(Circular) (Six-base) MAPSORT of: pFN21AE1521.seq Check: 8664 from: 1 to: 5692 pFN21AE1521 5692 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2208 3035 1937 Size: 271 827 4594 Fragments arranged by size: 4594 827 271 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 2288 2395 3755 5139 5407 1632 Size: 470 186 107 1360 1384 268 1917 Fragments arranged by size: 1917 1384 1360 470 268 186 107 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 2984 4591 5568 1203 Size: 1781 1607 977 1327 Fragments arranged by size: 1781 1607 1327 977 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5692 AlwNI CAG_nnn'CTG Cuts at: 3435 5007 3435 Size: 1572 4120 ApaLI G'TGCA_C Cuts at: 2017 3749 4905 2017 Size: 1732 1156 2804 Fragments arranged by size: 2804 1732 1156 ApoI r'AATT_y Cuts at: 1493 2225 2383 2464 2533 3001 3227 3379 5488 Size: 732 158 81 69 468 226 152 2109 Cuts at: 5488 1493 Size: 1697 Fragments arranged by size: 2109 1697 732 468 226 158 152 81 69 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 3017 1870 Size: 73 1074 4545 Fragments arranged by size: 4545 1074 73 BamHI G'GATC_C Cuts at: 2008 3022 3389 2008 Size: 1014 367 4311 Fragments arranged by size: 4311 1014 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3013 4468 618 Size: 325 217 627 1226 1455 1842 Fragments arranged by size: 1842 1455 1226 627 325 217 BanII G_rGCy'C Cuts at: 1391 3011 1391 Size: 1620 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2963 928 Size: 971 1064 3657 Fragments arranged by size: 3657 1064 971 BbvCI CC'TCA_GC Cuts at: 2721 2721 Size: 5692 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3584 4794 3584 Size: 1210 4482 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4303 136 Size: 107 122 71 631 605 2631 1525 Fragments arranged by size: 2631 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2921 5687 2921 Size: 2766 2926 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 2021 3753 4909 1790 Size: 231 1732 1156 2573 Fragments arranged by size: 2573 1732 1156 231 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2008 3423 4381 Size: 416 53 83 399 24 591 1415 958 Cuts at: 4381 442 Size: 1753 Fragments arranged by size: 1753 1415 958 591 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4352 1239 Size: 3113 2579 Bpu10I CC'TnA_GC Cuts at: 2721 2721 Size: 5692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2556 2732 3817 4682 4980 2556 Size: 176 1085 865 298 3268 Fragments arranged by size: 3268 1085 865 298 176 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 882 Size: 617 2856 2219 Fragments arranged by size: 2856 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3483 493 Size: 711 2279 2702 Fragments arranged by size: 2702 2279 711 BsaBI GATnn'nnATC Cuts at: 2369 5686 2369 Size: 3317 2375 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3499 3881 4516 275 Size: 53 83 186 936 1966 382 635 1451 Fragments arranged by size: 1966 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4125 4523 4797 4944 5355 4125 Size: 398 274 147 411 4462 Fragments arranged by size: 4462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5467 Fragments arranged by size: 5467 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2067 1186 Size: 881 4811 BseYI C'CCAG_C Cuts at: 2934 4895 5391 2934 Size: 1961 496 3235 Fragments arranged by size: 3235 1961 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5119 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3142 3903 4931 1540 Size: 275 186 1141 761 1028 2301 Fragments arranged by size: 2301 1141 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2021 3753 3838 4909 2021 Size: 1732 85 1071 2804 Fragments arranged by size: 2804 1732 1071 85 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 3011 3753 3838 4909 1391 Size: 399 231 990 742 85 1071 2174 Fragments arranged by size: 2174 1071 990 742 399 231 85 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 2480 3047 844 Size: 1280 356 567 3489 Fragments arranged by size: 3489 1280 567 356 BsrBI CCG'CTC Cuts at: 1394 1823 3579 1394 Size: 429 1756 3507 Fragments arranged by size: 3507 1756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4187 4361 65 Size: 1183 2939 174 1396 Fragments arranged by size: 2939 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4336 4523 1700 Size: 110 2526 187 2869 Fragments arranged by size: 2869 2526 187 110 BsrGI T'GTAC_A Cuts at: 96 2457 96 Size: 2361 3331 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5692 BstBI TT'CG_AA Cuts at: 2954 4496 2954 Size: 1542 4150 BstEII G'GTnAC_C Cuts at: 1184 2489 1184 Size: 1305 4387 BstXI CCAn_nnnn'nTGG Cuts at: 2251 2863 2251 Size: 612 5080 BstYI r'GATC_y Cuts at: 1972 2008 2921 3022 3389 3774 3791 5243 5329 Size: 36 913 101 367 385 17 1452 86 Cuts at: 5329 5341 5687 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 913 385 367 346 101 86 36 17 12 BstZ17I GTA'TAC Cuts at: 2209 2209 Size: 5692 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4201 Fragments arranged by size: 4201 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 3208 4002 4022 3208 Size: 794 20 4878 Fragments arranged by size: 4878 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2726 2761 529 Size: 35 2162 35 3460 Fragments arranged by size: 3460 2162 35 35 DraI TTT'AAA Cuts at: 2947 2996 2947 Size: 49 5643 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3139 4028 5657 Size: 54 1425 50 275 18 1309 889 1629 Cuts at: 5657 8 Size: 43 Fragments arranged by size: 1629 1425 1309 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3139 1537 Size: 275 1327 4090 Fragments arranged by size: 4090 1327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2868 3622 1353 Size: 1515 754 3423 Fragments arranged by size: 3423 1515 754 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4279 4653 4799 387 Size: 1064 138 2690 374 146 1280 Fragments arranged by size: 2690 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2288 2395 3755 4352 5139 5407 Size: 393 470 186 107 1360 597 787 268 Cuts at: 5407 1239 Size: 1524 Fragments arranged by size: 1524 1360 787 597 470 393 268 186 107 EcoO109I rG'GnC_Cy Cuts at: 2716 2716 Size: 5692 EcoRI G'AATT_C Cuts at: 2383 2533 3001 3379 2383 Size: 150 468 378 4696 Fragments arranged by size: 4696 468 378 150 FspI TGC'GCA Cuts at: 4198 4198 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 4839 1678 Size: 3161 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2889 2921 4333 4365 4407 Size: 32 135 32 1317 32 1412 32 42 Cuts at: 4407 4439 1373 Size: 32 2626 Fragments arranged by size: 2626 1412 1317 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2417 3036 3282 669 Size: 988 281 479 619 246 3079 Fragments arranged by size: 3079 988 619 479 281 246 HindIII A'AGCT_T Cuts at: 748 2091 748 Size: 1343 4349 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2261 2789 4806 4990 1305 Size: 956 528 2017 184 2007 Fragments arranged by size: 2017 2007 956 528 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3870 Fragments arranged by size: 3870 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2360 2965 3650 4009 4168 518 Size: 648 1194 605 685 359 159 2042 Fragments arranged by size: 2042 1194 685 648 605 359 159 MspA1I CmG'CkG Cuts at: 1606 2756 3108 3785 4933 5178 1606 Size: 1150 352 677 1148 245 2120 Fragments arranged by size: 2120 1150 1148 677 352 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4201 Fragments arranged by size: 4201 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 2988 3050 4595 1132 Size: 97 1759 62 1545 2229 Fragments arranged by size: 2229 1759 1545 97 62 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5692 PciI A'CATG_T Cuts at: 2984 4591 2984 Size: 1607 4085 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5692 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2716 2716 Size: 5692 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 2844 3262 2844 Size: 418 5274 PstI C_TGCA'G Cuts at: 830 2113 3044 830 Size: 1283 931 3478 Fragments arranged by size: 3478 1283 931 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2756 2756 Size: 5692 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 2109 2703 3040 4175 4856 5047 826 Size: 219 1064 594 337 1135 681 191 1471 Fragments arranged by size: 1471 1135 1064 681 594 337 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 3019 1872 Size: 1147 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2571 2711 3796 4697 4959 820 Size: 197 926 628 140 1085 901 262 1553 Fragments arranged by size: 1553 1085 926 901 628 262 197 140 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5692 StyI C'CwwG_G Cuts at: 513 1065 2004 2744 3131 513 Size: 552 939 740 387 3074 Fragments arranged by size: 3074 939 740 552 387 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3719 3736 3889 4074 4519 325 Size: 1255 2139 17 153 185 445 1498 Fragments arranged by size: 2139 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2457 2758 3938 Size: 274 80 33 51 1443 480 301 1180 Cuts at: 3938 5650 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1180 480 301 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 5652 5684 2000 Size: 32 3620 32 2008 Fragments arranged by size: 3620 2008 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 3017 1870 Size: 1147 4545 XmnI GAAnn'nnTTC Cuts at: 3821 3821 Size: 5692 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI