Restriction map for pFN21AB0498
 (Circular) (Six-base) MAPSORT of: pFN21AB0498.seq  Check: 8509  from: 1  to: 5959



pFN21AB0498  5959 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2656    2656 
   Size:       5959
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3769     278 
   Size:         53      83     186     936    2233    2468

  Fragments arranged by size: 

                2468    2233     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3302    1937 
   Size:       1365    4594
 
Acc65I G'GTAC_C

Cuts at:   3280    3280 
   Size:       5959
 
AclI AA'CG_TT

Cuts at:   1563    4086    4459    1563 
   Size:       2523     373    3063

  Fragments arranged by size: 

                3063    2523     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2369    2477    4022    5406    5674    1632 
   Size:        737     108    1545    1384     268    1917

  Fragments arranged by size: 

                1917    1545    1384     737     268     108
 
AfeI AGC'GCT

Cuts at:   3181    3181 
   Size:       5959
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5762
 
AflIII A'CryG_T

Cuts at:   1203    4858    5835    1203 
   Size:       3655     977    1327

  Fragments arranged by size: 

                3655    1327     977
 
AgeI A'CCGG_T

Cuts at:   4790    4790 
   Size:       5959
 
AhdI GACnn_n'nnGTC

Cuts at:   4688    4688 
   Size:       5959
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5959
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5959
 
AlwNI CAG_nnn'CTG

Cuts at:   2143    3702    5274    2143 
   Size:       1559    1572    2828

  Fragments arranged by size: 

                2828    1572    1559
 
ApaI G_GGCC'C

Cuts at:   2150    2150 
   Size:       5959
 
ApaLI G'TGCA_C

Cuts at:   4016    5172    4016 
   Size:       1156    4803
 
ApoI r'AATT_y

Cuts at:   1493    3268    3494    3646    5755    1493 
   Size:       1775     226     152    2109    1697

  Fragments arranged by size: 

                2109    1775    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2220    2966    4513     160 
   Size:        872    1188     746    1547    1606

  Fragments arranged by size: 

                1606    1547    1188     872     746
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5959
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3284    1870 
   Size:         73    1341    4545

  Fragments arranged by size: 

                4545    1341      73
 
BamHI G'GATC_C

Cuts at:   3289    3656    3289 
   Size:        367    5592
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3280    4735     618 
   Size:        325     217     627    1493    1455    1842

  Fragments arranged by size: 

                1842    1493    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2150    2467    2741    3278    1391 
   Size:        759     317     274     537    4072

  Fragments arranged by size: 

                4072     759     537     317     274
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2988     928 
   Size:        971    1089    3899

  Fragments arranged by size: 

                3899    1089     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2816    2850    4150    4184    1903 
   Size:         34     879      34    1300      34    3678

  Fragments arranged by size: 

                3678    1300     879      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3851    5061    3851 
   Size:       1210    4749
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5959
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4570     136 
   Size:        107     122      71     631     605    2898    1525

  Fragments arranged by size: 

                2898    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2587    5954    2587 
   Size:       3367    2592
 
BlpI GC'TnA_GC

Cuts at:   3376    3376 
   Size:       5959
 
Bme1580I G_kGCm'C

Cuts at:   1790    2150    2260    4020    5176    1790 
   Size:        360     110    1760    1156    2573

  Fragments arranged by size: 

                2573    1760    1156     360     110
 
BmgBI CAC'GTC

Cuts at:   1318    2039    1318 
   Size:        721    5238
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3214    3690    4648 
   Size:        416      53      83     399      24    1797     476     958

Cuts at:   4648     442 
   Size:       1753

  Fragments arranged by size: 

                1797    1753     958     476     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5959
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2951    4619    1239 
   Size:       1712    1668    2579

  Fragments arranged by size: 

                2579    1712    1668
 
Bpu10I CC'TnA_GC

Cuts at:   2735    2735 
   Size:       5959
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2384    2943    4084    4949    5247    2384 
   Size:        559    1141     865     298    3096

  Fragments arranged by size: 

                3096    1141     865     559     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4622     882 
   Size:        617    3123    2219

  Fragments arranged by size: 

                3123    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2317    3750     493 
   Size:        711    1113    1433    2702

  Fragments arranged by size: 

                2702    1433    1113     711
 
BsaBI GATnn'nnATC

Cuts at:   5953    5953 
   Size:       5959
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3766    4148    4783     275 
   Size:         53      83     186     936    2233     382     635    1451

  Fragments arranged by size: 

                2233    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4392    4790    5064    5211    5622    4392 
   Size:        398     274     147     411    4729

  Fragments arranged by size: 

                4729     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2708    2738    1178 
   Size:         30     165      30    1305      30    4399

  Fragments arranged by size: 

                4399    1305     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2621    1186 
   Size:       1435    4524
 
BseYI C'CCAG_C

Cuts at:   2143    2253    2687    3055    5162    5658    2143 
   Size:        110     434     368    2107     496    2444

  Fragments arranged by size: 

                2444    2107     496     434     368     110
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5386
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2633    3409    4170    5198    1540 
   Size:        275     186     632     776     761    1028    2301

  Fragments arranged by size: 

                2301    1028     776     761     632     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2741    4020    4105    5176    2741 
   Size:       1279      85    1071    3524

  Fragments arranged by size: 

                3524    1279    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2488    3470    3563    2488 
   Size:        982      93    4884

  Fragments arranged by size: 

                4884     982      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2150    2260    2467    2741    3278    4020    4105 
   Size:        399     360     110     207     274     537     742      85

Cuts at:   4105    5176    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     537     399     360     274     207
                 110      85
 
BspEI T'CCGG_A

Cuts at:   5622    5622 
   Size:       5959
 
BspHI T'CATG_A

Cuts at:   2365    3848    5578    2365 
   Size:       1483    1730    2746

  Fragments arranged by size: 

                2746    1730    1483
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2656    2821    3314     844 
   Size:       1812     165     493    3489

  Fragments arranged by size: 

                3489    1812     493     165
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2815    3846    1394 
   Size:        429     992    1031    3507

  Fragments arranged by size: 

                3507    1031     992     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4454    4628      65 
   Size:       1183    3206     174    1396

  Fragments arranged by size: 

                3206    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2704    4603    4790    1700 
   Size:        110     894    1899     187    2869

  Fragments arranged by size: 

                2869    1899     894     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5959
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5959
 
BssSI C'ACGA_G

Cuts at:   4019    5031    4019 
   Size:       1012    4947
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5959
 
BstBI TT'CG_AA

Cuts at:   4763    4763 
   Size:       5959
 
BstEII G'GTnAC_C

Cuts at:   1184    2668    3097    1184 
   Size:       1484     429    4046

  Fragments arranged by size: 

                4046    1484     429
 
BstYI r'GATC_y

Cuts at:   1972    2587    3155    3289    3656    4041    4058    5510    5596 
   Size:        615     568     134     367     385      17    1452      86

Cuts at:   5596    5608    5954    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     615     568     385     367     346     134
                  86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2132    2468    2132 
   Size:        336    5623
 
BtgI C'CryG_G

Cuts at:    513    1065    2748     513 
   Size:        552    1683    3724

  Fragments arranged by size: 

                3724    1683     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5220

  Fragments arranged by size: 

                5220     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3475    4269    4289    3475 
   Size:        794      20    5145

  Fragments arranged by size: 

                5145     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5959
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2529    2564     529 
   Size:         35    1965      35    3924

  Fragments arranged by size: 

                3924    1965      35      35
 
DraI TTT'AAA

Cuts at:   3263    3263 
   Size:       5959
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4966     809 
   Size:        507    3650    1802

  Fragments arranged by size: 

                3650    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2226    3406    4295 
   Size:         54    1425      50     275      18     396    1180     889

Cuts at:   4295    5924       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1180     889     396     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3406    1537 
   Size:        275    1594    4090

  Fragments arranged by size: 

                4090    1594     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2100    2573    2855    3215    3889    1353 
   Size:        747     473     282     360     674    3423

  Fragments arranged by size: 

                3423     747     674     473     360     282
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2193    2723    2734    4546    4920    5066 
   Size:       1064     138     604     530      11    1812     374     146

Cuts at:   5066     387 
   Size:       1280

  Fragments arranged by size: 

                1812    1280    1064     604     530     374     146     138
                  11
 
EcoICRI GAG'CTC

Cuts at:   2739    2739 
   Size:       5959
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2369    2477    2951    4022    4619    5406    5674 
   Size:        393     737     108     474    1071     597     787     268

Cuts at:   5674    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1071     787     737     597     474     393     268
                 108
 
EcoO109I rG'GnC_Cy

Cuts at:   2128    2395    3163    2128 
   Size:        267     768    4924

  Fragments arranged by size: 

                4924     768     267
 
EcoRI G'AATT_C

Cuts at:   3268    3646    3268 
   Size:        378    5581
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2045    2960    2045 
   Size:        915    5044
 
FspI TGC'GCA

Cuts at:   4465    4465 
   Size:       5959
 
HaeII r_GCGC'y

Cuts at:   1678    2876    3183    5106    1678 
   Size:       1198     307    1923    2531

  Fragments arranged by size: 

                2531    1923    1198     307
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2572    2604    2708    2740    4600 
   Size:         32     135      32    1000      32     104      32    1860

Cuts at:   4600    4632    4674    4706    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1860    1000     135     104      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2293    3303    3549     669 
   Size:        988     281     355    1010     246    3079

  Fragments arranged by size: 

                3079    1010     988     355     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2534     748 
   Size:       1786    4173
 
HpaI GTT'AAC

Cuts at:   3549    3549 
   Size:       5959
 
KpnI G_GTAC'C

Cuts at:   3284    3284 
   Size:       5959
 
MfeI C'AATT_G

Cuts at:   3558    3558 
   Size:       5959
 
MluI A'CGCG_T

Cuts at:   5835    5835 
   Size:       5959
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5073    5257    1305 
   Size:       3768     184    2007

  Fragments arranged by size: 

                3768    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2228      10 
   Size:         54    1425     343     396    3741

  Fragments arranged by size: 

                3741    1425     396     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2370    3917    4276    4435     518 
   Size:        648    1204    1547     359     159    2042

  Fragments arranged by size: 

                2042    1547    1204     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2347    2625    2659    2773    2794    2855    3375    4052 
   Size:        741     278      34     114      21      61     520     677

Cuts at:   4052    5200    5445    1606 
   Size:       1148     245    2120

  Fragments arranged by size: 

                2120    1148     741     677     520     278     245     114
                  61      34      21
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5407
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5959
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5959
 
NotI GC'GGCC_GC

Cuts at:   3406    3406 
   Size:       5959
 
NspI r_CATG'y

Cuts at:   1132    1229    3317    4862    1132 
   Size:         97    2088    1545    2229

  Fragments arranged by size: 

                2229    2088    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2644    1348 
   Size:       1296    4663
 
PciI A'CATG_T

Cuts at:   4858    4858 
   Size:       5959
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5959
 
PfoI T'CCnGG_A

Cuts at:   2808    2928    2808 
   Size:        120    5839
 
PmeI GTTT'AAAC

Cuts at:   3263    3263 
   Size:       5959
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4028    4060    5541    5573    4028 
   Size:         32    1481      32    4414

  Fragments arranged by size: 

                4414    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   3163    3163 
   Size:       5959
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5959
 
PsiI TTA'TAA

Cuts at:   3529    3529 
   Size:       5959
 
PspOMI G'GGCC_C

Cuts at:   2146    2146 
   Size:       5959
 
PstI C_TGCA'G

Cuts at:    830    2664    2723    2799    3311     830 
   Size:       1834      59      76     512    3478

  Fragments arranged by size: 

                3478    1834     512      76      59
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5959
 
PvuII CAG'CTG

Cuts at:   2347    2625    2659    2794    2855    2347 
   Size:        278      34     135      61    5451

  Fragments arranged by size: 

                5451     278     135      61      34
 
SacI G_AGCT'C

Cuts at:   2741    2741 
   Size:       5959
 
SalI G'TCGA_C

Cuts at:   1936    3301    1936 
   Size:       1365    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3215    3215 
   Size:       5959
 
SbfI CC_TGCA'GG

Cuts at:   3311    3311 
   Size:       5959
 
ScaI AGT'ACT

Cuts at:   4207    5919    4207 
   Size:       1712    4247
 
SfcI C'TryA_G

Cuts at:    826    1045    2660    2719    2795    3307    4442    5123    5314 
   Size:        219    1615      59      76     512    1135     681     191

Cuts at:   5314     826 
   Size:       1471

  Fragments arranged by size: 

                1615    1471    1135     681     512     219     191      76
                  59
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5959
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5959
 
SmaI CCC'GGG

Cuts at:   1872    3286    1872 
   Size:       1414    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2399    2958    4063    4964    5226     820 
   Size:        197     926     456     559    1105     901     262    1553

  Fragments arranged by size: 

                1553    1105     926     901     559     456     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3750     493 
   Size:       3257    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5959
 
SphI G_CATG'C

Cuts at:   1132    3317    1132 
   Size:       2185    3774
 
SspI AAT'ATT

Cuts at:      5      52    3883       5 
   Size:         47    3831    2081

  Fragments arranged by size: 

                3831    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5959
 
StyI C'CwwG_G

Cuts at:    513    1065    2406    3398     513 
   Size:        552    1341     992    3074

  Fragments arranged by size: 

                3074    1341     992     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2647    2667    3986    4003    4156    4341    4786 
   Size:       1255    1067      20    1319      17     153     185     445

Cuts at:   4786     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1319    1255    1067     445     185     153      20
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2094    4205    5917 
   Size:        274      80      33      51    1443     117    2111    1712

Cuts at:   5917      96 
   Size:        138

  Fragments arranged by size: 

                2111    1712    1443     274     138     117      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5919    5951    5919 
   Size:         32    5927
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5959
 
XbaI T'CTAG_A

Cuts at:   3044    3295    3044 
   Size:        251    5708
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2054    2351    2054 
   Size:        297    5662
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5959
 
XmaI C'CCGG_G

Cuts at:   1870    3284    1870 
   Size:       1414    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2025    2939    4088    2025 
   Size:        914    1149    3896

  Fragments arranged by size: 

                3896    1149     914
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3767     276 
   Size:         53      83     186     936    2233    2468

  Fragments arranged by size: 

                2468    2233     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AloI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI     BplI
    BsiWI    BsmBI    BstXI  BstZ17I   DraIII    EcoNI    EcoRV     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI
     PmlI    PspXI     PsrI    RsrII    SacII    SanDI    SexAI     SfiI
     SfoI     SrfI     SwaI
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