(Circular) (Six-base) MAPSORT of: pFN21AE0668.seq Check: 1429 from: 1 to: 5884 pFN21AE0668 5884 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3694 278 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3227 1937 Size: 1290 4594 Acc65I G'GTAC_C Cuts at: 3205 3205 Size: 5884 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 2489 3947 5331 5599 1632 Size: 581 276 1458 1384 268 1917 Fragments arranged by size: 1917 1458 1384 581 276 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 2691 4783 5760 1203 Size: 1488 2092 977 1327 Fragments arranged by size: 2092 1488 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 4613 4613 Size: 5884 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5884 AlwNI CAG_nnn'CTG Cuts at: 2356 2427 3627 5199 2356 Size: 71 1200 1572 3041 Fragments arranged by size: 3041 1572 1200 71 ApaLI G'TGCA_C Cuts at: 3941 5097 3941 Size: 1156 4728 ApoI r'AATT_y Cuts at: 1493 2484 2496 3002 3193 3419 3571 5680 1493 Size: 991 12 506 191 226 152 2109 1697 Fragments arranged by size: 2109 1697 991 506 226 191 152 12 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 2882 3209 1870 Size: 73 939 327 4545 Fragments arranged by size: 4545 939 327 73 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2832 3205 4660 618 Size: 325 217 627 1045 373 1455 1842 Fragments arranged by size: 1842 1455 1045 627 373 325 217 BanII G_rGCy'C Cuts at: 1391 3203 1391 Size: 1812 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4913 BbvCI CC'TCA_GC Cuts at: 3102 3102 Size: 5884 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2039 2073 4075 4109 1903 Size: 34 50 34 18 34 2002 34 3678 Fragments arranged by size: 3678 2002 50 34 34 34 34 18 BciVI GTATCCnnnnn_n' Cuts at: 2796 3776 4986 2796 Size: 980 1210 3694 Fragments arranged by size: 3694 1210 980 BclI T'GATC_A Cuts at: 1548 1548 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4495 136 Size: 107 122 71 631 605 2823 1525 Fragments arranged by size: 2823 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3092 5879 3092 Size: 2787 3097 BlpI GC'TnA_GC Cuts at: 3301 3301 Size: 5884 Bme1580I G_kGCm'C Cuts at: 1790 2681 3945 5101 1790 Size: 891 1264 1156 2573 Fragments arranged by size: 2573 1264 1156 891 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5884 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2976 3615 4573 Size: 416 53 83 399 24 1559 639 958 Cuts at: 4573 442 Size: 1753 Fragments arranged by size: 1753 1559 958 639 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2386 2386 Size: 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2327 2693 2764 2784 2837 4544 1239 Size: 896 192 366 71 20 53 1707 2579 Fragments arranged by size: 2579 1707 896 366 192 71 53 20 Bpu10I CC'TnA_GC Cuts at: 2894 3102 2894 Size: 208 5676 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 4009 4874 5172 2174 Size: 1835 865 298 2886 Fragments arranged by size: 2886 1835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2078 2263 2852 4547 882 Size: 617 579 185 589 1695 2219 Fragments arranged by size: 2219 1695 617 589 579 185 BsaAI yAC'GTr Cuts at: 493 1204 3675 493 Size: 711 2471 2702 Fragments arranged by size: 2702 2471 711 BsaBI GATnn'nnATC Cuts at: 2423 5878 2423 Size: 3455 2429 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2024 3180 3691 4073 Size: 53 83 186 936 491 1156 511 382 Cuts at: 4073 4708 275 Size: 635 1451 Fragments arranged by size: 1451 1156 936 635 511 491 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2325 4317 4715 4989 5136 5547 2325 Size: 1992 398 274 147 411 2662 Fragments arranged by size: 2662 1992 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2810 2840 1178 Size: 30 165 30 1407 30 4222 Fragments arranged by size: 4222 1407 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2787 2958 1186 Size: 946 655 171 4112 Fragments arranged by size: 4112 946 655 171 BseYI C'CCAG_C Cuts at: 2224 3155 5087 5583 2224 Size: 931 1932 496 2525 Fragments arranged by size: 2525 1932 931 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2750 1163 Size: 573 1014 4297 Fragments arranged by size: 4297 1014 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3334 4095 5123 1540 Size: 275 186 1333 761 1028 2301 Fragments arranged by size: 2301 1333 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2439 3945 4030 5101 2439 Size: 1506 85 1071 3222 Fragments arranged by size: 3222 1506 1071 85 BsmI GAATG_Cn' Cuts at: 3395 3488 3395 Size: 93 5791 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2439 2681 3203 3945 4030 5101 1391 Size: 399 649 242 522 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 649 522 399 242 85 BspEI T'CCGG_A Cuts at: 2325 5547 2325 Size: 3222 2662 BspHI T'CATG_A Cuts at: 3773 5503 3773 Size: 1730 4154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2341 3110 3239 844 Size: 1497 769 129 3489 Fragments arranged by size: 3489 1497 769 129 BsrBI CCG'CTC Cuts at: 1394 1823 3771 1394 Size: 429 1948 3507 Fragments arranged by size: 3507 1948 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2242 2514 4379 4553 65 Size: 1183 994 272 1865 174 1396 Fragments arranged by size: 1865 1396 1183 994 272 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4528 4715 1700 Size: 110 2718 187 2869 Fragments arranged by size: 2869 2718 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5884 BssSI C'ACGA_G Cuts at: 3944 4956 3944 Size: 1012 4872 BstAPI GCAn_nnn'nTGC Cuts at: 794 2356 794 Size: 1562 4322 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5884 BstXI CCAn_nnnn'nTGG Cuts at: 2815 3176 2815 Size: 361 5523 BstYI r'GATC_y Cuts at: 1972 3092 3214 3581 3966 3983 5435 5521 5533 Size: 1120 122 367 385 17 1452 86 12 Cuts at: 5533 5879 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1120 385 367 346 122 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2605 2658 2925 513 Size: 552 1540 53 267 3472 Fragments arranged by size: 3472 1540 552 267 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2424 487 Size: 683 56 1198 3947 Fragments arranged by size: 3947 1198 683 56 BtsI GCAGTG_nn' Cuts at: 3400 4194 4214 3400 Size: 794 20 5070 Fragments arranged by size: 5070 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2587 2622 529 Size: 35 2023 35 3791 Fragments arranged by size: 3791 2023 35 35 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2633 4891 809 Size: 507 1317 2258 1802 Fragments arranged by size: 2258 1802 1317 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2286 2661 3331 Size: 54 1425 50 275 18 456 375 670 Cuts at: 3331 4220 5849 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 670 456 375 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3331 1537 Size: 275 1519 4090 Fragments arranged by size: 4090 1519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3814 1353 Size: 2461 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4471 4845 4991 387 Size: 1064 138 2882 374 146 1280 Fragments arranged by size: 2882 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2213 2327 2489 2693 2764 2784 Size: 393 503 78 114 162 204 71 20 Cuts at: 2784 2837 3947 4544 5331 5599 1239 Size: 53 1110 597 787 268 1524 Fragments arranged by size: 1524 1110 787 597 503 393 268 204 162 114 78 71 53 20 EcoO109I rG'GnC_Cy Cuts at: 2685 2877 2889 2685 Size: 192 12 5680 Fragments arranged by size: 5680 192 12 EcoRI G'AATT_C Cuts at: 2484 3193 3571 2484 Size: 709 378 4797 Fragments arranged by size: 4797 709 378 FspI TGC'GCA Cuts at: 4390 4390 Size: 5884 HaeII r_GCGC'y Cuts at: 1678 2152 5031 1678 Size: 474 2879 2531 Fragments arranged by size: 2879 2531 474 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4525 4557 4599 4631 1373 Size: 32 135 32 2953 32 42 32 2626 Fragments arranged by size: 2953 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2065 2599 2959 3228 3474 669 Size: 988 281 127 534 360 269 246 3079 Fragments arranged by size: 3079 988 534 360 281 269 246 127 HindIII A'AGCT_T Cuts at: 748 748 Size: 5884 HpaI GTT'AAC Cuts at: 2065 3474 2065 Size: 1409 4475 KpnI G_GTAC'C Cuts at: 3209 3209 Size: 5884 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2610 2925 4998 5182 1305 Size: 1305 315 2073 184 2007 Fragments arranged by size: 2073 2007 1305 315 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2288 2663 10 Size: 54 1425 343 456 375 3231 Fragments arranged by size: 3231 1425 456 375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2442 2571 3842 4201 4360 518 Size: 648 1276 129 1271 359 159 2042 Fragments arranged by size: 2042 1276 1271 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 2035 2640 3300 3977 5125 5370 1606 Size: 429 605 660 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 660 605 429 245 NcoI C'CATG_G Cuts at: 513 1065 2658 2925 513 Size: 552 1593 267 3472 Fragments arranged by size: 3472 1593 552 267 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 2695 3242 4787 1132 Size: 97 1466 547 1545 2229 Fragments arranged by size: 2229 1545 1466 547 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5884 PciI A'CATG_T Cuts at: 2691 4783 2691 Size: 2092 3792 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5884 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3953 3985 5466 5498 3953 Size: 32 1481 32 4339 Fragments arranged by size: 4339 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2685 2877 2685 Size: 192 5692 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5884 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PstI C_TGCA'G Cuts at: 830 2062 2352 3236 830 Size: 1232 290 884 3478 Fragments arranged by size: 3478 1232 884 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 PvuII CAG'CTG Cuts at: 2035 2035 Size: 5884 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SfcI C'TryA_G Cuts at: 826 1045 2058 2348 2404 3232 4367 5048 5239 Size: 219 1013 290 56 828 1135 681 191 Cuts at: 5239 826 Size: 1471 Fragments arranged by size: 1471 1135 1013 828 681 290 219 191 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 2884 3211 1872 Size: 1012 327 4545 Fragments arranged by size: 4545 1012 327 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 3988 4889 5151 820 Size: 197 926 210 1835 901 262 1553 Fragments arranged by size: 1835 1553 926 901 262 210 197 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 3242 1132 Size: 2110 3774 SspI AAT'ATT Cuts at: 5 52 3808 5 Size: 47 3756 2081 Fragments arranged by size: 3756 2081 47 StuI AGG'CCT Cuts at: 1517 2099 2159 2532 1517 Size: 582 60 373 4869 Fragments arranged by size: 4869 582 373 60 StyI C'CwwG_G Cuts at: 513 1065 2658 2808 2873 2925 3323 513 Size: 552 1593 150 65 52 398 3074 Fragments arranged by size: 3074 1593 552 398 150 65 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2820 2881 3911 3928 4081 4266 4711 Size: 1255 1240 61 1030 17 153 185 445 Cuts at: 4711 325 Size: 1498 Fragments arranged by size: 1498 1255 1240 1030 445 185 153 61 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4130 5842 96 Size: 274 80 33 51 1443 2153 1712 138 Fragments arranged by size: 2153 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5844 5876 5844 Size: 32 5852 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5884 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2270 2671 2270 Size: 401 5483 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5884 XmaI C'CCGG_G Cuts at: 1870 2882 3209 1870 Size: 1012 327 4545 Fragments arranged by size: 4545 1012 327 XmnI GAAnn'nnTTC Cuts at: 4013 4013 Size: 5884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3692 276 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI