(Circular) (Six-base) MAPSORT of: pFN21AB5427.seq Check: 9073 from: 1 to: 5839 pFN21AB5427 5839 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2563 2563 Size: 5839 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3649 278 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3182 1937 Size: 1245 4594 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 3966 4339 1563 Size: 2403 373 3063 Fragments arranged by size: 3063 2403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 2817 3902 5286 5554 1632 Size: 413 772 1085 1384 268 1917 Fragments arranged by size: 1917 1384 1085 772 413 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 4738 5715 1203 Size: 3535 977 1327 Fragments arranged by size: 3535 1327 977 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 4568 4568 Size: 5839 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5839 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5839 AlwNI CAG_nnn'CTG Cuts at: 2038 2980 3130 3582 5154 2038 Size: 942 150 452 1572 2723 Fragments arranged by size: 2723 1572 942 452 150 ApaLI G'TGCA_C Cuts at: 3896 5052 3896 Size: 1156 4683 ApoI r'AATT_y Cuts at: 1493 3148 3374 3526 5635 1493 Size: 1655 226 152 2109 1697 Fragments arranged by size: 2109 1697 1655 226 152 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 2501 2580 3164 1870 Size: 73 558 79 584 4545 Fragments arranged by size: 4545 584 558 79 73 BamHI G'GATC_C Cuts at: 2775 3169 3536 2775 Size: 394 367 5078 Fragments arranged by size: 5078 394 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2879 2902 3160 4615 618 Size: 325 217 627 1092 23 258 1455 1842 Fragments arranged by size: 1842 1455 1092 627 325 258 217 23 BanII G_rGCy'C Cuts at: 1391 2357 3098 3158 1391 Size: 966 741 60 4072 Fragments arranged by size: 4072 966 741 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 2422 2793 2805 2997 3028 928 Size: 971 145 378 371 12 192 31 3739 Fragments arranged by size: 3739 971 378 371 192 145 31 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4030 4064 1903 Size: 34 2093 34 3678 Fragments arranged by size: 3678 2093 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3731 4941 3731 Size: 1210 4629 BclI T'GATC_A Cuts at: 1548 2991 1548 Size: 1443 4396 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2911 4450 136 Size: 107 122 71 631 605 1239 1539 1525 Fragments arranged by size: 1539 1525 1239 631 605 122 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 3256 3256 Size: 5839 Bme1580I G_kGCm'C Cuts at: 1790 2216 2537 2882 2907 3900 5056 1790 Size: 426 321 345 25 993 1156 2573 Fragments arranged by size: 2573 1156 993 426 345 321 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5839 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3570 4528 442 Size: 416 53 83 399 24 2153 958 1753 Fragments arranged by size: 2153 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2341 4499 1239 Size: 980 122 2158 2579 Fragments arranged by size: 2579 2158 980 122 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2161 2237 2588 2677 3964 4829 5127 2161 Size: 76 351 89 1287 865 298 2873 Fragments arranged by size: 2873 1287 865 351 298 89 76 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4502 882 Size: 617 3003 2219 Fragments arranged by size: 3003 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3630 493 Size: 711 2426 2702 Fragments arranged by size: 2702 2426 711 BsaBI GATnn'nnATC Cuts at: 5833 5833 Size: 5839 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2894 3646 4028 4663 Size: 53 83 186 936 1361 752 382 635 Cuts at: 4663 275 Size: 1451 Fragments arranged by size: 1451 1361 936 752 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2142 4272 4670 4944 5091 5502 2142 Size: 2130 398 274 147 411 2479 Fragments arranged by size: 2479 2130 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2192 2222 1178 Size: 30 165 30 789 30 4795 Fragments arranged by size: 4795 789 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2105 2665 2876 1186 Size: 916 3 560 211 4149 Fragments arranged by size: 4149 916 560 211 3 BseYI C'CCAG_C Cuts at: 2725 5042 5538 2725 Size: 2317 496 3026 Fragments arranged by size: 3026 2317 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2810 1163 Size: 573 1074 4192 Fragments arranged by size: 4192 1074 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2816 2961 3900 3985 5056 2816 Size: 145 939 85 1071 3599 Fragments arranged by size: 3599 1071 939 145 85 BsmI GAATG_Cn' Cuts at: 2225 3350 3443 2225 Size: 1125 93 4621 Fragments arranged by size: 4621 1125 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2885 2885 Size: 5839 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2357 2537 2816 2882 2907 2961 Size: 399 426 141 180 279 66 25 54 Cuts at: 2961 3098 3158 3900 3985 5056 1391 Size: 137 60 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 426 399 279 180 141 137 85 66 60 54 25 BspEI T'CCGG_A Cuts at: 2142 5502 2142 Size: 3360 2479 BspHI T'CATG_A Cuts at: 3728 5458 3728 Size: 1730 4109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2563 3194 844 Size: 1719 631 3489 Fragments arranged by size: 3489 1719 631 BsrBI CCG'CTC Cuts at: 1394 1823 3000 3726 1394 Size: 429 1177 726 3507 Fragments arranged by size: 3507 1177 726 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4334 4508 65 Size: 1183 3086 174 1396 Fragments arranged by size: 3086 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4483 4670 1700 Size: 110 2673 187 2869 Fragments arranged by size: 2869 2673 187 110 BsrGI T'GTAC_A Cuts at: 96 2687 96 Size: 2591 3248 BssHII G'CGCG_C Cuts at: 1925 2242 1925 Size: 317 5522 BssSI C'ACGA_G Cuts at: 2927 3899 4911 2927 Size: 972 1012 3855 Fragments arranged by size: 3855 1012 972 BstAPI GCAn_nnn'nTGC Cuts at: 794 2220 794 Size: 1426 4413 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5839 BstXI CCAn_nnnn'nTGG Cuts at: 2365 2365 Size: 5839 BstYI r'GATC_y Cuts at: 1972 2775 3169 3536 3921 3938 5390 5476 5488 Size: 803 394 367 385 17 1452 86 12 Cuts at: 5488 5834 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 803 394 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2074 2074 Size: 5839 BtgI C'CryG_G Cuts at: 513 1065 2004 2898 513 Size: 552 939 894 3454 Fragments arranged by size: 3454 939 894 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5100 Fragments arranged by size: 5100 683 56 BtsI GCAGTG_nn' Cuts at: 2720 3355 4149 4169 2720 Size: 635 794 20 4390 Fragments arranged by size: 4390 794 635 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5804 DraI TTT'AAA Cuts at: 3143 3143 Size: 5839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2367 3018 3286 Size: 54 1425 50 275 18 537 651 268 Cuts at: 3286 4175 5804 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 651 537 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 2144 3769 1353 Size: 755 36 1625 3423 Fragments arranged by size: 3423 1625 755 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4426 4800 4946 387 Size: 1064 138 2837 374 146 1280 Fragments arranged by size: 2837 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2219 2341 2817 3902 4499 5286 Size: 393 413 174 122 476 1085 597 787 Cuts at: 5286 5554 1239 Size: 268 1524 Fragments arranged by size: 1524 1085 787 597 476 413 393 268 174 122 EcoO109I rG'GnC_Cy Cuts at: 2362 2362 Size: 5839 EcoRI G'AATT_C Cuts at: 3148 3526 3148 Size: 378 5461 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2155 2869 2155 Size: 714 5125 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 4986 1678 Size: 3308 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2798 2830 3080 Size: 32 135 32 620 32 574 32 250 Cuts at: 3080 3112 4480 4512 4554 4586 1373 Size: 32 1368 32 42 32 2626 Fragments arranged by size: 2626 1368 620 574 250 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3183 3429 669 Size: 988 281 1245 246 3079 Fragments arranged by size: 3079 1245 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5839 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2741 4953 5137 1305 Size: 1436 2212 184 2007 Fragments arranged by size: 2212 2007 1436 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 3020 10 Size: 54 1425 343 537 651 2829 Fragments arranged by size: 2829 1425 651 537 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 2573 2645 3797 4156 4315 518 Size: 648 1257 150 72 1152 359 159 2042 Fragments arranged by size: 2042 1257 1152 648 359 159 150 72 MspA1I CmG'CkG Cuts at: 1606 2185 2675 3255 3932 5080 5325 1606 Size: 579 490 580 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 580 579 490 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2898 513 Size: 552 939 894 3454 Fragments arranged by size: 3454 939 894 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 2572 3197 4742 1132 Size: 97 1343 625 1545 2229 Fragments arranged by size: 2229 1545 1343 625 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 2980 1108 Size: 1872 3967 PfoI T'CCnGG_A Cuts at: 2490 2490 Size: 5839 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 5421 5453 3908 Size: 32 1481 32 4294 Fragments arranged by size: 4294 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2362 Size: 5839 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5839 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PstI C_TGCA'G Cuts at: 830 2159 3191 830 Size: 1329 1032 3478 Fragments arranged by size: 3478 1329 1032 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 PvuII CAG'CTG Cuts at: 2185 2675 2185 Size: 490 5349 SalI G'TCGA_C Cuts at: 1936 3181 1936 Size: 1245 4594 SapI GCTCTTCn'nnn_ Cuts at: 2108 2144 2108 Size: 36 5803 SbfI CC_TGCA'GG Cuts at: 3191 3191 Size: 5839 ScaI AGT'ACT Cuts at: 2867 4087 5799 2867 Size: 1220 1712 2907 Fragments arranged by size: 2907 1712 1220 SexAI A'CCwGG_T Cuts at: 2046 2046 Size: 5839 SfcI C'TryA_G Cuts at: 826 1045 2155 2869 3187 4322 5003 5194 826 Size: 219 1110 714 318 1135 681 191 1471 Fragments arranged by size: 1471 1135 1110 714 681 318 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 2582 3166 1872 Size: 710 584 4545 Fragments arranged by size: 4545 710 584 SmlI C'TyrA_G Cuts at: 820 1017 1943 2176 2252 2603 2656 3943 4844 Size: 197 926 233 76 351 53 1287 901 Cuts at: 4844 5106 820 Size: 262 1553 Fragments arranged by size: 1553 1287 926 901 351 262 233 197 76 53 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5839 SphI G_CATG'C Cuts at: 1132 2572 3197 1132 Size: 1440 625 3774 Fragments arranged by size: 3774 1440 625 SrfI GCCC'GGGC Cuts at: 2582 2582 Size: 5839 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5839 StyI C'CwwG_G Cuts at: 513 1065 2004 2358 2586 2628 2898 3278 513 Size: 552 939 354 228 42 270 380 3074 Fragments arranged by size: 3074 939 552 380 354 270 228 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3866 3883 4036 4221 4666 325 Size: 1255 2286 17 153 185 445 1498 Fragments arranged by size: 2286 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2687 2865 4085 Size: 274 80 33 51 1443 710 178 1220 Cuts at: 4085 5797 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1220 710 274 178 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3058 3090 5799 5831 3058 Size: 32 2709 32 3066 Fragments arranged by size: 3066 2709 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5839 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2400 2377 Size: 23 5816 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 2580 3164 1870 Size: 710 584 4545 Fragments arranged by size: 4545 710 584 XmnI GAAnn'nnTTC Cuts at: 3968 3968 Size: 5839 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3647 276 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SwaI