################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstYI BstZ17I CelII Cfr10I CpoI DrdI Eam1105I EarI Eco47III EcoT22I FseI FspI HaeII Hin1I HincII HindII HpaI KasI Ksp632I MaeII MamI MflI MluI MluNI MroI MscI NaeI NarI NdeI NgoMIV NotI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SapI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Tth111I Van91I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AlwNI 1223 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 508 CCTGAGG CC^T[ACGT]AGG AseI 1305 ATTAAT AT^TAAT AsnI 1305 ATTAAT AT^TAAT AvaI 52 CTCGAG C^[CT]CG[GA]G BbvCI 548 CCTCAGC CCTCAGC(-5/-2) BclI 1854 TGATCA T^GATCA BglI 37 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 418 CTGGAG CTGGAG(16/14) BseRI 421 GAGGAG GAGGAG(10/8) BsiEI 243 CGGCCG CG[GA][CT]^CG BsmAI 746 GAGAC GTCTC(1/5) BsoBI 52 CTCGAG C^[CT]CG[GA]G BspDI 1348 ATCGAT AT^CGAT BspMI 657 GCAGGT ACCTGC(4/8) BsrBI 125 CCGCTC CCGCTC(-3/-3) BssSI 95 CACGAG CACGAG(-5/-1) BstAPI 1765 GCAATTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 364 GGTGACC G^GT[ACGT]ACC BstPI 364 GGTGACC G^GT[ACGT]ACC BstXI 686 CCATGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 508 CCTGAGG CC^T[ACGT]AGG ClaI 1348 ATCGAT AT^CGAT DraII 1224 AGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 1858 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 243 CGGCCG C^GGCCG EclXI 243 CGGCCG C^GGCCG Eco52I 243 CGGCCG C^GGCCG Eco81I 508 CCTGAGG CC^T[ACGT]AGG EcoNI 1216 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1224 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoO65I 364 GGTGACC G^GT[ACGT]ACC EcoRI 1669 GAATTC G^AATTC FbaI 1854 TGATCA T^GATCA HgaI 8 GCGTC GACGC(5/10) HindIII 1445 AAGCTT A^AGCTT KspI 205 CCGCGG CCGC^GG MfeI 1766 CAATTG C^AATTG MslI 687 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1766 CAATTG C^AATTG NheI 1825 GCTAGC G^CTAGC NruI 106 TCGCGA TCG^CGA PaeR7I 52 CTCGAG C^TCGAG PpuMI 1224 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 1305 ATTAAT AT^TAAT PvuII 1205 CAGCTG CAG^CTG SacII 205 CCGCGG CCGC^GG SbfI 1552 CCTGCAGG CCTGCA^GG ScaI 1546 AGTACT AGT^ACT SfaNI 270 GCATC GCATC(5/9) Sse8387I 1552 CCTGCAGG CCTGCA^GG StuI 521 AGGCCT AGG^CCT XcmI 683 CCACCATGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 52 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### Acc65I 81 GGTACC G^GTACC 457 GGTACC Asp718I 81 GGTACC G^GTACC 457 GGTACC BanII 23 GGGCTC G[GA]GC[CT]^C 1883 GAGCCC BbsI 260 GTCTTC GAAGAC(2/6) 1255 GTCTTC BpuAI 260 GTCTTC GAAGAC(2/6) 1255 GTCTTC BsrDI 284 CATTGC GCAATG(2/0) 1608 CATTGC CfoI 6 GCGC GCG^C 16 GCGC EaeI 243 CGGCCG [CT]^GGCC[GA] 977 TGGCCG EcoRV 1162 GATATC GAT^ATC 1264 GATATC EcoT14I 40 CCATGG C^C[AT][AT]GG 692 CCATGG HhaI 6 GCGC GCG^C 16 GCGC HinP1I 6 GCGC G^CGC 16 GCGC KpnI 81 GGTACC GGTAC^C 457 GGTACC NcoI 40 CCATGG C^CATGG 692 CCATGG SmlI 52 CTCGAG C^T[CT][GA]AG 1748 CTCAAG StyI 40 CCATGG C^C[AT][AT]GG 692 CCATGG TfiI 544 GATTC G^A[AT]TC 1148 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI AvaII BanI BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I Cfr13I DdeI DpnII DraI DsaI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I