044 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2876 3183 5106 1678 Size: 1198 307 1923 2531 Fragments arranged by size: 2531 1923 1198 307 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2572 2604 2708 2740 4600 Size: 32 135 32 1000 32 104 32 1860 Cuts at: 4600 4632 4674 4706 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1860 1000 135 104 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2293 3303 3549 669 Size: 988 281 355 1010 246 3079 Fragments arranged by size: 3079 1010 988 355 281 246 HindIII A'AGCT_T Cuts at: 748 2534 748 Size: 1786 4173 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2228 10 Size: 54 1425 343 396 3741 Fragments arranged by size: 3741 1425 396 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2370 3917 4276 4435 518 Size: 648 1204 1547 359 159 2042 Fragments arranged by size: 2042 1547 1204 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2347 2625 2659 2773 2794 2855 3375 4052 Size: 741 278 34 114 21 61 520 677 Cuts at: 4052 5200 5445 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 741 677 520 278 245 114 61 34 21 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 2644 1348 Size: 1296 4663 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2808 2928 2808 Size: 120 5839 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3163 3163 Size: 5959 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2146 2146 Size: 5959 PstI C_TGCA'G Cuts at: 830 2664 2723 2799 3311 830 Size: 1834 59 76 512 3478 Fragments arranged by size: 3478 1834 512 76 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2347 2625 2659 2794 2855 2347 Size: 278 34 135 61 5451 Fragments arranged by size: 5451 278 135 61 34 SacI G_AGCT'C Cuts at: 2741 2741 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 3215 3215 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2660 2719 2795 3307 4442 5123 5314 Size: 219 1615 59 76 512 1135 681 191 Cuts at: 5314 826 Size: 1471 Fragments arranged by size: 1615 1471 1135 681 512 219 191 76 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 2958 4063 4964 5226 820 Size: 197 926 456 559 1105 901 262 1553 Fragments arranged by size: 1553 1105 926 901 559 456 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2406 3398 513 Size: 552 1341 992 3074 Fragments arranged by size: 3074 1341 992 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 2667 3986 4003 4156 4341 4786 Size: 1255 1067 20 1319 17 153 185 445 Cuts at: 4786 325 Size: 1498 Fragments arranged by size: 1498 1319 1255 1067 445 185 153 20 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2094 4205 5917 Size: 274 80 33 51 1443 117 2111 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 2111 1712 1443 274 138 117 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3044 3295 3044 Size: 251 5708 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2054 2351 2054 Size: 297 5662 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 2025 2939 4088 2025 Size: 914 1149 3896 Fragments arranged by size: 3896 1149 914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI