Restriction map for
pFN21AB0498
(Linear) (Six-base) MAPSORT of: KIBB0498.seq Check: 7266 from: 1 to: 1254
KIBB0498.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 651 1254
Size: 651 603
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 472 1254
Size: 364 108 782
Fragments arranged by size:
782 364 108
AfeI AGC'GCT
Cuts at: 0 1176 1254
Size: 1176 78
AlwNI CAG_nnn'CTG
Cuts at: 0 138 1254
Size: 138 1116
ApaI G_GGCC'C
Cuts at: 0 145 1254
Size: 145 1109
AseI AT'TA_AT
Cuts at: 0 215 961 1254
Size: 215 746 293
Fragments arranged by size:
746 293 215
BanII G_rGCy'C
Cuts at: 0 145 462 736 1254
Size: 145 317 274 518
Fragments arranged by size:
518 317 274 145
BbsI GAAGACnn'nnnn_
Cuts at: 0 983 1254
Size: 983 271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 811 845 1254
Size: 811 34 409
Fragments arranged by size:
811 409 34
BglII A'GATC_T
Cuts at: 0 582 1254
Size: 582 672
Bme1580I G_kGCm'C
Cuts at: 0 145 255 1254
Size: 145 110 999
Fragments arranged by size:
999 145 110
BmgBI CAC'GTC
Cuts at: 0 34 1254
Size: 34 1220
BmrI ACTGGGnnnn_n'
Cuts at: 0 1209 1254
Size: 1209 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1254
Size: 946 308
Bpu10I CC'TnA_GC
Cuts at: 0 730 1254
Size: 730 524
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 938 1254
Size: 379 559 316
Fragments arranged by size:
559 379 316
BsaAI yAC'GTr
Cuts at: 0 312 1254
Size: 312 942
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 703 733 1254
Size: 703 30 521
Fragments arranged by size:
703 521 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 616 1254
Size: 616 638
BseYI C'CCAG_C
Cuts at: 0 138 248 682 1050 1254
Size: 138 110 434 368 204
Fragments arranged by size:
434 368 204 138 110
BsiEI CG_ry'CG
Cuts at: 0 628 1254
Size: 628 626
BsiHKAI G_wGCw'C
Cuts at: 0 736 1254
Size: 736 518
BsmI GAATG_Cn'
Cuts at: 0 483 1254
Size: 483 771
Bsp1286I G_dGCh'C
Cuts at: 0 145 255 462 736 1254
Size: 145 110 207 274 518
Fragments arranged by size:
518 274 207 145 110
BspHI T'CATG_A
Cuts at: 0 360 1254
Size: 360 894
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 651 816 1254
Size: 651 165 438
Fragments arranged by size:
651 438 165
BsrBI CCG'CTC
Cuts at: 0 810 1254
Size: 810 444
BsrFI r'CCGG_y
Cuts at: 0 699 1254
Size: 699 555
BstEII G'GTnAC_C
Cuts at: 0 663 1092 1254
Size: 663 429 162
Fragments arranged by size:
663 429 162
BstYI r'GATC_y
Cuts at: 0 582 1150 1254
Size: 582 568 104
Fragments arranged by size:
582 568 104
Bsu36I CC'TnA_GG
Cuts at: 0 127 463 1254
Size: 127 336 791
Fragments arranged by size:
791 336 127
BtgI C'CryG_G
Cuts at: 0 743 1254
Size: 743 511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 524 559 1254
Size: 524 35 695
Fragments arranged by size:
695 524 35
EaeI y'GGCC_r
Cuts at: 0 221 1254
Size: 221 1033
EarI CTCTTCn'nnn_
Cuts at: 0 95 568 850 1210 1254
Size: 95 473 282 360 44
Fragments arranged by size:
473 360 282 95 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 188 718 729 1254
Size: 188 530 11 525
Fragments arranged by size:
530 525 188 11
EcoICRI GAG'CTC
Cuts at: 0 734 1254
Size: 734 520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 472 946 1254
Size: 364 108 474 308
Fragments arranged by size:
474 364 308 108
EcoO109I rG'GnC_Cy
Cuts at: 0 123 390 1158 1254
Size: 123 267 768 96
Fragments arranged by size:
768 267 123 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 40 955 1254
Size: 40 915 299
Fragments arranged by size:
915 299 40
HaeII r_GCGC'y
Cuts at: 0 871 1178 1254
Size: 871 307 76
Fragments arranged by size:
871 307 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 567 599 703 735 1254
Size: 567 32 104 32 519
Fragments arranged by size:
567 519 104 32 32
HincII GTy'rAC
Cuts at: 0 288 1254
Size: 288 966
HindIII A'AGCT_T
Cuts at: 0 529 1254
Size: 529 725
MscI TGG'CCA
Cuts at: 0 223 1254
Size: 223 1031
MslI CAynn'nnrTG
Cuts at: 0 365 1254
Size: 365 889
MspA1I CmG'CkG
Cuts at: 0 342 620 654 768 789 850 1254
Size: 342 278 34 114 21 61 404
Fragments arranged by size:
404 342 278 114 61 34 21
PasI CC'CwG_GG
Cuts at: 0 639 1254
Size: 639 615
PfoI T'CCnGG_A
Cuts at: 0 803 923 1254
Size: 803 120 331
Fragments arranged by size:
803 331 120
PpuMI rG'GwC_Cy
Cuts at: 0 1158 1254
Size: 1158 96
PspOMI G'GGCC_C
Cuts at: 0 141 1254
Size: 141 1113
PstI C_TGCA'G
Cuts at: 0 659 718 794 1254
Size: 659 59 76 460
Fragments arranged by size:
659 460 76 59
PvuII CAG'CTG
Cuts at: 0 342 620 654 789 850 1254
Size: 342 278 34 135 61 404
Fragments arranged by size:
404 342 278 135 61 34
SacI G_AGCT'C
Cuts at: 0 736 1254
Size: 736 518
SapI GCTCTTCn'nnn_
Cuts at: 0 1210 1254
Size: 1210 44
SfcI C'TryA_G
Cuts at: 0 655 714 790 1254
Size: 655 59 76 464
Fragments arranged by size:
655 464 76 59
SmlI C'TyrA_G
Cuts at: 0 394 953 1254
Size: 394 559 301
Fragments arranged by size:
559 394 301
StyI C'CwwG_G
Cuts at: 0 401 1254
Size: 401 853
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 662 1254
Size: 642 20 592
Fragments arranged by size:
642 592 20
TatI w'GTAC_w
Cuts at: 0 89 1254
Size: 89 1165
XbaI T'CTAG_A
Cuts at: 0 1039 1254
Size: 1039 215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 49 346 1254
Size: 49 297 908
Fragments arranged by size:
908 297 49
XmnI GAAnn'nnTTC
Cuts at: 0 20 934 1254
Size: 20 914 320
Fragments arranged by size:
914 320 20
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI AseI BanII BbsI
BcgI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrFI BstEII BstYI Bsu36I BtgI
CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HindIII MscI MslI MspA1I PasI
PfoI PpuMI PspOMI PstI PvuII SacI SapI SfcI
SmlI StyI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI
BfrBI BglI BlpI BmtI BplI BsaI BsaBI BsaHI
BsaWI BsgI BsiWI BsmBI BspEI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI
DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FseI
FspI FspAI HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XhoI XmaI ZraI