################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspI MunI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AccI 202 GTATAC GT^[AC][GT]AC AccII 674 CGCG CG^CG AflIII 979 ACATGT A^C[GA][CT]GT Alw44I 12 GTGCAC G^TGCAC ApaLI 12 GTGCAC G^TGCAC AspHI 12 GTGCAC G[AT]GC[AT]^C BamHI 3 GGATCC G^GATCC BbsI 951 GAAGAC GAAGAC(2/6) BbvCI 715 CCTCAGC CCTCAGC(-5/-2) BbvI 67 GCTGC GCAGC(8/12) BfaI 157 CTAG C^TAG BglII 916 AGATCT A^GATCT BmyI 12 GTGCAC G[AGT]GC[ACT]^C BpuAI 951 GAAGAC GAAGAC(2/6) BsaBI 360 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 739 CCTTGG C^C[ACGT][ACGT]GG BseRI 71 CTCCTC GAGGAG(10/8) BsiHKAI 12 GTGCAC G[AT]GC[AT]^C BsmFI 21 GGGAC GGGAC(10/14) Bsp1286I 12 GTGCAC G[AGT]GC[ACT]^C Bsp1407I 452 TGTACA T^GTACA BspLU11I 979 ACATGT A^CATGT BsrGI 452 TGTACA T^GTACA BsrI 9 CCAGT ACTGG(1/-1) BssKI 177 CCAGG ^CC[ACGT]GG Bst1107I 202 GTATAC GTA^TAC BstBI 948 TTCGAA TT^CGAA BstEII 484 GGTTACC G^GT[ACGT]ACC BstNI 177 CCAGG CC^[AT]GG BstPI 484 GGTTACC G^GT[ACGT]ACC BstUI 674 CGCG CG^CG BstZ17I 202 GTATAC GTA^TAC Cac8I 396 GCTTGC GC[ACGT]^[ACGT]GC DraI 940 TTTAAA TTT^AAA DraII 710 AGGACCC [GA]G^G[ACGT]CC[CT] EarI 868 GAAGAG CTCTTC(1/4) EcoO109I 710 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 484 GGTTACC G^GT[ACGT]ACC EcoRII 177 CCAGG ^CC[AT]GG EcoT14I 739 CCTTGG C^C[AT][AT]GG HgaI 672 GACGC GACGC(5/10) HincII 410 GTCAAC GT[CT]^[GA]AC HindII 410 GTCAAC GT[CT]^[GA]AC HindIII 86 AAGCTT A^AGCTT HphI 688 TCACC GGTGA(8/7) Ksp632I 868 GAAGAG CTCTTC(1/4) MaeI 157 CTAG C^TAG MaeII 593 ACGT A^CGT MamI 360 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 941 TTAA T^TAA MspA1I 749 CAGCTG C[AC]G^C[GT]G MvaI 177 CCAGG CC^[AT]GG MvnI 674 CGCG CG^CG NspI 979 ACATGT [GA]CATG^[CT] NspV 948 TTCGAA TT^CGAA PpuMI 710 AGGACCC [GA]G^G[AT]CC[CT] PstI 104 CTGCAG CTGCA^G PvuII 749 CAGCTG CAG^CTG ScrFI 177 CCAGG CC^[ACGT]GG SfaNI 365 GCATC GCATC(5/9) SfuI 948 TTCGAA TT^CGAA SspBI 452 TGTACA T^GTACA StyI 739 CCTTGG C^C[AT][AT]GG Tru9I 941 TTAA T^TAA TseI 67 GCTGC G^C[AT]GC Tsp45I 577 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwI 4 GATCC GGATC(4/5) 3 GGATC BsmAI 100 GAGAC GTCTC(1/5) 285 GAGAC BspMI 110 ACCTGC ACCTGC(4/8) 466 ACCTGC BstF5I 333 CATCC GGATG(2/0) 366 CATCC BstXI 239 CCAAGTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 851 CCAACACAATGG BstYI 3 GGATCC [GA]^GATC[CT] 916 AGATCT EcoRI 378 GAATTC G^AATTC 528 GAATTC FokI 333 CATCC GGATG(9/13) 366 CATCC MflI 3 GGATCC [GA]^GATC[CT] 916 AGATCT MslI 351 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 956 CATCTTCATG SfcI 104 CTGCAG C^T[GA][CT]AG 698 CTATAG SmlI 566 CTCAAG C^T[CT][GA]AG 706 CTTGAG TaqI 539 TCGA T^CGA 949 TCGA TthHB8I 539 TCGA T^CGA 949 TCGA XhoII 3 GGATCC [GA]^GATC[CT] 916 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII Cfr13I DdeI DpnII Fnu4HI HaeIII HinfI ItaI MaeIII MboI MboII MnlI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I TfiI Tsp509I TspRI