(Circular) (Six-base) MAPSORT of: pFN21AE1020.seq Check: 1121 from: 1 to: 6709 pFN21AE1020 6709 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3724 3826 3724 Size: 102 6607 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4519 278 Size: 53 83 186 936 2983 2468 Fragments arranged by size: 2983 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 2964 4052 1937 Size: 831 196 1088 4594 Fragments arranged by size: 4594 1088 831 196 Acc65I G'GTAC_C Cuts at: 3956 4030 3956 Size: 74 6635 AclI AA'CG_TT Cuts at: 1563 4836 5209 1563 Size: 3273 373 3063 Fragments arranged by size: 3273 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2140 2512 3246 3376 3505 3615 4772 6156 Size: 508 372 734 130 129 110 1157 1384 Cuts at: 6156 6424 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1157 734 508 372 268 130 129 110 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6512 AflIII A'CryG_T Cuts at: 1203 2967 5608 6585 1203 Size: 1764 2641 977 1327 Fragments arranged by size: 2641 1764 1327 977 AgeI A'CCGG_T Cuts at: 3273 5540 3273 Size: 2267 4442 AhdI GACnn_n'nnGTC Cuts at: 2983 5438 2983 Size: 2455 4254 AleI CACnn'nnGTG Cuts at: 1166 2282 1166 Size: 1116 5593 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6709 AlwNI CAG_nnn'CTG Cuts at: 2104 2467 2535 4452 6024 2104 Size: 363 68 1917 1572 2789 Fragments arranged by size: 2789 1917 1572 363 68 ApaI G_GGCC'C Cuts at: 2512 3158 3710 2512 Size: 646 552 5511 Fragments arranged by size: 5511 646 552 ApaLI G'TGCA_C Cuts at: 4766 5922 4766 Size: 1156 5553 ApoI r'AATT_y Cuts at: 1493 2723 4018 4244 4396 6505 1493 Size: 1230 1295 226 152 2109 1697 Fragments arranged by size: 2109 1697 1295 1230 226 152 AseI AT'TA_AT Cuts at: 160 1032 5263 160 Size: 872 4231 1606 Fragments arranged by size: 4231 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6709 AvaI C'yCGr_G Cuts at: 1870 1943 2373 2502 3314 3336 3372 3703 4034 Size: 73 430 129 812 22 36 331 331 Cuts at: 4034 1870 Size: 4545 Fragments arranged by size: 4545 812 430 331 331 129 73 36 22 BamHI G'GATC_C Cuts at: 4039 4406 4039 Size: 367 6342 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3183 3245 3426 3666 3838 Size: 325 217 627 1396 62 181 240 172 Cuts at: 3838 3956 4030 5485 618 Size: 118 74 1455 1842 Fragments arranged by size: 1842 1455 1396 627 325 240 217 181 172 118 74 62 BanII G_rGCy'C Cuts at: 1391 2512 2544 3026 3068 3158 3379 3588 3710 Size: 1121 32 482 42 90 221 209 122 Cuts at: 3710 3823 3908 4008 4028 1391 Size: 113 85 100 20 4072 Fragments arranged by size: 4072 1121 482 221 209 122 113 100 90 85 42 32 20 BbeI G_GCGC'C Cuts at: 3187 3670 3187 Size: 483 6226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5738 BbvCI CC'TCA_GC Cuts at: 2916 3131 3320 3974 2916 Size: 215 189 654 5651 Fragments arranged by size: 5651 654 215 189 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4900 4934 1903 Size: 34 2963 34 3678 Fragments arranged by size: 3678 2963 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4601 5811 4601 Size: 1210 5499 BclI T'GATC_A Cuts at: 1548 1548 Size: 6709 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2507 3120 3153 Size: 107 122 71 631 605 835 613 33 Cuts at: 3153 3855 3864 5320 136 Size: 702 9 1456 1525 Fragments arranged by size: 1525 1456 835 702 631 613 605 122 107 71 33 9 BglII A'GATC_T Cuts at: 3898 6704 3898 Size: 2806 3903 BlpI GC'TnA_GC Cuts at: 2327 4126 2327 Size: 1799 4910 Bme1580I G_kGCm'C Cuts at: 1790 2512 2742 2835 3158 3250 3429 3649 3678 Size: 722 230 93 323 92 179 220 29 Cuts at: 3678 3710 3843 4770 5926 1790 Size: 32 133 927 1156 2573 Fragments arranged by size: 2573 1156 927 722 323 230 220 179 133 93 92 32 29 BmgBI CAC'GTC Cuts at: 1318 2676 2968 1318 Size: 1358 292 5059 Fragments arranged by size: 5059 1358 292 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2666 2822 3251 Size: 416 53 83 399 24 1249 156 429 Cuts at: 3251 3801 4011 4440 5398 442 Size: 550 210 429 958 1753 Fragments arranged by size: 1753 1249 958 550 429 429 416 399 210 156 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6709 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 3893 2528 Size: 1365 5344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2011 2738 3074 3487 3764 3837 3968 5369 Size: 772 727 336 413 277 73 131 1401 Cuts at: 5369 1239 Size: 2579 Fragments arranged by size: 2579 1401 772 727 413 336 277 131 73 Bpu10I CC'TnA_GC Cuts at: 2634 2916 3131 3320 3403 3974 2634 Size: 282 215 189 83 571 5369 Fragments arranged by size: 5369 571 282 215 189 83 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 3566 4834 5699 5997 2498 Size: 1068 1268 865 298 3210 Fragments arranged by size: 3210 1268 1068 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2991 5372 882 Size: 617 1492 2381 2219 Fragments arranged by size: 2381 2219 1492 617 BsaAI yAC'GTr Cuts at: 493 1204 2805 3644 4500 493 Size: 711 1601 839 856 2702 Fragments arranged by size: 2702 1601 856 839 711 BsaBI GATnn'nnATC Cuts at: 6703 6703 Size: 6709 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3184 3667 3925 4516 Size: 53 83 186 936 1651 483 258 591 Cuts at: 4516 4898 5533 275 Size: 382 635 1451 Fragments arranged by size: 1651 1451 936 635 591 483 382 258 186 83 53 BsaWI w'CCGG_w Cuts at: 3273 5142 5540 5814 5961 6372 3273 Size: 1869 398 274 147 411 3610 Fragments arranged by size: 3610 1869 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2798 2828 3029 3059 1178 Size: 30 165 30 1395 30 201 30 4828 Fragments arranged by size: 4828 1395 201 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2426 2901 3426 1186 Size: 1240 475 525 4469 Fragments arranged by size: 4469 1240 525 475 BseYI C'CCAG_C Cuts at: 2051 2113 2219 2686 3352 5912 6408 2051 Size: 62 106 467 666 2560 496 2352 Fragments arranged by size: 2560 2352 666 496 467 106 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2656 3696 1163 Size: 573 920 1040 4176 Fragments arranged by size: 4176 1040 920 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4159 4920 5948 1540 Size: 275 186 2158 761 1028 2301 Fragments arranged by size: 2301 2158 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 2316 2393 3823 3908 4770 4855 5926 2133 Size: 183 77 1430 85 862 85 1071 2916 Fragments arranged by size: 2916 1430 1071 862 183 85 85 77 BsmI GAATG_Cn' Cuts at: 2365 4220 4313 2365 Size: 1855 93 4761 Fragments arranged by size: 4761 1855 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3932 3932 Size: 6709 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2316 2393 2512 2544 2742 2835 Size: 399 343 183 77 119 32 198 93 Cuts at: 2835 3026 3068 3158 3250 3379 3429 3588 3649 Size: 191 42 90 92 129 50 159 61 Cuts at: 3649 3678 3710 3823 3843 3908 4008 4028 4770 Size: 29 32 113 20 65 100 20 742 Cuts at: 4770 4855 5926 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 343 198 191 183 159 129 119 113 100 93 92 90 85 77 65 61 50 42 32 32 29 20 20 BspEI T'CCGG_A Cuts at: 6372 6372 Size: 6709 BspHI T'CATG_A Cuts at: 4598 6328 4598 Size: 1730 4979 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2529 2780 3724 3826 4064 844 Size: 1685 251 944 102 238 3489 Fragments arranged by size: 3489 1685 944 251 238 102 BsrBI CCG'CTC Cuts at: 1394 1823 2034 2638 3204 4596 1394 Size: 429 211 604 566 1392 3507 Fragments arranged by size: 3507 1392 604 566 429 211 BsrDI GCAATG_nn' Cuts at: 65 1248 5204 5378 65 Size: 1183 3956 174 1396 Fragments arranged by size: 3956 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 3119 3273 3429 3858 5353 5540 Size: 110 236 1073 154 156 429 1495 187 Cuts at: 5540 1700 Size: 2869 Fragments arranged by size: 2869 1495 1073 429 236 187 156 154 110 BsrGI T'GTAC_A Cuts at: 96 2567 96 Size: 2471 4238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6709 BssSI C'ACGA_G Cuts at: 2615 2932 3043 4769 5781 2615 Size: 317 111 1726 1012 3543 Fragments arranged by size: 3543 1726 1012 317 111 BstAPI GCAn_nnn'nTGC Cuts at: 794 2104 794 Size: 1310 5399 BstBI TT'CG_AA Cuts at: 5513 5513 Size: 6709 BstEII G'GTnAC_C Cuts at: 1184 2149 3008 1184 Size: 965 859 4885 Fragments arranged by size: 4885 965 859 BstYI r'GATC_y Cuts at: 1972 3898 4039 4406 4791 4808 6260 6346 6358 Size: 1926 141 367 385 17 1452 86 12 Cuts at: 6358 6704 1972 Size: 346 1977 Fragments arranged by size: 1977 1926 1452 385 367 346 141 86 17 12 BstZ17I GTA'TAC Cuts at: 2769 2769 Size: 6709 BtgI C'CryG_G Cuts at: 513 1065 2817 2973 3078 3179 3191 3470 513 Size: 552 1752 156 105 101 12 279 3752 Fragments arranged by size: 3752 1752 552 279 156 105 101 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5970 Fragments arranged by size: 5970 683 56 BtsI GCAGTG_nn' Cuts at: 3510 4225 5019 5039 3510 Size: 715 794 20 5180 Fragments arranged by size: 5180 794 715 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6709 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2890 2925 529 Size: 35 2326 35 4313 Fragments arranged by size: 4313 2326 35 35 DraI TTT'AAA Cuts at: 4013 4013 Size: 6709 DraIII CAC_nnn'GTG Cuts at: 3245 3245 Size: 6709 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5716 809 Size: 507 4400 1802 Fragments arranged by size: 4400 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2270 3287 3749 Size: 54 1425 50 275 18 440 1017 462 Cuts at: 3749 3865 3937 4156 5045 6674 8 Size: 116 72 219 889 1629 43 Fragments arranged by size: 1629 1425 1017 889 462 440 275 219 116 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4156 1537 Size: 275 2344 4090 Fragments arranged by size: 4090 2344 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2176 2531 3611 3919 4639 1353 Size: 823 355 1080 308 720 3423 Fragments arranged by size: 3423 1080 823 720 355 308 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2066 3599 5296 5670 5816 387 Size: 1064 138 477 1533 1697 374 146 1280 Fragments arranged by size: 1697 1533 1280 1064 477 374 146 138 EcoICRI GAG'CTC Cuts at: 3821 3906 3821 Size: 85 6624 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2011 2140 2512 2738 3074 3246 3376 Size: 393 379 129 372 226 336 172 130 Cuts at: 3376 3487 3505 3615 3764 3837 3968 4772 5369 Size: 111 18 110 149 73 131 804 597 Cuts at: 5369 6156 6424 1239 Size: 787 268 1524 Fragments arranged by size: 1524 804 787 597 393 379 372 336 268 226 172 149 131 130 129 111 110 73 18 EcoO109I rG'GnC_Cy Cuts at: 2062 2101 2202 2508 2509 2660 3154 3155 3302 Size: 39 101 306 1 151 494 1 147 Cuts at: 3302 3331 3526 3707 3733 3760 2062 Size: 29 195 181 26 27 5011 Fragments arranged by size: 5011 494 306 195 181 151 147 101 39 29 27 26 1 1 EcoRI G'AATT_C Cuts at: 4018 4396 4018 Size: 378 6331 EcoRV GAT'ATC Cuts at: 3786 3786 Size: 6709 FseI GG_CCGG'CC Cuts at: 3862 3862 Size: 6709 FspI TGC'GCA Cuts at: 5215 5215 Size: 6709 HaeII r_GCGC'y Cuts at: 1678 3187 3670 5856 1678 Size: 1509 483 2186 2531 Fragments arranged by size: 2531 2186 1509 483 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2969 3001 3805 3837 3890 Size: 32 135 32 1397 32 804 32 53 Cuts at: 3890 3922 5350 5382 5424 5456 1373 Size: 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 1397 804 135 53 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4053 4299 669 Size: 988 281 2115 246 3079 Fragments arranged by size: 3079 2115 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6709 HpaI GTT'AAC Cuts at: 4299 4299 Size: 6709 KasI G'GCGC_C Cuts at: 3183 3666 3183 Size: 483 6226 KpnI G_GTAC'C Cuts at: 3960 4034 3960 Size: 74 6635 MfeI C'AATT_G Cuts at: 4308 4308 Size: 6709 MluI A'CGCG_T Cuts at: 6585 6585 Size: 6709 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2407 3015 3258 5823 6007 1305 Size: 1102 608 243 2565 184 2007 Fragments arranged by size: 2565 2007 1102 608 243 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3751 10 Size: 54 1425 343 1919 2968 Fragments arranged by size: 2968 1919 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2282 4667 5026 5185 518 Size: 648 978 138 2385 359 159 2042 Fragments arranged by size: 2385 2042 978 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2982 3257 3267 3460 3621 3747 3891 4125 Size: 1376 275 10 193 161 126 144 234 Cuts at: 4125 4802 5950 6195 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1376 1148 677 275 245 234 193 161 144 126 10 NaeI GCC'GGC Cuts at: 2048 3121 3860 2048 Size: 1073 739 4897 Fragments arranged by size: 4897 1073 739 NarI GG'CG_CC Cuts at: 3184 3667 3184 Size: 483 6226 NcoI C'CATG_G Cuts at: 513 1065 2817 2973 513 Size: 552 1752 156 4249 Fragments arranged by size: 4249 1752 552 156 NdeI CA'TA_TG Cuts at: 387 387 Size: 6709 NgoMIV G'CCGG_C Cuts at: 2046 3119 3858 2046 Size: 1073 739 4897 Fragments arranged by size: 4897 1073 739 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6709 NotI GC'GGCC_GC Cuts at: 4156 4156 Size: 6709 NspI r_CATG'y Cuts at: 1132 1229 4067 5612 1132 Size: 97 2838 1545 2229 Fragments arranged by size: 2838 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3017 3294 3727 1348 Size: 1669 277 433 4330 Fragments arranged by size: 4330 1669 433 277 PciI A'CATG_T Cuts at: 5608 5608 Size: 6709 PflMI CCAn_nnn'nTGG Cuts at: 1108 3277 3727 1108 Size: 2169 450 4090 Fragments arranged by size: 4090 2169 450 PfoI T'CCnGG_A Cuts at: 2261 3788 2261 Size: 1527 5182 PmeI GTTT'AAAC Cuts at: 4013 4013 Size: 6709 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4778 4810 6291 6323 4778 Size: 32 1481 32 5164 Fragments arranged by size: 5164 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2202 2660 3302 3331 3526 3733 2202 Size: 458 642 29 195 207 5178 Fragments arranged by size: 5178 642 458 207 195 29 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6709 PsiI TTA'TAA Cuts at: 4279 4279 Size: 6709 PspOMI G'GGCC_C Cuts at: 2508 3154 3706 2508 Size: 646 552 5511 Fragments arranged by size: 5511 646 552 PstI C_TGCA'G Cuts at: 830 2100 2777 3063 3577 4061 830 Size: 1270 677 286 514 484 3478 Fragments arranged by size: 3478 1270 677 514 484 286 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6709 PvuII CAG'CTG Cuts at: 3257 3267 3747 3891 3257 Size: 10 480 144 6075 Fragments arranged by size: 6075 480 144 10 SacI G_AGCT'C Cuts at: 3823 3908 3823 Size: 85 6624 SalI G'TCGA_C Cuts at: 1936 4051 1936 Size: 2115 4594 SanDI GG'GwC_CC Cuts at: 3302 3331 3302 Size: 29 6680 SapI GCTCTTCn'nnn_ Cuts at: 2531 3919 2531 Size: 1388 5321 SbfI CC_TGCA'GG Cuts at: 4061 4061 Size: 6709 ScaI AGT'ACT Cuts at: 4957 6669 4957 Size: 1712 4997 SfcI C'TryA_G Cuts at: 826 1045 2096 2773 3059 3573 4057 5192 5873 Size: 219 1051 677 286 514 484 1135 681 Cuts at: 5873 6064 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1051 681 677 514 484 286 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3153 3864 3153 Size: 711 5998 SfoI GGC'GCC Cuts at: 3185 3668 3185 Size: 483 6226 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6709 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6709 SmaI CCC'GGG Cuts at: 1872 3338 4036 1872 Size: 1466 698 4545 Fragments arranged by size: 4545 1466 698 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 3545 4813 5714 5976 820 Size: 197 926 570 1032 1268 901 262 1553 Fragments arranged by size: 1553 1268 1032 926 901 570 262 197 SnaBI TAC'GTA Cuts at: 493 4500 493 Size: 4007 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6709 SphI G_CATG'C Cuts at: 1132 4067 1132 Size: 2935 3774 SspI AAT'ATT Cuts at: 5 52 4633 5 Size: 47 4581 2081 Fragments arranged by size: 4581 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6709 StyI C'CwwG_G Cuts at: 513 1065 2817 2973 4148 513 Size: 552 1752 156 1175 3074 Fragments arranged by size: 3074 1752 1175 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3233 3286 4736 4753 4906 5091 5536 Size: 1255 1653 53 1450 17 153 185 445 Cuts at: 5536 325 Size: 1498 Fragments arranged by size: 1653 1498 1450 1255 445 185 153 53 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2567 4955 6667 Size: 274 80 33 51 1443 590 2388 1712 Cuts at: 6667 96 Size: 138 Fragments arranged by size: 2388 1712 1443 590 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3029 3061 6669 6701 3029 Size: 32 3608 32 3037 Fragments arranged by size: 3608 3037 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6709 XbaI T'CTAG_A Cuts at: 4045 4045 Size: 6709 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2713 2713 Size: 6709 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6709 XmaI C'CCGG_G Cuts at: 1870 3336 4034 1870 Size: 1466 698 4545 Fragments arranged by size: 4545 1466 698 XmnI GAAnn'nnTTC Cuts at: 4838 4838 Size: 6709 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4517 276 Size: 53 83 186 936 2983 2468 Fragments arranged by size: 2983 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI Bsu36I EcoNI FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SrfI SwaI