(Circular) (Six-base) MAPSORT of: pFN21AB8730.seq Check: 2496 from: 1 to: 5884 pFN21AB8730 5884 bp With 182 enzymes: SgfI * May 6, 2010 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3694 278 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 3227 1937 Size: 1194 96 4594 Fragments arranged by size: 4594 1194 96 Acc65I G'GTAC_C Cuts at: 3205 3205 Size: 5884 AclI AA'CG_TT Cuts at: 1563 2094 4011 4384 1563 Size: 531 1917 373 3063 Fragments arranged by size: 3063 1917 531 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2190 2978 3131 3947 5331 5599 1632 Size: 558 788 153 816 1384 268 1917 Fragments arranged by size: 1917 1384 816 788 558 268 153 AfeI AGC'GCT Cuts at: 2546 3014 2546 Size: 468 5416 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 2712 4783 5760 1203 Size: 1509 2071 977 1327 Fragments arranged by size: 2071 1509 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 4613 4613 Size: 5884 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5884 AlwNI CAG_nnn'CTG Cuts at: 2210 2727 3627 5199 2210 Size: 517 900 1572 2895 Fragments arranged by size: 2895 1572 900 517 ApaLI G'TGCA_C Cuts at: 3941 5097 3941 Size: 1156 4728 ApoI r'AATT_y Cuts at: 1493 2602 3193 3419 3571 5680 1493 Size: 1109 591 226 152 2109 1697 Fragments arranged by size: 2109 1697 1109 591 226 152 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 3209 1870 Size: 73 1266 4545 Fragments arranged by size: 4545 1266 73 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2465 2656 3205 4660 618 Size: 325 217 627 678 191 549 1455 1842 Fragments arranged by size: 1842 1455 678 627 549 325 217 191 BanII G_rGCy'C Cuts at: 1391 2040 3097 3203 1391 Size: 649 1057 106 4072 Fragments arranged by size: 4072 1057 649 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 2784 928 Size: 971 279 606 4028 Fragments arranged by size: 4028 971 606 279 BbvCI CC'TCA_GC Cuts at: 2577 2577 Size: 5884 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4075 4109 1903 Size: 34 2138 34 3678 Fragments arranged by size: 3678 2138 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3776 4986 3776 Size: 1210 4674 BclI T'GATC_A Cuts at: 1548 1548 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4495 136 Size: 107 122 71 631 605 2823 1525 Fragments arranged by size: 2823 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5879 5879 Size: 5884 BlpI GC'TnA_GC Cuts at: 3301 3301 Size: 5884 Bme1580I G_kGCm'C Cuts at: 1790 2661 3945 5101 1790 Size: 871 1284 1156 2573 Fragments arranged by size: 2573 1284 1156 871 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5884 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2807 3615 4573 Size: 416 53 83 399 24 1390 808 958 Cuts at: 4573 442 Size: 1753 Fragments arranged by size: 1753 1390 958 808 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2566 2698 3111 2566 Size: 132 413 5339 Fragments arranged by size: 5339 413 132 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2767 3085 4544 1239 Size: 1528 318 1459 2579 Fragments arranged by size: 2579 1528 1459 318 Bpu10I CC'TnA_GC Cuts at: 2577 2577 Size: 5884 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3038 4009 4874 5172 3038 Size: 971 865 298 3750 Fragments arranged by size: 3750 971 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2838 4547 882 Size: 617 1339 1709 2219 Fragments arranged by size: 2219 1709 1339 617 BsaAI yAC'GTr Cuts at: 493 1204 2379 3675 493 Size: 711 1175 1296 2702 Fragments arranged by size: 2702 1296 1175 711 BsaBI GATnn'nnATC Cuts at: 5878 5878 Size: 5884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3691 4073 4708 275 Size: 53 83 186 936 2158 382 635 1451 Fragments arranged by size: 2158 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2745 4317 4715 4989 5136 5547 2745 Size: 1572 398 274 147 411 3082 Fragments arranged by size: 3082 1572 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 2927 2957 1178 Size: 30 165 30 600 30 894 30 4105 Fragments arranged by size: 4105 894 600 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2565 2935 1186 Size: 838 541 370 4135 Fragments arranged by size: 4135 838 541 370 BseYI C'CCAG_C Cuts at: 2952 3068 5087 5583 2952 Size: 116 2019 496 3253 Fragments arranged by size: 3253 2019 496 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2214 2244 2453 1163 Size: 573 478 30 209 4594 Fragments arranged by size: 4594 573 478 209 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3334 4095 5123 1540 Size: 275 186 1333 761 1028 2301 Fragments arranged by size: 2301 1333 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2915 2926 3945 4030 5101 2915 Size: 11 1019 85 1071 3698 Fragments arranged by size: 3698 1071 1019 85 11 BsmI GAATG_Cn' Cuts at: 2205 2347 3395 3488 2205 Size: 142 1048 93 4601 Fragments arranged by size: 4601 1048 142 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2661 2915 2926 3097 3203 3945 Size: 399 250 621 254 11 171 106 742 Cuts at: 3945 4030 5101 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 621 399 254 250 171 106 85 11 BspEI T'CCGG_A Cuts at: 5547 5547 Size: 5884 BspHI T'CATG_A Cuts at: 3773 5503 3773 Size: 1730 4154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2432 3239 844 Size: 1588 807 3489 Fragments arranged by size: 3489 1588 807 BsrBI CCG'CTC Cuts at: 1394 1823 3771 1394 Size: 429 1948 3507 Fragments arranged by size: 3507 1948 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 4379 4553 65 Size: 1183 879 2252 174 1396 Fragments arranged by size: 2252 1396 1183 879 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2248 2847 4528 4715 1700 Size: 110 438 599 1681 187 2869 Fragments arranged by size: 2869 1681 599 438 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5884 BssSI C'ACGA_G Cuts at: 2894 3944 4956 2894 Size: 1050 1012 3822 Fragments arranged by size: 3822 1050 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5884 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5884 BstYI r'GATC_y Cuts at: 1972 2023 2997 3214 3581 3966 3983 5435 5521 Size: 51 974 217 367 385 17 1452 86 Cuts at: 5521 5533 5879 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 974 385 367 346 217 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 2673 2673 Size: 5884 BtgI C'CryG_G Cuts at: 513 1065 2004 2481 3001 513 Size: 552 939 477 520 3396 Fragments arranged by size: 3396 939 552 520 477 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2574 487 Size: 683 56 1348 3797 Fragments arranged by size: 3797 1348 683 56 BtsI GCAGTG_nn' Cuts at: 2924 3400 4194 4214 2924 Size: 476 794 20 4594 Fragments arranged by size: 4594 794 476 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2362 4891 809 Size: 507 1046 2529 1802 Fragments arranged by size: 2529 1802 1046 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2415 2484 3331 Size: 54 1425 50 275 18 585 69 847 Cuts at: 3331 4220 5849 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 847 585 275 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3331 1537 Size: 275 1519 4090 Fragments arranged by size: 4090 1519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2856 3814 1353 Size: 1503 958 3423 Fragments arranged by size: 3423 1503 958 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4471 4845 4991 387 Size: 1064 138 2882 374 146 1280 Fragments arranged by size: 2882 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2190 2767 2978 3085 3131 3947 4544 Size: 393 558 577 211 107 46 816 597 Cuts at: 4544 5331 5599 1239 Size: 787 268 1524 Fragments arranged by size: 1524 816 787 597 577 558 393 268 211 107 46 EcoO109I rG'GnC_Cy Cuts at: 3170 3170 Size: 5884 EcoRI G'AATT_C Cuts at: 3193 3571 3193 Size: 378 5506 FspI TGC'GCA Cuts at: 4390 4390 Size: 5884 HaeII r_GCGC'y Cuts at: 1678 2530 2548 3016 5031 1678 Size: 852 18 468 2015 2531 Fragments arranged by size: 2531 2015 852 468 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 4525 4557 4599 Size: 32 135 32 431 32 2490 32 42 Cuts at: 4599 4631 1373 Size: 32 2626 Fragments arranged by size: 2626 2490 431 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3228 3474 669 Size: 988 281 1290 246 3079 Fragments arranged by size: 3079 1290 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5884 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KpnI G_GTAC'C Cuts at: 3209 3209 Size: 5884 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4998 5182 1305 Size: 3693 184 2007 Fragments arranged by size: 3693 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2417 2486 10 Size: 54 1425 343 585 69 3408 Fragments arranged by size: 3408 1425 585 343 69 54 MslI CAynn'nnrTG Cuts at: 518 1166 3842 4201 4360 518 Size: 648 2676 359 159 2042 Fragments arranged by size: 2676 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2437 3300 3977 5125 5370 1606 Size: 831 863 677 1148 245 2120 Fragments arranged by size: 2120 1148 863 831 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2481 513 Size: 552 939 477 3916 Fragments arranged by size: 3916 939 552 477 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 2716 3242 4787 1132 Size: 97 1487 526 1545 2229 Fragments arranged by size: 2229 1545 1487 526 97 PasI CC'CwG_GG Cuts at: 1348 2906 1348 Size: 1558 4326 PciI A'CATG_T Cuts at: 2712 4783 2712 Size: 2071 3813 PflMI CCAn_nnn'nTGG Cuts at: 1108 2122 2975 1108 Size: 1014 853 4017 Fragments arranged by size: 4017 1014 853 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3953 3985 5466 5498 3953 Size: 32 1481 32 4339 Fragments arranged by size: 4339 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2178 1613 Size: 565 5319 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PstI C_TGCA'G Cuts at: 830 3236 830 Size: 2406 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 PvuII CAG'CTG Cuts at: 2437 2437 Size: 5884 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SfcI C'TryA_G Cuts at: 826 1045 3232 4367 5048 5239 826 Size: 219 2187 1135 681 191 1471 Fragments arranged by size: 2187 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 2248 1700 Size: 548 5336 SmaI CCC'GGG Cuts at: 1872 3211 1872 Size: 1339 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3053 3988 4889 5151 820 Size: 197 926 1110 935 901 262 1553 Fragments arranged by size: 1553 1110 935 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 3242 1132 Size: 2110 3774 SspI AAT'ATT Cuts at: 5 52 2984 3808 5 Size: 47 2932 824 2081 Fragments arranged by size: 2932 2081 824 47 StuI AGG'CCT Cuts at: 1517 3044 1517 Size: 1527 4357 StyI C'CwwG_G Cuts at: 513 1065 2004 2481 3045 3323 513 Size: 552 939 477 564 278 3074 Fragments arranged by size: 3074 939 564 552 477 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3911 3928 4081 4266 4711 325 Size: 1255 2331 17 153 185 445 1498 Fragments arranged by size: 2331 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4130 5842 96 Size: 274 80 33 51 1443 2153 1712 138 Fragments arranged by size: 2153 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3098 3130 5844 5876 3098 Size: 32 2714 32 3106 Fragments arranged by size: 3106 2714 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5884 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2271 2770 2271 Size: 499 5385 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5884 XmaI C'CCGG_G Cuts at: 1870 3209 1870 Size: 1339 4545 XmnI GAAnn'nnTTC Cuts at: 4013 4013 Size: 5884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3692 276 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI