(Circular) (Six-base) MAPSORT of: pFN21AB5294.seq Check: 5148 from: 1 to: 5734 pFN21AB5294 5734 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2482 2482 Size: 5734 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 3055 3055 Size: 5734 AclI AA'CG_TT Cuts at: 1563 2450 3861 4234 1563 Size: 887 1411 373 3063 Fragments arranged by size: 3063 1411 887 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 3797 5181 5449 1632 Size: 785 1380 1384 268 1917 Fragments arranged by size: 1917 1384 1380 785 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 2886 4633 5610 1203 Size: 1683 1747 977 1327 Fragments arranged by size: 1747 1683 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2450 2482 2450 Size: 32 5702 AlwNI CAG_nnn'CTG Cuts at: 3477 5049 3477 Size: 1572 4162 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 3043 3269 3421 5530 1493 Size: 1550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2389 3059 1870 Size: 73 446 670 4545 Fragments arranged by size: 4545 670 446 73 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3055 4510 618 Size: 325 217 627 1268 1455 1842 Fragments arranged by size: 1842 1455 1268 627 325 217 BanII G_rGCy'C Cuts at: 1391 2718 3053 1391 Size: 1327 335 4072 Fragments arranged by size: 4072 1327 335 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4763 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2710 2744 3925 3959 1903 Size: 34 50 34 689 34 1181 34 3678 Fragments arranged by size: 3678 1181 689 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2150 2612 Size: 107 122 71 631 605 337 141 462 Cuts at: 2612 4345 136 Size: 1733 1525 Fragments arranged by size: 1733 1525 631 605 462 337 141 122 107 71 BglII A'GATC_T Cuts at: 5729 5729 Size: 5734 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 2519 3795 4951 1790 Size: 729 1276 1156 2573 Fragments arranged by size: 2573 1276 1156 729 BmgBI CAC'GTC Cuts at: 1318 2889 1318 Size: 1571 4163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3465 4423 442 Size: 416 53 83 399 24 2048 958 1753 Fragments arranged by size: 2048 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 2674 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2008 2498 3024 4394 1239 Size: 769 490 526 1370 2579 Fragments arranged by size: 2579 1370 769 526 490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3859 4724 5022 3859 Size: 865 298 4571 Fragments arranged by size: 4571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2474 2852 4397 882 Size: 617 975 378 1545 2219 Fragments arranged by size: 2219 1545 975 617 378 BsaAI yAC'GTr Cuts at: 493 1204 3525 493 Size: 711 2321 2702 Fragments arranged by size: 2702 2321 711 BsaBI GATnn'nnATC Cuts at: 3026 5728 3026 Size: 2702 3032 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2089 3541 3923 4558 Size: 53 83 186 936 556 1452 382 635 Cuts at: 4558 275 Size: 1451 Fragments arranged by size: 1452 1451 936 635 556 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4167 4565 4839 4986 5397 4167 Size: 398 274 147 411 4504 Fragments arranged by size: 4504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2450 2480 1178 Size: 30 165 30 1047 30 4432 Fragments arranged by size: 4432 1047 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2804 1186 Size: 1618 4116 BseYI C'CCAG_C Cuts at: 2593 4937 5433 2593 Size: 2344 496 2894 Fragments arranged by size: 2894 2344 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5161 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3184 3945 4973 1540 Size: 275 186 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3795 3880 4951 3795 Size: 85 1071 4578 Fragments arranged by size: 4578 1071 85 BsmI GAATG_Cn' Cuts at: 2230 2760 2985 3245 3338 2230 Size: 530 225 260 93 4626 Fragments arranged by size: 4626 530 260 225 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2519 2718 3053 3795 3880 4951 1391 Size: 399 729 199 335 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 729 399 335 199 85 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 2988 3623 5353 2988 Size: 635 1730 3369 Fragments arranged by size: 3369 1730 635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2482 3089 844 Size: 1638 607 3489 Fragments arranged by size: 3489 1638 607 BsrBI CCG'CTC Cuts at: 1394 1823 2388 2619 3621 1394 Size: 429 565 231 1002 3507 Fragments arranged by size: 3507 1002 565 429 231 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4403 65 Size: 1183 2981 174 1396 Fragments arranged by size: 2981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2158 2438 4378 4565 1700 Size: 110 348 280 1940 187 2869 Fragments arranged by size: 2869 1940 348 280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5734 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5734 BstBI TT'CG_AA Cuts at: 4538 4538 Size: 5734 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstXI CCAn_nnnn'nTGG Cuts at: 3002 3002 Size: 5734 BstYI r'GATC_y Cuts at: 1972 3064 3431 3816 3833 5285 5371 5383 5729 Size: 1092 367 385 17 1452 86 12 346 Cuts at: 5729 1972 Size: 1977 Fragments arranged by size: 1977 1452 1092 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2799 2799 Size: 5734 BtgI C'CryG_G Cuts at: 513 1065 2955 513 Size: 552 1890 3292 Fragments arranged by size: 3292 1890 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2713 2755 487 Size: 683 56 1487 42 3466 Fragments arranged by size: 3466 1487 683 56 42 BtsI GCAGTG_nn' Cuts at: 2878 3250 4044 4064 2878 Size: 372 794 20 4548 Fragments arranged by size: 4548 794 372 20 ClaI AT'CG_AT Cuts at: 1554 2901 1554 Size: 1347 4387 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2128 2217 Size: 54 1425 50 275 18 180 118 89 Cuts at: 2217 2604 3181 4070 5699 8 Size: 387 577 889 1629 43 Fragments arranged by size: 1629 1425 889 577 387 275 180 118 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 3664 1353 Size: 999 1312 3423 Fragments arranged by size: 3423 1312 999 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2168 4321 4695 4841 387 Size: 1064 138 579 2153 374 146 1280 Fragments arranged by size: 2153 1280 1064 579 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2008 2417 2498 3024 3797 4394 5181 Size: 393 376 409 81 526 773 597 787 Cuts at: 5181 5449 1239 Size: 268 1524 Fragments arranged by size: 1524 787 773 597 526 409 393 376 268 81 EcoNI CCTnn'n_nnAGG Cuts at: 2409 2409 Size: 5734 EcoO109I rG'GnC_Cy Cuts at: 2401 2422 2401 Size: 21 5713 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2261 2525 2848 2261 Size: 264 323 5147 Fragments arranged by size: 5147 323 264 FspI TGC'GCA Cuts at: 2810 4240 2810 Size: 1430 4304 HaeII r_GCGC'y Cuts at: 1678 4881 1678 Size: 3203 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2116 2148 4375 4407 4449 Size: 32 135 32 544 32 2227 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 2227 544 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2431 3078 3324 669 Size: 988 281 493 647 246 3079 Fragments arranged by size: 3079 988 647 493 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 2431 3324 2431 Size: 893 4841 KpnI G_GTAC'C Cuts at: 3059 3059 Size: 5734 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 5610 5610 Size: 5734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2114 3039 4848 5032 1305 Size: 809 925 1809 184 2007 Fragments arranged by size: 2007 1809 925 809 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2012 2219 2606 10 Size: 54 1425 343 180 207 387 3138 Fragments arranged by size: 3138 1425 387 343 207 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 3692 4051 4210 518 Size: 648 2526 359 159 2042 Fragments arranged by size: 2526 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3150 3827 4975 5220 1606 Size: 1544 677 1148 245 2120 Fragments arranged by size: 2120 1544 1148 677 245 NaeI GCC'GGC Cuts at: 2440 2440 Size: 5734 NcoI C'CATG_G Cuts at: 513 1065 2955 513 Size: 552 1890 3292 Fragments arranged by size: 3292 1890 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NgoMIV G'CCGG_C Cuts at: 2438 2438 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 2419 2593 3092 4637 1132 Size: 97 1190 174 499 1545 2229 Fragments arranged by size: 2229 1545 1190 499 174 97 PasI CC'CwG_GG Cuts at: 1348 2689 1348 Size: 1341 4393 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 2956 1108 Size: 1848 3886 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2450 2482 3803 3835 5316 5348 2450 Size: 32 1321 32 1481 32 2836 Fragments arranged by size: 2836 1481 1321 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2401 2401 Size: 5734 PshAI GACnn'nnGTC Cuts at: 1613 2032 1613 Size: 419 5315 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PspXI vC'TCGA_Gb Cuts at: 2389 2389 Size: 5734 PstI C_TGCA'G Cuts at: 830 2351 2870 3086 830 Size: 1521 519 216 3478 Fragments arranged by size: 3478 1521 519 216 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 2831 3982 5694 2831 Size: 1151 1712 2871 Fragments arranged by size: 2871 1712 1151 SfcI C'TryA_G Cuts at: 826 1045 2347 2866 3082 4217 4898 5089 826 Size: 219 1302 519 216 1135 681 191 1471 Fragments arranged by size: 1471 1302 1135 681 519 219 216 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2612 2612 Size: 5734 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 3061 1872 Size: 1189 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2389 3838 4739 5001 820 Size: 197 926 446 1449 901 262 1553 Fragments arranged by size: 1553 1449 926 901 446 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 2593 3092 1132 Size: 1461 499 3774 Fragments arranged by size: 3774 1461 499 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 2955 3173 513 Size: 552 1890 218 3074 Fragments arranged by size: 3074 1890 552 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2334 3761 3778 3931 4116 4561 325 Size: 1255 754 1427 17 153 185 445 1498 Fragments arranged by size: 1498 1427 1255 754 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2829 3980 5692 Size: 274 80 33 51 1443 852 1151 1712 Cuts at: 5692 96 Size: 138 Fragments arranged by size: 1712 1443 1151 852 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5694 5726 5694 Size: 32 5702 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XhoI C'TCGA_G Cuts at: 1943 2389 1943 Size: 446 5288 XmaI C'CCGG_G Cuts at: 1870 3059 1870 Size: 1189 4545 XmnI GAAnn'nnTTC Cuts at: 3863 3863 Size: 5734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI