Restriction map for pFN21AB5307
 (Linear) (Six-base) MAPSORT of: KIBB5307.seq  Check: 1055  from: 1  to: 1236



KIBB5307.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AatII G_ACGT'C

Cuts at:      0      97    1187    1236 
   Size:         97    1090      49

  Fragments arranged by size: 

                1090      97      49
 
AccI GT'mk_AC

Cuts at:      0    1187    1236 
   Size:       1187      49
 
Acc65I G'GTAC_C

Cuts at:      0     275    1236 
   Size:        275     961
 
AfeI AGC'GCT

Cuts at:      0     136    1236 
   Size:        136    1100
 
AlwNI CAG_nnn'CTG

Cuts at:      0     405     474    1164    1236 
   Size:        405      69     690      72

  Fragments arranged by size: 

                 690     405      72      69
 
ApaI G_GGCC'C

Cuts at:      0      87    1236 
   Size:         87    1149
 
AvaI C'yCGr_G

Cuts at:      0      31     128    1236 
   Size:         31      97    1108

  Fragments arranged by size: 

                1108      97      31
 
BanI G'GyrC_C

Cuts at:      0     275    1069    1236 
   Size:        275     794     167

  Fragments arranged by size: 

                 794     275     167
 
BanII G_rGCy'C

Cuts at:      0      87    1236 
   Size:         87    1149
 
BbeI G_GCGC'C

Cuts at:      0    1073    1236 
   Size:       1073     163
 
BbvCI CC'TCA_GC

Cuts at:      0      19    1007    1236 
   Size:         19     988     229

  Fragments arranged by size: 

                 988     229      19
 
BclI T'GATC_A

Cuts at:      0     926    1236 
   Size:        926     310
 
BfrBI ATG'CAT

Cuts at:      0    1062    1236 
   Size:       1062     174
 
BglII A'GATC_T

Cuts at:      0     969    1236 
   Size:        969     267
 
Bme1580I G_kGCm'C

Cuts at:      0      87    1236 
   Size:         87    1149
 
BmgBI CAC'GTC

Cuts at:      0    1115    1236 
   Size:       1115     121
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     441    1068    1236 
   Size:        441     627     168

  Fragments arranged by size: 

                 627     441     168
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     213     323    1236 
   Size:        213     110     913

  Fragments arranged by size: 

                 913     213     110
 
Bpu10I CC'TnA_GC

Cuts at:      0      19    1007    1236 
   Size:         19     988     229

  Fragments arranged by size: 

                 988     229      19
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     814    1236 
   Size:        814     422
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1151    1236 
   Size:       1151      85
 
BsaAI yAC'GTr

Cuts at:      0     117     372     520    1236 
   Size:        117     255     148     716

  Fragments arranged by size: 

                 716     255     148     117
 
BsaBI GATnn'nnATC

Cuts at:      0     920    1236 
   Size:        920     316
 
BsaHI Gr'CG_yC

Cuts at:      0      10      94     717    1070    1184    1236 
   Size:         10      84     623     353     114      52

  Fragments arranged by size: 

                 623     353     114      84      52      10
 
BsaWI w'CCGG_w

Cuts at:      0    1229    1236 
   Size:       1229       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      62      92    1236 
   Size:         62      30    1144

  Fragments arranged by size: 

                1144      62      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      36    1236 
   Size:         36    1200
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    1236 
   Size:        352     884
 
BsiHKAI G_wGCw'C

Cuts at:      0     273     731    1236 
   Size:        273     458     505

  Fragments arranged by size: 

                 505     458     273
 
BsmI GAATG_Cn'

Cuts at:      0     840    1236 
   Size:        840     396
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     676    1236 
   Size:        676     560
 
Bsp1286I G_dGCh'C

Cuts at:      0      87     273     731    1236 
   Size:         87     186     458     505

  Fragments arranged by size: 

                 505     458     186      87
 
BspEI T'CCGG_A

Cuts at:      0    1229    1236 
   Size:       1229       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     287    1199    1236 
   Size:        287     912      37

  Fragments arranged by size: 

                 912     287      37
 
BsrBI CCG'CTC

Cuts at:      0      26      48      89     239    1236 
   Size:         26      22      41     150     997

  Fragments arranged by size: 

                 997     150      41      26      22
 
BsrDI GCAATG_nn'

Cuts at:      0     747    1236 
   Size:        747     489
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1164    1236 
   Size:       1164      72
 
BstEII G'GTnAC_C

Cuts at:      0     495    1236 
   Size:        495     741
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1210    1236 
   Size:       1210      26
 
BstYI r'GATC_y

Cuts at:      0     935     969    1236 
   Size:        935      34     267

  Fragments arranged by size: 

                 935     267      34
 
BtgI C'CryG_G

Cuts at:      0     945    1236 
   Size:        945     291
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     888    1236 
   Size:        888     348
 
ClaI AT'CG_AT

Cuts at:      0    1208    1236 
   Size:       1208      28
 
DraIII CAC_nnn'GTG

Cuts at:      0     117    1236 
   Size:        117    1119
 
EaeI y'GGCC_r

Cuts at:      0      98     170     234     608     765    1047    1212    1236 
   Size:         98      72      64     374     157     282     165      24

  Fragments arranged by size: 

                 374     282     165     157      98      72      64      24
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     193     451    1189    1236 
   Size:        193     258     738      47

  Fragments arranged by size: 

                 738     258     193      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0      64     513    1023    1081    1236 
   Size:         64     449     510      58     155

  Fragments arranged by size: 

                 510     449     155      64      58
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     663    1236 
   Size:        663     573
 
HaeII r_GCGC'y

Cuts at:      0     138     628    1073    1236 
   Size:        138     490     445     163

  Fragments arranged by size: 

                 490     445     163     138
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      12      44      56      88    1236 
   Size:         12      32      12      32    1148

  Fragments arranged by size: 

                1148      32      32      12      12
 
HindIII A'AGCT_T

Cuts at:      0    1224    1236 
   Size:       1224      12
 
KasI G'GCGC_C

Cuts at:      0    1069    1236 
   Size:       1069     167
 
KpnI G_GTAC'C

Cuts at:      0     279    1236 
   Size:        279     957
 
MscI TGG'CCA

Cuts at:      0     610     767    1049    1236 
   Size:        610     157     282     187

  Fragments arranged by size: 

                 610     282     187     157
 
MslI CAynn'nnrTG

Cuts at:      0     220     374     488     734     796    1208    1236 
   Size:        220     154     114     246      62     412      28

  Fragments arranged by size: 

                 412     246     220     154     114      62      28
 
MspA1I CmG'CkG

Cuts at:      0     187     329     459     471    1155    1236 
   Size:        187     142     130      12     684      81

  Fragments arranged by size: 

                 684     187     142     130      81      12
 
NarI GG'CG_CC

Cuts at:      0    1070    1236 
   Size:       1070     166
 
NsiI A_TGCA'T

Cuts at:      0    1064    1236 
   Size:       1064     172
 
NspI r_CATG'y

Cuts at:      0     606    1062    1236 
   Size:        606     456     174

  Fragments arranged by size: 

                 606     456     174
 
PmlI CAC'GTG

Cuts at:      0     117     372    1236 
   Size:        117     255     864

  Fragments arranged by size: 

                 864     255     117
 
PpuMI rG'GwC_Cy

Cuts at:      0      64    1023    1081    1236 
   Size:         64     959      58     155

  Fragments arranged by size: 

                 959     155      64      58
 
PspOMI G'GGCC_C

Cuts at:      0      83    1236 
   Size:         83    1153
 
PstI C_TGCA'G

Cuts at:      0     284    1154    1160    1169    1236 
   Size:        284     870       6       9      67

  Fragments arranged by size: 

                 870     284      67       9       6
 
PvuII CAG'CTG

Cuts at:      0     459     471    1155    1236 
   Size:        459      12     684      81

  Fragments arranged by size: 

                 684     459      81      12
 
SanDI GG'GwC_CC

Cuts at:      0      64    1236 
   Size:         64    1172
 
SbfI CC_TGCA'GG

Cuts at:      0    1169    1236 
   Size:       1169      67
 
ScaI AGT'ACT

Cuts at:      0     451    1236 
   Size:        451     785
 
SfcI C'TryA_G

Cuts at:      0     280    1150    1156    1165    1236 
   Size:        280     870       6       9      71

  Fragments arranged by size: 

                 870     280      71       9       6
 
SfoI GGC'GCC

Cuts at:      0    1071    1236 
   Size:       1071     165
 
SmaI CCC'GGG

Cuts at:      0      33    1236 
   Size:         33    1203
 
SmlI C'TyrA_G

Cuts at:      0     829    1236 
   Size:        829     407
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     690    1236 
   Size:        690     546
 
TatI w'GTAC_w

Cuts at:      0     449     664    1236 
   Size:        449     215     572

  Fragments arranged by size: 

                 572     449     215
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      62      94    1236 
   Size:         62      32    1142

  Fragments arranged by size: 

                1142      62      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     649     979    1236 
   Size:        649     330     257

  Fragments arranged by size: 

                 649     330     257
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     618    1236 
   Size:        618     618
 
XmaI C'CCGG_G

Cuts at:      0      31    1236 
   Size:         31    1205
 
ZraI GAC'GTC

Cuts at:      0      95    1185    1236 
   Size:         95    1090      51

  Fragments arranged by size: 

                1090      95      51
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AfeI    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI    BbvCI     BclI    BfrBI    BglII Bme1580I    BmgBI
     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI   BstAPI   BstEII    BstXI    BstYI
     BtgI    BtgZI     ClaI   DraIII     EaeI     EciI EcoO109I     FalI
    HaeII    Hin4I  HindIII     KasI     KpnI     MscI     MslI   MspA1I
     NarI     NsiI     NspI     PmlI    PpuMI   PspOMI     PstI    PvuII
    SanDI     SbfI     ScaI     SfcI     SfoI     SmaI     SmlI    TaqII
     TatI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AcuI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI     BcgI    BciVI     BglI     BlpI     BmtI
     BpmI    BseYI    BsiEI    BsiWI    BspHI    BsrFI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I   Bsu36I     BtsI    CspCI     DraI     DrdI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII     HpaI     MfeI     MluI     MmeI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     XbaI     XhoI     XmnI
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