################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AvrII BbeI BbrPI BglI BlnI BsaAI BsiWI BspMI BsrBI BsrFI BssHII Bst1107I BstZ17I Cfr10I CpoI EagI Eam1105I EclXI Eco47III Eco52I FseI HincII HindII HpaI KasI KspI MluI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 6809 GGTACC G^GTACC AccI 1174 GTAGAC GT^[AC][GT]AC AccII 5593 CGCG CG^CG AccIII 727 TCCGGA T^CCGGA AflIII 6443 ACATGT A^C[GA][CT]GT Asp700I 896 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 6809 GGTACC G^GTACC AvaI 4940 CTCGGG C^[CT]CG[GA]G BseAI 727 TCCGGA T^CCGGA BsiEI 7049 CGGTCG CG[GA][CT]^CG BsmBI 1863 GAGACG CGTCTC(1/5) BsoBI 4940 CTCGGG C^[CT]CG[GA]G BspDI 4214 ATCGAT AT^CGAT BspEI 727 TCCGGA T^CCGGA BspLU11I 6443 ACATGT A^CATGT BssSI 5821 CTCGTG CACGAG(-5/-1) BstBI 4901 TTCGAA TT^CGAA BstUI 5593 CGCG CG^CG ClaI 4214 ATCGAT AT^CGAT EcoRI 402 GAATTC G^AATTC EcoRV 6730 GATATC GAT^ATC FauI 4087 GCGGG CCCGC(4/6) HaeII 6210 AGCGCC [GA]GCGC^[CT] KpnI 6809 GGTACC GGTAC^C MroI 727 TCCGGA T^CCGGA MvnI 5593 CGCG CG^CG NdeI 5084 CATATG CA^TATG NheI 2098 GCTAGC G^CTAGC NspV 4901 TTCGAA TT^CGAA SacI 756 GAGCTC GAGCT^C SexAI 44 ACCTGGT A^CC[AT]GGT SfuI 4901 TTCGAA TT^CGAA SphI 1617 GCATGC GCATG^C SspI 6203 AATATT AAT^ATT XbaI 2977 TCTAGA T^CTAGA XmnI 896 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 490 GACGTC GACGT^C 2084 GACGTC AcyI 490 GACGTC G[GA]^CG[CT]C 2084 GACGTC AflII 619 CTTAAG C^TTAAG 4746 CTTAAG AocI 121 CCTGAGG CC^T[ACGT]AGG 5526 CCTGAGG AspI 267 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1865 GACGGTGTC BamHI 713 GGATCC G^GATCC 2824 GGATCC BanII 756 GAGCTC G[GA]GC[CT]^C 3253 GAGCCC BbvCI 4406 CCTCAGC CCTCAGC(-5/-2) 1371 GCTGAGG BclI 1656 TGATCA T^GATCA 3587 TGATCA BfrI 619 CTTAAG C^TTAAG 4746 CTTAAG BlpI 3784 GCTGAGC GC^T[ACGT]AGC 5728 GCTGAGC Bpu1102I 3784 GCTGAGC GC^T[ACGT]AGC 5728 GCTGAGC BsaHI 490 GACGTC G[GA]^CG[CT]C 2084 GACGTC BsaI 3487 GAGACC GGTCTC(1/5) 5090 GGTCTC BsaWI 727 TCCGGA [AT]^CCGG[AT] 6806 TCCGGT BstAPI 1485 GCATACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4155 GCAGTGTGTGC BstEII 3102 GGTAACC G^GT[ACGT]ACC 3144 GGTCACC BstPI 3102 GGTAACC G^GT[ACGT]ACC 3144 GGTCACC BstXI 3748 CCAAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6934 CCACATCCTTGG Bsu36I 121 CCTGAGG CC^T[ACGT]AGG 5526 CCTGAGG CelII 3784 GCTGAGC GC^T[ACGT]AGC 5728 GCTGAGC DraIII 1769 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG 5115 CACAAGGTG Eco81I 121 CCTGAGG CC^T[ACGT]AGG 5526 CCTGAGG EcoNI 3888 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 6255 CCTCTACAAGG EcoO65I 3102 GGTAACC G^GT[ACGT]ACC 3144 GGTCACC FbaI 1656 TGATCA T^GATCA 3587 TGATCA Hin1I 490 GACGTC G[GA]^CG[CT]C 2084 GACGTC NcoI 2015 CCATGG C^CATGG 5095 CCATGG PflFI 267 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1865 GACGGTGTC PpuMI 536 AGGTCCT [GA]G^G[AT]CC[CT] 5879 AGGTCCT PshAI 2969 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4744 GACTTAAGTC Tth111I 267 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1865 GACGGTGTC XcmI 558 CCATCTCCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3745 CCACCAAACCCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII AviII BalI BanI BbsI BbvI BcgI BciVI BcnI BfaI BglII BmyI BpmI BpuAI BsaBI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PshBI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII