(Circular) (Six-base) MAPSORT of: pFN21AB9290.seq Check: 790 from: 1 to: 5596 pFN21AB9290 5596 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 2484 3723 4096 1563 Size: 921 1239 373 3063 Fragments arranged by size: 3063 1239 921 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 2226 2702 3659 5043 5311 1632 Size: 521 73 476 957 1384 268 1917 Fragments arranged by size: 1917 1384 957 521 476 268 73 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5596 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2121 2153 2121 Size: 32 5564 AlwNI CAG_nnn'CTG Cuts at: 2059 2467 2602 3339 4911 2059 Size: 408 135 737 1572 2744 Fragments arranged by size: 2744 1572 737 408 135 ApaLI G'TGCA_C Cuts at: 2118 3653 4809 2118 Size: 1535 1156 2905 Fragments arranged by size: 2905 1535 1156 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2445 2921 1870 Size: 73 502 476 4545 Fragments arranged by size: 4545 502 476 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2795 2917 4372 618 Size: 325 217 627 1008 122 1455 1842 Fragments arranged by size: 1842 1455 1008 627 325 217 122 BanII G_rGCy'C Cuts at: 1391 2774 2915 1391 Size: 1383 141 4072 Fragments arranged by size: 4072 1383 141 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4207 136 Size: 107 122 71 631 605 2535 1525 Fragments arranged by size: 2535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2172 5591 2172 Size: 3419 2177 BlpI GC'TnA_GC Cuts at: 2391 3013 2391 Size: 622 4974 Bme1580I G_kGCm'C Cuts at: 1790 2122 2566 3657 4813 1790 Size: 332 444 1091 1156 2573 Fragments arranged by size: 2573 1156 1091 444 332 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2215 2565 3327 Size: 416 53 83 399 24 798 350 762 Cuts at: 3327 4285 442 Size: 958 1753 Fragments arranged by size: 1753 958 798 762 416 399 350 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2189 2199 2269 2549 2870 4256 1239 Size: 887 63 10 70 280 321 1386 2579 Fragments arranged by size: 2579 1386 887 321 280 70 63 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3721 4586 4884 3721 Size: 865 298 4433 Fragments arranged by size: 4433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4259 882 Size: 617 2760 2219 Fragments arranged by size: 2760 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2567 3387 493 Size: 711 1363 820 2702 Fragments arranged by size: 2702 1363 820 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3403 3785 4420 275 Size: 53 83 186 936 1870 382 635 1451 Fragments arranged by size: 1870 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2416 4029 4427 4701 4848 5259 2416 Size: 1613 398 274 147 411 2753 Fragments arranged by size: 2753 1613 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2048 2078 2121 2151 2323 Size: 30 165 30 645 30 43 30 172 Cuts at: 2323 2353 2414 2444 1178 Size: 30 61 30 4330 Fragments arranged by size: 4330 645 172 165 61 43 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 1186 Size: 943 4653 BseYI C'CCAG_C Cuts at: 2602 2697 4799 5295 2602 Size: 95 2102 496 2903 Fragments arranged by size: 2903 2102 496 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 1163 Size: 573 432 4591 Fragments arranged by size: 4591 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2734 3046 3807 4835 1540 Size: 275 186 733 312 761 1028 2301 Fragments arranged by size: 2301 1028 761 733 312 275 186 BsiHKAI G_wGCw'C Cuts at: 2122 2232 2392 2808 2856 3657 3742 4813 2122 Size: 110 160 416 48 801 85 1071 2905 Fragments arranged by size: 2905 1071 801 416 160 110 85 48 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2232 2392 2566 2774 2808 2856 Size: 399 332 110 160 174 208 34 48 Cuts at: 2856 2915 3657 3742 4813 1391 Size: 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 332 208 174 160 110 85 59 48 34 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2601 2951 844 Size: 1757 350 3489 Fragments arranged by size: 3489 1757 350 BsrBI CCG'CTC Cuts at: 1394 1823 2736 3483 1394 Size: 429 913 747 3507 Fragments arranged by size: 3507 913 747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2673 2729 4240 4427 1700 Size: 110 863 56 1511 187 2869 Fragments arranged by size: 2869 1511 863 187 110 56 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5596 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2848 2011 Size: 837 4759 BstYI r'GATC_y Cuts at: 1972 2172 2926 3293 3678 3695 5147 5233 5245 Size: 200 754 367 385 17 1452 86 12 Cuts at: 5245 5591 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 754 385 367 346 200 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2863 2863 Size: 5596 BtgI C'CryG_G Cuts at: 513 1065 2709 513 Size: 552 1644 3400 Fragments arranged by size: 3400 1644 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 2120 3112 3906 3926 2120 Size: 992 794 20 3790 Fragments arranged by size: 3790 992 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2731 3043 3932 Size: 54 1425 50 275 18 901 312 889 Cuts at: 3932 5561 8 Size: 1629 43 Fragments arranged by size: 1629 1425 901 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2731 3043 1537 Size: 275 919 312 4090 Fragments arranged by size: 4090 919 312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3526 1353 Size: 2173 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2153 2189 2199 2226 2269 2549 Size: 393 494 27 36 10 27 43 280 Cuts at: 2549 2702 2870 3659 4256 5043 5311 1239 Size: 153 168 789 597 787 268 1524 Fragments arranged by size: 1524 789 787 597 494 393 280 268 168 153 43 36 27 27 10 EcoNI CCTnn'n_nnAGG Cuts at: 2051 2193 2362 2765 2051 Size: 142 169 403 4882 Fragments arranged by size: 4882 403 169 142 EcoO109I rG'GnC_Cy Cuts at: 2186 2210 2217 2255 2845 2186 Size: 24 7 38 590 4937 Fragments arranged by size: 4937 590 38 24 7 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2662 2662 Size: 5596 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 4743 1678 Size: 3065 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2414 2446 4237 Size: 32 135 32 476 32 334 32 1791 Cuts at: 4237 4269 4311 4343 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1791 476 334 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2291 2940 3186 669 Size: 988 281 353 649 246 3079 Fragments arranged by size: 3079 988 649 353 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 2506 3195 2506 Size: 689 4907 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3774 Fragments arranged by size: 3774 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2331 2438 3554 3913 4072 518 Size: 648 843 322 107 1116 359 159 2042 Fragments arranged by size: 2042 1116 843 648 359 322 159 107 MspA1I CmG'CkG Cuts at: 1606 2167 2885 3012 3689 4837 5082 1606 Size: 561 718 127 677 1148 245 2120 Fragments arranged by size: 2120 1148 718 677 561 245 127 NcoI C'CATG_G Cuts at: 513 1065 2709 513 Size: 552 1644 3400 Fragments arranged by size: 3400 1644 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2954 4499 1132 Size: 97 1725 1545 2229 Fragments arranged by size: 2229 1725 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 2602 1108 Size: 1494 4102 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PmlI CAC'GTG Cuts at: 2567 2567 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2121 2153 3665 3697 5178 5210 2121 Size: 32 1512 32 1481 32 2507 Fragments arranged by size: 2507 1512 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2186 2210 2217 2255 2186 Size: 24 7 38 5527 Fragments arranged by size: 5527 38 24 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PstI C_TGCA'G Cuts at: 830 2948 830 Size: 2118 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2167 2885 2167 Size: 718 4878 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SexAI A'CCwGG_T Cuts at: 2496 2496 Size: 5596 SfcI C'TryA_G Cuts at: 826 1045 2944 4079 4760 4951 826 Size: 219 1899 1135 681 191 1471 Fragments arranged by size: 1899 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2447 2923 1872 Size: 575 476 4545 Fragments arranged by size: 4545 575 476 SmlI C'TyrA_G Cuts at: 820 1017 1943 3700 4601 4863 820 Size: 197 926 1757 901 262 1553 Fragments arranged by size: 1757 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2709 2766 2841 3035 513 Size: 552 1644 57 75 194 3074 Fragments arranged by size: 3074 1644 552 194 75 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2069 2105 2689 2722 3623 3640 3793 Size: 1255 489 36 584 33 901 17 153 Cuts at: 3793 3978 4423 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 901 584 489 445 185 153 36 33 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2302 2323 2334 2355 2697 2729 5556 5588 2302 Size: 21 11 21 342 32 2827 32 2310 Fragments arranged by size: 2827 2310 342 32 32 21 21 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 2074 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2445 2921 1870 Size: 575 476 4545 Fragments arranged by size: 4545 575 476 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI