(Circular) (Six-base) MAPSORT of: pFN21AB5307.seq Check: 1289 from: 1 to: 5941 pFN21AB5307 5941 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2102 3192 3751 278 Size: 53 83 186 936 566 1090 559 2468 Fragments arranged by size: 2468 1090 936 566 559 186 83 53 AccI GT'mk_AC Cuts at: 1937 3192 3284 1937 Size: 1255 92 4594 Fragments arranged by size: 4594 1255 92 Acc65I G'GTAC_C Cuts at: 2280 3262 2280 Size: 982 4959 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4004 5388 5656 1632 Size: 2372 1384 268 1917 Fragments arranged by size: 2372 1917 1384 268 AfeI AGC'GCT Cuts at: 2141 2141 Size: 5941 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 2410 2479 3169 3684 5256 2410 Size: 69 690 515 1572 3095 Fragments arranged by size: 3095 1572 690 515 69 ApaI G_GGCC'C Cuts at: 2092 2092 Size: 5941 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2133 3266 1870 Size: 73 93 97 1133 4545 Fragments arranged by size: 4545 1133 97 93 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2280 3074 3262 4717 618 Size: 325 217 627 493 794 188 1455 1842 Fragments arranged by size: 1842 1455 794 627 493 325 217 188 BanII G_rGCy'C Cuts at: 1391 2092 3260 1391 Size: 701 1168 4072 Fragments arranged by size: 4072 1168 701 BbeI G_GCGC'C Cuts at: 3078 3078 Size: 5941 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4970 BbvCI CC'TCA_GC Cuts at: 2024 3012 2024 Size: 988 4953 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 2931 1548 Size: 1383 4558 BfrBI ATG'CAT Cuts at: 3067 3067 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4552 136 Size: 107 122 71 631 605 2880 1525 Fragments arranged by size: 2880 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2974 5936 2974 Size: 2962 2979 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2092 4002 5158 1790 Size: 302 1910 1156 2573 Fragments arranged by size: 2573 1910 1156 302 BmgBI CAC'GTC Cuts at: 1318 3120 1318 Size: 1802 4139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2446 3073 3672 Size: 416 53 83 399 24 1029 627 599 Cuts at: 3672 4630 442 Size: 958 1753 Fragments arranged by size: 1753 1029 958 627 599 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2218 2328 2218 Size: 110 5831 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4601 1239 Size: 3362 2579 Bpu10I CC'TnA_GC Cuts at: 2024 3012 2024 Size: 988 4953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2819 4066 4931 5229 2819 Size: 1247 865 298 3531 Fragments arranged by size: 3531 1247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3156 4604 882 Size: 617 1657 1448 2219 Fragments arranged by size: 2219 1657 1448 617 BsaAI yAC'GTr Cuts at: 493 1204 2122 2377 2525 3732 493 Size: 711 918 255 148 1207 2702 Fragments arranged by size: 2702 1207 918 711 255 148 BsaBI GATnn'nnATC Cuts at: 2925 5935 2925 Size: 3010 2931 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2099 2722 3075 Size: 53 83 186 936 482 84 623 353 Cuts at: 3075 3189 3748 4130 4765 275 Size: 114 559 382 635 1451 Fragments arranged by size: 1451 936 635 623 559 482 382 353 186 114 84 83 53 BsaWI w'CCGG_w Cuts at: 3234 4374 4772 5046 5193 5604 3234 Size: 1140 398 274 147 411 3571 Fragments arranged by size: 3571 1140 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2067 2097 1178 Size: 30 165 30 664 30 5022 Fragments arranged by size: 5022 664 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2041 1186 Size: 855 5086 BseYI C'CCAG_C Cuts at: 5144 5640 5144 Size: 496 5445 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 1163 Size: 573 621 4747 Fragments arranged by size: 4747 621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2278 2736 4002 4087 5158 2278 Size: 458 1266 85 1071 3061 Fragments arranged by size: 3061 1266 1071 458 85 BsmI GAATG_Cn' Cuts at: 2845 3452 3545 2845 Size: 607 93 5241 Fragments arranged by size: 5241 607 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2681 2681 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2092 2278 2736 3260 4002 4087 5158 Size: 399 302 186 458 524 742 85 1071 Cuts at: 5158 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 524 458 399 302 186 85 BspEI T'CCGG_A Cuts at: 3234 5604 3234 Size: 2370 3571 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2292 3204 3296 844 Size: 1448 912 92 3489 Fragments arranged by size: 3489 1448 912 92 BsrBI CCG'CTC Cuts at: 1394 1823 2031 2053 2094 2244 3828 1394 Size: 429 208 22 41 150 1584 3507 Fragments arranged by size: 3507 1584 429 208 150 41 22 BsrDI GCAATG_nn' Cuts at: 65 1248 2752 4436 4610 65 Size: 1183 1504 1684 174 1396 Fragments arranged by size: 1684 1504 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4585 4772 1700 Size: 110 2775 187 2869 Fragments arranged by size: 2869 2775 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 3169 794 Size: 2375 3566 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 2500 1184 Size: 1316 4625 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3215 2011 Size: 1204 4737 BstYI r'GATC_y Cuts at: 1972 2940 2974 3271 3638 4023 4040 5492 5578 Size: 968 34 297 367 385 17 1452 86 Cuts at: 5578 5590 5936 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 968 385 367 346 297 86 34 17 12 BtgI C'CryG_G Cuts at: 513 1065 2950 513 Size: 552 1885 3504 Fragments arranged by size: 3504 1885 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2893 487 Size: 683 56 1667 3535 Fragments arranged by size: 3535 1667 683 56 BtsI GCAGTG_nn' Cuts at: 3457 4251 4271 3457 Size: 794 20 5127 Fragments arranged by size: 5127 794 20 ClaI AT'CG_AT Cuts at: 1554 3213 1554 Size: 1659 4282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DraIII CAC_nnn'GTG Cuts at: 2122 2122 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2175 2239 Size: 54 1425 50 275 18 273 72 64 Cuts at: 2239 2613 2770 3052 3217 3388 4277 5906 8 Size: 374 157 282 165 171 889 1629 43 Fragments arranged by size: 1629 1425 889 374 282 275 273 171 165 157 72 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3871 1353 Size: 2518 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2198 2456 3194 4528 4902 5048 Size: 1064 138 609 258 738 1334 374 146 Cuts at: 5048 387 Size: 1280 Fragments arranged by size: 1334 1280 1064 738 609 374 258 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4004 4601 5388 5656 1239 Size: 393 2372 597 787 268 1524 Fragments arranged by size: 2372 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2069 2518 3028 3086 2069 Size: 449 510 58 4924 Fragments arranged by size: 4924 510 449 58 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2668 2668 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2143 2633 3078 5088 1678 Size: 465 490 445 2010 2531 Fragments arranged by size: 2531 2010 490 465 445 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2017 2049 2061 2093 4582 Size: 32 135 32 445 32 12 32 2489 Cuts at: 4582 4614 4656 4688 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2489 445 135 42 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 3229 748 Size: 2481 3460 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 3074 3074 Size: 5941 KpnI G_GTAC'C Cuts at: 2284 3266 2284 Size: 982 4959 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2615 2772 3054 10 Size: 54 1425 343 783 157 282 2897 Fragments arranged by size: 2897 1425 783 343 282 157 54 MslI CAynn'nnrTG Cuts at: 518 1166 2225 2379 2493 2739 2801 3213 3899 Size: 648 1059 154 114 246 62 412 686 Cuts at: 3899 4258 4417 518 Size: 359 159 2042 Fragments arranged by size: 2042 1059 686 648 412 359 246 159 154 114 62 MspA1I CmG'CkG Cuts at: 1606 2192 2334 2464 2476 3160 3357 4034 5182 Size: 586 142 130 12 684 197 677 1148 Cuts at: 5182 5427 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 684 677 586 245 197 142 130 12 NarI GG'CG_CC Cuts at: 3075 3075 Size: 5941 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5389 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NsiI A_TGCA'T Cuts at: 3069 3069 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 2611 3067 3299 4844 1132 Size: 97 1382 456 232 1545 2229 Fragments arranged by size: 2229 1545 1382 456 232 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PmlI CAC'GTG Cuts at: 2122 2377 2122 Size: 255 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2069 3028 3086 2069 Size: 959 58 4924 Fragments arranged by size: 4924 959 58 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2088 2088 Size: 5941 PstI C_TGCA'G Cuts at: 830 2289 3159 3165 3174 3293 830 Size: 1459 870 6 9 119 3478 Fragments arranged by size: 3478 1459 870 119 9 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2464 2476 3160 2464 Size: 12 684 5245 Fragments arranged by size: 5245 684 12 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 2069 2069 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3174 3293 3174 Size: 119 5822 ScaI AGT'ACT Cuts at: 2456 4189 5901 2456 Size: 1733 1712 2496 Fragments arranged by size: 2496 1733 1712 SfcI C'TryA_G Cuts at: 826 1045 2285 3155 3161 3170 3289 4424 5105 Size: 219 1240 870 6 9 119 1135 681 Cuts at: 5105 5296 826 Size: 191 1471 Fragments arranged by size: 1471 1240 1135 870 681 219 191 119 9 6 SfoI GGC'GCC Cuts at: 3076 3076 Size: 5941 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2038 3268 1872 Size: 166 1230 4545 Fragments arranged by size: 4545 1230 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 2834 4045 4946 5208 820 Size: 197 926 891 1211 901 262 1553 Fragments arranged by size: 1553 1211 926 901 891 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 3380 513 Size: 552 2315 3074 Fragments arranged by size: 3074 2315 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2695 3968 3985 4138 4323 4768 325 Size: 1255 1115 1273 17 153 185 445 1498 Fragments arranged by size: 1498 1273 1255 1115 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2454 2669 4187 Size: 274 80 33 51 1443 477 215 1518 Cuts at: 4187 5899 96 Size: 1712 138 Fragments arranged by size: 1712 1518 1443 477 274 215 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 5901 5933 2067 Size: 32 3802 32 2075 Fragments arranged by size: 3802 2075 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2654 2984 1316 Size: 1338 330 4273 Fragments arranged by size: 4273 1338 330 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2623 2623 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2036 3266 1870 Size: 166 1230 4545 Fragments arranged by size: 4545 1230 166 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2100 3190 3749 276 Size: 53 83 186 936 566 1090 559 2468 Fragments arranged by size: 2468 1090 936 566 559 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI