Restriction map for
pFN21AB5307
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# NO CUT ENZYMES ( 108 enzymes) #
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AclI AcsI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BamHI BbsI BcgI
BciVI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsiEI BsiWI
Bsp1407I BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT14I FseI FspI HincII HindII
HpaI Ksp632I KspI MfeI MluI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI Tru9I Tsp509I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 275 GGTACC G^GTACC
AccI 1186 GTCTAC GT^[AC][GT]AC
AccIII 1229 TCCGGA T^CCGGA
Aor51HI 134 AGCGCT AGC^GCT
ApaI 83 GGGCCC GGGCC^C
Asp718I 275 GGTACC G^GTACC
BanII 83 GGGCCC G[GA]GC[CT]^C
BbeI 1069 GGCGCC GGCGC^C
BclI 926 TGATCA T^GATCA
BfaI 865 CTAG C^TAG
BglII 969 AGATCT A^GATCT
BsaBI 916 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1145 GGTCTC GGTCTC(1/5)
BsaWI 1229 TCCGGA [AT]^CCGG[AT]
BseAI 1229 TCCGGA T^CCGGA
BseRI 21 GAGGAG GAGGAG(10/8)
BsgI 367 CTGCAC GTGCAG(16/14)
BsmBI 670 CGTCTC CGTCTC(1/5)
BsmI 834 GAATGC GAATGC(1/-1)
BspDI 1207 ATCGAT AT^CGAT
BspEI 1229 TCCGGA T^CCGGA
BsrDI 740 GCAATG GCAATG(2/0)
BstAPI 1158 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 495 GGTGACC G^GT[ACGT]ACC
BstPI 495 GGTGACC G^GT[ACGT]ACC
BstXI 1203 CCACATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1207 ATCGAT AT^CGAT
DraIII 112 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 945 CCGTGG C^C[GA][CT]GG
Eco47III 134 AGCGCT AGC^GCT
EcoO65I 495 GGTGACC G^GT[ACGT]ACC
EcoT22I 1060 ATGCAT ATGCA^T
FbaI 926 TGATCA T^GATCA
HindIII 1224 AAGCTT A^AGCTT
KasI 1069 GGCGCC G^GCGCC
KpnI 275 GGTACC GGTAC^C
MaeI 865 CTAG C^TAG
MamI 916 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 684 GAAGA GAAGA(8/7)
MroI 1229 TCCGGA T^CCGGA
NarI 1069 GGCGCC GG^CGCC
NsiI 1060 ATGCAT ATGCA^T
PleI 858 GAGTC GAGTC(4/5)
SbfI 1164 CCTGCAGG CCTGCA^GG
ScaI 449 AGTACT AGT^ACT
SfoI 1069 GGCGCC GGC^GCC
SmaI 31 CCCGGG CCC^GGG
SmlI 829 CTCAAG C^T[CT][GA]AG
Sse8387I 1164 CCTGCAGG CCTGCA^GG
XcmI 611 CCACGCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 31 CCCGGG C^CCGGG
XmaI 31 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 93 GACGTC GACGT^C
1183 GACGTC
AspHI 269 GTGCTC G[AT]GC[AT]^C
727 GAGCAC
AspI 646 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
976 GACCGGGTC
AvaI 31 CCCGGG C^[CT]CG[GA]G
128 CTCGGG
BanI 275 GGTACC G^G[CT][GA]CC
1069 GGCGCC
BbrPI 115 CACGTG CAC^GTG
370 CACGTG
BbvCI 18 GCTGAGG CCTCAGC(-5/-2)
1006 GCTGAGG
BsiHKAI 269 GTGCTC G[AT]GC[AT]^C
727 GAGCAC
BsmAI 671 GTCTC GTCTC(1/5)
1146 GTCTC
BsoBI 31 CCCGGG C^[CT]CG[GA]G
128 CTCGGG
BspMI 278 ACCTGC ACCTGC(4/8)
1190 ACCTGC
BstYI 935 GGATCT [GA]^GATC[CT]
969 AGATCT
MflI 935 GGATCT [GA]^GATC[CT]
969 AGATCT
NspI 602 GCATGT [GA]CATG^[CT]
1058 ACATGC
PflFI 646 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
976 GACCGGGTC
PmaCI 115 CACGTG CAC^GTG
370 CACGTG
PmlI 115 CACGTG CAC^GTG
370 CACGTG
TfiI 596 GAATC G^A[AT]TC
956 GAATC
TspRI 296 CAGTG CA[GC]TG[ACGT][ACGT]^
803 CACTG
Tth111I 646 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
976 GACCGGGTC
XhoII 935 GGATCT [GA]^GATC[CT]
969 AGATCT
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaII BalI BbvI
BcnI BmyI BsaAI BsaHI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TthHB8I